LOCUS       V003278                 6444 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003278
VERSION     V003278
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6444)
  AUTHORS   Galuska S, Donald RGK., Muise E, Wiersma HI, Diaz C, Kulkarni A,
            Cavet G, Roemer T, Liberator P, Douglas C.
  TITLE     A Candida albicans GFP reporter assay for identifying inhibitors of
            cell wall biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6444)
  AUTHORS   Galuska S.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6444)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6444)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-MAR-2009) Infectious Diseases, Merck "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             241..1653
                     /codon_start=1
                     /product="ARG4"
                     /label="ARG4"
                     /note="contains unique NotI site"
                     /protein_id="ADD22644.1"
                     /translation="MSQQQDKQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADL
                     TGTKVYTQGLNKLGLITTEELHLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGE
                     IIGKNISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMP
                     GYTHLQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREF
                     LAKGLGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLA
                     AADAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEP
                     LFDALTTVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHI
                     SGECVRKAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDAIGGTAKSAV
                     LKQLENLKSILS"
     misc_feature    1081..1088
                     /note="NotI site; inserted in ARG4 to facilitate targeted
                     integration at the ARG4 locus of Candida albicans
                     transformants"
     primer_bind     1910..1926
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             1972..2676
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     primer_bind     complement(2864..2880)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2888..2904)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2912..2942)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2957..2978)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3266..3854)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4028..4885)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4886..4990)
                     /label="AmpR promoter"
     CDS             5435..6244
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 atgtcacaac aacaagataa acaacctagt gaaaataaat tatggggtgg tcgtttcacc
      301 ggtgccactg atccattgat ggatttatat aatgcttcat taccttatga taaagttatg
      361 tatgatgctg atttaactgg tactaaagta tacacacaag gattaaacaa attaggatta
      421 attaccactg aagaattgca tttgattcat caaggattag aacaaatcag acaagaatgg
      481 catgataata aattcattat taaggcaggt gatgaggata ttcatactgc taatgaacgt
      541 cgattaggag aaatcattgg gaaaaacatt tctggtaaag ttcacactgg tagatcaaga
      601 aatgatcaag ttgctaccga tatgagaatt ttcgttcgtg aatcattact taatttaagt
      661 aaaattttac atcaattcat cacggcaatt cttgaacggg cacataaaga aatcgatgta
      721 ttaatgcctg gttatactca tttacaaaaa gctcaaccta ttagatgggc acattggtta
      781 agttcatatg caacttattt caccgaagat tataaacgtt tacaagaaat tataactcgt
      841 gttaaccaat ctccattagg atctggtgct ttagcaggtc atccttatgg tattgatcgt
      901 gaatttttag ctaaaggatt aggatttgat ggtgtcattg ggaattcttt aactgcagtt
      961 tcagatcgtg attttgttgt tgaaagttta ttttggagta cattatttat gaatcatatt
     1021 tctcgattct cagaagatct aattatttat tctagtggtg aatttgggtt cataaaatta
     1081 gcggccgctg atgcttattc tactggttct tcattgatgc cacaaaagaa aaaccctgac
     1141 tcattagaat tattacgtgg gaaaagtggt agagtatttg gtcaattatc tggattttta
     1201 atgtcgatca aatctattcc atctacttat aataaagata tgcaagagga taaagaacca
     1261 ttatttgatg cattgactac agtggaacat tcaattttaa ttgctactgg tgtgatttct
     1321 actttactga ttgataaaca aaatatggaa aaagctttaa ctatggatat gttggctact
     1381 gatttagctg attatttagt tagaaaagga gttccattta gagaaactca tcatatatct
     1441 ggtgaatgtg ttagaaaagc tgaagaagaa aaattatctg gaattgatca attatctttt
     1501 gaacaatttc aacaaattga ttctcgtttt gaaaaagacg ttatggaaac ttttgatttt
     1561 gaagctagtg tggaaagaag agatgctatt ggtggtactg ctaaaagtgc cgttttaaaa
     1621 caattagaaa atttaaaatc aattttaagt taatttataa atagtcatat aataatcaca
     1681 gtattgttta acttacataa aatataatca attaacacag agataccttg tactccaata
     1741 ctagtgttat aatgtttata acagcggcgc cattcgccat tcaggctgcg caactgttgg
     1801 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     1861 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     1921 gccagtgaat tcgagctcgg tacccggggg cgcgccggat ccttaattaa cggtgaagaa
     1981 ttattcactg gtgttgtccc aattttggtt gaattagatg gtgatgttaa tggtcacaaa
     2041 ttttctgtct ccggtgaagg tgaaggtgat gctacttacg gtaaattgac cttaaaattt
     2101 atttgtacta ctggtaaatt gccagttcca tggccaacct tagtcactac tttcggttat
     2161 ggtgttcaat gttttgctag atacccagat catatgaaac aacatgactt tttcaagtct
     2221 gccatgccag aaggttatgt tcaagaaaga actatttttt tcaaagatga cggtaactac
     2281 aagaccagag ctgaagtcaa gtttgaaggt gataccttag ttaatagaat cgaattaaaa
     2341 ggtattgatt ttaaagaaga tggtaacatt ttaggtcaca aattggaata caactataac
     2401 tctcacaatg tttacatcat ggctgacaaa caaaagaatg gtatcaaagt taacttcaaa
     2461 attagacaca acattgaaga tggttctgtt caattagctg accattatca acaaaatact
     2521 ccaattggtg atggtccagt cttgttacca gacaaccatt acttatccac tcaatctgcc
     2581 ttatccaaag atccaaacga aaagagagac cacatggtct tgttagaatt tgttactgct
     2641 gctggtatta cccatggtat ggatgaattg tacaaataaa atgatatttt tagatatata
     2701 taattatatt tatatttata tttgtatatt gagaaattgt attttgttgt tatagtttag
     2761 aagaaatgag attagagtcc tgtagtataa gagattcaca aagtattgcc catactattc
     2821 ttttggttta aacctgcagg catgcaagct tggcgtaatc atggtcatag ctgtttcctg
     2881 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     2941 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     3001 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     3061 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     3121 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     3181 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     3241 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3301 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3361 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3421 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3481 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3541 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3601 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3661 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     3721 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3781 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3841 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3901 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3961 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     4021 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     4081 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     4141 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     4201 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     4261 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     4321 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     4381 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     4441 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     4501 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     4561 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     4621 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     4681 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     4741 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     4801 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     4861 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     4921 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     4981 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cggaattgat ttggatggta
     5041 taaacggaaa caaaaaaaag agctggtact actttcttta aaattatttt attatttgat
     5101 tttatttaat agtatatatt atattttgaa cgtagattat tttgttgaaa gttgctgtag
     5161 tgccattgat tcgtaacact aattctgtat tagtcattcc tcttgtttga tagtatccaa
     5221 aaaaacggct atttttttgc aatcttattt cctgcatatt atacagataa cataatgaaa
     5281 gaaaaaatct ttttttttgt tcttcaatga tgatttcaac cattctttta aacattgatc
     5341 aattcctgag caacaacccc atacacactg gtttatatat cgcccctttt acagttgaag
     5401 aaagaaatag aaatagaaat agcaaacaaa agatatgaca gtcaacacta agacctatag
     5461 tgagagagca gaaactcatg cctcaccagt agcacagcga ttatttcgat taatggaact
     5521 gaagaaaacc aatttatgtg catcaattga cgttgatacc actaaggaat tccttgaatt
     5581 aattgataaa ttaggtcctt atgtatgctt aatcaagact catattgata taatcaatga
     5641 tttttcctat gaatccacta ttgaaccatt attagaactt tcacgtaaac atcaatttat
     5701 gatttttgaa gatagaaaat ttgctgatat tggtaatacc gtaaagaaac aatatattgg
     5761 tggagtttat aaaatcagta gttgggcaga tattaccaat gctcatggtg tcactgggaa
     5821 tggagtggtt gaaggattaa aacagggagc taaagaaacc accaccaacc aagagccaag
     5881 agggttattg atgttagctg aattatcatc agtgggatca ttagcatatg gagaatattc
     5941 tcaaaaaact gttgaaattg ctaaatccga taaggaattt gttattggat ttattgccca
     6001 acgtgatatg ggtggccaag aagaaggatt tgattggctt attatgacac ctggagttgg
     6061 attagatgat aaaggtgatg gattaggaca acaatataga actgttgatg aagttgttag
     6121 cactggaact gatattatca ttgttggtag aggattgttt ggtaaaggaa gagatccaga
     6181 tattgaaggt aaaaggtata gaaatgctgg ttggaatgct tatttgaaaa agactggcca
     6241 attataaatg tgaaggggga gattttcact ttattagatt tgtatatatg tagaataaat
     6301 aaataaataa gttaaataaa taattaaata agggtggtaa ttattactat ttacaatcaa
     6361 aggtggtcct tctagacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     6421 ggcgtatcac gaggcccttt cgtc
//
