LOCUS       V003247                10397 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003247
VERSION     V003247
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10397)
  AUTHORS   Hon S, Olson DG, Holwerda EK, Lanahan AA, Murphy SJL., Maloney MI,
            Zheng T, Papanek B, Guss AM, Lynd LR.
  TITLE     The ethanol pathway from Thermoanaerobacterium saccharolyticum
            improves ethanol production in Clostridium thermocellum
  JOURNAL   Metab. Eng. 42, 175-184 (2017)
   PUBMED   28663138
REFERENCE   2  (bases 1 to 10397)
  AUTHORS   Hon S, Olson DG, Holwerda EK, Lanahan AA, Murphy SJL., Maloney MI,
            Zheng T, Papanek BA, Guss AM, Lynd LR.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2017) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 10397)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10397)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Metab. Eng.
            42, 175-184 (2017)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-MAR-2017) Thayer School of Engineering, Dartmouth College, 14
            Engineering Drive, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10397
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..92
                     /label="cat promoter"
                     /note="cat promoter"
                     /regulatory_class="promoter"
     CDS             93..740
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(824..1681)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1682..1773)
                     /label="AmpR promoter"
     rep_origin      2033..2578
                     /direction=RIGHT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             3049..4050
                     /label="repB"
                     /note="RepB replication protein"
     regulatory      4099..4307
                     /label="Clo1313_2638 promoter"
                     /note="Clo1313_2638 promoter"
                     /regulatory_class="promoter"
     CDS             4308..5507
                     /codon_start=1
                     /gene="adhA"
                     /product="AdhA"
                     /label="adhA"
                     /note="NADPH-dependent alcohol dehydrogenase"
                     /protein_id="ASK86089.1"
                     /translation="MWETKVNPSKIFELRCKNTTYFGVGSIHKIKDILENLKINGINNV
                     IFITGKGSYKTSGAWDVVRPVLEELDLKYSLYDKVGPNPTVDMIDEAAKIGRESGAKAV
                     IGIGGGSPIDTAKSVAVLLKYTDKNARELYKQKFIPDDAVPIIAINLTHGTGTEVDRFA
                     VATIPEKNYKPAIAYDCLYPMFAIDDPSLMTKLDKKQTIAVTVDALNHITEAATTLVAS
                     PYSILTAKETVRLIVRYLPAAVNDPLNIVARYYLLYASALAGISFDNGLLHLTHALEHP
                     LSAVKPEIAHGLGLGAILPAVIKAIYPATAEVLADVYSPIVPGLKGLPVEAEYVAEKVQ
                     EWLFSVGCIQKLSDFGFTKDDIPNLVKLAKTTPSLDGLLSIAPVEATESVIEKIYLKSL
                     "
     gene            4308..5507
                     /gene="adhA"
                     /label="adhA"
     CDS             5525..8104
                     /codon_start=1
                     /gene="adhE"
                     /product="AdhE"
                     /label="adhE"
                     /note="bifunctional alcohol and aldehyde dehydrogenase"
                     /protein_id="ASK86090.1"
                     /translation="MATTKTELDVQKQIDLLVSRAQEAQKKFMSYTQEQIDAIVKAMAL
                     AGVDKHVELAKMAYEETKMGVYEDKITKNLFATEYVYHDIKNEKTVGIINENIEENYME
                     VAEPIGVIAGVTPVTNPTSTTMFKCLISIKTRNPIIFSFHPKAIKCSIAAAKVMYEAAL
                     KAGAPEGCIGWIETPSIEATQLLMTHPGVSLILATGGAGMVKAAYSSGKPALGVGPGNV
                     PCYIEKSANIKRAVSDLILSKTFDNGVICASEQAVIIDEEIADEVKKLMKEYGCYFLNK
                     DEIKKLEKFAIDEQSCAMSPAVVGQPAAKIAEMAGFKVPEGTKILVAEYEGVGPKYPLS
                     REKLSPILACYTVKDYNEGIKKCEEMTEFGGLGHSAVIHSENQNVINEFARRVRTGRLI
                     VNSPSSQGAIGDIYNTNTPSLTLGCGSMGRNSTTDNVSVKNLLNIKRVVIRKDRMKWFK
                     IPPKIYFESGSLQYLCKVKRKKAFIVTDPFMVKLGFVDKVTYQLDKANIEYEIFSEVEP
                     DPSVDTVMNGVKIMNSYNPDLIIAVGDGSAIDAAKGMWLFYEYPDTEFETLRLKFADIR
                     KRAFKFPELGKKALFIAIPTTSGTGSEVTAFAVITDKKRNIKYPLADYELTPDIAIIDP
                     DLTKTVPPSVTADTGMDVLTHAIEAYVSVMASDYTDALAEKAIKIVFEYLPRAYKNGND
                     EEAREKMHNASCMAGMAFTNAFLGINHSMAHILGGKFHIPHGRANAILLPYVIRYNAEK
                     PTKFVAFPQYEYPKAAERYAEIAKFLGLPASTVEEGVESLIEAIKNLMKELNIPLTLKD
                     AGINKEQFEKEIEEMSDIAFNDQCTGTNPRMPLTKEIAEIYRKAYGA"
     gene            5525..8104
                     /gene="adhE"
                     /label="adhE"
     CDS             8122..8976
                     /codon_start=1
                     /gene="nfnA"
                     /product="NfnA"
                     /label="nfnA"
                     /note="NADH-dependent reduced ferredoxin:NADP+
                     oxidoreductase"
                     /protein_id="ASK86091.1"
                     /translation="MNEILEKKQLNPTVKMMVINAPLMAKKAKPGQFVIVRVDEKGERI
                     PLTIADYDRNKGTITIIFQEVGMSTKKLGTLNVGDRLHDFVGPLGKPVEFSKDTKRVLA
                     IGGGVGVAPLYPKVKMLNEMKVPVDSIIGGRSAEYVILEDEMKKVSENLYITTDDGTKG
                     RKGFVTDVLKELIEKDNKYDEVIAIGPLIMMKMVCNITKEYNIPTMVSMNPIMIDGTGM
                     CGGCRVTVGGETKFACVDGPAFDGLKVDFDEAMRRQNMYKDMERKVLENYEHECKLGGI
                     LNG"
     gene            8122..8976
                     /gene="nfnA"
                     /label="nfnA"
     CDS             8969..10360
                     /codon_start=1
                     /gene="nfnB"
                     /product="NfnB"
                     /label="nfnB"
                     /note="NADH-dependent reduced ferredoxin:NADP+
                     oxidoreductase"
                     /protein_id="ASK86092.1"
                     /translation="MANMSLKKVPMPEQEPDQRNKNFKEVALGYEENMAVEEAERCIQC
                     KNQPCVEGCPVHVKIPEFIKLIANRDFEGAYQKIKETNNLPAICGRVCPQESQCESVCT
                     RGKKGEPVAIGRLERFTADWHMKNNEDKIEKPETNGRKVAVIGSGPAGLSCAGDLAKMG
                     YDTTIFEAFHTPGGVLMYGIPEFRLPKEIVQKEIDSLKKLGVKIETNMVIGKILTIDDL
                     FDMGYEAVFIGTGAGLPKFMNIPGENLNGVYSANEFLTRINLMKAYDFPNSPTPVKVGK
                     KVAVVGGGNVAMDAARSAKRMGAEEVYIVYRRSEEEMPARLEEIHHAKEEGIIFKLLTN
                     PVRIIGDESGSVKGIECVNMVLGDVDESGRRRPVEEKGSEHVIDVDTVIIAIGQSPNPL
                     ITSTTEGLEKQRWGGIIVNEETLETSRRGVFAGGDAVTGAATVILAMGAGKKAAASIHK
                     YLSEK"
     gene            8969..10360
                     /gene="nfnB"
                     /label="nfnB"
ORIGIN
        1 ttgactttta aaaaaggatt gattctaatg aagaaagcag acaagtaagc ctcctaaatt
       61 cactttagat aaaaatttag gaggcatatc aaatgaactt taataaaatt gatttagaca
      121 attggaagag aaaagagata tttaatcatt atttgaacca acaaacgact tttagtataa
      181 ccacagaaat tgatattagt gttttatacc gaaacataaa acaagaagga tataaatttt
      241 accctgcatt tattttctta gtgacaaggg tgataaactc aaatacagct tttagaactg
      301 gttacaatag cgacggagag ttaggttatt gggataagtt agagccactt tatacaattt
      361 ttgatggtgt atctaaaaca ttctctggta tttggactcc tgtaaagaat gacttcaaag
      421 agttttatga tttatacctt tctgatgtag agaaatataa tggttcgggg aaattgtttc
      481 ccaaaacacc tatacctgaa aatgcttttt ctctttctat tattccatgg acttcattta
      541 ctgggtttaa cttaaatatc aataataata gtaattacct tctacccatt attacagcag
      601 gaaaattcat taataaaggt aattcaatat atttaccgct atctttacag gtacatcatt
      661 ctgtttgtga tggttatcat gcaggattgt ttatgaactc tattcaggaa ttgtcagata
      721 ggcctaatga ctggctttta taatatgaga taatgccgac tgtacttttt acagtcggtt
      781 ttctaatgtc actagggctc gcctttggga agtttgaagg ttaccaatgc ttaatcagtg
      841 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
      901 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
      961 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1021 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1081 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag
     1141 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1201 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1261 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1321 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1381 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     1441 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1501 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1561 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1621 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1681 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1741 acatatttga atgtatttag aaaaataaac aaaagagttt gaggaaaact taagaaaacg
     1801 ggaaactatt tcctgttgag aatgtgaaat taaatgataa aaagtgatat aatagggtca
     1861 ggcgtacaga agcctgacct ttttgcaaaa ttgttttatt ggcgcggcgt atatatacag
     1921 catatataca atgtaaattt atacaatcaa attgttgata caatcgaatt atgcggctaa
     1981 aaatttgctc atgagcctag aaatatttta tctgattaat aagatgatct tcttgagatc
     2041 gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg cagggcggtt
     2101 tttcgaaggt tctctgagct accaactctt tgaaccgagg taactggctt ggaggagcgc
     2161 agtcaccaaa acttgtcctt tcagtttagc cttaaccggc gcatgacttc aagactaact
     2221 cctctaaatc aattaccagt ggctgctgcc agtggtgctt ttgcatgtct ttccgggttg
     2281 gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg gggttcgtgc
     2341 atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg tggaatgaga
     2401 caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa caggagagcg
     2461 cacgagggag ccgccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2521 ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc tatggaaaaa
     2581 cggctttgcc gcggccctct cacttccctg ttaagtatct tcctggcatc ttccaggaaa
     2641 tctccgcccc gttcgtaagc catttccgct cgccgcagtc gaacgaccga gcgtagcgag
     2701 tcagtgagcg aggaagcgga atatatcctg tatcacatat tctgctgacg caccggtgca
     2761 gccttttttc tcctgccaca tgaagcactt cactgacacc ctcatcagtg ccaacatagt
     2821 aagccagtat acaaatgagt gctttttttg cgttttgagc gtagcgaaaa acgagttctt
     2881 tctattcttg atacatatag aaataacgtc atttttattt tagttgctga aaggtgcgtt
     2941 gaagtgttgg tatgtatgtg ttttaaagta ttgaaaaccc ttaaaattgg ttgcacagaa
     3001 aaaccccatc tgttaaagtt ataagtgacc aaacaaataa ctaaatagat gggggtttct
     3061 tttaatatta tgtgtcctaa tagtagcatt tattcagatg aaaaatcaag ggttttagtg
     3121 gacaagacaa aaagtggaaa agtgagacca tggagagaaa agaaaatcgc taatgttgat
     3181 tactttgaac ttctgcatat tcttgaattt aaaaaggctg aaagagtaaa agattgtgct
     3241 gaaatattag agtataaaca aaatcgtgaa acaggcgaaa gaaagttgta tcgagtgtgg
     3301 ttttgtaaat ccaggctttg tccaatgtgc aactggagga gagcaatgaa acatggcatt
     3361 cagtcacaaa aggttgttgc tgaagttatt aaacaaaagc caacagttcg ttggttgttt
     3421 ctcacattaa cagttaaaaa tgtttatgat ggcgaagaat taaataagag tttgtcagat
     3481 atggctcaag gatttcgccg aatgatgcaa tataaaaaaa ttaataaaaa tcttgttggt
     3541 tttatgcgtg caacggaagt gacaataaat aataaagata attcttataa tcagcacatg
     3601 catgtattgg tatgtgtgga accaacttat tttaagaata cagaaaacta cgtgaatcaa
     3661 aaacaatgga ttcaattttg gaaaaaggca atgaaattag actatgatcc aaatgtaaaa
     3721 gttcaaatga ttcgaccgaa aaataaatat aaatcggata tacaatcggc aattgacgaa
     3781 actgcaaaat atcctgtaaa ggatacggat tttatgaccg atgatgaaga aaagaatttg
     3841 aaacgtttgt ctgatttgga ggaaggttta caccgtaaaa ggttaatctc ctatggtggt
     3901 ttgttaaaag aaatacataa aaaattaaac cttgatgaca cagaagaagg cgatttgatt
     3961 catacagatg atgacgaaaa agccgatgaa gatggatttt ctattattgc aatgtggaat
     4021 tgggaacgga aaaattattt tattaaagag tagttcaacc cggggatcct ctagagtcga
     4081 cctgcaggca tgcaagctga taaacaaagg acggttcagg gcttctgctc atcctactct
     4141 gcattgtaaa aaggtaggat gaatttttat ttttaatctt attgaaaaaa atttttgaaa
     4201 atcggtttta ttaaaaaaaa gtgggtatat ttataatagt caattgattg gttaaaaaaa
     4261 tttaaataag caaacagaat aataacaaaa gtaaggagga atttgttatg tgggaaacaa
     4321 aagtaaatcc tagcaaaatt tttgaacttc gctgtaaaaa tacgacttat tttggtgttg
     4381 gcagtataca taagataaag gatatattag aaaatctaaa aattaatggc attaataatg
     4441 ttatttttat aacaggcaaa ggttcgtata agactagcgg agcttgggat gttgtaaggc
     4501 ctgtattgga agagcttgat ttaaaatatt cactttacga taaagttggt ccaaatccta
     4561 ccgttgatat gatagacgaa gcggcaaaaa ttggacgaga atctggagcg aaagccgtaa
     4621 ttggaattgg cggtggtagc cctatagata ctgcaaaaag tgtagctgtg ctattaaagt
     4681 atactgataa aaatgcacga gagctttata agcaaaaatt tattcctgat gatgctgttc
     4741 ctataatagc tataaatctt acacatggta caggaacaga ggtagataga tttgcagtgg
     4801 ctactatacc tgagaaaaat tataaaccag ctatagctta tgattgctta tatcctatgt
     4861 ttgctataga tgacccttcg cttatgacaa agcttgataa aaaacagact atagccgtta
     4921 ctgttgatgc tttaaatcac attacagaag ctgctacgac acttgttgca tctccatata
     4981 gtattcttac ggctaaggaa acagtaagat tgattgttcg atatcttccc gctgctgtaa
     5041 atgatccttt aaatatagtg gcaagatatt accttctgta tgcttctgca ttagctggca
     5101 tatcttttga caatggtctt ttgcatctta ctcatgcttt agagcatcct ttaagtgctg
     5161 taaaacctga aatagctcat ggtttagggc ttggtgcaat attgccagca gttataaaag
     5221 ctatttatcc agctacagca gaagtattgg ctgatgtata tagtcctata gttcctggtt
     5281 taaaaggact gcctgttgag gcggagtatg tagcagaaaa agttcaagaa tggcttttta
     5341 gtgtggggtg cattcaaaag ttatctgatt ttgggtttac gaaagatgat attcctaatc
     5401 ttgtaaaact tgctaaaaca actccttccc ttgatgggtt gttatctatt gcacctgttg
     5461 aagctactga atctgtcatt gaaaagattt atttaaaatc tctttaataa aggaggtcga
     5521 cgtcatggca acgacaaaaa cggaattaga cgttcagaag cagatagatc tacttgtgtc
     5581 aagagcacaa gaggctcaga aaaaattcat gtcttacacg caagagcaaa tcgacgcaat
     5641 agttaaggca atggctttag caggcgttga caaacacgta gagctggcaa agatggcgta
     5701 cgaagagaca aaaatgggtg tatacgaaga taagataaca aaaaatctct tcgcaacaga
     5761 gtacgtgtac cacgacataa aaaatgaaaa gactgtagga atcataaacg agaacataga
     5821 agaaaactac atggaagtgg cagaaccgat aggcgtaatt gccggtgtca cacctgtcac
     5881 aaacccaaca tctaccacga tgtttaaatg cttaatatcc ataaagacgc gaaatcctat
     5941 aatattcagc ttccatccaa aggcaataaa gtgcagcatc gcagcagcca aagtgatgta
     6001 tgaagctgca ctaaaggcag gcgcacctga aggatgcata ggatggatag aaacgccatc
     6061 aattgaggcc acacagcttc tcatgacaca tccaggcgta tcgctgatcc ttgcaacggg
     6121 cggtgcagga atggtaaaag cggcatacag ctcaggaaaa ccggcattag gcgtaggtcc
     6181 tggcaatgtg ccatgctaca tcgaaaaatc agcaaacata aagagggctg tatcggatct
     6241 catactaagc aagacatttg acaatggagt aatatgcgca tcagagcagg ccgtaataat
     6301 agacgaggaa atagcagatg aagtcaaaaa gcttatgaaa gaatacggct gctacttctt
     6361 aaacaaagat gaaataaaga agcttgagaa atttgcaatt gatgagcaaa gctgcgccat
     6421 gagccctgca gtggtaggtc agccagcggc gaagattgct gaaatggcag gcttcaaagt
     6481 ccccgaaggc acaaagatat tagtggcaga gtacgaagga gtaggtccaa aatatcctct
     6541 atcaagggag aaactaagcc cgattcttgc ttgctacacc gtcaaagact acaatgaagg
     6601 aatcaaaaag tgcgaggaaa tgactgaatt cggaggttta ggccactctg ctgtaataca
     6661 ctctgaaaat caaaacgtca taaatgaatt tgcaaggcga gtccgcacag gaagacttat
     6721 cgtaaattca ccatcatcac agggagcaat aggagatata tacaatacaa acacgccatc
     6781 acttacatta ggctgtggtt ctatgggaag aaactcaacg acagacaatg taagcgtcaa
     6841 gaaccttttg aatattaagc gtgtcgtgat aaggaaggat agaatgaaat ggttcaagat
     6901 tccaccgaag atttactttg aaagcgggtc actccagtac ctgtgcaaag tcaaaagaaa
     6961 aaaagcgttt atcgtcacag atccattcat ggttaagctt ggcttcgtag acaaagtgac
     7021 atatcaatta gacaaagcaa acatcgaata cgaaatattc tcagaagtag agccagatcc
     7081 atctgttgac acagtcatga acggcgtaaa aataatgaat tcgtacaatc ctgacttaat
     7141 aatcgctgta ggcgatggct ctgcaataga cgcagcaaag ggaatgtggc ttttctacga
     7201 atatcctgat acagagtttg aaacattgag gcttaaattt gcagacatca gaaaaagggc
     7261 atttaagttc ccagaacttg gcaaaaaagc gctattcatc gcaataccga caacaagcgg
     7321 cacaggctca gaagtgacag catttgccgt aataaccgac aaaaagagaa acatcaagta
     7381 tccactggca gactacgaac ttacacctga catagccata atagatcctg accttacaaa
     7441 gactgtaccg ccatctgtaa cagcagacac aggcatggat gtgctgacac acgccataga
     7501 agcatacgta tcagtaatgg catcagacta cacagatgca ctggcggaaa aggctataaa
     7561 gatcgtattt gaatacctgc caagggctta taaaaacggc aatgatgaag aagcccgcga
     7621 aaagatgcac aatgcttcct gcatggctgg tatggcattc acaaatgcat tcttaggaat
     7681 aaaccacagc atggcacaca tactgggcgg aaagttccac ataccacacg gaagagcaaa
     7741 tgcaatactt ctgccgtatg taataaggta caatgcagaa aaacctacaa agtttgtggc
     7801 attcccacaa tacgaatatc caaaagcagc agaaagatat gcggaaatcg ccaaattctt
     7861 aggactgcct gcttcaactg ttgaagaagg cgtagaaagc ttaatagaag ctataaagaa
     7921 cctcatgaaa gagcttaaca ttccgcttac acttaaagac gccggcatca acaaagaaca
     7981 gtttgaaaaa gaaatagagg aaatgtcaga catcgccttc aacgatcagt gcacagggac
     8041 aaacccgaga atgcctctca caaaagaaat tgcagagatc tacagaaaag catacggtgc
     8101 atagtaaagg aggttataaa aatgaacgag attttagaga agaagcaatt gaatcccact
     8161 gtcaaaatga tggttataaa cgctccacta atggcgaaaa aggcgaaacc tggacagttt
     8221 gtcatagtaa gagtagatga aaagggtgaa cgcattcctt tgacaatagc ggattacgac
     8281 agaaacaaag gcacgattac tataatattt caagaagtcg gcatgagcac caagaagtta
     8341 ggtacgttaa acgtaggcga tagattgcat gacttcgtag gacctttagg aaaacctgtt
     8401 gaattttcaa aagatacgaa gagagtttta gcaatcggcg gaggtgttgg agttgcgccg
     8461 ctttatccaa aagtaaaaat gctaaatgag atgaaagtac cagtggatag tataataggc
     8521 ggcagaagtg cagagtacgt catattagaa gatgaaatga aaaaagtcag tgaaaacctt
     8581 tatataacga cggatgacgg cacaaaaggc cgtaaaggct ttgtaacaga tgtattaaaa
     8641 gaattgatag aaaaagacaa caaatacgat gaagtaattg caattgggcc actgataatg
     8701 atgaagatgg tatgcaatat aacaaaagaa tacaacatac caactatggt cagcatgaac
     8761 ccgataatga tagacggaac tggaatgtgc ggaggatgca gagtcacagt tggaggagag
     8821 acaaaatttg catgtgtcga tggtccagca tttgacgggc ttaaagtaga ctttgatgaa
     8881 gcaatgagaa ggcagaatat gtataaagat atggagagga aagtattaga aaattatgag
     8941 cacgaatgca agttaggggg catcttaaat ggctaacatg tcgttaaaaa aagtaccaat
     9001 gcctgaacag gaaccagatc agcggaacaa aaatttcaaa gaagttgcat tagggtatga
     9061 agaaaacatg gctgttgaag aagccgaaag atgtattcaa tgtaagaatc agccatgtgt
     9121 tgaaggttgc cctgttcacg taaaaatacc tgagttcatc aagctgatag caaacaggga
     9181 ctttgaagga gcatatcaaa agataaaaga aacgaataat ctacctgcaa tatgtggaag
     9241 agtatgtcca caggaaagcc agtgtgaatc agtatgcaca agaggcaaaa aaggagaacc
     9301 tgtagctata ggcagactgg agagatttac agcagattgg catatgaaaa ataatgaaga
     9361 taaaatagaa aaaccagaga cgaatggcag aaaagttgct gttataggat ctgggcctgc
     9421 aggactatcg tgtgcaggtg atcttgcaaa aatgggatat gacacaacca tctttgaagc
     9481 atttcataca ccaggcggtg ttttaatgta cggtatccca gagtttagac ttccaaaaga
     9541 aatagtgcaa aaagaaattg attcgttgaa aaaattggga gtaaaaatag aaaccaacat
     9601 ggtcataggc aagatactta caatcgatga tttattcgac atggggtatg aagcagtgtt
     9661 catagggaca ggcgcaggat tgcctaaatt tatgaatata cctggagaaa acttaaatgg
     9721 agtttactca gcaaatgaat ttttgacgag gattaatttg atgaaagctt atgatttccc
     9781 taacagtccc actcctgtaa aagtaggcaa gaaagttgct gttgttggcg gtggaaacgt
     9841 ggcgatggat gcagcaagat ccgcaaagcg catgggagca gaagaagtgt acatcgtata
     9901 tagaaggtca gaggaagaaa tgcctgcaag gcttgaggaa atacaccacg caaaagaaga
     9961 aggaatcata tttaagttat tgacaaaccc tgtaagaatc attggtgatg aaagtggcag
    10021 tgtaaaagga atcgaatgtg ttaacatggt tttaggcgat gtagacgaat caggtagaag
    10081 aagacctgta gaagaaaaag gctcagagca tgtaatagat gtagatacag ttattatagc
    10141 aataggacaa agtccaaatc cgcttataac atccacaaca gaaggtttag aaaagcaaag
    10201 atggggtgga ataatagtca acgaagaaac attggagaca agccgcagag gagtttttgc
    10261 gggcggtgat gctgtgacag gtgcagctac agtaatactt gcaatgggag ctggtaaaaa
    10321 agctgcagct tctatacaca aatatttaag cgaaaagtga ttaatagaaa taattttgtt
    10381 taactttaca aacggga
//
