LOCUS       62056_19670        5805 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5805
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        74..92
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             128..1060
                     /codon_start=1
                     /label=hRluc
                     /note="Renilla luciferase"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             1267..2916
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="IEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(2963..3084)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3503..4091)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4294..5151)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5256..5304
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    5318..5409
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     promoter        5515..5805
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
       61 ttgcaaaaag ctctaatacg actcactata gggagcttgg caatccggta ctgttggtaa
      121 agccaccatg gcttccaagg tgtacgaccc cgagcaacgc aaacgcatga tcactgggcc
      181 tcagtggtgg gctcgctgca agcaaatgaa cgtgctggac tccttcatca actactatga
      241 ttccgagaag cacgccgaga acgccgtgat ttttctgcat ggtaacgctg cctccagcta
      301 cctgtggagg cacgtcgtgc ctcacatcga gcccgtggct agatgcatca tccctgatct
      361 gatcggaatg ggcaaatccg gcaagagcgg gaatggctca tatcgcctcc tggatcacta
      421 caagtacctc accgcttggt tcgagctgct gaaccttcca aagaaaatca tctttgtggg
      481 ccacgactgg ggggcttgtc tggcctttca ctactcctac gagcaccaag acaagatcaa
      541 ggccatcgtc catgctgaga gtgtcgtgga cgtgatcgag tcctgggacg agtggcctga
      601 catcgaggag gatatcgccc tgatcaagag cgaagagggc gagaaaatgg tgcttgagaa
      661 taacttcttc gtcgagacca tgctcccaag caagatcatg cggaaactgg agcctgagga
      721 gttcgctgcc tacctggagc cattcaagga gaagggcgag gttagacggc ctaccctctc
      781 ctggcctcgc gagatccctc tcgttaaggg aggcaagccc gacgtcgtcc agattgtccg
      841 caactacaac gcctaccttc gggccagcga cgatctgcct aagatgttca tcgagtccga
      901 ccctgggttc ttttccaacg ctattgtcga gggagctaag aagttcccta acaccgagtt
      961 cgtgaaggtg aagggcctcc acttcagcca ggaggacgct ccagatgaaa tgggaaaata
     1021 catcaagagc ttcgtggagc gcgtgctgaa gaacgagcag tatttctaca cctcgagaga
     1081 agccagacac aaacagaaaa ttgtggcacc gtactcgagc agaagcttag atctgaggca
     1141 cggcataagc aaaagatcga ttttaaacgg gtttgcggtg taagtgcagc ccgtcttaca
     1201 ccgtgcggca caggcactag tactgatgtc gtatacaggg cttttgatcc attcggatct
     1261 gccaccattg aagatgccaa aaacattaag aagggcccag cgccattcta cccactcgaa
     1321 gacgggaccg ccggcgagca gctgcacaaa gccatgaagc gctacgccct ggtgcccggc
     1381 accatcgcct ttaccgacgc acatatcgag gtggacatta cctacgccga gtacttcgag
     1441 atgagcgttc ggctggcaga agctatgaag cgctatgggc tgaatacaaa ccatcggatc
     1501 gtggtgtgca gcgagaatag cttgcagttc ttcatgcccg tgttgggtgc cctgttcatc
     1561 ggtgtggctg tggccccagc taacgacatc tacaacgagc gcgagctgct gaacagcatg
     1621 ggcatcagcc agcccaccgt cgtattcgtg agcaagaaag ggctgcaaaa gatcctcaac
     1681 gtgcaaaaga agctaccgat catacaaaag atcatcatca tggatagcaa gaccgactac
     1741 cagggcttcc aaagcatgta caccttcgtg acttcccatt tgccacccgg cttcaacgag
     1801 tacgacttcg tgcccgagag cttcgaccgg gacaaaacca tcgccctgat catgaacagt
     1861 agtggcagta ccggattgcc caagggcgta gccctaccgc accgcaccgc ttgtgtccga
     1921 ttcagtcatg cccgcgaccc catcttcggc aaccagatca tccccgacac cgctatcctc
     1981 agcgtggtgc catttcacca cggcttcggc atgttcacca cgctgggcta cttgatctgc
     2041 ggctttcggg tcgtgctcat gtaccgcttc gaggaggagc tattcttgcg cagcttgcaa
     2101 gactataaga ttcaatctgc cctgctggtg cccacactat ttagcttctt cgctaagagc
     2161 actctcatcg acaagtacga cctaagcaac ttgcacgaga tcgccagcgg cggggcgccg
     2221 ctcagcaagg aggtaggtga ggccgtggcc aaacgcttcc acctaccagg catccgccag
     2281 ggctacggcc tgacagaaac aaccagcgcc attctgatca cccccgaagg ggacgacaag
     2341 cctggcgcag taggcaaggt ggtgcccttc ttcgaggcta aggtggtgga cttggacacc
     2401 ggtaagacac tgggtgtgaa ccagcgcggc gagctgtgcg tccgtggccc catgatcatg
     2461 agcggctacg ttaacaaccc cgaggctaca aacgctctca tcgacaagga cggctggctg
     2521 cacagcggcg acatcgccta ctgggacgag gacgagcact tcttcatcgt ggaccggctg
     2581 aagagcctga tcaaatacaa gggctaccag gtagccccag ccgaactgga gagcatcctg
     2641 ctgcaacacc ccaacatctt cgacgccggg gtcgccggcc tgcccgacga cgatgccggc
     2701 gagctgcccg ccgcagtcgt cgtgctggaa cacggtaaaa ccatgaccga gaaggagatc
     2761 gtggactatg tggccagcca ggttacaacc gccaagaagc tgcgcggtgg tgttgtgttc
     2821 gtggacgagg tgcctaaagg actgaccggc aagttggacg cccgcaagat ccgcgagatt
     2881 ctcattaagg ccaagaaggg cggcaagatc gccgtgtaat aattctagag tcggggcggc
     2941 cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga
     3001 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     3061 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     3121 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc
     3181 gataaggatc cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg
     3241 ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt
     3301 aggacaggtg ccggcagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     3361 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     3421 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     3481 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     3541 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3601 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3661 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3721 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3781 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3841 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3901 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     3961 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     4021 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     4081 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     4141 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     4201 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     4261 gcggccgcaa atgctaaacc actgcagtgg ttaccagtgc ttgatcagtg aggcaccgat
     4321 ctcagcgatc tgcctatttc gttcgtccat agtggcctga ctccccgtcg tgtagatcac
     4381 tacgattcgt gagggcttac catcaggccc cagcgcagca atgatgccgc gagagccgcg
     4441 ttcaccggcc cccgatttgt cagcaatgaa ccagccagca gggagggccg agcgaagaag
     4501 tggtcctgct actttgtccg cctccatcca gtctatgagc tgctgtcgtg atgctagagt
     4561 aagaagttcg ccagtgagta gtttccgaag agttgtggcc attgctactg gcatcgtggt
     4621 atcacgctcg tcgttcggta tggcttcgtt caactctggt tcccagcggt caagccgggt
     4681 cacatgatca cccatattat gaagaaatgc agtcagctcc ttagggcctc cgatcgttgt
     4741 cagaagtaag ttggccgcgg tgttgtcgct catggtaatg gcagcactac acaattctct
     4801 taccgtcatg ccatccgtaa gatgcttttc cgtgaccggc gagtactcaa ccaagtcgtt
     4861 ttgtgagtag tgtatacggc gaccaagctg ctcttgcccg gcgtctatac gggacaacac
     4921 cgcgccacat agcagtactt tgaaagtgct catcatcggg aatcgttctt cggggcggaa
     4981 agactcaagg atcttgccgc tattgagatc cagttcgata tagcccactc ttgcacccag
     5041 ttgatcttca gcatctttta ctttcaccag cgtttcgggg tgtgcaaaaa caggcaagca
     5101 aaatgccgca aagaagggaa tgagtgcgac acgaaaatgt tggatgctca tactcgtcct
     5161 ttttcaatat tattgaagca tttatcaggg ttactagtac gtctctcaag gataagtaag
     5221 taatattaag gtacgggagg tattggacag gccgcaataa aatatcttta ttttcattac
     5281 atctgtgtgt tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca
     5341 aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa
     5401 catttctctg gcctaactgg ccggtacctg agctcgctag cttcgaggat atcagatcag
     5461 cgcagcacca tggcctgaaa taacctctga aagaggaact tggttaggta ccttctgagg
     5521 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     5581 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     5641 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     5701 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     5761 gccccatggc tgactaattt tttttattta tgcagaggcc gaggc
//
