LOCUS       V003282                 4782 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003282
VERSION     V003282
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Rodriguez MD, Paul Z, Wood CE, Rice KC, Triplett EW.
  TITLE     Construction of Stable Fluorescent Reporter Plasmids for Use in
            Staphylococcus aureus
  JOURNAL   Front Microbiol 8, 2491 (2017)
   PUBMED   29312199
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Rodriguez MD.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2017) Microbiology and Cell Science, University of
            Florida, 1355 Museum Rd., PO Box 110700, Gainesville, FL 32611-0700,
            USA
REFERENCE   3  (bases 1 to 4782)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4782)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front
            Microbiol 8, 2491 (2017)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-AUG-2017) Microbiology and Cell Science, University of Florida,
            1355 Museum Rd., PO Box 110700, Gainesville, FL 32611-0700, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..996
                     /codon_start=1
                     /gene="repF"
                     /product="RepF"
                     /label="repF"
                     /protein_id="AUS76869.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
     gene            1..996
                     /gene="repF"
                     /label="repF"
     rep_origin      1179..1567
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     regulatory      1580..1824
                     /label="sarA P1 promoter"
                     /note="sarA P1 promoter"
                     /regulatory_class="promoter"
     CDS             1847..2329
                     /gene="dfrA"
                     /label="Dihydrofolate reductase type 1"
                     /note="Dihydrofolate reductase type 1 from Tn4003 from
                     Staphylococcus aureus. Accession#: P13955"
     CDS             2367..3068
                     /label="NirFP"
                     /note="near-infrared fluorescent protein eqFP670"
     regulatory      3508..3565
                     /label="atl transcription terminator"
                     /note="atl transcription terminator"
                     /regulatory_class="terminator"
     rep_origin      3962..4139
                     /label="sso"
                     /note="sso"
     CDS             complement(4360..4371)
                     /label="WELQut site"
                     /note="WELQut protease recognition and cleavage site"
     misc_feature    4608..4628
                     /label="dso"
                     /note="dso"
ORIGIN
        1 atgcaatata atactactag aagtataacc gaaaatcaag ataataaaac gttaaaagat
       61 atgacgaaaa gtgggaaaca acgcccatgg agagaaaaga aaatagataa tgtaagctat
      121 gcagatatac tagaaatttt aaaaatcaaa aaggctttta atgtaaaaca atgtggtaat
      181 attttagaat ttaagccaac tgatgaaggc tatttgaagt tacataagac atggttttgt
      241 aaatcaaaat tatgtccggt ttgtaattgg agacgtgcta tgaaaaatag ttatcaagct
      301 caaaaagtga ttgaaaaagt aattaaggaa aagccaaaag cacgttggtt gtttttaaca
      361 ctttcaacaa aaaatgcgat agatggagat actttagaac aaagtttgaa gcatctaact
      421 aaagcatttg ataggttgag tagatataaa aaggttaaac aaaatcttgt tggatttatg
      481 cgttcaacag aagttaccgt taataaaaat gacggtagtt ataatcagca catgcatgtt
      541 ttgttatgtg ttgaaaatgc atattttaga aaaaaagaga attatataac tcaagaagaa
      601 tgggttaatt tatggcaaag agcattacaa gttgactatc gacctgttgc taatgttaaa
      661 gcgatcaaac cgaatagaaa aggcgataaa gacattgaat cggcaatcaa agagacctca
      721 aaatattcgg ttaaatcatc tgatttttta actgatgatg atgaaaaaaa tcaagaaatt
      781 gtaagtgatt tagaaaaagg tttgtatcga aaacgtatgt taagttatgg tggattgctt
      841 aaacaaaaac ataaaatttt aaacttagac gatgtcgaag atggtaattt gattaatgca
      901 agtgatgaag ataaaacaac agacgaagaa gaaaaagcac attcaattac cgcaatttgg
      961 aatttcgaaa agcaaaatta ttatttaaga cattagtgtt gactaatgtc ttttttgttg
     1021 attttttata aaaaagtact gtcttatttt tgtgacaaat gctgtatgta gtgtcacaaa
     1081 aataagacaa acgcaatata ttgtgtcaca aaaataagac agtacagctt tgtatgatcc
     1141 gtcgacgaaa gcctggccac gatgcgtccg gcgtagagga tctgaagatc agcagttcaa
     1201 cctgttgata gtacgtacta agctctcatg tttcacgtac taagctctca tgtttaacgt
     1261 actaagctct catgtttaac gaactaaacc ctcatggcta acgtactaag ctctcatggc
     1321 taacgtacta agctctcatg tttcacgtac taagctctca tgtttgaaca ataaaattaa
     1381 tataaatcag caacttaaat agcctctaag gttttaagtt ttataagaaa aaaaagaata
     1441 tataaggctt ttaaagcttt taaggtttaa cggttgtgga caacaagcca gggatgtaac
     1501 gcactgagaa gcccttagag cctctcaaag caattttgag tgacacagga acacttaacg
     1561 gctgacatgg gaattcgagc tgatattttt gactaaacca aatgctaacc cagaaataca
     1621 atcactgtgt ctaatgaata atttgtttta taaacacttt tttgtttact tctcattttt
     1681 aattagttat aattaactaa ataatagagc attaaatata tttaataaaa cttatttaat
     1741 gcaaaattat gactaacata tctataataa ataaagatta gatatcaata tattatcggg
     1801 caaatgtatc gagcaagatg catcggatcg atccaggagg tataccatga cattatcaat
     1861 aattgtcgct cacgataaac aaagagtcat tgggtaccaa aatcaattac cttggcactt
     1921 accaaatgat ttaaagcata ttaaacaact gaccactggg aatacacttg taatggcacg
     1981 gaaaactttt aattctatag ggaagccatt gccaaataga cgtaacgtcg tactcactaa
     2041 ccaagcttca tttcgccatg aaggggtaga tgttataaac tctcttgatg aaattaaaga
     2101 gttatctggt catgttttta tatttggagg acaaacgtta tacgaagcaa tgattgacca
     2161 ggtagatgat atgtatatca cagtaataga tggaaagttt caaggagaca cattctttcc
     2221 accatacaca ttcgaaaact gggaagtcga atcttcagta gaaggtcaac tagatgaaaa
     2281 aaatactata ccgcatacat tcttacattt agtgcgtaga aaagggaaat aggcgcgcct
     2341 attaacttta taaggaggaa aaacatatgg gtgaagatag tgaattaatt agtgaaaata
     2401 tgcacatgaa attatatatg gaaggtactg taaatggtca tcattttaaa tgtacttcag
     2461 aaggtgaagg taaaccttat gaaggtacac aaactgcaaa aattaaagtt gtagaaggtg
     2521 gtccattacc ttttgcattt gatattttag ctacatcatt tatgtatggt agtaaaacat
     2581 ttattaatca tactcaaggt attccagatt tctttaaaca atcttttcct gaaggtttta
     2641 catgggaacg tattacaact tatgaagatg gtggtgtttt aacagcaact caagatacaa
     2701 gtttacaaaa tggttgttta atttataatg ttaaaattaa tggtgtaaat tttccatcaa
     2761 atggtcctgt aatgcaaaag aaaacattag gttgggaagc aagtactgaa atgttatatc
     2821 cagctgattc tggtttacgt ggtcattcac aaatggcttt aaaattagtt ggtggtggtt
     2881 atttacattg tagtttaaaa acaacttata gatctaaaaa accagcaaaa aatttaaaaa
     2941 tgcctggttt ttattttgtt gatcgtaaat tagaaagaat taaagaagct gataaagaaa
     3001 cttatgttga acaacatgaa atggctgtag ctcgttattg tgatttacca tcaaaattag
     3061 gtcatagtta ataggcgcgc ctattctaat gcataataaa tactgataac atcttatatt
     3121 ttgtattata ttttgtatta tcgttgacat gtataatttt gatatcaaaa actgattttc
     3181 cctctattat tttcgagatt tattttctta attctcttta acaaactaga aatattgtat
     3241 atacaaaaaa ttataaataa tagatgaata gtttaattat aggtgttcat caatcgaaaa
     3301 agcaacgtat cttatttaaa gtgcgttgct tttttctcat ttataaggtt aaataattct
     3361 catatatcaa gcaaagtgac aggcgatgcg gccgctagcc taggagctcg gtacccgggg
     3421 atccgaatca tgaattacaa gcaaaagtag cggtgattgt taaaattgat ggtaaacaat
     3481 caccgctatt tttgcttgtg tatgtataaa aaagggatca aaggtcatcc cccatgattg
     3541 atagtggggg gatgactttt gatcctatgt tcatgttgct tatttaatcg cctttgatca
     3601 ctttaaaata ccttaaaacc cctggaattt ctggctttgc cagacctatc atttttgaat
     3661 gatagcaaat tctccttatg ctcttacgga gtttttagag aaaaattaaa aattctcgat
     3721 ttttgataaa aaacgccctg caggaattta gaaaaacatg tggaagtttt aaaggatttt
     3781 atgctaattt ttaatttgca tgtaactcga ggggaatatt tgaggggatt ttgaaacgag
     3841 tttcttcttg ttttcacact gtttttttat tcctattggt gttgttgctt actttttgtt
     3901 tttctataaa gatgatgctt ttgacgagat ggaagaaaaa tatgattatc atgaagataa
     3961 taaaaaatag acgacgcatt tatgccgaga aaatttattg atgttgagaa gaacccttaa
     4021 ctaaacttgc agacgaatgt cggcatagcg tgagctatta agccgaccat tcgacaagtt
     4081 ttgggattgt taagggttcc gaggctcaac gtcaataaag caattggaat aaatagtatc
     4141 taaagtaacc cgtatttaag attacattgt acatccttcg aaaccatagt agctgcgatt
     4201 ccagctaggt ttccttttat cccaatttta gctaattttt ttgctacgcc ctttaagttt
     4261 ttggctttaa catcttcaaa aactgctgtg attatgtttc cagttaaaag ttctccatat
     4321 gagtttaaaa cttcagaata gaaacagtca ccgctatttt tgtagttccc accaactgct
     4381 ctaggtgata ttgaactagt tttcttttta ttctctatgt cgttttttag tttttggaat
     4441 ccttcaggaa catatccata tcgagatttg gccgatttta tttgataatc cttttgactt
     4501 tttagaaagt tttatttatt cattggtttt cacattaatt tatttaggtt tagagggtca
     4561 taaaaagaag aaggaatagg ttgttttttg aaacgagtgt gaacgagttt cttcttgtct
     4621 tgatactata tagaaataac tcgattttat atatatagct gtaactgttg atattacagt
     4681 gtttaaacgt gtttttgtgc gtgaaaggaa aatttgacaa taaaaaaccc cagttatatt
     4741 attaaggtgt cgaatcttaa ataatactgg gggtcttttt at
//
