LOCUS       40924_39877        7108 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pSG58, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7108)
  AUTHORS   Goldfless SJ, Wagner JC, Niles JC.
  TITLE     Versatile control of Plasmodium falciparum gene expression with an 
            inducible protein-RNA interaction
  JOURNAL   Nat Commun 5, 5329 (2014)
  PUBMED    25370483
REFERENCE   2  (bases 1 to 7108)
  AUTHORS   Goldfless SJ, Niles JC.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-AUG-2014) Biological Engineering, MIT, 77 
            Massachusetts Avenue, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 7108)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7108)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun 
            5, 5329 (2014)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-AUG-2014) Biological Engineering, MIT, 77 Massachusetts Avenue, 
            Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(7..25)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(43..59)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(67..83)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(91..121)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(136..157)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(445..1033)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1207..2064)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2065..2169)
                     /label=AmpR promoter
     primer_bind     2643..2659
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2666..2683
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_recomb     2689..2738
                     /label=Bxb1 attP
                     /note="Bxb1 attP"
     misc_recomb     2745..2795
                     /label=Bxb1 attP
                     /note="Bxb1 attP"
     regulatory      complement(2809..3405)
                     /label=derived from P. falciparum HRPII 3' UTR
                     /note="derived from P. falciparum HRPII 3' UTR"
                     /regulatory_class="terminator"
     CDS             complement(3414..3806)
                     /label=BSD
                     /note="blasticidin S deaminase"
     regulatory      complement(3824..4418)
                     /note="contains promoter and 5' UTR from P. chabaudi 
                     dihydrofolate reductase-thymidylate synthase gene"
                     /regulatory_class="promoter"
     regulatory      4501..5512
                     /note="contains promoter and 5' UTR from P. falciparum 
                     calmodulin gene"
                     /regulatory_class="promoter"
     CDS             5517..5549
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             5565..6185
                     /label=TetR
                     /note="tetracycline repressor TetR"
     misc_feature    6186..6206
                     /label=Region: linker
                     /note="Region: linker"
     CDS             6207..6224
                     /label=6xHis
                     /note="6xHis affinity tag"
     regulatory      6233..7069
                     /label=derived from P. falciparum hsp86 3' UTR
                     /note="derived from P. falciparum hsp86 3' UTR"
                     /regulatory_class="terminator"
ORIGIN
        1 gagtattcta tagtgtcacc taaatagctt ggcgtaatca tggtcatagc tgtttcctgt
       61 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
      121 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
      181 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
      241 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
      301 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
      361 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
      421 taaaaaggcc gcgttgctgg cgtttttcga taggctccgc ccccctgacg agcatcacaa
      481 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      541 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
      601 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
      661 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
      721 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
      781 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
      841 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
      901 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
      961 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1021 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     1081 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     1141 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     1201 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     1261 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     1321 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     1381 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     1441 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     1501 caacgttgtt ggcattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     1561 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     1621 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     1681 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     1741 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     1801 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     1861 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     1921 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     1981 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     2041 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     2101 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     2161 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     2221 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
     2281 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     2341 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     2401 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     2461 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
     2521 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     2581 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     2641 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagaattctg gtttgtctgg
     2701 tcaaccaccg cggtctcagt ggtgtacggt acaaacccga attctggttt gtctggtcaa
     2761 ccaccgcggt ctcagtggtg tacggtacaa acccggaatt cgagctcggt acctctagat
     2821 ttctctgcgg tttaataaat atgtccttat atataatgag aaataaatat ttaacatatg
     2881 tttaaaaaga aaaatttaag atttacatga ttaggaataa aaatattaaa agctatatat
     2941 taatgttgta ctttatgttc atattgtaaa agataaatat aatttttatt aaaatttata
     3001 tttattatta ttataagttc ttagaaaaaa cattttgttt taaaataata ctataaaaag
     3061 aacaataata aaaataaaat acaattaaaa cataattaaa aaagaaaaat acagacagta
     3121 aaaaaaatcg ctatcccata aattacaaaa catgaattaa taaacatata ttgtttatta
     3181 ataaaattgt aagttatttt tatatataca taataaatta aagatactga aacaataatt
     3241 ttaaatgtaa ataaatgaaa aatgatatgt tttatatgat tcattattct atatttataa
     3301 ggaagattac aaaaaaaaaa ttcatatgta ttttttttgt aatttctgtg tttatgtttt
     3361 tatttttata atatttttaa tctattatta aataaattta atggggtacc ttagccctcc
     3421 cacacataac cagagggcag caattcacga atcccaactg ccgtcggctg tccatcactg
     3481 tccttcacta tggctttgat cccaggatgc agatcgagaa gcacctgtcg gcaccgtccg
     3541 caggggctca agatgcccct gttctcattt ccgatcgcga cgatacaagt caggttgcca
     3601 gctgccgcag cagcagcagt gcccagcacc acgagttctg cacaaggtcc cccagtaaaa
     3661 tgatatacat tgacaccagt gaagatgcgg ccgtcgctag agagagctgc gctggcgacg
     3721 ctgtagtctt cagagatggg gatgctgttg attgtagccg ttgctctttc aatgagggtg
     3781 gattcttctt gagacaaagg cttggcatgc atttatcacc atggtttgta agttttaggt
     3841 gtgtgtatat atataagcac tacacaattg ttactagcaa atgtagaaag aaatagccag
     3901 tatttaaact aaatatgtga atattaaaat atagtgacaa aaaaggaaag tgtatgtaaa
     3961 ataaaatgaa aattgaaaag aatttatatt ataatataaa aaattgtagt gaacatatat
     4021 aatcctgtgt tatatatatt taatattatt attgactttt ttaaaaaaaa tatttattat
     4081 aaaaattttt taatttatgc acttttttat tgtacttata tatattccat tattttactt
     4141 acacctaaat ttagtagaaa attttattca acccaaataa aaattatggc gctatatagg
     4201 atgagatcat atattttttt ctctttttca aatcctttat attttatcat acaaactgtt
     4261 ttaaacacga gaaaaagggg atgccatgca catgcttagt acacatgcaa tagatatata
     4321 tagtatatat attacatata tatatgtgta cttgttaaaa tgctatatca aatagttttg
     4381 cataaacgag agtacctctt acaagcgaat tagctaagca tgcgggccct ataaggaaat
     4441 tcccatatat aaaaaaaaaa agtacctatt aataattata tggatcgaag cttgcatgcc
     4501 tctgagcttc ttctttgtta accattttgt aaaaaaaatt aaaatatatt tatataatat
     4561 tattttattt tattatatat tatattattt ttatttttat ttttattttt ttttctctac
     4621 aaattttatc tattggttta ttataaaaat atctatttct aataataaat aattaagata
     4681 tcaatttata gaaacaaaat atatacttgt ataattttat ttttttatat aaatcattac
     4741 atatataatt atacaatatt ttttctaaga gataattata tattaatata tataaaaaaa
     4801 ggtgtttttt tttttttttt ttatttttat ttttatttta tggtaatatt ttattttcct
     4861 tattttataa attatattag tttatatgtg attaatttta tatattatca atttatatat
     4921 ttttaaatgc ttacttaatt atcttttttt tttttttttt ttttttttcc cctcttttta
     4981 tattaattta tttttgaaaa aattgatata tatatatata tataatatat atatatacat
     5041 gtagtagtat taaacaatgt ataatatata taaataatat atttatatat ttcatttcaa
     5101 ttttaatttt ttttggtttt tttttttttt ctttttgtca tatttaaaaa aaattatatt
     5161 catataagtt atgcattttt tataaacatt attcaatata tgtataatat aatatatata
     5221 tatatattaa tgtattattc caatgtgcat gataaaagaa aaaaataata tttataaaaa
     5281 aaaagaaaaa taaaacaaaa aaagaaaaaa aaaaaaaaaa aaaaaaaaat acaaaaataa
     5341 ataatataat ttataattat atattcttgt cacaataaaa atatatatat atatatatat
     5401 atttataata tgtatatttt aaactagaaa aggaataact aatattttat ttattatcat
     5461 tcaagattta tattttataa taataaatac ctaatagaaa tatatcacct aggaccatgg
     5521 ctagcatgac tggtggacag caaatgggtc gcggatccga attcatgtct agattagata
     5581 aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa
     5641 caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa
     5701 aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc
     5761 acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt
     5821 ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta
     5881 cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt
     5941 cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat
     6001 tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta
     6061 tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct
     6121 tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg
     6181 ggtctaagct tgcggccgca ctcgagcacc accaccacca ccactaactt aaggtcgagt
     6241 tatataatat atttatgtac tcacaatggg gtctacaaaa aaaaaaaaat aaagatatat
     6301 atatatatat attatctttc attttatcat caccccaata tacatatata tatttttata
     6361 tttttatata tttatatttt tcttttattt atccataaaa gaaaattaaa tataaattta
     6421 taccttttat ttgaattacc atttgtataa aatataaaaa aaaaaaaaaa taataaaaag
     6481 aagaaaaaac gaaaaaagta atatttaaat aataaagatc aatatacata ataaaaaaaa
     6541 aaaaaaaaaa aaaaattaaa atttaaaaaa ttaaacattg aaataatttc atatacacac
     6601 atatacatac atacatatat atatatatat atatatattt atacatttat gcatataaaa
     6661 tatattaaca acttatgtgt attcatatat atttttttac atatgtattg aaagacttta
     6721 ctgagacatg aaatatctaa taaccaaacg tttataaatt ctaattatat gtaaagcata
     6781 atatagcttt tgtctaattt aattccttaa aacgtatgta ttaaaaatat agtaagatgg
     6841 ggaaattata attttttttt taaaggtttt tctttgatac agtaaaaaaa aaaaataaaa
     6901 aaaaataata ataataaaaa aataaaaaaa taaaaaaaaa taaaataatt ataaagatat
     6961 atataattcc tcccaaatac aaatgattaa gtttaaaaat taataaataa atcaaaaaaa
     7021 aatttaataa taattgtatt attttaagtg tagttaattc atcaaatagc atgcctgcag
     7081 gtcgactcta gaggatcccc ccaagctt
//
