LOCUS       62056_19665        4076 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4076
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..366
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     intron          395..967
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     promoter        1022..1040
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    1072..1193
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1398..1986)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2160..3017)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3018..3122)
                     /label=AmpR promoter
     rep_origin      complement(3455..3910)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     4055..4071
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgacttct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
       61 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
      121 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
      181 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
      241 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
      301 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
      361 tgcaaaaagc tggatcgatc ctgagaactt cagggtgagt ttggggaccc ttgattgttc
      421 tttctttttc gctattgtaa aattcatgtt atatggaggg ggcaaagttt tcagggtgtt
      481 gtttagaatg ggaagatgtc ccttgtatca ccatggaccc tcatgataat tttgtttctt
      541 tcactttcta ctctgttgac aaccattgtc tcctcttatt ttcttttcat tttctgtaac
      601 tttttcgtta aactttagct tgcatttgta acgaattttt aaattcactt ttgtttattt
      661 gtcagattgt aagtactttc tctaatcact tttttttcaa ggcaatcagg gtatattata
      721 ttgtacttca gcacagtttt agagaacaat tgttataatt aaatgataag gtagaatatt
      781 tctgcatata aattctggct ggcgtggaaa tattcttatt ggtagaaaca actacatcct
      841 ggtcatcatc ctgcctttct ctttatggtt acaatgatat acactgtttg agatgaggat
      901 aaaatactct gagtccaaac cgggcccctc tgctaaccat gttcatgcct tcttcttttt
      961 cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt
     1021 gtaatacgac tcactatagg gcgaattcgg atccagatct tattaaagca gaacttgttt
     1081 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     1141 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     1201 tggtcgactc tagactcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1261 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1321 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1381 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1441 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1501 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1561 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
     1621 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1681 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1741 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1801 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     1861 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1921 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     1981 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2041 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     2101 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     2161 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     2221 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     2281 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     2341 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     2401 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     2461 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     2521 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     2581 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     2641 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     2701 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     2761 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     2821 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     2881 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     2941 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     3001 aaatgttgaa tactcatact cttctttttt caatattatt gaagcattta tcagggttat
     3061 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     3121 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     3181 acctataaaa ataggcgtat cacgaggccc ctttcgtctc gcgcgtttcg gtgatgacgg
     3241 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     3301 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     3361 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     3421 gcacagatgc gtaaggagaa aataccgcat caggaaattg taaacgttaa tattttgtta
     3481 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     3541 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     3601 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     3661 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     3721 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     3781 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     3841 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     3901 cagggcgcgt cgcgccattc gccattcagg ctacgcaact gttgggaagg gcgatcggtg
     3961 cgggcctctt cgctattacg ccagctggcg aaggggggat gtgctgcaag gcgattaagt
     4021 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaatt
//
