LOCUS       40924_39862       11625 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pSG252, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11625)
  AUTHORS   Goldfless SJ, Wagner JC, Niles JC.
  TITLE     Versatile control of Plasmodium falciparum gene expression with an 
            inducible protein-RNA interaction
  JOURNAL   Nat Commun 5, 5329 (2014)
  PUBMED    25370483
REFERENCE   2  (bases 1 to 11625)
  AUTHORS   Goldfless SJ, Niles JC.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-AUG-2014) Biological Engineering, MIT, 77 
            Massachusetts Avenue, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 11625)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11625)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun 
            5, 5329 (2014)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-AUG-2014) Biological Engineering, MIT, 77 Massachusetts Avenue, 
            Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11625
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      complement(24..859)
                     /label=derived from P. falciparum hsp86 3' UTR
                     /note="derived from P. falciparum hsp86 3' UTR"
                     /regulatory_class="terminator"
     CDS             complement(869..2518)
                     /label=luciferase
                     /note="firefly luciferase"
     misc_feature    complement(2541..2587)
                     /label=mutant TetR aptamer
                     /note="mutant TetR aptamer"
     regulatory      complement(2590..3219)
                     /note="contains promoter and 5' UTR from P. falciparum 
                     calmodulin gene"
                     /regulatory_class="promoter"
     regulatory      3220..4089
                     /note="contains promoter and 5'UTR from P. falciparum Hsp86
                     (PF07_0029)"
                     /regulatory_class="promoter"
     CDS             4100..4720
                     /label=TetR
                     /note="tetracycline repressor TetR"
     CDS             4730..4783
                     /label=T2A
                     /note="2A peptide from Thosea asigna virus capsid protein"
     CDS             4784..5719
                     /label=RLuc
                     /note="Renilla luciferase"
     misc_feature    5717..5779
                     /note="Thosea asigna virus 2A-like peptide, P. falciparum 
                     optimized codon usage"
     CDS             5726..5779
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5780..6568
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      6607..7170
                     /label=derived from P. falciparum HRPII 3' UTR
                     /note="derived from P. falciparum HRPII 3' UTR"
                     /regulatory_class="terminator"
     gene            complement(7218..8574)
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     repeat_region   8582..9120
     repeat_region   9121..9675
     misc_feature    complement(9829..10332)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     rep_origin      10583..11171
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    11459..11480
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        11495..11525
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11533..11549
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11557..11573
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11591..11609
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 ggatcctcta gagtcgacct gcaggcatgc tatttgatga attaactaca cttaaaataa
       61 tacaattatt attaaatttt tttttgattt atttattaat ttttaaactt aatcatttgt
      121 atttgggagg aattatatat atctttataa ttattttatt tttttttatt tttttatttt
      181 tttattatta ttattttttt ttattttttt tttttactgt atcaaagaaa aacctttaaa
      241 aaaaaaatta taatttcccc atcttactat atttttaata catacgtttt aaggaattaa
      301 attagacaaa agctatatta tgctttacat ataattagaa tttataaacg tttggttatt
      361 agatatttca tgtctcagta aagtctttca atacatatgt aaaaaaatat atatgaatac
      421 acataagttg ttaatatatt ttatatgcat aaatgtataa atatatatat atatatatat
      481 atatgtatgt atgtatatgt gtgtatatga aattatttca atgtttaatt ttttaaattt
      541 taattttttt tttttttttt ttttttatta tgtatattga tctttattat ttaaatatta
      601 cttttttcgt tttttcttct ttttattatt tttttttttt tttatatttt atacaaatgg
      661 taattcaaat aaaaggtata aatttatatt taattttctt ttatggataa ataaaagaaa
      721 aatataaata tataaaaata taaaaatata tatatgtata ttggggtgat gataaaatga
      781 aagataatat atatatatat atatctttat tttttttttt ttgtagaccc cattgtgagt
      841 acataaatat attatataac tcgagttaca actcggactt tccgcccttc ttggccttta
      901 tgaggatctc tctgattttt cttgcgtcga gttttccggt aagacctttc ggtacttcgt
      961 ccacaaacac aactcctccg cgcaactttt tcgcggttgt tacttgactg gcgacgtaat
     1021 ccacgatctc tttttccgtc atcgtctttc cgtgctccaa aacaacaacg gcggcgggaa
     1081 gttcaccggc gtcatcgtcg ggaagacctg ccacgcccgc gtcgaagatg ttggggtgtt
     1141 gtaacaatat cgattccaat tcagcggggg ccacctgata tcctttgtat ttaattaaag
     1201 acttcaagcg gtcaactatg aagaagtgtt cgtcttcgtc ccagtaagct atgtctccag
     1261 aatgtagcca tccatccttg tcaatcaagg cgttggtcgc ttccggattg tttacataac
     1321 cggacataat cataggtcct ctgacacata attcgcctct ctgattaacg cccagcgttt
     1381 tcccggtatc cagatccaca accttcgctt caaaaaatgg aacaacttta ccgaccgcgc
     1441 ccggtttatc atccccctcg ggtgtaatca gaatagctga tgtagtctca gtgagcccat
     1501 atccttgtcg tatccctgga agatggaagc gttttgcaac cgcttccccg acttctttcg
     1561 aaagaggtgc gcccccagaa gcaatttcgt gtaaattaga taaatcgtat ttgtcaatca
     1621 gagtgctttt ggcgaagaat gaaaataggg ttggtactag caacgcactt tgaattttgt
     1681 aatcctgaag ggatcgtaaa aacagctctt cttcaaatct atacattaag acgactcgaa
     1741 atccacatat caaatatccg agtgtagtaa acattccaaa accgtgatgg aatggaacaa
     1801 cacttaaaat cgcagtatcc ggaatgattt gattgccaaa aataggatct ctggcatgcg
     1861 agaatctgac gcaggcagtt ctatgcggaa gggccacacc cttaggtaac ccagtagatc
     1921 cagaggaatt cattatcagt gcaattgttt tgtcacgatc aaaggactct ggtacaaaat
     1981 cgtattcatt aaaaccggga ggtagatgag atgtgacgaa cgtgtacatc gactgaaatc
     2041 cctggtaatc cgttttagaa tccatgataa taattttctg gattattggt aatttttttt
     2101 gcacgttcaa aattttttgc aacccctttt tggaaacaaa cactacggta ggctgcgaaa
     2161 tgttcatact gttgagcaat tcacgttcat tataaatgtc gttcgcgggc gcaactgcaa
     2221 ctccgataaa taacgcgccc aacaccggca taaagaattg aagagagttt tcactgcata
     2281 cgacgattct gtgatttgta ttcagcccat atcgtttcat agcttctgcc aaccgaacgg
     2341 acatttcgaa gtattccgcg tacgtgatgt tcacctcgat atgtgcatct gtaaaagcaa
     2401 ttgttccagg aaccagggcg tatctcttca tagccttatg cagttgctct ccagcggttc
     2461 catcctctag tggatagaat ggcgccgggc ctttctttat gtttttggcg tcttccatat
     2521 gttctcgagt tttgtgtttt atccaggcca tcacattccg tccgtatcga ccttacactg
     2581 cctggattga tatatttcta ttaggtattt attattataa aatataaatc ttgaatgata
     2641 ataaataaaa tattagttat tccttttcta gtttaaaata tacatattat aaatatatat
     2701 atatatatat atatttttat tgtgacaaga atatataatt ataaattata ttatttattt
     2761 ttgtattttt tttttttttt tttttttttt ctttttttgt tttatttttc ttttttttta
     2821 taaatattat ttttttcttt tatcatgcac attggaataa tacattaata tatatatata
     2881 tattatatta tacatatatt gaataatgtt tataaaaaat gcataactta tatgaatata
     2941 atttttttta aatatgacaa aaagaaaaaa aaaaaaaacc aaaaaaaatt aaaattgaaa
     3001 tgaaatatat aaatatatta tttatatata ttatacattg tttaatacta ctacatgtat
     3061 atatatatat tatatatata tatatatatc aattttttca aaaataaatt aatataaaaa
     3121 gaggggaaaa aaaaaaaaaa aaaaaaaaaa agataattaa gtaagcattt aaaaatatat
     3181 aaattgataa tatataaaat taatcacata taaaagcttc ggaaaggggc cattggatat
     3241 atatttagta ttccatagta tatagttaaa tataataata ttatagtaaa tttttgcatg
     3301 aatttaaaga aaaaaaaaaa aaaaataaca aaaaggagta ttatataaat ataacaaaat
     3361 aataaatact atacctatat atatatatat atatatatac atatatataa atatataaat
     3421 atataaatat atttatataa aaataatatt ttatatataa tattatattt ttataatatc
     3481 tttttttttt taaatatcat ctttgtttct ttctttcttt ctttctttct ttcttttttt
     3541 ttttttttga tttatatatt attttatttt gtatttacaa aatataataa aataatttca
     3601 tgtttagcaa aaattttaaa taaaaaataa taatataaaa taaaaagcat catttaatat
     3661 aaaataataa aattaaaata aaataagata attaccaaaa ataataaaaa aaattaaaaa
     3721 gtattaaaaa atatatatca tatataaata tataaatttt aacaacgtga gaaattaaga
     3781 aaaaaattat atttatataa ataatataat atttaatata tatctcatat aatattatat
     3841 atatatatat atatatatat atatttttta aaaaaaaaga aaaaataagt ttatttttac
     3901 cttttaataa aaacatattt ttagatatac ataaaaattt ttatattatt ttattggtat
     3961 atatatatat atatatatat ataataatat atatataata aaatatataa atatttacat
     4021 aaatatttta ctttaaaaag aaattttcct tttttattat attttttttc ttcccacatt
     4081 tcgaataaac ctaggaaaaa tgtctagatt agataaaagt aaagtgatta acagcgcatt
     4141 agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct
     4201 tggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc
     4261 cttagccatt gagatgttag ataggcacca tactcacttc tgccctttag aaggggaaag
     4321 ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg
     4381 cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga
     4441 aaatcaatta gcctttttat gccaacaagg tttttcacta gagaacgcgt tatatgcact
     4501 cagcgctgtg gggcatttta ccttaggttg cgtattggaa gatcaagagc atcaagtcgc
     4561 taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac gacaagctat
     4621 cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat
     4681 atgcggatta gaaaaacaac ttaaatgtga aagtgggtct ggtagtggtg aaggtagagg
     4741 atcattatta acatgtggtg atgttgaaga aaatccaggt ccaatgactt cgaaagttta
     4801 tgatccagaa caaaggaaac ggatgataac tggtccgcag tggtgggcca gatgtaaaca
     4861 aatgaatgtt cttgattcat ttattaatta ttatgattca gaaaaacatg cagaaaatgc
     4921 tgttattttt ttacatggta acgcggcctc ttcttattta tggcgacatg ttgtgccaca
     4981 tattgagcca gtagcgcggt gtattatacc agaccttatt ggtatgggca aatcaggcaa
     5041 atctggtaat ggttcttata ggttacttga tcattacaaa tatcttactg catggtttga
     5101 acttcttaat ttaccaaaga agatcatttt tgtcggccat gattggggtg cttgtttggc
     5161 atttcattat agctatgagc atcaagataa gatcaaagca atagttcacg ctgaaagtgt
     5221 agtagatgtg attgaatcat gggatgaatg gcctgatatt gaagaagata ttgcgttgat
     5281 caaatctgaa gaaggagaaa aaatggtttt ggagaataac ttcttcgtgg aaaccatgtt
     5341 gccatcaaaa atcatgagaa agttagaacc agaagaattt gcagcatatc ttgaaccatt
     5401 caaagagaaa ggtgaagttc gtcgtccaac attatcatgg cctcgtgaaa tcccgttagt
     5461 aaaaggtggt aaacctgacg ttgtacaaat tgttaggaat tataatgctt atctacgtgc
     5521 aagtgatgat ttaccaaaaa tgtttattga atcggaccca ggattctttt ccaatgctat
     5581 tgttgaaggt gccaagaagt ttcctaatac tgaatttgtc aaagtaaaag gtcttcattt
     5641 ttcgcaagaa gatgcacctg atgaaatggg aaaatatatc aaatcgttcg ttgagcgagt
     5701 tctcaaaaat gaacaaggaa gtggagaagg aagaggaagt ttattaacat gtggagatgt
     5761 agaagaaaat ccaggaccaa ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     5821 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     5881 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     5941 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     6001 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     6061 cgaagtgccg gggcaggatc tcctgtcatc ccaccttgct cctgccgaga aagtatccat
     6121 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     6181 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     6241 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     6301 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     6361 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     6421 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     6481 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     6541 cttctatcgc cttcttgacg agttcttctg accgcgggta ccccattaaa tttatttaat
     6601 aatagattaa aaatattata aaaataaaaa cataaacaca gaaattacaa aaaaaataca
     6661 tatgaatttt ttttttgtaa tcttccttat aaatatagaa taatgaatca tataaaacat
     6721 atcattattc atttatttac atttaaaatt attgtttcag tatctttaat ttattatgta
     6781 tatataaaaa taacttacaa ttttattaat aaacaatata tgtttattaa ttcatgtttt
     6841 gtaatttatg ggatagcgat tttttttact gtctgtattt tcttttttaa ttatgtttta
     6901 attgtattta ttttattttt attattgttc tttttatagt attattttaa aacaaaatgt
     6961 attttctaag aacttataat aataataata taaattttaa taaaaattat atttatcttt
     7021 tacaatatga acataaagta caacattaat atatagcttt taatattttt attcctaatc
     7081 atgtaaatct taaatttttc tttttaaaca tatgttaaat atttatttct cattatatat
     7141 aagaacatat ttattacatc tagaggtacc gagctcgttt tcgacactgg atggcggcgt
     7201 tagtatcgaa tcgacagcag tatagcgacc agcattcaca tacgattgac gcatgatatt
     7261 actttctgcg cacttaactt cgcatctggg cagatgatgt cgaggcgaaa aaaaatataa
     7321 atcacgctaa catttgatta aaatagaaca actacaatat aaaaaaacta tacaaatgac
     7381 aagttcttga aaacaagaat ctttttattg tcagtactga ttagaaaaac tcatcgagca
     7441 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
     7501 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
     7561 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
     7621 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
     7681 aaagcttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
     7741 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata
     7801 cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca
     7861 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg
     7921 ctgttttgcc ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat
     7981 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg
     8041 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
     8101 tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat
     8161 acccatataa atcagcatcc atgttggaat ttaatcgcgg cctcgaaacg tgagtctttt
     8221 ccttacccat ggttgtttat gttcggatgt gatgtgagaa ctgtatccta gcaagatttt
     8281 aaaaggaagt atatgaaaga agaacctcag tggcaaatcc taacctttta tatttctcta
     8341 caggggcgcg gcgtggggac aattcaacgc gtctgtgagg ggagcgtttc cctgctcgca
     8401 ggtctgcagc gaggagccgt aatttttgct tcgcgccgtg cggccatcaa aatgtatgga
     8461 tgcaaatgat tatacatggg gatgtatggg ctaaatgtac gggcgacagt cacatcatgc
     8521 ccctgagctg cgcacgtcaa gactgtcaag gagggtattc tgggcctcca tgtcgctggc
     8581 ctaacattag taatgtaggt ctgactttca ctcatataag tcttatggta actaaactaa
     8641 ggtcttacct ttactgatat atgtcttact ttcactaact taggtattac ttttactaac
     8701 ttaggtctta aattcagtaa ctaaggtcat acttcgacta actaaggtct tacattcact
     8761 gatataggtc ttatgattac taacttaggt cctaatttga ctaacataag tcctaacatt
     8821 agtaatgtag gtcttaactt aactaactta ggtcttacct tcactaatat aggtcttaat
     8881 attactgact taagtaatta aggtactaac ttaggtcgta aggtaactaa tatataggtc
     8941 ttaaggtaac taatttaggt cttgacttaa taaatatagg tcctaacata aatagtatag
     9001 gtcctaatat aagtactata ggccttaact taaccaacat aggtcctaac ataagttata
     9061 taggtcttaa cgtaactaac ataagtcatt aaggtactaa gtttggtctt aatttaacaa
     9121 taacatgtcg ctggcctaac attagtaatg taggtctgac tttcactcat ataagtctta
     9181 tggtaactaa actaaggtct tacctttact gatatatgtc ttactttcac taacttaggt
     9241 attactttta ctaacttagg tcttaaattc agtaactaag gtcatacttc gactaactaa
     9301 ggtcttacat tcactgatat aggtcttatg attactaact taggtcctaa tttgactaac
     9361 ataagtccta acattagtaa tgtaggtctt aacttaacta acttaggtct taccttcact
     9421 aatataggtc ttaatattac tgacttaagt aattaaggta ctaacttagg tcgtaaggta
     9481 actaatatat aggtcttaag gtaactaatt taggtcttga cttaataaat ataggtccta
     9541 acataaatag tataggtcct aatataagta ctataggcct taacttaacc aacataggtc
     9601 ctaacataag ttatataggt cttaacgtaa ctaacataag tcattaaggt actaagtttg
     9661 gtcttaattt aacaataacc atgtcgctgg ccgggtggtc ttaatttaac aaatatagac
     9721 catgtcgctg gccgggtgac ccggcgggga cgaggcaagc taaacagatc ctcgtgatac
     9781 gcctattttt ataggttaat gtcatgataa taatggtttc ttaggacgga tcgcttgcct
     9841 gtaacttaca cgcgcctcgt atcttttaat gatggaataa tttgggaatt tactctgtgt
     9901 ttatttattt ttatgttttg tatttggatt ttagaaagta aataaagaag gtagaagagt
     9961 tacggaatga agaaaaaaaa ataaacaaag gtttaaaaaa tttcaacaaa aagcgtactt
    10021 tacatatata tttattagac aagaaaagca gattaaatag atatacattc gattaacgat
    10081 aagtaaaatg taaaatcaca ggattttcgt gtgtggtctt ctacacagac aagatgaaac
    10141 aattcggcat taatacctga gagcaggaag agcaagataa aaggtagtat ttgttggcga
    10201 tccccctaga gtcttttaca tcttcggaaa acaaaaacta ttttttcttt aatttctttt
    10261 tttactttct atttttaatt tatatattta tattaaaaaa tttaaattat aattattttt
    10321 atagcacgtg atgaaaagga cccaggtggc acttttcggg gaaatctcga cctgcagcgt
    10381 acgaagctgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    10441 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    10501 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    10561 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    10621 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    10681 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    10741 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    10801 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    10861 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    10921 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    10981 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    11041 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    11101 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    11161 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    11221 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    11281 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    11341 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    11401 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    11461 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    11521 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
    11581 acgccaagct atttaggtga cactatagaa tactcaagct tgggg
//
