LOCUS       40924_39852       11574 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pSG230, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11574)
  AUTHORS   Goldfless SJ, Wagner JC, Niles JC.
  TITLE     Versatile control of Plasmodium falciparum gene expression with an 
            inducible protein-RNA interaction
  JOURNAL   Nat Commun 5, 5329 (2014)
  PUBMED    25370483
REFERENCE   2  (bases 1 to 11574)
  AUTHORS   Goldfless SJ, Niles JC.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-AUG-2014) Biological Engineering, MIT, 77 
            Massachusetts Avenue, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 11574)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11574)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun 
            5, 5329 (2014)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-AUG-2014) Biological Engineering, MIT, 77 Massachusetts Avenue, 
            Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      complement(24..859)
                     /label=derived from P. falciparum hsp86 3' UTR
                     /note="derived from P. falciparum hsp86 3' UTR"
                     /regulatory_class="terminator"
     CDS             complement(869..2518)
                     /label=luciferase
                     /note="firefly luciferase"
     regulatory      complement(2539..3168)
                     /note="contains promoter and 5' UTR from P. falciparum 
                     calmodulin gene"
                     /regulatory_class="promoter"
     regulatory      3169..4038
                     /note="contains promoter and 5'UTR from P. falciparum Hsp86
                     (PF07_0029)"
                     /regulatory_class="promoter"
     CDS             4049..4669
                     /label=TetR
                     /note="tetracycline repressor TetR"
     CDS             4679..4732
                     /label=T2A
                     /note="2A peptide from Thosea asigna virus capsid protein"
     CDS             4733..5668
                     /label=RLuc
                     /note="Renilla luciferase"
     misc_feature    5666..5728
                     /note="Thosea asigna virus 2A-like peptide, P. falciparum 
                     optimized codon usage"
     CDS             5675..5728
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5729..6517
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      6556..7119
                     /label=derived from P. falciparum HRPII 3' UTR
                     /note="derived from P. falciparum HRPII 3' UTR"
                     /regulatory_class="terminator"
     gene            complement(7167..8523)
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     repeat_region   8531..9069
     repeat_region   9070..9624
     misc_feature    complement(9778..10281)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     rep_origin      10532..11120
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    11408..11429
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        11444..11474
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11482..11498
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11506..11522
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11540..11558
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 ggatcctcta gagtcgacct gcaggcatgc tatttgatga attaactaca cttaaaataa
       61 tacaattatt attaaatttt tttttgattt atttattaat ttttaaactt aatcatttgt
      121 atttgggagg aattatatat atctttataa ttattttatt tttttttatt tttttatttt
      181 tttattatta ttattttttt ttattttttt tttttactgt atcaaagaaa aacctttaaa
      241 aaaaaaatta taatttcccc atcttactat atttttaata catacgtttt aaggaattaa
      301 attagacaaa agctatatta tgctttacat ataattagaa tttataaacg tttggttatt
      361 agatatttca tgtctcagta aagtctttca atacatatgt aaaaaaatat atatgaatac
      421 acataagttg ttaatatatt ttatatgcat aaatgtataa atatatatat atatatatat
      481 atatgtatgt atgtatatgt gtgtatatga aattatttca atgtttaatt ttttaaattt
      541 taattttttt tttttttttt ttttttatta tgtatattga tctttattat ttaaatatta
      601 cttttttcgt tttttcttct ttttattatt tttttttttt tttatatttt atacaaatgg
      661 taattcaaat aaaaggtata aatttatatt taattttctt ttatggataa ataaaagaaa
      721 aatataaata tataaaaata taaaaatata tatatgtata ttggggtgat gataaaatga
      781 aagataatat atatatatat atatctttat tttttttttt ttgtagaccc cattgtgagt
      841 acataaatat attatataac tcgagttaca actcggactt tccgcccttc ttggccttta
      901 tgaggatctc tctgattttt cttgcgtcga gttttccggt aagacctttc ggtacttcgt
      961 ccacaaacac aactcctccg cgcaactttt tcgcggttgt tacttgactg gcgacgtaat
     1021 ccacgatctc tttttccgtc atcgtctttc cgtgctccaa aacaacaacg gcggcgggaa
     1081 gttcaccggc gtcatcgtcg ggaagacctg ccacgcccgc gtcgaagatg ttggggtgtt
     1141 gtaacaatat cgattccaat tcagcggggg ccacctgata tcctttgtat ttaattaaag
     1201 acttcaagcg gtcaactatg aagaagtgtt cgtcttcgtc ccagtaagct atgtctccag
     1261 aatgtagcca tccatccttg tcaatcaagg cgttggtcgc ttccggattg tttacataac
     1321 cggacataat cataggtcct ctgacacata attcgcctct ctgattaacg cccagcgttt
     1381 tcccggtatc cagatccaca accttcgctt caaaaaatgg aacaacttta ccgaccgcgc
     1441 ccggtttatc atccccctcg ggtgtaatca gaatagctga tgtagtctca gtgagcccat
     1501 atccttgtcg tatccctgga agatggaagc gttttgcaac cgcttccccg acttctttcg
     1561 aaagaggtgc gcccccagaa gcaatttcgt gtaaattaga taaatcgtat ttgtcaatca
     1621 gagtgctttt ggcgaagaat gaaaataggg ttggtactag caacgcactt tgaattttgt
     1681 aatcctgaag ggatcgtaaa aacagctctt cttcaaatct atacattaag acgactcgaa
     1741 atccacatat caaatatccg agtgtagtaa acattccaaa accgtgatgg aatggaacaa
     1801 cacttaaaat cgcagtatcc ggaatgattt gattgccaaa aataggatct ctggcatgcg
     1861 agaatctgac gcaggcagtt ctatgcggaa gggccacacc cttaggtaac ccagtagatc
     1921 cagaggaatt cattatcagt gcaattgttt tgtcacgatc aaaggactct ggtacaaaat
     1981 cgtattcatt aaaaccggga ggtagatgag atgtgacgaa cgtgtacatc gactgaaatc
     2041 cctggtaatc cgttttagaa tccatgataa taattttctg gattattggt aatttttttt
     2101 gcacgttcaa aattttttgc aacccctttt tggaaacaaa cactacggta ggctgcgaaa
     2161 tgttcatact gttgagcaat tcacgttcat tataaatgtc gttcgcgggc gcaactgcaa
     2221 ctccgataaa taacgcgccc aacaccggca taaagaattg aagagagttt tcactgcata
     2281 cgacgattct gtgatttgta ttcagcccat atcgtttcat agcttctgcc aaccgaacgg
     2341 acatttcgaa gtattccgcg tacgtgatgt tcacctcgat atgtgcatct gtaaaagcaa
     2401 ttgttccagg aaccagggcg tatctcttca tagccttatg cagttgctct ccagcggttc
     2461 catcctctag tggatagaat ggcgccgggc ctttctttat gtttttggcg tcttccatca
     2521 gacgtctccc ccgggaccat atatttctat taggtattta ttattataaa atataaatct
     2581 tgaatgataa taaataaaat attagttatt ccttttctag tttaaaatat acatattata
     2641 aatatatata tatatatata tatttttatt gtgacaagaa tatataatta taaattatat
     2701 tatttatttt tgtatttttt tttttttttt tttttttttc tttttttgtt ttatttttct
     2761 ttttttttat aaatattatt tttttctttt atcatgcaca ttggaataat acattaatat
     2821 atatatatat attatattat acatatattg aataatgttt ataaaaaatg cataacttat
     2881 atgaatataa ttttttttaa atatgacaaa aagaaaaaaa aaaaaaacca aaaaaaatta
     2941 aaattgaaat gaaatatata aatatattat ttatatatat tatacattgt ttaatactac
     3001 tacatgtata tatatatatt atatatatat atatatatca attttttcaa aaataaatta
     3061 atataaaaag aggggaaaaa aaaaaaaaaa aaaaaaaaaa gataattaag taagcattta
     3121 aaaatatata aattgataat atataaaatt aatcacatat aaaagcttcg gaaaggggcc
     3181 attggatata tatttagtat tccatagtat atagttaaat ataataatat tatagtaaat
     3241 ttttgcatga atttaaagaa aaaaaaaaaa aaaataacaa aaaggagtat tatataaata
     3301 taacaaaata ataaatacta tacctatata tatatatata tatatataca tatatataaa
     3361 tatataaata tataaatata tttatataaa aataatattt tatatataat attatatttt
     3421 tataatatct tttttttttt aaatatcatc tttgtttctt tctttctttc tttctttctt
     3481 tctttttttt tttttttgat ttatatatta ttttattttg tatttacaaa atataataaa
     3541 ataatttcat gtttagcaaa aattttaaat aaaaaataat aatataaaat aaaaagcatc
     3601 atttaatata aaataataaa attaaaataa aataagataa ttaccaaaaa taataaaaaa
     3661 aattaaaaag tattaaaaaa tatatatcat atataaatat ataaatttta acaacgtgag
     3721 aaattaagaa aaaaattata tttatataaa taatataata tttaatatat atctcatata
     3781 atattatata tatatatata tatatatata tattttttaa aaaaaaagaa aaaataagtt
     3841 tatttttacc ttttaataaa aacatatttt tagatataca taaaaatttt tatattattt
     3901 tattggtata tatatatata tatatatata taataatata tatataataa aatatataaa
     3961 tatttacata aatattttac tttaaaaaga aattttcctt ttttattata ttttttttct
     4021 tcccacattt cgaataaacc taggaaaaat gtctagatta gataaaagta aagtgattaa
     4081 cagcgcatta gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc
     4141 ccagaagctt ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt
     4201 gctcgacgcc ttagccattg agatgttaga taggcaccat actcacttct gccctttaga
     4261 aggggaaagc tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact
     4321 aagtcatcgc gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga
     4381 aactctcgaa aatcaattag cctttttatg ccaacaaggt ttttcactag agaacgcgtt
     4441 atatgcactc agcgctgtgg ggcattttac cttaggttgc gtattggaag atcaagagca
     4501 tcaagtcgct aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg
     4561 acaagctatc gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga
     4621 attgatcata tgcggattag aaaaacaact taaatgtgaa agtgggtctg gtagtggtga
     4681 aggtagagga tcattattaa catgtggtga tgttgaagaa aatccaggtc caatgacttc
     4741 gaaagtttat gatccagaac aaaggaaacg gatgataact ggtccgcagt ggtgggccag
     4801 atgtaaacaa atgaatgttc ttgattcatt tattaattat tatgattcag aaaaacatgc
     4861 agaaaatgct gttatttttt tacatggtaa cgcggcctct tcttatttat ggcgacatgt
     4921 tgtgccacat attgagccag tagcgcggtg tattatacca gaccttattg gtatgggcaa
     4981 atcaggcaaa tctggtaatg gttcttatag gttacttgat cattacaaat atcttactgc
     5041 atggtttgaa cttcttaatt taccaaagaa gatcattttt gtcggccatg attggggtgc
     5101 ttgtttggca tttcattata gctatgagca tcaagataag atcaaagcaa tagttcacgc
     5161 tgaaagtgta gtagatgtga ttgaatcatg ggatgaatgg cctgatattg aagaagatat
     5221 tgcgttgatc aaatctgaag aaggagaaaa aatggttttg gagaataact tcttcgtgga
     5281 aaccatgttg ccatcaaaaa tcatgagaaa gttagaacca gaagaatttg cagcatatct
     5341 tgaaccattc aaagagaaag gtgaagttcg tcgtccaaca ttatcatggc ctcgtgaaat
     5401 cccgttagta aaaggtggta aacctgacgt tgtacaaatt gttaggaatt ataatgctta
     5461 tctacgtgca agtgatgatt taccaaaaat gtttattgaa tcggacccag gattcttttc
     5521 caatgctatt gttgaaggtg ccaagaagtt tcctaatact gaatttgtca aagtaaaagg
     5581 tcttcatttt tcgcaagaag atgcacctga tgaaatggga aaatatatca aatcgttcgt
     5641 tgagcgagtt ctcaaaaatg aacaaggaag tggagaagga agaggaagtt tattaacatg
     5701 tggagatgta gaagaaaatc caggaccaat tgaacaagat ggattgcacg caggttctcc
     5761 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     5821 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     5881 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     5941 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     6001 gctattgggc gaagtgccgg ggcaggatct cctgtcatcc caccttgctc ctgccgagaa
     6061 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     6121 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     6181 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     6241 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     6301 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     6361 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     6421 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     6481 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga ccgcgggtac cccattaaat
     6541 ttatttaata atagattaaa aatattataa aaataaaaac ataaacacag aaattacaaa
     6601 aaaaatacat atgaattttt tttttgtaat cttccttata aatatagaat aatgaatcat
     6661 ataaaacata tcattattca tttatttaca tttaaaatta ttgtttcagt atctttaatt
     6721 tattatgtat atataaaaat aacttacaat tttattaata aacaatatat gtttattaat
     6781 tcatgttttg taatttatgg gatagcgatt ttttttactg tctgtatttt cttttttaat
     6841 tatgttttaa ttgtatttat tttattttta ttattgttct ttttatagta ttattttaaa
     6901 acaaaatgta ttttctaaga acttataata ataataatat aaattttaat aaaaattata
     6961 tttatctttt acaatatgaa cataaagtac aacattaata tatagctttt aatattttta
     7021 ttcctaatca tgtaaatctt aaatttttct ttttaaacat atgttaaata tttatttctc
     7081 attatatata agaacatatt tattacatct agaggtaccg agctcgtttt cgacactgga
     7141 tggcggcgtt agtatcgaat cgacagcagt atagcgacca gcattcacat acgattgacg
     7201 catgatatta ctttctgcgc acttaacttc gcatctgggc agatgatgtc gaggcgaaaa
     7261 aaaatataaa tcacgctaac atttgattaa aatagaacaa ctacaatata aaaaaactat
     7321 acaaatgaca agttcttgaa aacaagaatc tttttattgt cagtactgat tagaaaaact
     7381 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
     7441 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
     7501 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
     7561 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
     7621 agaatggcaa aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct
     7681 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
     7741 gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
     7801 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
     7861 cctggaatgc tgttttgccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
     7921 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
     7981 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
     8041 catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
     8101 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctcgaaacgt
     8161 gagtcttttc cttacccatg gttgtttatg ttcggatgtg atgtgagaac tgtatcctag
     8221 caagatttta aaaggaagta tatgaaagaa gaacctcagt ggcaaatcct aaccttttat
     8281 atttctctac aggggcgcgg cgtggggaca attcaacgcg tctgtgaggg gagcgtttcc
     8341 ctgctcgcag gtctgcagcg aggagccgta atttttgctt cgcgccgtgc ggccatcaaa
     8401 atgtatggat gcaaatgatt atacatgggg atgtatgggc taaatgtacg ggcgacagtc
     8461 acatcatgcc cctgagctgc gcacgtcaag actgtcaagg agggtattct gggcctccat
     8521 gtcgctggcc taacattagt aatgtaggtc tgactttcac tcatataagt cttatggtaa
     8581 ctaaactaag gtcttacctt tactgatata tgtcttactt tcactaactt aggtattact
     8641 tttactaact taggtcttaa attcagtaac taaggtcata cttcgactaa ctaaggtctt
     8701 acattcactg atataggtct tatgattact aacttaggtc ctaatttgac taacataagt
     8761 cctaacatta gtaatgtagg tcttaactta actaacttag gtcttacctt cactaatata
     8821 ggtcttaata ttactgactt aagtaattaa ggtactaact taggtcgtaa ggtaactaat
     8881 atataggtct taaggtaact aatttaggtc ttgacttaat aaatataggt cctaacataa
     8941 atagtatagg tcctaatata agtactatag gccttaactt aaccaacata ggtcctaaca
     9001 taagttatat aggtcttaac gtaactaaca taagtcatta aggtactaag tttggtctta
     9061 atttaacaat aacatgtcgc tggcctaaca ttagtaatgt aggtctgact ttcactcata
     9121 taagtcttat ggtaactaaa ctaaggtctt acctttactg atatatgtct tactttcact
     9181 aacttaggta ttacttttac taacttaggt cttaaattca gtaactaagg tcatacttcg
     9241 actaactaag gtcttacatt cactgatata ggtcttatga ttactaactt aggtcctaat
     9301 ttgactaaca taagtcctaa cattagtaat gtaggtctta acttaactaa cttaggtctt
     9361 accttcacta atataggtct taatattact gacttaagta attaaggtac taacttaggt
     9421 cgtaaggtaa ctaatatata ggtcttaagg taactaattt aggtcttgac ttaataaata
     9481 taggtcctaa cataaatagt ataggtccta atataagtac tataggcctt aacttaacca
     9541 acataggtcc taacataagt tatataggtc ttaacgtaac taacataagt cattaaggta
     9601 ctaagtttgg tcttaattta acaataacca tgtcgctggc cgggtggtct taatttaaca
     9661 aatatagacc atgtcgctgg ccgggtgacc cggcggggac gaggcaagct aaacagatcc
     9721 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct taggacggat
     9781 cgcttgcctg taacttacac gcgcctcgta tcttttaatg atggaataat ttgggaattt
     9841 actctgtgtt tatttatttt tatgttttgt atttggattt tagaaagtaa ataaagaagg
     9901 tagaagagtt acggaatgaa gaaaaaaaaa taaacaaagg tttaaaaaat ttcaacaaaa
     9961 agcgtacttt acatatatat ttattagaca agaaaagcag attaaataga tatacattcg
    10021 attaacgata agtaaaatgt aaaatcacag gattttcgtg tgtggtcttc tacacagaca
    10081 agatgaaaca attcggcatt aatacctgag agcaggaaga gcaagataaa aggtagtatt
    10141 tgttggcgat ccccctagag tcttttacat cttcggaaaa caaaaactat tttttcttta
    10201 atttcttttt ttactttcta tttttaattt atatatttat attaaaaaat ttaaattata
    10261 attattttta tagcacgtga tgaaaaggac ccaggtggca cttttcgggg aaatctcgac
    10321 ctgcagcgta cgaagctgcc tcactgatta agcattggta actgtcagac caagtttact
    10381 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
    10441 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
    10501 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
    10561 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
    10621 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
    10681 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
    10741 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
    10801 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    10861 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
    10921 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
    10981 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    11041 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    11101 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
    11161 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
    11221 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
    11281 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
    11341 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
    11401 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
    11461 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
    11521 accatgatta cgccaagcta tttaggtgac actatagaat actcaagctt gggg
//
