LOCUS       V003279                13979 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003279
VERSION     V003279
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13979)
  AUTHORS   Mattiuzzo G, Ashall J, Doris KS, MacLellan-Gibson K, Nicolson C,
            Wilkinson DE, Harvey R, Almond N, Anderson R, Efstathiou S, Minor
            PD, Page M.
  TITLE     Development of Lentivirus-Based Reference Materials for Ebola Virus
            Nucleic Acid Amplification Technology-Based Assays
  JOURNAL   PLoS ONE 10 (11), E0142751 (2015)
   PUBMED   26562415
REFERENCE   2  (bases 1 to 13979)
  AUTHORS   Mattiuzzo G, Mitchell J, Mee ET.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUN-2015) Virology, National Institute for Biological
            Standards and Controls, Blanche Lane, South Mimms-Potters Bar EN6
            3QG, United Kingdom
REFERENCE   3  (bases 1 to 13979)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13979)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Geneious v. 7.1.8
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2015"; volume: "10"; issue: "11"; pages: "E0142751"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (19-JUN-2015) Virology, National Institute for Biological Standards
            and Controls, Blanche Lane, South Mimms-Potters Bar EN6 3QG, United
            Kingdom"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13979
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     601..617
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        624..642
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        713..1016
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1017..1220
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             1235..1415
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1462..1587
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    2080..2313
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             2498..2542
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2691..2732
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2809..2925
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     misc_feature    3001..3092
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     CDS             complement(3538..4515)
                     /gene="VP40"
                     /label="Matrix protein VP40"
                     /note="Matrix protein VP40 from Zaire ebolavirus (strain
                     Kikwit-95). Accession#: Q77DJ6"
     gene            complement(4613..11235)
                     /gene="L"
                     /label="L"
     LTR             11337..11570
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    complement(11587..11708)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(11791..11809)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11830..11846)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    11854..11870
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11878..11908)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(11923..11944)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(12232..12820)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(12994..13851)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(13852..13956)
                     /label="AmpR promoter"
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttg ccattcgcca ttcaggctgc
      481 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
      541 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
      601 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgacccg gggatggcgc
      661 gccagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      721 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      781 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      841 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      901 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      961 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatgctga
     1021 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     1081 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     1141 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     1201 aggtctatat aagcagagct ggtttagtga accggggtct ctctggttag accagatctg
     1261 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     1321 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     1381 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     1441 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     1501 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     1561 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     1621 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     1681 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     1741 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1801 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1861 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1921 caagccgccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1981 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     2041 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     2101 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     2161 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     2221 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     2281 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     2341 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     2401 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     2461 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     2521 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     2581 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     2641 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     2701 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     2761 agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa
     2821 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2881 acaaactaaa gaattacaaa aacaaattac aaaattcaaa attttatcgc gatcgcggct
     2941 cccgatcgtt gcgttacaca cacaattact gctgatcgag tgtagccaga tctaagtact
     3001 aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc
     3061 cagtgcaaat gcaggtgcca gaacatttct ctgtcgacga attctcttaa ttagaagagc
     3121 aatgtctgta cttagattgt gaacaactta gaaatgggcc ccgatttttc ctccacatct
     3181 ccaatccaag tcacactggc tgtcctcaga tgtaagcatg taggcaattt gaggataagg
     3241 acaaagagta tgtgcaatga gaaagattga catttgaatc accgtattga ttatagtagg
     3301 attgtttgta atgttattag tcatgatctt tcatcaatca atcttttaaa aacaggtata
     3361 ttatgagtct tttgttaagc tctacattta ggagagaaaa gagacaatgg tgtaaagcac
     3421 ctttagtata ttgatcaatt agaagtatct cttctagtag acggattatt aaaaagatgt
     3481 tatcactatc cctgagaaga ttctgtaaaa ctggatctga gtcaattatt gcaattactt
     3541 ctcaaccaca gctggaagac ttgcaggaga atgacacgtg tcacaatcct gtgtgattac
     3601 catggtgagg tctcctggag ccaccgggtc caacccaatg tactttggca aaagaacagg
     3661 gatgattggt tgtccatttt tggaagtcac cttcttaccg gtcagcttgt ggaccagagt
     3721 ttctggcact tcgataccca tgatattttt ggttggatca attggaacga tcttaaagtc
     3781 ctggagtgaa gtcattattg cttggatttt ctccggagat gttagatcgg cactgttccc
     3841 cttcttccca cttttgttgg gtaaaagaat ggggcgaagt tttggatgaa atgaaattcc
     3901 tggacgcaac gctccatttg atccagttgg agtgtcatcg gtccatgttg cagcaggcag
     3961 tggttgagtg atcagtttga gtgctgtcaa atcaaaggtg aaatactggg gtagttggac
     4021 tggtggaaga acgaactcct ggaggaaagc ctggtttcca attcgcagga gcctgagggg
     4081 gtgatccggg attccaggac ccagccgatt gactctgaca agcggattgg ttgccttgcc
     4141 gaaatgggtg atagtatatg aagcaagcat gatggcggcc gtagttgagt caaagctgta
     4201 ggtcttttga tcagcgacac ctagaggaag ccaaattgga atttgcttca ttagcacttt
     4261 ggggcccgat atgacattca ccatagcttc gaggatgaat gctgatgaca cactgcctgg
     4321 tgtgtggctg gcatggtcga tggtgtcatc agcaattggc ctgagtggat tcgatggagt
     4381 gtctccattg actgactccg gtgtcaggaa gcctgtattg ctgttgccac ccctagcaat
     4441 tgttgaattt gacctggcag ggtatatggc ctccatatat tcaggaggag cagtaggcaa
     4501 tataacccgc ctcatatttt taacaatttt gctcaacgtt tacaagatga aggttaatga
     4561 aaaaacactc tctcagtcga ggtaggtttt tcttaatctt catcgcggcc gcgagaccat
     4621 ctggaaataa actcagaagc cctgtcagcc tttcgataag cttaacttca gaatcctgca
     4681 ttctatgtaa atataaggtt tgaactaaga aacgttcttc acaattacaa tctctaatat
     4741 gatagaacct attgcacaca ctaatcaact ctggtaattt cttaaactca gcttgccatg
     4801 tcccattatg ttttaatgct tgtacaataa gaaagaattt taaataatca ttgactagtt
     4861 ttgtgattcg tccttttgca gtacgaataa agtgatatgt ttgagtccga ctttgtcgct
     4921 gttgattata atcattggtc aattctcgaa tctctgccct aagatgtgct aagtgctgag
     4981 tgacagagac tagtggacct ctttttgaat cgacaaggtt atacctgtgg tatagtactt
     5041 tctctaatga tggaaaacca aggcggatat agcttaaatg taaatcgcag tcagcatact
     5101 gagttaaatg acttagccag tatgggctcc gttgaatttg cagttgcaat gccgtaagta
     5161 ttacctgctt tcaactgaga tggttttggt gtggcatctt acgtgtagtt gataagaagt
     5221 tcgtcagaca aagataccac tcactagacc tggcacttga cgttattggc ttaattaaat
     5281 acccagtggc aaaaaacggg gctagattat catttagcca taacataccc tcggtatcac
     5341 ttagaaagac tttaaggacc actgctttca atacgctggg atcaacatgg tgtaagatca
     5401 atttatgtac agcttcgtac aatttcgatc tgtttatatt ctctgtcgtc tctgcatcca
     5461 tggttataac ctcgacttgc ctaggtagcc ttgctctttg gtctttaaac caagtaggat
     5521 ttgttatgtc tgttatttgg cttgctgagt tgttaagaat aatctcaatt tggtcattat
     5581 ggaattttga cataacaggt agaagcatcc taggagtagt cattcctgat actatttctg
     5641 actctatact ggactcagta gctagcgtgt tgaaaaataa ggtcttaact tggtatttct
     5701 gaatcaatag taaggcacca gcaccttctc cctctgccag cgtcacagcc gacttaaaat
     5761 tctctatttc ctaaaggacc tcatcaagtt tgtaatgcat ggacgagact ataccggtaa
     5821 acctacaata aactgtggta tcaatgacgg atctcaattg ccgtaagtac tttgatatct
     5881 catcttgggt ttgtgactca ttggatgacg ttaattggcg tgtcccttga gataatgtaa
     5941 agaaaggtag gactagacga tgtgagatta tttgatgacc ttccctcttg gatgctgagt
     6001 tatgatcctg agatttcaca ttgtgtctcg atctatcgaa atttagtttt gggtttgctg
     6061 taccgcaagc agagtcactt agaaatgact ggaacgacgg accctggtgc gtgttctctt
     6121 gtggaattgt cgttcttttt atttcatggc tcatttgcag gactagactc cgttcagtgc
     6181 catcatgtgg atctctggtg gtttgttctt gacttctctt aatatgacca tcactgttgt
     6241 ttgtgcttga tccagttgaa gagtttcttg tcaagtcttt tcggttgctg tttctgtgcc
     6301 tgctcctcca gtacgccaat gacgcatgga agaaattgct ggctgtactc ttacatgtgt
     6361 aaacaagatt gtcgtgagga tgtacatgat catttatggt agttttaaaa gcctggtgtc
     6421 ttgatgaatc atgcaccagc agttctacga tctgatggag gaaattatta acaggtgttg
     6481 gattttgacc ctcaaatgga tatactatcc ataaagggac aattgttccg cgattaatta
     6541 tccattcctt tacaaatgta agaaaagatg aaatggacgt tctcaaaaat aacctggccg
     6601 catctgagag tcctctgtca cctgcagcac cgcctatgta aatagaaaaa tggggatcaa
     6661 tactcattaa tcgtgacata acgcttgcat gtttgaatgt tggcttaagt attcgcaatg
     6721 agcgatgtgg tagattatga atttgggtag ttaagtaata taaaaaattg tcaagtgtta
     6781 atttcttagt ccgaagaatt gtattgccaa gataataact tacaaaggcc ccaaaactgt
     6841 acagaagtcc tatcttggga taagttaaga aatgggtagt gaatgatctt gtttctccac
     6901 tgctaattgg gtctgtagac gaattactcg agacgaatta ttgctatctg aaataattga
     6961 ttgcatgatg gtcttagcta gctcccatcc agataagtga ggcagtcgaa taaggtcatc
     7021 ttctataatg ttcagctgtt tgccttggaa aaatgggtta tcaaatgaga tattgcaatt
     7081 gagtcctcct tttagaggat tattgtcata aatcaattca ttttctcggt atcttgttaa
     7141 atctaaatcc aatgtagatg tgtatgttaa gtactgagct ggtacctccc gggtgcaaca
     7201 cttagttaga tgaaggtgag cacgattata ctggatatct gtagcctcag tgtttctaaa
     7261 tttaatatcg aacagtgcaa ctgcataatt tataacattc tggaaaataa tattgctgtc
     7321 gcgtgccgat tggccacctc ctgaaaactc acctaaagtg tttgtagaaa caatcaatcg
     7381 cgttgctgag ttactcatac gattggccat gaaagaatga ggactgtatt gatcgttgta
     7441 cctatgaaca atatttcccg agtaatgtga aggggtcatt tgaagtattt cttgaacact
     7501 taaatttact cgtgcttcca aaaatggttt tattagcaag tcactgttcg aactgccttg
     7561 agttacccat gttaaacggg acgccaattc aatggcctct cttaaggctg cggaaggaca
     7621 ttttggttta atagcaggtt gccctatctt atcttctgtc cttgatccaa tgtatgggat
     7681 tccatccccg atagtccagc ttattcggga tgcatttggc catgcactaa caatatgttt
     7741 cttgactgct actgacacga atggtttccc accaggttgc ttggcatttg aacactgcgg
     7801 acattgttcg tagggtttca gccaaaccac tttgaattgc tcaatcatac atgggagtgt
     7861 ggctccaata agaggtctcc cctctaaaat atgtgcccat gaatattccc tcaggatctg
     7921 tgctaaatca actgtgcaag ttatttgggt taaagcctcc gccaggatat tatcacaatg
     7981 atcaagataa ctaaaccata gactccacct ttgcaatgtt atcttcctca gtctgtccaa
     8041 aaccggcgtc tctgtattat tgttgatgat cttagaggct aataatgtgc gtgttccttc
     8101 caagtatcct agaatttgca atcgcttccc gctcggcgtg cgtgaaaata tatcggctgc
     8161 gaaacgactc ataacaggag ttgatgataa gagccactta caaaccattt cgtcttcgaa
     8221 gtcagctgat gcatgaaata aggtattaat aagtttgttt ttcgcactta gggtgatagt
     8281 cctacgtaca atctggcgca gaaatgaagt taagtcttgc gacccaggaa catttaatcc
     8341 gctaggattt agcacaaagt caatggcagt gcagttccca gggttcttcg caattaaagg
     8401 taagaataaa tcatccatct caatcattcg gagataagtt tttaactgga ataaacctga
     8461 ggtaactgga tctcctagat tccggtagaa acatttctca ggattcaaga aggataaccc
     8521 tccaagcacc tgcggtaccg ctagtgccaa tgatattgtt ccgaaatcca gaggtttgcc
     8581 gagtgttaac tgtccaaggt caaaaccttt attaaatccg aggtgatgat attgcaagat
     8641 tctcaccgaa aagaacgtat ggaaagctgc ggttattctg caaggaaaga tatgtcgtgt
     8701 ctcagagatg gatcgctcaa aagcactacc tatactagcc agggtccctt gaagatcatc
     8761 aaaaattgca tcagacaatg gtgccattct tgtagccgtt ttaagggact gaggcaattg
     8821 gaccccattc aaatattgtt tttttccaaa atagataaaa cctgaatgta caaatgtttc
     8881 atcaggtttt aaaaagattc cacaggcact tgtaactttt gctaggctgg cggccaccct
     8941 cgctgcattg tcctcggcgc tctgttcctg ctcgcctgca tcagtctcta aggggaagac
     9001 tgataaaacg gtaatgcact gattgtcacc catcacagct gagcgcaact taaaaccagt
     9061 cttaatttca actaaagaaa tttgagcaca tgaaatactt gtccagagtt tttgttgcag
     9121 tccttcaatc cctcccatat gacccctgta tgaactaggc ccttcagggg ggttgtttcg
     9181 attttccagt gtgaggttat gcggtggatt ataataatca ctgacatgca tataacactg
     9241 tgggattgta taatgcatcc aattaaaaac attcttaaca ccatagcaac ggttgcaata
     9301 ttctataaaa ggtgctgtaa actcatacct aaatgcaaga ttgtatttct ctaaatcagt
     9361 tacaaagcta ctccctctaa ctgtggcatg ctcaccgaaa tcatcacttg tgtggtgcca
     9421 tgatgcttga tgcaataagc tttctttttg ttcacgttcc gtaactacca tcatattgct
     9481 aggaaatgct ttagcaagac catcagctaa cagagcttca caaagtgttt gaacattgcg
     9541 agtcggataa ggcaattttc cgaaagttct acctacattc aactctttct ctttcaatga
     9601 gaaagaaaaa ttccgatatt gtggtagtag atactcaagt ttttgcgcgt aggaaagaac
     9661 attctcaata gaaaagtttt cttgctctaa aaattgttcc ggtacacgtt tggtactgaa
     9721 tttgtgaggt ggattatatc ccagaacatt aggctcgaat actgcatccc agcatgtcct
     9781 ttctactgca gtagctctgt cttttataaa aatacttaag tcactaataa ttttggttga
     9841 gaaaagtgga ggatggtcaa ggtggtaaaa ttcccatagc agttctttaa tcattggcaa
     9901 cggagggaat tgatttcttt tgatataaga attaagacct ggtgttagat ttctatctga
     9961 ggtaacactg taccaagatc cttgactatc aaaataatgt tttgcaatgc tatatttaaa
    10021 aacacaatat gtctcgaaaa tcacgatagg gcgtaatgct tttagcaccg tagcatgttt
    10081 tttaactttt tggattgctg tttcactatg tagcacagga tgcccccagt gtttttgtat
    10141 ggaaaatagc tcacaaagtt gttgtggcgt catctccagc cttatcaatg ttctatggaa
    10201 ttcacggatc ttgtgagcct gtgaaggctt tagtgcacgt atttctgtaa tttcttccag
    10261 ggtgtgattt acagctaaat gcatttgtgt taagaatcgg cccttcctct cggtgtactt
    10321 tgagcacaat tgaattttag ccaagcacaa tggttcgaga aacttaatga ttttataccc
    10381 atcagaccct aatatggaga gtaagtaatc tccgctctgg taaagcatag acacaatctt
    10441 aaaattggga taatcagagc aaactgggtc ctcaacctct gctatttttg agattagagt
    10501 tgtgttgaat cgacatgtaa ttaaatcttt gcacattatc aagacatcgg cagtagaaac
    10561 agaaacaatg tgtgtatgcc cttgaaccgc ttctttgaat actgatgtct gataccaatc
    10621 catagcagca tgggggattc cttgtgtgtt cagtggtaac agtgaaattg ggatcttcca
    10681 aaaaacatag tccccatagc ctaagatgtc tattaaatca tcatgaacaa accacgttga
    10741 tctagagttt cctcgattta atctacccct tcgagttaaa atagccaggt cataccagaa
    10801 aaacatttga tgtaaaaatt cattgccctg aattgaagat aagatttttt cttgaaagtg
    10861 gtcatcaaca cagtcttctt gagcacccac atttttgaga taatatttca ggaagagagc
    10921 atcttgcatt gtgtacttaa taatttcatc taagaactgt tggcaccgcg gctcaacagg
    10981 acagaaccca ttgcctgata gtgccttgag aagaattggg actatgaaat ctatgggcaa
    11041 tgccgccact ggtacatcac ttaagaactt ggtaacagtt acatcatatt ttaaacggta
    11101 tatatgtttc gggagtttac agttgcgtag ttgcggatta agggagtatg atgaatacaa
    11161 cccgcaagct ctagtgacaa ggtcacattg gtccaataca attggtgatg ataacctggc
    11221 gtctgggtat tgggtggtaa ccttgggcta tcgaaactta attaaagtac ctttaagacc
    11281 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
    11341 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
    11401 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
    11461 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
    11521 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca ggcatgccag
    11581 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
    11641 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
    11701 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
    11761 aggttttttg gcgcgccatc gtcgaggttc cctttagtga gggttaattg cgagcttggc
    11821 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
    11881 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
    11941 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
    12001 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
    12061 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
    12121 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
    12181 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
    12241 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    12301 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    12361 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    12421 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    12481 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    12541 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    12601 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    12661 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    12721 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    12781 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    12841 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
    12901 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
    12961 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
    13021 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
    13081 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
    13141 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
    13201 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    13261 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    13321 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    13381 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    13441 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    13501 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    13561 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    13621 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    13681 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    13741 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    13801 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    13861 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    13921 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccac
//
