LOCUS       V003280                11028 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003280
VERSION     V003280
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11028)
  AUTHORS   Mattiuzzo G, Ashall J, Doris KS, MacLellan-Gibson K, Nicolson C,
            Wilkinson DE, Harvey R, Almond N, Anderson R, Efstathiou S, Minor
            PD, Page M.
  TITLE     Development of Lentivirus-Based Reference Materials for Ebola Virus
            Nucleic Acid Amplification Technology-Based Assays
  JOURNAL   PLoS ONE 10 (11), E0142751 (2015)
   PUBMED   26562415
REFERENCE   2  (bases 1 to 11028)
  AUTHORS   Mattiuzzo G, Mitchell J, Mee ET.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUN-2015) Virology, National Institute for Biological
            Standards and Controls, Blanche Lane, South Mimms-Potters Bar EN6
            3QG, United Kingdom
REFERENCE   3  (bases 1 to 11028)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11028)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Geneious v. 7.1.8
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2015"; volume: "10"; issue: "11"; pages: "E0142751"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (19-JUN-2015) Virology, National Institute for Biological Standards
            and Controls, Blanche Lane, South Mimms-Potters Bar EN6 3QG, United
            Kingdom"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11028
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     601..617
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        624..642
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        713..1016
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1017..1220
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             1235..1415
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1462..1587
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    2080..2313
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             2498..2542
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2691..2732
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2809..2925
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     misc_feature    3001..3092
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     gene            complement(3097..5280)
                     /gene="NP"
                     /label="NP"
     gene            complement(5284..6153)
                     /gene="VP35"
                     /label="VP35"
     CDS             complement(7208..8284)
                     /gene="GP"
                     /label="Pre-small/secreted glycoprotein"
                     /note="Pre-small/secreted glycoprotein from Zaire
                     ebolavirus (strain Kikwit-95). Accession#: P60171"
     LTR             8386..8619
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    complement(8636..8757)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(8840..8858)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8879..8895)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    8903..8919
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8927..8957)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8972..8993)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9281..9869)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(10043..10900)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10901..11005)
                     /label="AmpR promoter"
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttg ccattcgcca ttcaggctgc
      481 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
      541 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
      601 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgacccg gggatggcgc
      661 gccagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      721 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      781 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      841 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      901 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      961 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatgctga
     1021 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     1081 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     1141 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     1201 aggtctatat aagcagagct ggtttagtga accggggtct ctctggttag accagatctg
     1261 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     1321 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     1381 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     1441 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     1501 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     1561 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     1621 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     1681 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     1741 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1801 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1861 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1921 caagccgccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1981 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     2041 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     2101 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     2161 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     2221 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     2281 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     2341 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     2401 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     2461 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     2521 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     2581 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     2641 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     2701 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     2761 agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa
     2821 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2881 acaaactaaa gaattacaaa aacaaattac aaaattcaaa attttatcgc gatcgcggct
     2941 cccgatcgtt gcgttacaca cacaattact gctgatcgag tgtagccaga tctaagtact
     3001 aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc
     3061 cagtgcaaat gcaggtgcca gaacatttct ctgtcgacga ttgccatgaa tttattcctg
     3121 tgattcatta ctggccaata aaattgttga ccatccagtg taacaaatct attctcatca
     3181 ttcatggcct ctttttcagt gagccatggt ggatattcct cttcaaggga gtccggatac
     3241 gtgtactctt taccatcact ggtactgaaa actacaggct catccttcat catatgataa
     3301 tacaaaacgg catcaaatgg cccctgtgat cttagaatgt ggcgatacat ctcctcaaaa
     3361 gaatgttctg gctgggtgtt gtcactgtct tggttcctgg cctcttgaat gtggtcctga
     3421 tcttgttgtt catcttgcgg gagttctttc ttttcggagt gatctctgta tacgggagcc
     3481 ggtggggcga cagtgggtgt gcggttagaa gttccgtccc tgtcctgttc ttcatcatct
     3541 gactctaagg gcggaaggct agacgtctcg tcgtcggcat cgtccagtgg gtctgcctct
     3601 tcgttgattg gggtcagcat gcgagggctg gttgagccgg agggttcgtt tcttctgtca
     3661 ttgtccgtga gtggagcact ggcatggtgg attgttctgc gagggcctgt gacgttttga
     3721 gttggcgtgg attgtgtctg tctgccctct gtatgctggc ccttttgact gtttttctgt
     3781 tgtccaccct tggtcgatct gttaggcact ggcttggtgt cctcgtcgtc ctcgtctaga
     3841 tcgaatagga ccaagtcatc tggtgcactc atgccgtttt ccgagtaact ttggtattcg
     3901 ccttagcctc catcatcggg atcaactacc acatcgggaa tggtcgtatc ctgtgagtca
     3961 gtcggatcat catcttgatg gccaggattg tcgtcatcat tgatgggtcc tggaaaggga
     4021 atgtcgtcat catcatcgta atgtccactt gttttgggca gtgatgcagc agtgatagct
     4081 tctgtcagct tggccaggcg ctcttttctt agagttacca tcgcgtttgt ttgctggaag
     4141 ctgatttcgt tctttttctg atggaagttc ataagaattt tcttttcctg atcatcaagt
     4201 ccaagatggt caagttcacg agactccgca tattgttgga gttgcttctc agcctcagtg
     4261 gctgcctctc tgagctgttg atactgttct ccaacattta ctcctgcgag ggtgctcccg
     4321 tgggctgtgg cgactccgag tgcaattgcc gacagttgag ggaaaagacc atgctcaaga
     4381 ttatttactc cagaaaggtt caaaagtcgg gcgaaaggag catactctcc atgcttggcc
     4441 agggagctga gtgcagcctt gaaggagttc acctcatttt ttaccttggc ggtccttgca
     4501 agaggatgga gacgaactcc tcgttctgtc ttttgtagga tatgatcaag tactgttttg
     4561 acaatcaata gacctgaaaa acgagcttga gccactgaat ttgaaatcac agcatcgttg
     4621 gcatcatgtc cggcaaccat gtgcatccct tggtgtatta gaagaaattt gatcaaaaaa
     4681 tttgttcgca tcaaacggaa aatcaccatc atgtgtccta ctgattgcca agctgttgga
     4741 tattatatca gtccttgctc tgcatgtact tgaatttgcc tttgaacctt ctcaaggcaa
     4801 gccttttctc ctactaccaa tttcggaagg aatagacttg caaaggagag gaactgaccg
     4861 gcattagctt cagtcgtctc ctcttccggc atggcagcaa gtgttctctt aatgtttctc
     4921 ccactagata ctgctggcag caattcctca aggcgcttca ctccatcacg cttcttgact
     4981 tcaaaacgga acccgtgccc ttccaaatac ttgactgcgc cactttccaa gaaaagtttg
     5041 taatctcctt ggtacgcatg atgaagacaa agcatgagaa ggaaactgtc cgcactctct
     5101 tgaaaatcaa caccagcttc aaaggcctgt atgataagtt ggcaaatttc ctcaagattg
     5161 tttacttgat acactgggat gactctttgc cgaacaatcc cctgttgaac ggacagacct
     5221 gctgtcaaga tcttgtggta atccatgtca gattcagtga gactcggcgt catccagacg
     5281 aattctccaa gtgttttacc atcttgaagc tgaaaaacac atacccaacc tcgatcaatc
     5341 ttgggtgatg gtgggactgg acgcaagctc ttctggcaag ctcggggaat gtcacctcga
     5401 gagcggatgt ggatgacagg tggagcagca tcttggaaga ttggaactct ttttgtaatt
     5461 tgaattaggg cacattgagg ggagtctcct tcagccaggc tggcctggaa ctcagcatga
     5521 ataatgtcca atgaattgct atcttttccc aatttacaaa tcacttgtac taattggtgg
     5581 aaagcagttc caaaaccagg caagtgatca tacataatgt ttctcaaatc ctttgcctaa
     5641 atgtcaggtt tcccaaaatt ttcctcagtt agtgaagtgg tactgtctag attgttgaat
     5701 gcctccctaa cactttgagg gacagtctca tctctagatt caatcttacc ccgaatcgca
     5761 ctttcttcat aaagtgatgg tccaggtggt ggttgaccat gttcagccca ataagcctca
     5821 gttgccgcag cggttgctgt tgcccgaccg gttgtcatca ccagaagatc atattttgca
     5881 accatctcag cacaaaccct gttcaatgag gagattgttt ttgccatatc ataaactggc
     5941 tttagaccat tctcaagact cgtaatgcgt tgttctaatg attctgatgc gatggtttgt
     6001 tattgcacaa cagtagccaa tgaagccaat gtttgtacta cctcctcaaa actatgattg
     6061 caaattgggt ccgtttgggt ttgactgttg cgcatcttcg ggtttggctt cgtttgttgc
     6121 atttgggatg cgtagcataa tcctggattg ttctcaatat cacagaagat gtcgtttaca
     6181 ggaatccttc cggtcattag ctgctcagag atccagcccg aaagctcagg gcctggcatt
     6241 ctgtcgtttt gagtcgtggc cacagtatgg cccctgccgc tagcgcatat acagaataaa
     6301 gcgataactg caattataac acctgtaact ccaatacctg ccggtatcca ttgtctccat
     6361 cctgtccacc aattgtcatt gtccccctgg tccggaaggg ttttatcaac aaaatcatga
     6421 ataatctgat caattttgtc tgttatgttc ttggtccaat catgtggttc gatacagcag
     6481 tccggtccca aaatgtggca tgtgccaccc catcgctgca gcaggaagtc aattgcctta
     6541 cggttgagga ttgaaaaggt tcgcagctca gttgtggctc tcaggaacag ttggagagct
     6601 tgagtcgttt cgttggccag ctgcctcaac ccacagatta aaccatcttg gttgtgcatt
     6661 agcccctctg tgtaaattcc ttcggctgct ggcccgaaat atggtatcca ggccaatccg
     6721 attgcagcac cttcatcctg agtagtccag taatgtaaat tggggttgca tttgggttga
     6781 gcattgacaa ttacttctct tcgagtcctt ctcccgcctg tgatcagtcc tgctactcca
     6841 gcaatagtat tggtaattaa gcctagcttc ccgctgctgg cactctcttc tccggtatct
     6901 tggtgatgag tgttgttgtt gccagcagtc tcgctgtagt tttgggggct tgtcgtggtg
     6961 gcgaggtcca gggagtcagc gctcttactc gtgttggtgt tctctgcttt taagggtccg
     7021 gctgcggtcg tggcgggggg agtgtcggag gctgtgctgt cgttgtctgc tcaacggtga
     7081 tgttgtccaa cttgagttgc ctcagagatg tcaagtttat acacgggtgt attatgggtg
     7141 ctgttgtccg gacctgtttt ggttgtggga ggttgaggac tcgtggagat tgtggcaagg
     7201 gttgtcagat gcgacactgc agctttcctt ccttgactgt gcacttgaac cattgcagag
     7261 gaattttctg aagccatgat tttgtggtct tcatttgttg tgttggtctc tgggtcggaa
     7321 gaagttcgcg ccggactctg accactgatg tttttgggtc cgtttgatac agctgtgaaa
     7381 gacaactctt cactgcgaat ttttctagtg aggttttttt agtttcccag aaggcccact
     7441 ccccgattgt tgtatcaatt tcggggttga ccttccaaat tagttttccc gtggtgttgc
     7501 tcctcttccc acttgcatat attgtctcat tcagctggag cagaaactgt ggtgtgaatc
     7561 ttgattcaag ttggacgtag gtcaaattgt caacctcgaa caagtactct gtctcattag
     7621 ttccaaaacc ggtagcctga tatctaattg tggtagaata atagccactc gacgggtcct
     7681 ccgttgcatt gaccggctct ctcaaggggt gtgagctgaa gaagtccttc ttagcttggg
     7741 gcagtatcag aaatgcaacg acaccttcag cgaaagtcgt tcctcggtag ataactgtgg
     7801 aagcaagtcg atcatacagg aagaaagcac cctctttgtg gaaggcaaag tctccggcac
     7861 atggtcccgt tcctgatact ttgtgcacat accggcaccg ggggaagccc cgaatcccgt
     7921 ctggcgctgc tggtagacac tcactcccgt caggtttttt gatttcaaga ttgtagcagt
     7981 tttcagccca ttcaccagct tcataattga ccacctttgg tgggacaccg gacctgaagc
     8041 cccatctttt agtcgcagat ggcacgtcag ttgccactcc attcccctcg agattcagtc
     8101 caactgatct caattgattt gtggatgaca gtttgtcacg acaaactagt ttgtcgacat
     8161 cactaacctg taatgtacta ttgtggataa ctccaagcgg gatggaaaat gttctttgga
     8221 aaaggataat tacccaaaga aagaatgatg tcctcttgaa tcgatcacga ggtaactgca
     8281 atatggtaac cttgggctat cgaaacttaa ttaaagtacc tttaagacca atgacttaca
     8341 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     8401 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     8461 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     8521 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8581 ccctcagacc cttttagtca gtgtggaaaa tctctagcag gcatgccaga catgataaga
     8641 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     8701 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     8761 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttgg
     8821 cgcgccatcg tcgaggttcc ctttagtgag ggttaattgc gagcttggcg taatcatggt
     8881 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     8941 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     9001 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     9061 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     9121 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     9181 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     9241 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     9301 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     9361 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     9421 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     9481 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     9541 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     9601 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     9661 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     9721 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     9781 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     9841 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     9901 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     9961 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    10021 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    10081 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    10141 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    10201 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    10261 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    10321 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    10381 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    10441 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    10501 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    10561 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    10621 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    10681 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    10741 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    10801 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    10861 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    10921 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    10981 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
