LOCUS       40924_39822       14110 bp DNA     circular SYN 13-MAY-2021
DEFINITION  This is an expression plasmid for production of recombinant genomic 
            SFV RNA encoding beta-galactosidase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14110)
  AUTHORS   Liljestrom P, Garoff H
  TITLE     A new generation of animal cell expression vectors based on the 
            Semliki Forest virus replicon.
  JOURNAL   Biotechnology (N Y). 1991 Dec;9(12):1356-61.
  PUBMED    1370252
REFERENCE   2  (bases 1 to 14110)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 14110)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechnology (N Y)."; date: "1991-12"; volume: "9(12)"; pages: 
            "1356-61"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             7430..10474
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(11409..11426)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11580..12168)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12342..13199)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(13200..13304)
                     /label=AmpR promoter
     primer_bind     13372..13390
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
     primer_bind     complement(13428..13450)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     13550..13569
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     primer_bind     13784..13803
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI,
                     reverse primer"
ORIGIN
        1 gatggcggat gtgtgacata cacgacgcca aaagattttg ttccagctcc tgccacctcc
       61 gctacgcgag agattaacca cccacgatgg ccgccaaagt gcatgttgat attgaggctg
      121 acagcccatt catcaagtct ttgcagaagg catttccgtc gttcgaggtg gagtcattgc
      181 aggtcacacc aaatgaccat gcaaatgcca gagcattttc gcacctggct accaaattga
      241 tcgagcagga gactgacaaa gacacactca tcttggatat cggcagtgcg ccttccagga
      301 gaatgatgtc tacgcacaaa taccactgcg tatgccctat gcgcagcgca gaagaccccg
      361 aaaggctcga tagctacgca aagaaactgg cagcggcctc cgggaaggtg ctggatagag
      421 agatcgcagg aaaaatcacc gacctgcaga ccgtcatggc tacgccagac gctgaatctc
      481 ctaccttttg cctgcataca gacgtcacgt gtcgtacggc agccgaagtg gccgtatacc
      541 aggacgtgta tgctgtacat gcaccaacat cgctgtacca tcaggcgatg aaaggtgtca
      601 gaacggcgta ttggattggg tttgacacca ccccgtttat gtttgacgcg ctagcaggcg
      661 cgtatccaac ctacgccaca aactgggccg acgagcaggt gttacaggcc aggaacatag
      721 gactgtgtgc agcatccttg actgagggaa gactcggcaa actgtccatt ctccgcaaga
      781 agcaattgaa accttgcgac acagtcatgt tctcggtagg atctacattg tacactgaga
      841 gcagaaagct actgaggagc tggcacttac cctccgtatt ccacctgaaa ggtaaacaat
      901 cctttacctg taggtgcgat accatcgtat catgtgaagg gtacgtagtt aagaaaatca
      961 ctatgtgccc cggcctgtac ggtaaaacgg tagggtacgc cgtgacgtat cacgcggagg
     1021 gattcctagt gtgcaagacc acagacactg tcaaaggaga aagagtctca ttccctgtat
     1081 gcacctacgt cccctcaacc atctgtgatc aaatgactgg catactagcg accgacgtca
     1141 caccggagga cgcacagaag ttgttagtgg gattgaatca gaggatagtt gtgaacggaa
     1201 gaacacagcg aaacactaac acgatgaaga actatctgct tccgattgtg gccgtcgcat
     1261 ttagcaagtg ggcgagggaa tacaaggcag accttgatga tgaaaaacct ctgggtgtcc
     1321 gagagaggtc acttacttgc tgctgcttgt gggcatttaa aacgaggaag atgcacacca
     1381 tgtacaagaa accagacacc cagacaatag tgaaggtgcc ttcagagttt aactcgttcg
     1441 tcatcccgag cctatggtct acaggcctcg caatcccagt cagatcacgc attaagatgc
     1501 ttttggccaa gaagaccaag cgagagttaa tacctgttct cgacgcgtcg tcagccaggg
     1561 atgctgaaca agaggagaag gagaggttgg aggccgagct gactagagaa gccttaccac
     1621 ccctcgtccc catcgcgccg gcggagacgg gagtcgtcga cgtcgacgtt gaagaactag
     1681 agtatcacgc aggtgcaggg gtcgtggaaa cacctcgcag cgcgttgaaa gtcaccgcac
     1741 agccgaacga cgtactacta ggaaattacg tagttctgtc cccgcagacc gtgctcaaga
     1801 gctccaagtt ggcccccgtg caccctctag cagagcaggt gaaaataata acacataacg
     1861 ggagggccgg cggttaccag gtcgacggat atgacggcag ggtcctacta ccatgtggat
     1921 cggccattcc ggtccctgag tttcaagctt tgagcgagag cgccactatg gtgtacaacg
     1981 aaagggagtt cgtcaacagg aaactatacc atattgccgt tcacggaccg tcgctgaaca
     2041 ccgacgagga gaactacgag aaagtcagag ctgaaagaac tgacgccgag tacgtgttcg
     2101 acgtagataa aaaatgctgc gtcaagagag aggaagcgtc gggtttggtg ttggtgggag
     2161 agctaaccaa ccccccgttc catgaattcg cctacgaagg gctgaagatc aggccgtcgg
     2221 caccatataa gactacagta gtaggagtct ttggggttcc gggatcaggc aagtctgcta
     2281 ttattaagag cctcgtgacc aaacacgatc tggtcaccag cggcaagaag gagaactgcc
     2341 aggaaatagt taacgacgtg aagaagcacc gcgggaaggg gacaagtagg gaaaacagtg
     2401 actccatcct gctaaacggg tgtcgtcgtg ccgtggacat cctatatgtg gacgaggctt
     2461 tcgcttgcca ttccggtact ctgctggccc taattgctct tgttaaacct cggagcaaag
     2521 tggtgttatg cggagacccc aagcaatgcg gattcttcaa tatgatgcag cttaaggtga
     2581 acttcaacca caacatctgc actgaagtat gtcataaaag tatatccaga cgttgcacgc
     2641 gtccagtcac ggccatcgtg tctacgttgc actacggagg caagatgcgc acgaccaacc
     2701 cgtgcaacaa acccataatc atagacacca caggacagac caagcccaag ccaggagaca
     2761 tcgtgttaac atgcttccga ggctgggcaa agcagctgca gttggactac cgtggacacg
     2821 aagtcatgac agcagcagca tctcagggcc tcacccgcaa aggggtatac gccgtaaggc
     2881 agaaggtgaa tgaaaatccc ttgtatgccc ctgcgtcgga gcacgtgaat gtactgctga
     2941 cgcgcactga ggataggctg gtgtggaaaa cgctggccgg cgatccctgg attaaggtcc
     3001 tatcaaacat tccacagggt aactttacgg ccacattgga agaatggcaa gaagaacacg
     3061 acaaaataat gaaggtgatt gaaggaccgg ctgcgcctgt ggacgcgttc cagaacaaag
     3121 cgaacgtgtg ttgggcgaaa agcctggtgc ctgtcctgga cactgccgga atcagattga
     3181 cagcagagga gtggagcacc ataattacag catttaagga ggacagagct tactctccag
     3241 tggtggcctt gaatgaaatt tgcaccaagt actatggagt tgacctggac agtggcctgt
     3301 tttctgcccc gaaggtgtcc ctgtattacg agaacaacca ctgggataac agacctggtg
     3361 gaaggatgta tggattcaat gccgcaacag ctgccaggct ggaagctaga cataccttcc
     3421 tgaaggggca gtggcatacg ggcaagcagg cagttatcgc agaaagaaaa atccaaccgc
     3481 tttctgtgct ggacaatgta attcctatca accgcaggct gccgcacgcc ctggtggctg
     3541 agtacaagac ggttaaaggc agtagggttg agtggctggt caataaagta agagggtacc
     3601 acgtcctgct ggtgagtgag tacaacctgg ctttgcctcg acgcagggtc acttggttgt
     3661 caccgctgaa tgtcacaggc gccgataggt gctacgacct aagtttagga ctgccggctg
     3721 acgccggcag gttcgacttg gtctttgtga acattcacac ggaattcaga atccaccact
     3781 accagcagtg tgtcgaccac gccatgaagc tgcagatgct tgggggagat gcgctacgac
     3841 tgctaaaacc cggcggcatc ttgatgagag cttacggata cgccgataaa atcagcgaag
     3901 ccgttgtttc ctccttaagc agaaagttct cgtctgcaag agtgttgcgc ccggattgtg
     3961 tcaccagcaa tacagaagtg ttcttgctgt tctccaactt tgacaacgga aagagaccct
     4021 ctacgctaca ccagatgaat accaagctga gtgccgtgta tgccggagaa gccatgcaca
     4081 cggccgggtg tgcaccatcc tacagagtta agagagcaga catagccacg tgcacagaag
     4141 cggctgtggt taacgcagct aacgcccgtg gaactgtagg ggatggcgta tgcagggccg
     4201 tggcgaagaa atggccgtca gcctttaagg gagcagcaac accagtgggc acaattaaaa
     4261 cagtcatgtg cggctcgtac cccgtcatcc acgctgtagc gcctaatttc tctgccacga
     4321 ctgaagcgga aggggaccgc gaattggccg ctgtctaccg ggcagtggcc gccgaagtaa
     4381 acagactgtc actgagcagc gtagccatcc cgctgctgtc cacaggagtg ttcagcggcg
     4441 gaagagatag gctgcagcaa tccctcaacc atctattcac agcaatggac gccacggacg
     4501 ctgacgtgac catctactgc agagacaaaa gttgggagaa gaaaatccag gaagccattg
     4561 acatgaggac ggctgtggag ttgctcaatg atgacgtgga gctgaccaca gacttggtga
     4621 gagtgcaccc ggacagcagc ctggtgggtc gtaagggcta cagtaccact gacgggtcgc
     4681 tgtactcgta ctttgaaggt acgaaattca accaggctgc tattgatatg gcagagatac
     4741 tgacgttgtg gcccagactg caagaggcaa acgaacagat atgcctatac gcgctgggcg
     4801 aaacaatgga caacatcaga tccaaatgtc cggtgaacga ttccgattca tcaacacctc
     4861 ccaggacagt gccctgcctg tgccgctacg caatgacagc agaacggatc gcccgcctta
     4921 ggtcacacca agttaaaagc atggtggttt gctcatcttt tcccctcccg aaataccatg
     4981 tagatggggt gcagaaggta aagtgcgaga aggttctcct gttcgacccg acggtacctt
     5041 cagtggttag tccgcggaag tatgccgcat ctacgacgga ccactcagat cggtcgttac
     5101 gagggtttga cttggactgg accaccgact cgtcttccac tgccagcgat accatgtcgc
     5161 tacccagttt gcagtcgtgt gacatcgact cgatctacga gccaatggct cccatagtag
     5221 tgacggctga cgtacaccct gaacccgcag gcatcgcgga cctggcggca gatgtgcacc
     5281 ctgaacccgc agaccatgtg gacctcgaga acccgattcc tccaccgcgc ccgaagagag
     5341 ctgcatacct tgcctcccgc gcggcggagc gaccggtgcc ggcgccgaga aagccgacgc
     5401 ctgccccaag gactgcgttt aggaacaagc tgcctttgac gttcggcgac tttgacgagc
     5461 acgaggtcga tgcgttggcc tccgggatta ctttcggaga cttcgacgac gtcctgcgac
     5521 taggccgcgc gggtgcatat attttctcct cggacactgg cagcggacat ttacaacaaa
     5581 aatccgttag gcagcacaat ctccagtgcg cacaactgga tgcggtccag gaggagaaaa
     5641 tgtacccgcc aaaattggat actgagaggg agaagctgtt gctgctgaaa atgcagatgc
     5701 acccatcgga ggctaataag agtcgatacc agtctcgcaa agtggagaac atgaaagcca
     5761 cggtggtgga caggctcaca tcgggggcca gattgtacac gggagcggac gtaggccgca
     5821 taccaacata cgcggttcgg tacccccgcc ccgtgtactc ccctaccgtg atcgaaagat
     5881 tctcaagccc cgatgtagca atcgcagcgt gcaacgaata cctatccaga aattacccaa
     5941 cagtggcgtc gtaccagata acagatgaat acgacgcata cttggacatg gttgacgggt
     6001 cggatagttg cttggacaga gcgacattct gcccggcgaa gctccggtgc tacccgaaac
     6061 atcatgcgta ccaccagccg actgtacgca gtgccgtccc gtcacccttt cagaacacac
     6121 tacagaacgt gctagcggcc gccaccaaga gaaactgcaa cgtcacgcaa atgcgagaac
     6181 tacccaccat ggactcggca gtgttcaacg tggagtgctt caagcgctat gcctgctccg
     6241 gagaatattg ggaagaatat gctaaacaac ctatccggat aaccactgag aacatcacta
     6301 cctatgtgac caaattgaaa ggcccgaaag ctgctgcctt gttcgctaag acccacaact
     6361 tggttccgct gcaggaggtt cccatggaca gattcacggt cgacatgaaa cgagatgtca
     6421 aagtcactcc agggacgaaa cacacagagg aaagacccaa agtccaggta attcaagcag
     6481 cggagccatt ggcgaccgct tacctgtgcg gcatccacag ggaattagta aggagactaa
     6541 atgctgtgtt acgccctaac gtgcacacat tgtttgatat gtcggccgaa gactttgacg
     6601 cgatcatcgc ctctcacttc cacccaggag acccggttct agagacggac attgcatcat
     6661 tcgacaaaag ccaggacgac tccttggctc ttacaggttt aatgatcctc gaagatctag
     6721 gggtggatca gtacctgctg gacttgatcg aggcagcctt tggggaaata tccagctgtc
     6781 acctaccaac tggcacgcgc ttcaagttcg gagctatgat gaaatcgggc atgtttctga
     6841 ctttgtttat taacactgtt ttgaacatca ccatagcaag cagggtactg gagcagagac
     6901 tcactgactc cgcctgtgcg gccttcatcg gcgacgacaa catcgttcac ggagtgatct
     6961 ccgacaagct gatggcggag aggtgcgcgt cgtgggtcaa catggaggtg aagatcattg
     7021 acgctgtcat gggcgaaaaa cccccatatt tttgtggggg attcatagtt tttgacagcg
     7081 tcacacagac cgcctgccgt gtttcagacc cacttaagcg cctgttcaag ttgggtaagc
     7141 cgctaacagc tgaagacaag caggacgaag acaggcgacg agcactgagt gacgaggtta
     7201 gcaagtggtt ccggacaggc ttgggggccg aactggaggt ggcactaaca tctaggtatg
     7261 aggtagaggg ctgcaaaagt atcctcatag ccatggccac cttggcgagg gacattaagg
     7321 cgtttaagaa attgagagga cctgttatac acctctacgg cggtcctaga ttggtgcgtt
     7381 aatacacaga attctgatta tagcgcacta ttatagcacc atggatcccg tcgttttaca
     7441 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     7501 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     7561 cagcctgaat ggcgaatggc gctttgcctg gtttccggca ccagaagcgg tgccggaaag
     7621 ctggctggag tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa actggcagat
     7681 gcacggttac gatgcgccca tctacaccaa cgtaacctat cccattacgg tcaatccgcc
     7741 gtttgttccc acggagaatc cgacgggttg ttactcgctc acatttaatg ttgatgaaag
     7801 ctggctacag gaaggccaga cgcgaattat ttttgatggc gttaactcgg cgtttcatct
     7861 gtggtgcaac gggcgctggg tcggttacgg ccaggacagt cgtttgccgt ctgaatttga
     7921 cctgagcgca tttttacgcg ccggagaaaa ccgcctcgcg gtgatggtgc tgcgttggag
     7981 tgacggcagt tatctggaag atcaggatat gtggcggatg agcggcattt tccgtgacgt
     8041 ctcgttgctg cataaaccga ctacacaaat cagcgatttc catgttgcca ctcgctttaa
     8101 tgatgatttc agccgcgctg tactggaggc tgaagttcag atgtgcggcg agttgcgtga
     8161 ctacctacgg gtaacagttt ctttatggca gggtgaaacg caggtcgcca gcggcaccgc
     8221 gcctttcggc ggtgaaatta tcgatgagcg tggtggttat gccgatcgcg tcacactacg
     8281 tctgaacgtc gaaaacccga aactgtggag cgccgaaatc ccgaatctct atcgtgcggt
     8341 ggttgaactg cacaccgccg acggcacgct gattgaagca gaagcctgcg atgtcggttt
     8401 ccgcgaggtg cggattgaaa atggtctgct gctgctgaac ggcaagccgt tgctgattcg
     8461 aggcgttaac cgtcacgagc atcatcctct gcatggtcag gtcatggatg agcagacgat
     8521 ggtgcaggat atcctgctga tgaagcagaa caactttaac gccgtgcgct gttcgcatta
     8581 tccgaaccat ccgctgtggt acacgctgtg cgaccgctac ggcctgtatg tggtggatga
     8641 agccaatatt gaaacccacg gcatggtgcc aatgaatcgt ctgaccgatg atccgcgctg
     8701 gctaccggcg atgagcgaac gcgtaacgcg aatggtgcag cgcgatcgta atcacccgag
     8761 tgtgatcatc tggtcgctgg ggaatgaatc aggccacggc gctaatcacg acgcgctgta
     8821 tcgctggatc aaatctgtcg atccttcccg cccggtgcag tatgaaggcg gcggagccga
     8881 caccacggcc accgatatta tttgcccgat gtacgcgcgc gtggatgaag accagccctt
     8941 cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg ctacctggag agacgcgccc
     9001 gctgatcctt tgcgaatacg cccacgcgat gggtaacagt cttggcggtt tcgctaaata
     9061 ctggcaggcg tttcgtcagt atccccgttt acagggcggc ttcgtctggg actgggtgga
     9121 tcagtcgctg attaaatatg atgaaaacgg caacccgtgg tcggcttacg gcggtgattt
     9181 tggcgatacg ccgaacgatc gccagttctg tatgaacggt ctggtctttg ccgaccgcac
     9241 gccgcatcca gcgctgacgg aagcaaaaca ccagcagcag tttttccagt tccgtttatc
     9301 cgggcaaacc atcgaagtga ccagcgaata cctgttccgt catagcgata acgagctcct
     9361 gcactggatg gtggcgctgg atggtaagcc gctggcaagc ggtgaagtgc ctctggatgt
     9421 cgctccacaa ggtaaacagt tgattgaact gcctgaacta ccgcagccgg agagcgccgg
     9481 gcaactctgg ctcacagtac gcgtagtgca accgaacgcg accgcatggt cagaagccgg
     9541 gcacatcagc gcctggcagc agtggcgtct ggcggaaaac ctcagtgtga cgctccccgc
     9601 cgcgtcccac gccatcccgc atctgaccac cagcgaaatg gatttttgca tcgagctggg
     9661 taataagcgt tggcaattta accgccagtc aggctttctt tcacagatgt ggattggcga
     9721 taaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc cgtgcaccgc tggataacga
     9781 cattggcgta agtgaagcga cccgcattga ccctaacgcc tgggtcgaac gctggaaggc
     9841 ggcgggccat taccaggccg aagcagcgtt gttgcagtgc acggcagata cacttgctga
     9901 tgcggtgctg attacgaccg ctcacgcgtg gcagcatcag gggaaaacct tatttatcag
     9961 ccggaaaacc taccggattg atggtagtgg tcaaatggcg attaccgttg atgttgaagt
    10021 ggcgagcgat acaccgcatc cggcgcggat tggcctgaac tgccagctgg cgcaggtagc
    10081 agagcgggta aactggctcg gattagggcc gcaagaaaac tatcccgacc gccttactgc
    10141 cgcctgtttt gaccgctggg atctgccatt gtcagacatg tataccccgt acgtcttccc
    10201 gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat tatggcccac accagtggcg
    10261 cggcgacttc cagttcaaca tcagccgcta cagtcaacag caactgatgg aaaccagcca
    10321 tcgccatctg ctgcacgcgg aagaaggcac atggctgaat atcgacggtt tccatatggg
    10381 gattggtggc gacgactcct ggagcccgtc agtatcggcg gaattccagc tgagcgccgg
    10441 tcgctaccat taccagttgg tctggtgtca aaaataataa taaccgggca ggggggatcc
    10501 cgggtaatta attgacgttt tacggccgcc ggtggcgccc gcgcccggcg gcccgtcctt
    10561 ggccgttgca ggccactccg gtggctcccg tcgtccccga cttccaggcc cagcagatgc
    10621 agcaactcat cagcgccgta aatgcgctga caatgagaca gaacgcaatt gctcctgcta
    10681 ggcctcccaa accaaagaag aagaagacaa ccaaaccaaa gccgaaaacg cagcccaaga
    10741 agatcaacgg aaaaacgcag cagcaaaaga agaaagacaa gcaagccgac aagaagaaga
    10801 agaaacccgg aaaaagagaa agaatgtgca tgaagattga aaatgactgt atcttcgtat
    10861 gcggctagcc acagtaacgt agtgtttcca gacatgtcgg gcaccgcact atcatgggtg
    10921 cagaaaatct cgggtggtct gggggccttc gcaatcggcg ctatcctggt gctggttgtg
    10981 gtcacttgca ttgggctccg cagataagtt agggtaggca atggcattga tatagcaaga
    11041 aaattgaaaa cagaaaaagt tagggtaagc aatggcatat aaccataact gtataacttg
    11101 taacaaagcg caacaagacc tgcgcaattg gccccgtggt ccgcctcacg gaaactcggg
    11161 gcaactcata ttgacacatt aattggcaat aattggaagc ttacataagc ttaattcgac
    11221 gaataattgg atttttattt tattttgcaa ttggttttta atatttccaa aaaaaaaaaa
    11281 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaact
    11341 agtctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
    11401 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
    11461 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
    11521 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
    11581 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
    11641 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
    11701 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    11761 gtggcgcttt ctcaatgctc gcgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    11821 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    11881 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    11941 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    12001 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
    12061 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    12121 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    12181 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    12241 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    12301 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    12361 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    12421 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    12481 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    12541 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    12601 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    12661 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    12721 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    12781 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    12841 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    12901 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    12961 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    13021 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    13081 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    13141 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    13201 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    13261 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    13321 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
    13381 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
    13441 cagctcccgg agacggtcac agcttctgtc taagcggatg ccgggagcag acaagcccgt
    13501 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
    13561 cagattgtac tgagagtgca ccatatcgac gctctccctt atgcgactcc tgcattagga
    13621 agcagcccag tactaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca
    13681 aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac
    13741 aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat
    13801 aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga
    13861 ggatctggct agcgatgacc ctgctgattg gttcgctgac catttccggg gtgcggaacg
    13921 gcgttaccag aaactcagaa ggttcgtcca accaaaccga ctctgacggc agtttacgag
    13981 agagatgata gggtctgctt cagtaagcca gatgctacac aattaggctt gtacatattg
    14041 tcgttagaac gcggctacaa ttaatacata accttatgta tcatacacat acgatttagg
    14101 tgacactata
//
