LOCUS       V003249                10397 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003249
VERSION     V003249
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10397)
  AUTHORS   Hon S, Olson DG, Holwerda EK, Lanahan AA, Murphy SJL., Maloney MI,
            Zheng T, Papanek B, Guss AM, Lynd LR.
  TITLE     The ethanol pathway from Thermoanaerobacterium saccharolyticum
            improves ethanol production in Clostridium thermocellum
  JOURNAL   Metab. Eng. 42, 175-184 (2017)
   PUBMED   28663138
REFERENCE   2  (bases 1 to 10397)
  AUTHORS   Hon S, Olson DG, Holwerda EK, Lanahan AA, Murphy SJL., Maloney MI,
            Zheng T, Papanek BA, Guss AM, Lynd LR.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2017) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 10397)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10397)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Metab. Eng.
            42, 175-184 (2017)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-MAR-2017) Thayer School of Engineering, Dartmouth College, 14
            Engineering Drive, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10397
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..92
                     /label="cat promoter"
                     /note="cat promoter"
                     /regulatory_class="promoter"
     CDS             93..740
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(824..1681)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1682..1773)
                     /label="AmpR promoter"
     rep_origin      2033..2578
                     /direction=RIGHT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             3049..4050
                     /label="repB"
                     /note="RepB replication protein"
     regulatory      4099..4307
                     /label="Clo1313_2638 promoter"
                     /note="Clo1313_2638 promoter"
                     /regulatory_class="promoter"
     CDS             4308..5162
                     /codon_start=1
                     /gene="nfnA"
                     /product="NfnA"
                     /label="nfnA"
                     /note="NADH-dependent reduced ferredoxin:NADP+
                     oxidoreductase"
                     /protein_id="ASK86077.1"
                     /translation="MNEILEKKQLNPTVKMMVINAPLMAKKAKPGQFVIVRVDEKGERI
                     PLTIADYDRNKGTITIIFQEVGMSTKKLGTLNVGDRLHDFVGPLGKPVEFSKDTKRVLA
                     IGGGVGVAPLYPKVKMLNEMKVPVDSIIGGRSAEYVILEDEMKKVSENLYITTDDGTKG
                     RKGFVTDVLKELIEKDNKYDEVIAIGPLIMMKMVCNITKEYNIPTMVSMNPIMIDGTGM
                     CGGCRVTVGGETKFACVDGPAFDGLKVDFDEAMRRQNMYKDMERKVLENYEHECKLGGI
                     LNG"
     gene            4308..5162
                     /gene="nfnA"
                     /label="nfnA"
     CDS             5155..6546
                     /codon_start=1
                     /gene="nfnB"
                     /product="NfnB"
                     /label="nfnB"
                     /note="NADH-dependent reduced ferredoxin:NADP+
                     oxidoreductase"
                     /protein_id="ASK86078.1"
                     /translation="MANMSLKKVPMPEQEPDQRNKNFKEVALGYEENMAVEEAERCIQC
                     KNQPCVEGCPVHVKIPEFIKLIANRDFEGAYQKIKETNNLPAICGRVCPQESQCESVCT
                     RGKKGEPVAIGRLERFTADWHMKNNEDKIEKPETNGRKVAVIGSGPAGLSCAGDLAKMG
                     YDTTIFEAFHTPGGVLMYGIPEFRLPKEIVQKEIDSLKKLGVKIETNMVIGKILTIDDL
                     FDMGYEAVFIGTGAGLPKFMNIPGENLNGVYSANEFLTRINLMKAYDFPNSPTPVKVGK
                     KVAVVGGGNVAMDAARSAKRMGAEEVYIVYRRSEEEMPARLEEIHHAKEEGIIFKLLTN
                     PVRIIGDESGSVKGIECVNMVLGDVDESGRRRPVEEKGSEHVIDVDTVIIAIGQSPNPL
                     ITSTTEGLEKQRWGGIIVNEETLETSRRGVFAGGDAVTGAATVILAMGAGKKAAASIHK
                     YLSEK"
     gene            5155..6546
                     /gene="nfnB"
                     /label="nfnB"
     CDS             6564..9143
                     /codon_start=1
                     /gene="adhE"
                     /product="AdhE"
                     /label="adhE"
                     /note="bifunctional alcohol and aldehyde dehydrogenase"
                     /protein_id="ASK86075.1"
                     /translation="MATTKTELDVQKQIDLLVSRAQEAQKKFMSYTQEQIDAIVKAMAL
                     AGVDKHVELAKMAYEETKMGVYEDKITKNLFATEYVYHDIKNEKTVGIINENIEENYME
                     VAEPIGVIAGVTPVTNPTSTTMFKCLISIKTRNPIIFSFHPKAIKCSIAAAKVMYEAAL
                     KAGAPEGCIGWIETPSIEATQLLMTHPGVSLILATGGAGMVKAAYSSGKPALGVGPGNV
                     PCYIEKSANIKRAVSDLILSKTFDNGVICASEQAVIIDEEIADEVKKLMKEYGCYFLNK
                     DEIKKLEKFAIDEQSCAMSPAVVGQPAAKIAEMAGFKVPEGTKILVAEYEGVGPKYPLS
                     REKLSPILACYTVKDYNEGIKKCEEMTEFGGLGHSAVIHSENQNVINEFARRVRTGRLI
                     VNSPSSQGAIGDIYNTNTPSLTLGCGSMGRNSTTDNVSVKNLLNIKRVVIRKDRMKWFK
                     IPPKIYFESGSLQYLCKVKRKKAFIVTDPFMVKLGFVDKVTYQLDKANIEYEIFSEVEP
                     DPSVDTVMNGVKIMNSYNPDLIIAVGDGSAIDAAKGMWLFYEYPDTEFETLRLKFADIR
                     KRAFKFPELGKKALFIAIPTTSGTGSEVTAFAVITDKKRNIKYPLADYELTPDIAIIDP
                     DLTKTVPPSVTADTGMDVLTHAIEAYVSVMASDYTDALAEKAIKIVFEYLPRAYKNGND
                     EEAREKMHNASCMAGMAFTNAFLGINHSMAHILGGKFHIPHGRANAILLPYVIRYNAEK
                     PTKFVAFPQYEYPKAAERYAEIAKFLGLPASTVEEGVESLIEAIKNLMKELNIPLTLKD
                     AGINKEQFEKEIEEMSDIAFNDQCTGTNPRMPLTKEIAEIYRKAYGA"
     gene            6564..9143
                     /gene="adhE"
                     /label="adhE"
     CDS             9161..10360
                     /codon_start=1
                     /gene="adhA"
                     /product="AdhA"
                     /label="adhA"
                     /note="NADPH-dependent alcohol dehydrogenase"
                     /protein_id="ASK86076.1"
                     /translation="MWETKVNPSKIFELRCKNTTYFGVGSIHKIKDILENLKINGINNV
                     IFITGKGSYKTSGAWDVVRPVLEELDLKYSLYDKVGPNPTVDMIDEAAKIGRESGAKAV
                     IGIGGGSPIDTAKSVAVLLKYTDKNARELYKQKFIPDDAVPIIAINLTHGTGTEVDRFA
                     VATIPEKNYKPAIAYDCLYPMFAIDDPSLMTKLDKKQTIAVTVDALNHITEAATTLVAS
                     PYSILTAKETVRLIVRYLPAAVNDPLNIVARYYLLYASALAGISFDNGLLHLTHALEHP
                     LSAVKPEIAHGLGLGAILPAVIKAIYPATAEVLADVYSPIVPGLKGLPVEAEYVAEKVQ
                     EWLFSVGCIQKLSDFGFTKDDIPNLVKLAKTTPSLDGLLSIAPVEATESVIEKIYLKSL
                     "
     gene            9161..10360
                     /gene="adhA"
                     /label="adhA"
ORIGIN
        1 ttgactttta aaaaaggatt gattctaatg aagaaagcag acaagtaagc ctcctaaatt
       61 cactttagat aaaaatttag gaggcatatc aaatgaactt taataaaatt gatttagaca
      121 attggaagag aaaagagata tttaatcatt atttgaacca acaaacgact tttagtataa
      181 ccacagaaat tgatattagt gttttatacc gaaacataaa acaagaagga tataaatttt
      241 accctgcatt tattttctta gtgacaaggg tgataaactc aaatacagct tttagaactg
      301 gttacaatag cgacggagag ttaggttatt gggataagtt agagccactt tatacaattt
      361 ttgatggtgt atctaaaaca ttctctggta tttggactcc tgtaaagaat gacttcaaag
      421 agttttatga tttatacctt tctgatgtag agaaatataa tggttcgggg aaattgtttc
      481 ccaaaacacc tatacctgaa aatgcttttt ctctttctat tattccatgg acttcattta
      541 ctgggtttaa cttaaatatc aataataata gtaattacct tctacccatt attacagcag
      601 gaaaattcat taataaaggt aattcaatat atttaccgct atctttacag gtacatcatt
      661 ctgtttgtga tggttatcat gcaggattgt ttatgaactc tattcaggaa ttgtcagata
      721 ggcctaatga ctggctttta taatatgaga taatgccgac tgtacttttt acagtcggtt
      781 ttctaatgtc actagggctc gcctttggga agtttgaagg ttaccaatgc ttaatcagtg
      841 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
      901 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
      961 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1021 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1081 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag
     1141 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1201 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1261 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1321 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1381 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     1441 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1501 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1561 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1621 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1681 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1741 acatatttga atgtatttag aaaaataaac aaaagagttt gaggaaaact taagaaaacg
     1801 ggaaactatt tcctgttgag aatgtgaaat taaatgataa aaagtgatat aatagggtca
     1861 ggcgtacaga agcctgacct ttttgcaaaa ttgttttatt ggcgcggcgt atatatacag
     1921 catatataca atgtaaattt atacaatcaa attgttgata caatcgaatt atgcggctaa
     1981 aaatttgctc atgagcctag aaatatttta tctgattaat aagatgatct tcttgagatc
     2041 gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg cagggcggtt
     2101 tttcgaaggt tctctgagct accaactctt tgaaccgagg taactggctt ggaggagcgc
     2161 agtcaccaaa acttgtcctt tcagtttagc cttaaccggc gcatgacttc aagactaact
     2221 cctctaaatc aattaccagt ggctgctgcc agtggtgctt ttgcatgtct ttccgggttg
     2281 gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg gggttcgtgc
     2341 atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg tggaatgaga
     2401 caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa caggagagcg
     2461 cacgagggag ccgccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2521 ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc tatggaaaaa
     2581 cggctttgcc gcggccctct cacttccctg ttaagtatct tcctggcatc ttccaggaaa
     2641 tctccgcccc gttcgtaagc catttccgct cgccgcagtc gaacgaccga gcgtagcgag
     2701 tcagtgagcg aggaagcgga atatatcctg tatcacatat tctgctgacg caccggtgca
     2761 gccttttttc tcctgccaca tgaagcactt cactgacacc ctcatcagtg ccaacatagt
     2821 aagccagtat acaaatgagt gctttttttg cgttttgagc gtagcgaaaa acgagttctt
     2881 tctattcttg atacatatag aaataacgtc atttttattt tagttgctga aaggtgcgtt
     2941 gaagtgttgg tatgtatgtg ttttaaagta ttgaaaaccc ttaaaattgg ttgcacagaa
     3001 aaaccccatc tgttaaagtt ataagtgacc aaacaaataa ctaaatagat gggggtttct
     3061 tttaatatta tgtgtcctaa tagtagcatt tattcagatg aaaaatcaag ggttttagtg
     3121 gacaagacaa aaagtggaaa agtgagacca tggagagaaa agaaaatcgc taatgttgat
     3181 tactttgaac ttctgcatat tcttgaattt aaaaaggctg aaagagtaaa agattgtgct
     3241 gaaatattag agtataaaca aaatcgtgaa acaggcgaaa gaaagttgta tcgagtgtgg
     3301 ttttgtaaat ccaggctttg tccaatgtgc aactggagga gagcaatgaa acatggcatt
     3361 cagtcacaaa aggttgttgc tgaagttatt aaacaaaagc caacagttcg ttggttgttt
     3421 ctcacattaa cagttaaaaa tgtttatgat ggcgaagaat taaataagag tttgtcagat
     3481 atggctcaag gatttcgccg aatgatgcaa tataaaaaaa ttaataaaaa tcttgttggt
     3541 tttatgcgtg caacggaagt gacaataaat aataaagata attcttataa tcagcacatg
     3601 catgtattgg tatgtgtgga accaacttat tttaagaata cagaaaacta cgtgaatcaa
     3661 aaacaatgga ttcaattttg gaaaaaggca atgaaattag actatgatcc aaatgtaaaa
     3721 gttcaaatga ttcgaccgaa aaataaatat aaatcggata tacaatcggc aattgacgaa
     3781 actgcaaaat atcctgtaaa ggatacggat tttatgaccg atgatgaaga aaagaatttg
     3841 aaacgtttgt ctgatttgga ggaaggttta caccgtaaaa ggttaatctc ctatggtggt
     3901 ttgttaaaag aaatacataa aaaattaaac cttgatgaca cagaagaagg cgatttgatt
     3961 catacagatg atgacgaaaa agccgatgaa gatggatttt ctattattgc aatgtggaat
     4021 tgggaacgga aaaattattt tattaaagag tagttcaacc cggggatcct ctagagtcga
     4081 cctgcaggca tgcaagctga taaacaaagg acggttcagg gcttctgctc atcctactct
     4141 gcattgtaaa aaggtaggat gaatttttat ttttaatctt attgaaaaaa atttttgaaa
     4201 atcggtttta ttaaaaaaaa gtgggtatat ttataatagt caattgattg gttaaaaaaa
     4261 tttaaataag caaacagaat aataacaaaa gtaaggagga atttgttatg aacgagattt
     4321 tagagaagaa gcaattgaat cccactgtca aaatgatggt tataaacgct ccactaatgg
     4381 cgaaaaaggc gaaacctgga cagtttgtca tagtaagagt agatgaaaag ggtgaacgca
     4441 ttcctttgac aatagcggat tacgacagaa acaaaggcac gattactata atatttcaag
     4501 aagtcggcat gagcaccaag aagttaggta cgttaaacgt aggcgataga ttgcatgact
     4561 tcgtaggacc tttaggaaaa cctgttgaat tttcaaaaga tacgaagaga gttttagcaa
     4621 tcggcggagg tgttggagtt gcgccgcttt atccaaaagt aaaaatgcta aatgagatga
     4681 aagtaccagt ggatagtata ataggcggca gaagtgcaga gtacgtcata ttagaagatg
     4741 aaatgaaaaa agtcagtgaa aacctttata taacgacgga tgacggcaca aaaggccgta
     4801 aaggctttgt aacagatgta ttaaaagaat tgatagaaaa agacaacaaa tacgatgaag
     4861 taattgcaat tgggccactg ataatgatga agatggtatg caatataaca aaagaataca
     4921 acataccaac tatggtcagc atgaacccga taatgataga cggaactgga atgtgcggag
     4981 gatgcagagt cacagttgga ggagagacaa aatttgcatg tgtcgatggt ccagcatttg
     5041 acgggcttaa agtagacttt gatgaagcaa tgagaaggca gaatatgtat aaagatatgg
     5101 agaggaaagt attagaaaat tatgagcacg aatgcaagtt agggggcatc ttaaatggct
     5161 aacatgtcgt taaaaaaagt accaatgcct gaacaggaac cagatcagcg gaacaaaaat
     5221 ttcaaagaag ttgcattagg gtatgaagaa aacatggctg ttgaagaagc cgaaagatgt
     5281 attcaatgta agaatcagcc atgtgttgaa ggttgccctg ttcacgtaaa aatacctgag
     5341 ttcatcaagc tgatagcaaa cagggacttt gaaggagcat atcaaaagat aaaagaaacg
     5401 aataatctac ctgcaatatg tggaagagta tgtccacagg aaagccagtg tgaatcagta
     5461 tgcacaagag gcaaaaaagg agaacctgta gctataggca gactggagag atttacagca
     5521 gattggcata tgaaaaataa tgaagataaa atagaaaaac cagagacgaa tggcagaaaa
     5581 gttgctgtta taggatctgg gcctgcagga ctatcgtgtg caggtgatct tgcaaaaatg
     5641 ggatatgaca caaccatctt tgaagcattt catacaccag gcggtgtttt aatgtacggt
     5701 atcccagagt ttagacttcc aaaagaaata gtgcaaaaag aaattgattc gttgaaaaaa
     5761 ttgggagtaa aaatagaaac caacatggtc ataggcaaga tacttacaat cgatgattta
     5821 ttcgacatgg ggtatgaagc agtgttcata gggacaggcg caggattgcc taaatttatg
     5881 aatatacctg gagaaaactt aaatggagtt tactcagcaa atgaattttt gacgaggatt
     5941 aatttgatga aagcttatga tttccctaac agtcccactc ctgtaaaagt aggcaagaaa
     6001 gttgctgttg ttggcggtgg aaacgtggcg atggatgcag caagatccgc aaagcgcatg
     6061 ggagcagaag aagtgtacat cgtatataga aggtcagagg aagaaatgcc tgcaaggctt
     6121 gaggaaatac accacgcaaa agaagaagga atcatattta agttattgac aaaccctgta
     6181 agaatcattg gtgatgaaag tggcagtgta aaaggaatcg aatgtgttaa catggtttta
     6241 ggcgatgtag acgaatcagg tagaagaaga cctgtagaag aaaaaggctc agagcatgta
     6301 atagatgtag atacagttat tatagcaata ggacaaagtc caaatccgct tataacatcc
     6361 acaacagaag gtttagaaaa gcaaagatgg ggtggaataa tagtcaacga agaaacattg
     6421 gagacaagcc gcagaggagt ttttgcgggc ggtgatgctg tgacaggtgc agctacagta
     6481 atacttgcaa tgggagctgg taaaaaagct gcagcttcta tacacaaata tttaagcgaa
     6541 aagtgataaa ggaggtcgac gtcatggcaa cgacaaaaac ggaattagac gttcagaagc
     6601 agatagatct acttgtgtca agagcacaag aggctcagaa aaaattcatg tcttacacgc
     6661 aagagcaaat cgacgcaata gttaaggcaa tggctttagc aggcgttgac aaacacgtag
     6721 agctggcaaa gatggcgtac gaagagacaa aaatgggtgt atacgaagat aagataacaa
     6781 aaaatctctt cgcaacagag tacgtgtacc acgacataaa aaatgaaaag actgtaggaa
     6841 tcataaacga gaacatagaa gaaaactaca tggaagtggc agaaccgata ggcgtaattg
     6901 ccggtgtcac acctgtcaca aacccaacat ctaccacgat gtttaaatgc ttaatatcca
     6961 taaagacgcg aaatcctata atattcagct tccatccaaa ggcaataaag tgcagcatcg
     7021 cagcagccaa agtgatgtat gaagctgcac taaaggcagg cgcacctgaa ggatgcatag
     7081 gatggataga aacgccatca attgaggcca cacagcttct catgacacat ccaggcgtat
     7141 cgctgatcct tgcaacgggc ggtgcaggaa tggtaaaagc ggcatacagc tcaggaaaac
     7201 cggcattagg cgtaggtcct ggcaatgtgc catgctacat cgaaaaatca gcaaacataa
     7261 agagggctgt atcggatctc atactaagca agacatttga caatggagta atatgcgcat
     7321 cagagcaggc cgtaataata gacgaggaaa tagcagatga agtcaaaaag cttatgaaag
     7381 aatacggctg ctacttctta aacaaagatg aaataaagaa gcttgagaaa tttgcaattg
     7441 atgagcaaag ctgcgccatg agccctgcag tggtaggtca gccagcggcg aagattgctg
     7501 aaatggcagg cttcaaagtc cccgaaggca caaagatatt agtggcagag tacgaaggag
     7561 taggtccaaa atatcctcta tcaagggaga aactaagccc gattcttgct tgctacaccg
     7621 tcaaagacta caatgaagga atcaaaaagt gcgaggaaat gactgaattc ggaggtttag
     7681 gccactctgc tgtaatacac tctgaaaatc aaaacgtcat aaatgaattt gcaaggcgag
     7741 tccgcacagg aagacttatc gtaaattcac catcatcaca gggagcaata ggagatatat
     7801 acaatacaaa cacgccatca cttacattag gctgtggttc tatgggaaga aactcaacga
     7861 cagacaatgt aagcgtcaag aaccttttga atattaagcg tgtcgtgata aggaaggata
     7921 gaatgaaatg gttcaagatt ccaccgaaga tttactttga aagcgggtca ctccagtacc
     7981 tgtgcaaagt caaaagaaaa aaagcgttta tcgtcacaga tccattcatg gttaagcttg
     8041 gcttcgtaga caaagtgaca tatcaattag acaaagcaaa catcgaatac gaaatattct
     8101 cagaagtaga gccagatcca tctgttgaca cagtcatgaa cggcgtaaaa ataatgaatt
     8161 cgtacaatcc tgacttaata atcgctgtag gcgatggctc tgcaatagac gcagcaaagg
     8221 gaatgtggct tttctacgaa tatcctgata cagagtttga aacattgagg cttaaatttg
     8281 cagacatcag aaaaagggca tttaagttcc cagaacttgg caaaaaagcg ctattcatcg
     8341 caataccgac aacaagcggc acaggctcag aagtgacagc atttgccgta ataaccgaca
     8401 aaaagagaaa catcaagtat ccactggcag actacgaact tacacctgac atagccataa
     8461 tagatcctga ccttacaaag actgtaccgc catctgtaac agcagacaca ggcatggatg
     8521 tgctgacaca cgccatagaa gcatacgtat cagtaatggc atcagactac acagatgcac
     8581 tggcggaaaa ggctataaag atcgtatttg aatacctgcc aagggcttat aaaaacggca
     8641 atgatgaaga agcccgcgaa aagatgcaca atgcttcctg catggctggt atggcattca
     8701 caaatgcatt cttaggaata aaccacagca tggcacacat actgggcgga aagttccaca
     8761 taccacacgg aagagcaaat gcaatacttc tgccgtatgt aataaggtac aatgcagaaa
     8821 aacctacaaa gtttgtggca ttcccacaat acgaatatcc aaaagcagca gaaagatatg
     8881 cggaaatcgc caaattctta ggactgcctg cttcaactgt tgaagaaggc gtagaaagct
     8941 taatagaagc tataaagaac ctcatgaaag agcttaacat tccgcttaca cttaaagacg
     9001 ccggcatcaa caaagaacag tttgaaaaag aaatagagga aatgtcagac atcgccttca
     9061 acgatcagtg cacagggaca aacccgagaa tgcctctcac aaaagaaatt gcagagatct
     9121 acagaaaagc atacggtgca tagtaaagga ggttataaaa atgtgggaaa caaaagtaaa
     9181 tcctagcaaa atttttgaac ttcgctgtaa aaatacgact tattttggtg ttggcagtat
     9241 acataagata aaggatatat tagaaaatct aaaaattaat ggcattaata atgttatttt
     9301 tataacaggc aaaggttcgt ataagactag cggagcttgg gatgttgtaa ggcctgtatt
     9361 ggaagagctt gatttaaaat attcacttta cgataaagtt ggtccaaatc ctaccgttga
     9421 tatgatagac gaagcggcaa aaattggacg agaatctgga gcgaaagccg taattggaat
     9481 tggcggtggt agccctatag atactgcaaa aagtgtagct gtgctattaa agtatactga
     9541 taaaaatgca cgagagcttt ataagcaaaa atttattcct gatgatgctg ttcctataat
     9601 agctataaat cttacacatg gtacaggaac agaggtagat agatttgcag tggctactat
     9661 acctgagaaa aattataaac cagctatagc ttatgattgc ttatatccta tgtttgctat
     9721 agatgaccct tcgcttatga caaagcttga taaaaaacag actatagccg ttactgttga
     9781 tgctttaaat cacattacag aagctgctac gacacttgtt gcatctccat atagtattct
     9841 tacggctaag gaaacagtaa gattgattgt tcgatatctt cccgctgctg taaatgatcc
     9901 tttaaatata gtggcaagat attaccttct gtatgcttct gcattagctg gcatatcttt
     9961 tgacaatggt cttttgcatc ttactcatgc tttagagcat cctttaagtg ctgtaaaacc
    10021 tgaaatagct catggtttag ggcttggtgc aatattgcca gcagttataa aagctattta
    10081 tccagctaca gcagaagtat tggctgatgt atatagtcct atagttcctg gtttaaaagg
    10141 actgcctgtt gaggcggagt atgtagcaga aaaagttcaa gaatggcttt ttagtgtggg
    10201 gtgcattcaa aagttatctg attttgggtt tacgaaagat gatattccta atcttgtaaa
    10261 acttgctaaa acaactcctt cccttgatgg gttgttatct attgcacctg ttgaagctac
    10321 tgaatctgtc attgaaaaga tttatttaaa atctctttaa ttaatagaaa taattttgtt
    10381 taactttaca aacggga
//
