LOCUS       V003258                10886 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003258
VERSION     V003258
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10886)
  AUTHORS   Lo J, Zheng T, Hon S, Olson DG, Lynd LR.
  TITLE     The bifunctional alcohol and aldehyde dehydrogenase gene, adhE, is
            necessary for ethanol production in Clostridium thermocellum and
            Thermoanaerobacterium saccharolyticum
  JOURNAL   J. Bacteriol. 197 (8), 1386-1393 (2015)
   PUBMED   25666131
REFERENCE   2  (bases 1 to 10886)
  AUTHORS   Zheng T, Olson DG, Tian L, Bomble YJ, Himmel ME, Lo J, Hon S, Shaw
            AJ, van Dijken JP, Lynd LR.
  TITLE     Cofactor Specificity of the Bifunctional Alcohol and Aldehyde
            Dehydrogenase (AdhE) in Wild-Type and Mutant Clostridium
            thermocellum and Thermoanaerobacterium saccharolyticum
  JOURNAL   J. Bacteriol. 197 (15), 2610-2619 (2015)
   PUBMED   26013492
REFERENCE   3  (bases 1 to 10886)
  AUTHORS   Hon S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-DEC-2014) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Drive, Hanover, NH 03755, USA
REFERENCE   4  (bases 1 to 10886)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 10886)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "2015"; volume: "197"; issue: "8"; pages:
            "1386-1393"
            SGRef: number: 2; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "2015"; volume: "197"; issue: "15"; pages:
            "2610-2619"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (05-DEC-2014) Thayer School of Engineering, Dartmouth College, 14
            Engineering Drive, Hanover, NH 03755, USA"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(561..1418)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1419..1523)
                     /label="AmpR promoter"
     misc_feature    1613..2112
                     /label="5' homology flank #1"
                     /note="5' homology flank #1"
     CDS             2691..3338
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             3359..3904
                     /codon_start=1
                     /gene="hpt"
                     /product="hpt"
                     /label="hpt"
                     /note="hypoxanthine phosphoribosyltransferase"
                     /protein_id="AJO16031.1"
                     /translation="MENLSKDIDEILITEEELKEKIKELGRQITKDYKGKNLMLVGVLK
                     GALMFMADLSRHIDLPLSLDFMAVSSYGSSTHSSGIVKIIKDLDISIEGKDVLIVEDII
                     DSGLTLSYLRETLLGRKPKSLKICTILDKPERREASVKVDYVGFKIPDKFVVGYGLDFD
                     EKYRNLPFIGVLKPEMYS"
     gene            3359..3904
                     /gene="hpt"
                     /label="hpt"
     misc_feature    4090..4589
                     /label="5' homology flank #2"
                     /note="5' homology flank #2"
     misc_feature    6952..7451
                     /label="3' homology flank"
                     /note="3' homology flank"
     terminator      7484..7672
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(7932..8520)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8607..9185)
                     /codon_start=1
                     /gene="tdk"
                     /product="Tdk"
                     /label="tdk"
                     /note="thymidine kinase"
                     /protein_id="AJO16032.1"
                     /translation="MYGPKDHGYIEVVTGPMFSGKSEELIRRIKRAKIARQKVQVFKPA
                     IDDRYSIDKVVSHNGDNMHAIAIVKASDILAYAEEDTDVFAIDEVQFFDSEIVDIVKEI
                     ADSGKRVICAGLDMDFRGEPFGPTPELMAIAEFVDKLTAICMKCGNPATRTQRLINGKP
                     ANYDDPIIMVGAKESYEARCRKCHEVPRT"
     gene            complement(8607..9185)
                     /gene="tdk"
                     /label="tdk"
     CDS             complement(9885..10886)
                     /label="repB"
                     /note="RepB replication protein"
ORIGIN
        1 ctatttagtt atttgtttgg tcacttataa ctttaacaga tggggttttt ctgtgcaacc
       61 aattttaagg gttttcaata ctttaaaaca catacatacc aacacttcaa cgcacctttc
      121 agcaactaaa ataaaaatga cgttatttct atatgtatca agaatagaaa gaactcgttt
      181 ttcgctacgc tcaaaacgca aaaaaagcac tcattcgagt gctttttctt atcgctccaa
      241 atcatgcgat tttttcctct ttgcttttct ttgctcacga agttctcgat cacgctgcaa
      301 aacatcttga agcgaaaaag tattcttctt ttcttccgat cgctcatgct gacgcacgaa
      361 aagccctcta ggcgcatagg aacaactcct aaatgcatgt gaggggtttt ctcgtccatg
      421 tgaacagtcg catacgcaat attttgtttc ccatagcggc cgcgattatc aaaaaggatc
      481 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
      541 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
      601 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
      661 cgcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
      721 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
      781 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
      841 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
      901 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
      961 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     1021 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     1081 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     1141 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     1201 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     1261 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     1321 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     1381 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     1441 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     1501 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     1561 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcg atggcttgga
     1621 ggagctcttt tatcctatga gggctttaaa gaatatggat taaatattgt tgcagcgttt
     1681 gatgttgatg aaagtaaaat cggcactgaa atttgcggga gaaaaatttt ccctttggac
     1741 aaaatgaagg aactgtgccg gcggatgaaa attcgtatcg gtataataac cgtccctgca
     1801 gattcagccc aaaaagtatg cgacatgctt gtagacagcg gaatatatgc aatatggaat
     1861 tttgcacctg ttcaccttaa agtaccggat aacattttag tccagcaaga aaacatggcg
     1921 gcatcgctgg ctgtactttc cgcacacttg gcagaagcaa taaagaataa tggaatcgga
     1981 aaaggagata aggaaaatga cgaaaatagc gaataaatac gaagttattg ataatgttga
     2041 aaagcttgaa aaggctttga aacgtttaag agaagctcaa agtgtttatg caacctatac
     2101 acaggagcag gtatcgtggg aataggcatg gaaaagattt ttttgcccga ggatgcattg
     2161 ataaagtatt tcttttccga aagagaggaa aagattctaa agagttttgg aaatactgat
     2221 gaatattgtg tgcagagtac aattctatgg acaagaaaag aggctttgtc aaaacttttt
     2281 cgtctgggaa tgaggatgga ttttaaaaag ctggatactt tggagggcga ggtggttttt
     2341 caggaaacaa acagggcgcg tctgttttct tttatatgca ataattactg tatctctctg
     2401 gcattgccag gttttaataa agattaaaat tattgactag aaataaaaaa attgtccata
     2461 atattaatgg acaaaaaaac aaagaattac atcaaaggaa gataaaaata ctttgttaaa
     2521 aaattaatta ttttttatct aaactattga aaatgaaaat aaaataatat aaaatgaatc
     2581 atagtgcaag agatacttgc cagaggatga atattttact gcattcatgc tttatggcag
     2641 ctaatagagg cattaaatta aattttaatt tacaatagga ggcgatatta atgaacttta
     2701 ataaaattga tttagacaat tggaagagaa aagagatatt taatcattat ttgaaccaac
     2761 aaacgacttt tagtataacc acagaaattg atattagtgt tttataccga aacataaaac
     2821 aagaaggata taaattttac cctgcattta ttttcttagt gacaagggtg ataaactcaa
     2881 atacagcttt tagaactggt tacaatagcg acggagagtt aggttattgg gataagttag
     2941 agccacttta tacaattttt gatggtgtat ctaaaacatt ctctggtatt tggactcctg
     3001 taaagaatga cttcaaagag ttttatgatt tatacctttc tgatgtagag aaatataatg
     3061 gttcggggaa attgtttccc aaaacaccta tacctgaaaa tgctttttct ctttctatta
     3121 ttccatggac ttcatttact gggtttaact taaatatcaa taataatagt aattaccttc
     3181 tacccattat tacagcagga aaattcatta ataaaggtaa ttcaatatat ttaccgctat
     3241 ctttacaggt acatcattct gtttgtgatg gttatcatgc aggattgttt atgaactcta
     3301 ttcaggaatt gtcagatagg cctaatgact ggcttttata ataaaggagg tcgacgtcat
     3361 ggaaaattta tcaaaagaca tcgatgaaat tttgatcaca gaagaagaac ttaaggaaaa
     3421 gataaaagag cttgggaggc aaatcacaaa agactacaaa gggaaaaatt tgatgttggt
     3481 aggagtttta aaaggtgctt taatgtttat ggctgatttg tcaagacaca tagatttgcc
     3541 tttatcactt gattttatgg ctgtttccag ctatggaagc tcaactcatt catcaggaat
     3601 agtaaagata atcaaagatc ttgatataag catagaaggc aaagatgttc tgattgtgga
     3661 agacataatt gacagcggtt tgactttgtc ttacttaagg gaaactttac ttggaaggaa
     3721 gccaaaaagc ctgaaaatat gcacaatatt agacaaaccg gagagaagag aagcatctgt
     3781 aaaagtcgat tatgtaggat ttaagatacc tgataagttt gtcgtgggtt atggattgga
     3841 ctttgatgaa aagtacagga accttccttt tataggcgtt ttgaaacctg aaatgtacag
     3901 ctaagcccgg gcctcgagaa aacaaaaggc tcagtcggaa gactgggcct tttgttttgg
     3961 taccgaattc ggcgcgcctc agcgtttaaa cccgctgatc ctagagggcc gcatcatgta
     4021 attagttatg tcacgcttac attcacgccc tccccatgct ttaatacata cctggcccag
     4081 tagttcagtg gcttggagga gctcttttat cctatgaggg ctttaaagaa tatggattaa
     4141 atattgttgc agcgtttgat gttgatgaaa gtaaaatcgg cactgaaatt tgcgggagaa
     4201 aaattttccc tttggacaaa atgaaggaac tgtgccggcg gatgaaaatt cgtatcggta
     4261 taataaccgt ccctgcagat tcagcccaaa aagtatgcga catgcttgta gacagcggaa
     4321 tatatgcaat atggaatttt gcacctgttc accttaaagt accggataac attttagtcc
     4381 agcaagaaaa catggcggca tcgctggctg tactttccgc acacttggca gaagcaataa
     4441 agaataatgg aatcggaaaa ggagataagg aaaatgacga aaatagcgaa taaatacgaa
     4501 gttattgata atgttgaaaa gcttgaaaag gctttgaaac gtttaagaga agctcaaagt
     4561 gtttatgcaa cctatacaca ggagcaggtt gacaaaattt tctttgaggc ggcaatggcg
     4621 gccaataaaa tgagaattcc tcttgccaaa atggctgtgg aggaaacagg catgggagtg
     4681 gttgaagaca aggttatcaa aaaccactat gcttctgagt atatctataa tgcgtacaaa
     4741 aacactaaaa cctgcggtgt tattgaagag gaccctgctt tcggtattaa aaaaatagca
     4801 gagcctttgg gggttattgc ggcggttata cctactacga atccgacatc gacagcaatc
     4861 tttaagactc ttattgcatt aaagacgaga aatgcaatta ttataagccc acaccccagg
     4921 gcaaaaaact caacgataga agcggcgaaa attgttttgg aggcggccgt taaagccggt
     4981 gctccggaag gcatcattgg ctggattgat gtgccgagcc ttgaacttac caacctggta
     5041 atgagagaag cagatgtgat tctcgcaaca ggcggtcccg gactggttaa agcagcttac
     5101 tcttcgggca aaccggctat tggtgtcgga gcgggcaata ctcctgcaat tattgatgat
     5161 tcggccgaca ttgtcttggc agtgaactca ataatacatt caaaaacttt cgacaacggt
     5221 atgatttgtg cttcagagca atcggtcatt gttctggacg gggtatataa agaggtaaaa
     5281 aaagaatttg aaaaaagagg atgctatttc ttaaatgaag atgaaactga aaaggtaaga
     5341 aaaacaatta taataaacgg tgcgttaaat gccaagatag taggtcagaa agctcacaca
     5401 attgcaaacc ttgcaggttt tgaggtaccc gagactacaa aaattctgat aggcgaagtt
     5461 accagcgtgg atatttccga agaatttgcc cacgaaaagt tgtgcccggt actggcaatg
     5521 tacagggcaa aggattttga cgatgccctt gataaagcag aaaggttggt agctgacggt
     5581 ggatttggcc atacttcgtc actttatata gatacggtaa cacagaaaga gaaacttcag
     5641 aaattctctg aaaggatgaa aacctgccgt atattggtta atacgccgtc atcccaggga
     5701 ggtatcggtg acctttacaa cttcaagctt gctccgtctc tcaccctcgg ctgcggttcc
     5761 tggggaggaa attcagtttc cgacaatgtg ggagtcaagc atttgttaaa cattaaaaca
     5821 gttgccgaga ggagagagaa catgctctgg ttcagaacac ctgaaaagat ttatataaaa
     5881 agaggttgtc tgcctgttgc attggatgag cttaaaaatg taatgggtaa aaagaaagca
     5941 tttattgtaa cggataactt cctgtacaat aacggctaca ccaagccgat tacggataag
     6001 ctggatgaaa tgggaattgt gcacaagacc ttctttgatg tgtctccaga cccatccctt
     6061 gcatctgcca aagccggtgc ggcagaaatg ctggctttcc agcctgacac cataattgcg
     6121 gtcggcggcg gatctgccat ggacgcggcc aaaatcatgt gggtgatgta tgaacatccg
     6181 gaagttgact ttatggacat ggcaatgaga tttatggata taagaaagag agtttacacc
     6241 ttcccgaaga tgggacagaa ggcatacttt atcgcaattc cgacttccgc gggtacaggt
     6301 tcagaagtga caccttttgc ggttattact gatgaaaaaa caggaattaa ataccctctg
     6361 gccgactatg aattgttgcc ggacatggct attgtagatg ccgatatgat gatgaatgct
     6421 ccaaagggac ttaccgcagc ttccggtata gacgcattga cccacgctct ggaagcctat
     6481 gtttcaatgc ttgcgaccga ctatacggat agccttgccc ttcgtgcaat aaagatgata
     6541 tttgaatatc tcccgagagc ctatgaaaac ggtgcaagtg acccggttgc aagagagaaa
     6601 atggccaatg ccgcaacaat agccggaatg gcttttgcca atgccttttt gggtgtatgc
     6661 cattcaatgg cgcacaaact gggtgctttt tatcacctgc cccacggtgt tgccaatgca
     6721 cttatgataa acgaagtaat cagattcaac tcatccgagg ctccgaccaa gatgggtact
     6781 ttcccgcagt atgaccatcc gcgcacgctg gaaaggtatg cagaaattgc cgattatatc
     6841 ggacttaagg gcaagaataa cgaagaaaaa gttgaaaact tgattaaagc tattgatgag
     6901 cttaaagaaa aggtgggcat caggaagacc atcaaagatt atgacataga tgaaaaggaa
     6961 tttttggaca gactggacga aatggtggaa caggcttttg acgaccagtg cacaggtaca
     7021 aatccaagat acccgcttat gaatgaaatc aggcaaatgt atctgaacgc ttattacgga
     7081 ggtgcgaaga aatgaagctt gaaaaggtta tagcggtaag aacggacaaa accgtttaca
     7141 gagacggaga caaggcaata aaggtctttt ccagcaacta ttcaaaggcc aacgtattga
     7201 atgaagcttt aaaccaggcc agggtggaag aaaccgggct taacattccg aagcttcttg
     7261 aagttacaaa aattgatggc aaatgggcaa ttgtgacgga gtttattgaa ggaaagacct
     7321 tagagcagct tatgaaagaa aatcccttaa aatatgatga atatatgaac ctctttgttg
     7381 atattcagct cgaagtgtta agcaaaaggg ctcctttatt aaacaagctg aaagacaaaa
     7441 tgcacagaaa actgctaata gtagtgaaaa aatcagggat aatgccgctc taaccgaaaa
     7501 ggaaggagtt agacaacctg aagtctaggt ccctatttat ttttttatag ttatgttagt
     7561 attaagaacg ttatttatat ttcaaatttt tctttttttt ctgtacagac gcgtgtacgc
     7621 atgtaacatt atactgaaaa ccttgcttga gaaggttttg ggacgctcga aggctttaat
     7681 ttgcaagctg cggccctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7741 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7801 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7861 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7921 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7981 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8041 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8101 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8161 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8221 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8281 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8341 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     8401 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8461 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8521 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8581 gggattttgg tcatgaaaaa atccgcttaa gtccgcggga cttcatggca ctttctacac
     8641 cttgcttcat aagactcctt tgctccaacc attataatgg gatcgtcgta attggcaggc
     8701 ttcccattta taagcctttg cgtgcgagta gcaggattgc cacacttcat gcatatagca
     8761 gtaagcttat cgacaaattc agctatggcc atcaattctg gagtcggacc aaatggttca
     8821 cctctaaagt ccatgtcaag ccctgcacat atgactcttt tcccgctatc ggcaatctct
     8881 tttacgatgt cgactatttc agaatcaaaa aattgaactt catctatagc aaatacatcc
     8941 gtatcttctt cagcataagc caatatgtca gaagccttta ctatggcaat ggcgtgcatg
     9001 ttgtcgccgt tatgagatac gactttgtct atggaatacc tatcgtctat agccggtttg
     9061 aaaacctgaa ctttttgcct ggcaatctta gctctcttta tccttcttat aagttcctca
     9121 ctttttccac tgaacatggg accagttaca acttctatgt acccgtggtc tttaggccca
     9181 tacatctttc gtcctcctta aaattttcgt ttatttgttt tttatttagt gtgcctaaaa
     9241 gcaccatctt atacattcgt accttatatc tcttatcttg tatctcttac ctcatatctc
     9301 ttattattaa ctaccgaagt atattttcat aacggaatct tatgacttat ttttctgact
     9361 gccgttttac catacggtta atttatacgg aaacagctaa tctaaccttg ctgaaggcat
     9421 gtttaccaaa atattatatc tactttacca tattaattca agtggtcatt ttgatatttt
     9481 acgccaaaat ttttagcgtt cctttacttg ataatttatc caaaaacctt cattcgtcaa
     9541 aacaattatg atataattaa tttatgatat gattaattaa tatttacgga taactaatca
     9601 tttcaatgca atagatcaac atgcaaatca atatccacct tactccgaac attttgcacc
     9661 aacgaatgcc cagcactata tcccggcaag caatataacg tctataattt tttatgataa
     9721 cgataaaatt aaattttatg aaaggggtaa aagcatgaga aaaatagctg tattgttaat
     9781 tactttgacg ttcttagtca cgactcgaat ttaggaggct tacttgtctg ctttcttcat
     9841 tagaatcaat ccttttttaa aagtcaatcc cgtttgttga actactcttt aataaaataa
     9901 tttttccgtt cccaattcca cattgcaata atagaaaatc catcttcatc ggctttttcg
     9961 tcatcatctg tatgaatcaa atcgccttct tctgtgtcat caaggtttaa ttttttatgt
    10021 atttctttta acaaaccacc ataggagatt aaccttttac ggtgtaaacc ttcctccaaa
    10081 tcagacaaac gtttcaaatt cttttcttca tcatcggtca taaaatccgt atcctttaca
    10141 ggatattttg cagtttcgtc aattgccgat tgtatatccg atttatattt atttttcggt
    10201 cgaatcattt gaacttttac atttggatca tagtctaatt tcattgcctt tttccaaaat
    10261 tgaatccatt gtttttgatt cacgtagttt tctgtattct taaaataagt tggttccaca
    10321 cataccaata catgcatgtg ctgattataa gaattatctt tattatttat tgtcacttcc
    10381 gttgcacgca taaaaccaac aagattttta ttaatttttt tatattgcat cattcggcga
    10441 aatccttgag ccatatctga caaactctta tttaattctt cgccatcata aacattttta
    10501 actgttaatg tgagaaacaa ccaacgaact gttggctttt gtttaataac ttcagcaaca
    10561 accttttgtg actgaatgcc atgtttcatt gctctcctcc agttgcacat tggacaaagc
    10621 ctggatttac aaaaccacac tcgatacaac tttctttcgc ctgtttcacg attttgttta
    10681 tactctaata tttcagcaca atcttttact ctttcagcct ttttaaattc aagaatatgc
    10741 agaagttcaa agtaatcaac attagcgatt ttcttttctc tccatggtct cacttttcca
    10801 ctttttgtct tgtccactaa aacccttgat ttttcatctg aataaatgct actattagga
    10861 cacataatat taaaagaaac ccccat
//
