LOCUS       V014364                 8440 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014364
VERSION     V014364
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8440)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(185..325)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(430..618)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     CDS             3081..3860
                     /gene="ant1"
                     /label="Spectinomycin 9-adenylyltransferase"
                     /note="Spectinomycin 9-adenylyltransferase from
                     Staphylococcus aureus (strain N315). Accession#: P0A0D1"
     CDS             4317..5051
                     /note="Isopentenyl phosphate kinase from Thermoplasma
                     acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC
                     15155 / AMRC-C165). Accession#: Q9HLX1"
                     /label="Isopentenyl phosphate kinase"
     CDS             5076..5789
                     /label="GFP"
                     /note="green fluorescent protein"
     primer_bind     complement(5807..5823)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6715..6819
                     /label="AmpR promoter"
     CDS             6820..7677
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7851..8439
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
       61 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
      121 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
      181 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat
      661 gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg
      721 gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa
      781 aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag
      841 ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt
      901 ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga
      961 cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc
     1021 agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac
     1081 ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga
     1141 cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg
     1201 attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag
     1261 gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg
     1321 gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc
     1381 gtgacgatca gcggtccaat gatcgaagtt aggctggtaa gagccgcgag cgatccttga
     1441 agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc
     1501 ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg
     1561 actaagaaaa tgccgtcaaa tccgctcgcc atgacttcac taacgatgcc tttgaaaatc
     1621 ttcaagttct tttctactaa ttcaaggcgt gtctcaccag gtttttggtt tgctccggcg
     1681 caaatgcaga caatatcagc atccttgcag ggtatgtttc tctttgatgt ctttttgttt
     1741 gtgaagtatt tcacatttat attgtgcaac acttcacaaa cttttgcaag agaaaagttt
     1801 tgtctgattt atgaacaaaa aagaaaccat cattgatggt ttctttcggt aagtcccgtc
     1861 tagccttgcc ctcaatgggg aagagaaccg cttaagcccg agtcattata taaaccattt
     1921 agcacgtaat caaagccagg ctgattctga ccgggcactt gggcgctgcc attattaaaa
     1981 atcacttttg cgttggttgt atccgtgtcc gcaggcagcg tcagcgtgta aattccgtct
     2041 gcatttttag tcattggttt tccaggccaa gatccggtca attcaattac tcggctccca
     2101 tcatgtttat agatataagc atttacctgg ctccaatgat tcggattttg atagccgatg
     2161 gttttggccg acgctggatc tcttttaaca aaactgtatt tctcggtcct cgttacacca
     2221 tcactgttcg ttccttttaa catgatggtg tatgttttgc caaatggatc tccttttccg
     2281 attgtgaatt gatctccatc cttaaacgcc gtctctggtc cattattgat ttgataaacg
     2341 gcttttgttg tattcgcatc tgcacgcaag gtaatcgtca gttgatcatt gaaagaatgt
     2401 gttacacctg ttttgtaatt ctcaaggaaa acatgaggcg cttttgcaat atcatcagga
     2461 taaagcacag ctacagacct ggcattgatc gtgcctgtca gtttaccatc gttcacttga
     2521 aatgaacccg ctccagcttt attgtcatac ctgccatcag gcaattttgt tgccgtattg
     2581 atagagacag aggatgaacc tgcatttgcc agcacaacgc catgtgagcc gcgctgattc
     2641 ataaatatct ggttgtttcc attcgggttc gagagttcct caggctgtcc agccatcaca
     2701 ttgtgaaatc tattgaccgc agtgatagcc tgatcttcaa ataaagcact cccgcgatcg
     2761 cctatttggc ttttccccgg gaacctcaca ccatttccgc ctccctcagg tctggaaaag
     2821 aaaagaggcg tactgcctga acgagaagct atcaccgccc agcctaaacg gatatcatca
     2881 tcgctcatcc atgtcgactc tagaggatcg atctgtataa taaagaataa ttattaatct
     2941 gtagacaaat tgtgaaagga tgtacttaaa cgctaacggt cagctttatt gaacagtaat
     3001 ttaagtatat gtccaatcta gggtaagtaa attgagtatc aatataaact ttatatgaac
     3061 ataatcaacg aggtgaaatc atgagcaatt tgattaacgg aaaaatacca aatcaagcga
     3121 ttcaaacatt aaaaatcgta aaagatttat ttggaagttc aatagttgga gtatatctat
     3181 ttggttcagc agtaaatggt ggtttacgca ttaacagcga tgtagatgtt ctagtcgtcg
     3241 tgaatcatag tttacctcaa ttaactcgaa aaaaactaac agaaagacta atgactatat
     3301 caggaaagat tggaaatacg gattctgtta gaccacttga agttacggtt ataaatagga
     3361 gtgaagttgt cccttggcaa tatcctccaa aaagagaatt tatatacggt gagtggctca
     3421 ggggtgaatt tgagaatgga caaattcagg aaccaagcta tgatcctgat ttggctattg
     3481 ttttagcaca agcaagaaag aatagtattt ctctatttgg tcctgattct tcaagtatac
     3541 ttgtctccgt acctttgaca gatattcgaa gagcaattaa ggattctttg ccagaactaa
     3601 ttgaggggat aaaaggtgat gagcgtaatg taattttaac cctagctcga atgtggcaaa
     3661 cagtgactac tggtgaaatt acctcgaaag atgtcgctgc agaatgggct atacctcttt
     3721 tacctaaaga gcatgtaact ttactggata tagctagaaa aggctatcgg ggagagtgtg
     3781 atgataagtg ggaaggacta tattcaaagg tgaaagcact cgttaagtat atgaaaaatt
     3841 ctatagaaac ttctctcaat taggctaatt ttattgcaat aacaggtgct tacttttaaa
     3901 actactgatt tattgataaa tattgaacaa tttttgggaa gaataaagcg tcctcttgtg
     3961 aaattagaga acgctttatt actttaattt agtgaaacaa tttgtaacta ttgaaaatag
     4021 aaagaaattg ttccttcgat agtttattaa tattagtgac atttgcatgc ttcaaagcct
     4081 gtcggaattg gtttttagtg aaaagaacac gatattcacg gtttacccac ttataaacaa
     4141 aagatcggat cttcatgaaa aactaaaaaa aatattgaaa atactgatga ggttatttaa
     4201 gattaaaata agttagtttg tttgggcaac aaactaatgt gcaacttact tacaatatga
     4261 cataaaatgc atctagaaag gagattccta ggatgggtac catggatcct ttcacaatga
     4321 tgatccttaa aatcggcggc tctgttatca cagataaatc tgcttaccgt acagctcgta
     4381 catacgctat ccgttctatc gttaaagttc tttctggcat cgaagatctt gtttgcgttg
     4441 ttcatggcgg cggctctttc ggccatatca aagctatgga attcggcctt cctggcccta
     4501 aaaaccctcg ttcttctatc ggctactcta tcgttcatcg tgatatggaa aaccttgatc
     4561 ttatggttat cgatgctatg atcgaaatgg gcatgcgtcc tatctctgtt cctatctctg
     4621 ctcttcgtta tgacggcagg ttcgattaca caccacttat ccgttacatc gatgctggct
     4681 tcgttcctgt ttcttacggc gatgtttaca tcaaagatga acattcttac ggcatctact
     4741 ctggcgatga tatcatggct gatatggctg aacttcttaa acctgatgtt gctgttttcc
     4801 ttacagatgt tgatggcatc tactctaaag atcctaaacg taaccctgat gctgttcttc
     4861 ttcgtgatat cgatacaaac atcacattcg atcgtgttca aaacgatgtt acaggcggca
     4921 tcggcaaaaa attcgaatct atggttaaaa tgaaatcttc tgttaagaat ggcgtgtacc
     4981 tgatcaacgg caaccatcct gaacgtatcg gcgatatcgg caaagaatct ttcatcggca
     5041 cagttatccg taagcttgat atcgaattcc tgcagatgag taaaggagaa gaacttttca
     5101 ctggagttgt cccaattctt gttgaattag atggtgacgt taatgggcac aaattttctg
     5161 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca
     5221 ctactggaaa actacctgtt ccatggccaa cacttgtcac tactctgact tatggtgttc
     5281 aatgcttttc aagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc
     5341 ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac
     5401 gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg
     5461 attttaaaga agatggaaac attcttggac acaaattgga atacaactat aactcacaca
     5521 atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac
     5581 acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg
     5641 gcgatggccc tgtcctttca ccagacaacc attacctgtc cacacaatct gccctttcga
     5701 aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga
     5761 ttacacatgg catggatgaa ctatacaaat aaatgtccag acttcagatc cactagttct
     5821 agagcggccg cgaattatca agctctgagc gccggtcgct accattacca gttggtctgg
     5881 tgtcaaaaat aataataacc gggcaggcca tgtctgcccg tatttcgcgt aaggaaatcc
     5941 attatgtact atttaattct gcgtgacatc ccatcgatca gaccagtttt taatttgtgt
     6001 gtttccatgt gtccagtttg gaatactctt aacctcattg gaaatcgcgg cataatcact
     6061 ggtggtatga ttgatgaccg cgtcaacaat gacctttatg ccatattctt cagcggctgc
     6121 acacatttct ttaaattctt gttcagtacc taagtaacgg ttgccaattt gatacgatgt
     6181 cggctgatac agccagtacc agttcgacat gcttttatct ccttgattcc cttcctttac
     6241 ttggttaatc ggagatgtct gaatggctgt atatcctgca tcatgaatat ccttcatatt
     6301 gtgttttaac gtattgaacg accaattcca tgcatgaaga atggttccgc ttttgatcga
     6361 cggtgctgta agctcattcg atttgttcgc cgtttcagca ctcgcagccg ccggtcctgc
     6421 cagaaccaaa tgaaacagca ataaaaatcc agcgaataac ggcagtaaag aggttttgaa
     6481 tcgttttgca aacattcttg acactcctta ttcgattttt tgaagactta cttcggagtc
     6541 aaaaatccct cttacttcat tcttccgctt cctcctttca aaccgatgtg aagactggag
     6601 aattttgtta attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     6661 gtcatgataa taatggtttc ttagacgtca ggcggcactt ttcggggaaa tgtgcgcgga
     6721 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     6781 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     6841 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     6901 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     6961 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     7021 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     7081 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     7141 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     7201 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     7261 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     7321 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
     7381 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     7441 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     7501 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     7561 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     7621 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     7681 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     7741 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     7801 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     7861 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     7921 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     7981 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     8041 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     8101 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     8161 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     8221 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     8281 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     8341 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     8401 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
//
