LOCUS       62056_19650        3290 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      987..1575
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(1761..1901)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2006..2194)
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             join(3279..3290,1..813)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="PCRVAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILE
                     SFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGM
                     TVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPND
                     ERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKS
                     GAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
ORIGIN
        1 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
       61 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      121 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      181 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
      241 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      301 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      361 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      421 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      481 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
      541 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
      601 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
      661 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
      721 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
      781 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
      841 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
      901 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
      961 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1021 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1081 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     1141 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1201 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1261 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1321 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1381 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1441 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1501 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1561 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1621 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     1681 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     1741 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     1801 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     1861 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
     1921 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     1981 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
     2041 agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat
     2101 ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg
     2161 ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc
     2221 tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg
     2281 atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc
     2341 ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag
     2401 gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg
     2461 gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg
     2521 ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg
     2581 attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga
     2641 gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc
     2701 tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag
     2761 ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc
     2821 gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag
     2881 gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga
     2941 ggcggcataa atcgccgtga cgatcagcgg tccaatgatc gaagttaggc tggtaagagc
     3001 cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc
     3061 ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat
     3121 ccagcctcgc gtcgcgacta agaaaatgcc gtcaaatccg ctcgccatga cttcactaac
     3181 gatgcctttg aaaatcttca agttcttttc tactaattca aggcgtgtct caccaggttt
     3241 ttggtttgct ccggcgcaaa tgcagacaat atcagcatcc ttgcagggta
//
