LOCUS       62056_19600       11192 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11192)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..28)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(36..52)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(60..90)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(105..126)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(414..1002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1176..2033)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2034..2138)
                     /label=AmpR promoter
     protein_bind    complement(3710..3757)
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        3798..4174
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     intron          4184..4316
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        4361..4379
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4458..4514
                     /codon_start=1
                     /label=GCN4_v4
                     /note="GCN4 peptide optimized to improve solubility while 
                     preserving binding to an scFv-GFP fusion protein (Tanenbaum
                     et al., 2014)"
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6204..7133
                     /codon_start=1
                     /label=hRluc
                     /note="Renilla luciferase"
                     /translation="ASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENA
                     VIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAWF
                     ELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDIA
                     LIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREI
                     PLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVK
                     GLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             7134..7457
                     /codon_start=1
                     /label=DD
                     /note="destabilization domain that can be stabilized by
                     Shield1 in the ProteoTuner(TM) system"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     polyA_signal    complement(8990..9124)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(9302..9709)
                     /codon_start=1
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /translation="VTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTFNVG
                     WPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKPPPP
                     LPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     misc_feature    complement(9783..10140)
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     LTR             complement(10203..10795)
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
ORIGIN
        1 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
       61 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      121 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      181 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      241 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      301 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      361 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      421 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      481 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      541 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      601 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      661 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      721 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      781 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      841 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      901 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      961 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1021 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt tgtcatgaga
     1081 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1141 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1201 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1261 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1321 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1381 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1441 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1501 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1561 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1621 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1681 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1741 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1801 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1861 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1921 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1981 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2041 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2101 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2161 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2221 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     2281 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     2341 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     2401 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     2461 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     2521 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     2581 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attaattcca
     2641 agctaagtga aagactctgt ctcaaaaacc aacctgacac ctgagccgga gcgttgcctc
     2701 tcactaccat aaatatatat acacaacaca cacacacaca cacacacaca cacacacaca
     2761 cacacacaca atcatacaca taaacacaat cacacacaca cacatgcaca atcacacaca
     2821 cacaatcaca cacaaacata catacacaca atcacacaca accatacaca catatacaca
     2881 atcacacaca caaacacaat cacacacaca cacatcaatc aatcaatcaa tcaatatacc
     2941 aaaaatgata gctcatacct gtaatcttag atgtgggaag gctaaaacag caggctaccc
     3001 atgacttcaa ggtcaccctg ggttacagag tagctaatag tctagcttga actatagtgt
     3061 aggatcttta aaaaaaaatt aaccaaccaa ccaacaaaca aacaaacaaa caacaacaac
     3121 aacaaatgga agacagagga caaaaaaaaa taaaagaaaa agtagaaagc caatggagat
     3181 actatcttaa aattagaaat atttatgaca cattctgaat actccatgtt tatttcaagg
     3241 aagccaatgt aactttaagt actatggcca tttaatgtaa atacttaaga aaaaaaacca
     3301 aattaatttt gatacatgct gcatgtgaaa gacccccgct gacgggtagt caatcactca
     3361 gaggagaccc tctcaaggaa cagcgagacc acaagtcgga tgcaactgca agagggttta
     3421 ttggatacac gggtacccgg gcgactcagt caatcggagg actggcgccc cgagtgaggg
     3481 gttgtgggct cgagctcggg gagcagaagc gcgcgaacag aagtcgagct agcttgccaa
     3541 acctacaggt ggggtctttc attcccccct ttttctggag actaaataaa atcttttatt
     3601 ttatctatgg ctcgtactct ataggcttca gctggtgata ttgttgagtc aaaactagag
     3661 cctggaccac tgatatcctg tctttaacaa attggactaa tccggatctg aagttcctat
     3721 actttctaga gaataggaac ttcggaatag gaacttcgcg gccagatctt ttgcggccgc
     3781 tttgggccgg ccgaattctt tactccctat cagtgataga gaacgtatga agagtttact
     3841 ccctatcagt gatagagaac gtatgcagac tttactccct atcagtgata gagaacgtat
     3901 aaggagttta ctccctatca gtgatagaga acgtatgacc agtttactcc ctatcagtga
     3961 tagagaacgt atctacagtt tactccctat cagtgataga gaacgtatat ccagtttact
     4021 ccctatcagt gatagagaac gtataagctt taggcgtgta cggtgggcgc ctataaaagc
     4081 agagctcgtt tagtgaaccg tcagatcgcc tggagcaatt ccacaacact tttgtcttat
     4141 accaactttc cgtaccactt cctaccctcg taaaaccggt caggtaagta tcaaggttac
     4201 aagacaggtt taaggagacc aatagaaact gggcttgtcg agacagagaa gactcttgcg
     4261 tttctgatag gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgt
     4321 ccactcccag ttcaattaca gctcttaagg ctagagtact taatacgact cactataggc
     4381 tagccaccat gggcgccatc atacacgcgg ccgcaatcat cggtccgggt ggatctggag
     4441 gtggaggttc tggaggagaa gaacttttga gcaagaatta tcatcttgag aacgaagtgg
     4501 ctcgtcttaa gaaaggttct ggcagtggag aagaactgct ttcaaagaat taccacctgg
     4561 aaaatgaggt agctagactg aaaaagggga gcggaagtgg ggaggagttg ctgagcaaaa
     4621 attatcattt ggagaacgaa gtagcacgac taaagaaagg gtccggatcg ggtgaggagt
     4681 tactctcgaa aaattatcat ctcgaaaacg aagtggctcg gctaaaaaag ggcagtggtt
     4741 ctggagaaga gctattatct aaaaactacc acctcgaaaa tgaggtggca cgcttaaaaa
     4801 agggaagtgg cagtggtgaa gagctactat ccaagaatta tcatcttgag aacgaggtag
     4861 cgcgtttgaa gaagggttcc ggctcaggag aggaactgct ctcgaagaac tatcatcttg
     4921 aaaatgaggt cgctcgatta aaaaagggat cgggcagtgg tgaggaacta ctttcaaaga
     4981 attaccacct cgaaaacgaa gtagctcgat taaagaaagg ttcagggtcg ggtgaagaat
     5041 tactgagtaa aaattatcat ctggaaaatg aggtagcgag actaaaaaag gggagtggtt
     5101 ctggcgaaga gttgctatcg aaaaattatc atcttgagaa cgaagttgct aggctcaaaa
     5161 agggctcagg ctcaggcgag gagttgctct cgaaaaacta ccacttggaa aatgaggtcg
     5221 cgaggttgaa aaaggggagc gggtcgggcg aggagttatt gagcaaaaac tatcatttag
     5281 agaacgaagt cgcgcgctta aagaaaggct cgggctcggg cgaagaactc ttatcgaaga
     5341 actaccacct cgaaaatgag gtcgccaggt tgaaaaaggg cagtggcagc ggggaggaac
     5401 tcttgagcaa gaactaccac ttggagaatg aggtcgcgag attgaagaaa gggtcgggga
     5461 gcggcgagga attgctcagc aagaattatc atttggagaa cgaagtcgcc aggctcaaga
     5521 aaggctcggg gtcgggggag gaattgttga gtaaaaacta ccacttggaa aatgaagtcg
     5581 ccaggctcaa aaaagggagt gggagcggcg aagagttatt gagcaaaaat taccacttgg
     5641 agaacgaagt ggcaaggctc aagaaaggga gcggcagcgg ggaggagctc ttatcgaaga
     5701 actaccactt agagaatgaa gtcgcccgct tgaagaaagg ctcggggagc ggggaagagc
     5761 tcttgagcaa gaactaccac ttggaaaatg aggtggcgcg cttgaagaaa gggagcggga
     5821 gcggggaaga gttactatct aagaattatc atctcgagaa cgaggtggct cgactaaaga
     5881 agggctccgg cagtggggag gaactcctgt cgaagaacta tcatcttgaa aatgaggttg
     5941 caagacttaa aaaggggtcc ggatcaggtg aggaactact cagtaagaat taccacctgg
     6001 aaaacgaagt agctcgatta aagaaaggtt cagggtcggg tgaagaactg ctctcaaaag
     6061 attatcattt ggaaaatgag gttgcacgtt taaaaaaggg aagtggcagt ggtgaggaac
     6121 ttctgtcgaa aaattatcat ctcgagaatg aagtagcccg acttaaaaag ggttctggct
     6181 cgggtcagcg gccgcaccgg tcagcttcca aggtgtacga ccccgagcaa cgcaaacgca
     6241 tgatcactgg gcctcagtgg tgggctcgct gcaagcaaat gaacgtgctg gactccttca
     6301 tcaactacta tgattccgag aagcacgccg agaacgccgt gatttttctg catggtaacg
     6361 ctgcctccag ctacctgtgg aggcacgtcg tgcctcacat cgagcccgtg gctagatgca
     6421 tcatccctga tctgatcgga atgggtaagt ccggcaagag cgggaatggc tcatatcgcc
     6481 tcctggatca ctacaagtac ctcaccgctt ggttcgagct gctgaacctt ccaaagaaaa
     6541 tcatctttgt gggccacgac tggggggctt gtctggcctt tcactactcc tacgagcacc
     6601 aagacaagat caaggccatc gtccatgctg agagtgtcgt ggacgtgatc gagtcctggg
     6661 acgagtggcc tgacatcgag gaggatatcg ccctgatcaa gagcgaagag ggcgagaaaa
     6721 tggtgcttga gaataacttc ttcgtcgaga ccatgctccc aagcaagatc atgcggaaac
     6781 tggagcctga ggagttcgct gcctacctgg agccattcaa ggagaagggc gaggttagac
     6841 ggcctaccct ctcctggcct cgcgagatcc ctctcgttaa gggaggcaag cccgacgtcg
     6901 tccagattgt ccgcaactac aacgcctacc ttcgggccag cgacgatctg cctaagatgt
     6961 tcatcgagtc cgaccctggg ttcttttcca acgctattgt cgagggagct aagaagttcc
     7021 ctaacaccga gttcgtgaag gtgaagggcc tccacttcag ccaggaggac gctccagatg
     7081 aaatgggtaa gtacatcaag agcttcgtgg agcgcgtgct gaagaacgag cagatgggag
     7141 tgcaggtgga aaccatctcc ccaggagacg ggcgcacctt ccccaagcgc ggccagacct
     7201 gtgtggtgca ctacaccggg atgcttgaag atggaaagaa agtcgattcc tcccgggaca
     7261 gaaacaagcc ctttaagttt atgctaggca agcaggaggt gatccgaggc tgggaagaag
     7321 gggttgccca gatgagtgtg ggtcagagag ccaaactgac tatatctcca gattatgcct
     7381 atggtgccac tgggcaccca ggcatcatcc caccacatgc cactctcgtc ttcgatgtgg
     7441 agcttctaaa accggaataa gggccaggga agaactggct cggaaagagg accccacatg
     7501 ttgtagcttc aagtttaaac ggatccaacc tacaaacggg tggaggatca ccccacccga
     7561 cacttcacaa tcaaggggta caatacacaa gggtggagga acaccccacc ctccagacac
     7621 attacacaga aatccaatca aacagaagca ccatcagggc ttctgctacc aaatttatct
     7681 caaaaaacta caacaaggaa tcaccatcag ggattccctg tgcaatatac gtcaaacgag
     7741 ggccacgacg ggaggacgat cacgcctccc gaatatcggc atgtctggct ttcgaattca
     7801 gtgcgtggag catcagccca cgcagccaat cagagtcgaa tacaagtcga ctttcgcgaa
     7861 gagcatcagc cttcgcgcca ttcttacaca aaccacactc tcccctacag gaacagcatc
     7921 agcgttcctg cccagtaccc aactcaagaa aatttatgtc cccatgcagc atcagcgcat
     7981 gggccccaag aatacatccc caacaaaatc acatccgagc accaacaggg ctcggagtgt
     8041 tgtttcttgt ccaactggac aaaccctcca tggaccatca ggccatggac tctcaccaac
     8101 aagacaaaaa ctactcttct cgaagcagca tcagcgcttc gaaacactcg agcatacatt
     8161 gtgcctattt cttgggtgga cgatcacgcc acccatgctc tcacgaattt caaaacacgg
     8221 acaaggacga gcaccaccag ggctcgtcgt tccacgtcca atacgattac ttacctttcg
     8281 ggatcacgat cacggatccc gcagctacat cacttccact caggacattc aagcatgcac
     8341 gatcacggca tgctccacaa gtctcaacca cagaaactac caaatgggtt cagcaccagc
     8401 gaacccactc ctacctcaaa cctcttccca caaaactggc aagcaggatc accgcttgcc
     8461 cattccaaca taccaaatca aaaacaatta ctggtacagc atcagcgtac cagcccacat
     8521 ctctcactac tatcaaaaac caaaccgttc agcaacagcg aacggtacac acggaaaaat
     8581 caactggttt acaaatacga aagacgatca cgctttcgtc cagcgcaaac tattacgaaa
     8641 aacatccgac gggaagagca acagccttcc cgcggcggaa aacctcacaa aaacacgaca
     8701 aacggatgca cgaacacggc atccgccgac aacccacaaa cttacaacca ggcaaacggt
     8761 gcaggatcac cgcaccgtac atcaaacacc tcagatctca tttcgcgcgg gatccagacc
     8821 acctcccctg cgagctaagc tggacagcca atgacgggta agagagtgac atttttcact
     8881 aacctaagac aggagggccg tcagagctac tgcctaatcc aaagacgggt aaaagtgata
     8941 aaaatgtatc actccaacct aagacaggcg cagcttccga gggatttgag atccagacat
     9001 gataagatac attgatgagt ttggacaaac caaaactaga atgcagtgaa aaaaatgcct
     9061 tatttgtgaa atttgtgatg ctattgcctt atttgtaacc attataagct gcaataaaca
     9121 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     9181 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc tgaagttcct
     9241 atactatttg aagaatagga acttcggaat aggaacttcg gatctcatat ggccatatgg
     9301 gggcgcctag agaaggagtg agggctggat aaagggagga tcgaggcggg gtcgaacgag
     9361 gaggttcaag ggggagagac ggggcggatg gaggaagagg aggcggaggc ttagggtgta
     9421 caaagggctt gacccaggga ggggggtcaa aagccaaggc ttcccaggtc acgatgtagg
     9481 ggacctggtc tgggtgtcca tgcgggccag gtgaaaagac cttgatctta acctgggtga
     9541 tgaggtctcg gttaaaggtg ccgtctcgcg gccatccgac gttaaaggtt ggccattctg
     9601 cagagcagaa ggtaacccaa cgtctcttct tgacatctac cgactggttg tgagcgatcc
     9661 gctcgacatc tttccagtga cctaaggtca aacttaaggg agtggtaaca ggctagcccg
     9721 ggcccatatt ttcagacaaa tacagaaaca cagtcagaca gagacaacac agaacgatgc
     9781 tgcagcagac aagacgcgcg gcgcggcttc ggtcccaaac cgaaagcaaa aattcagacg
     9841 gaggcgggaa ctgttttagg ttctcgtctc ctaccagaac cacatatccc tcctctaagg
     9901 ggggtgcacc aaagagtcct aaacgatcgg gattttagga ctcaggtcgg gccacaaaaa
     9961 cggcccccga agtccctggg acgtctccca gggttgcggc cgggtgttcc gaactcgtca
    10021 gttccaccac gggtccgcca gatacagagc tagttagcta actagtaccg acgcaggcgc
    10081 ataaaatcag tcatagacac tagacaatcg gacagacaca gataagttgc tggccagctt
    10141 acctcccggt ggtgggtcgg tggtccctgg gcaggggtct cccgatcccg gacgagcccc
    10201 caaatgaaag acccccgctg acgggtagtc aatcactcag aggagaccct cccaaggaac
    10261 agcgagacca caagtcggat gcaactgcaa gagggtttat tggatacacg ggtacccggg
    10321 cgactcagtc aatcggagga ctggcgcgcc gagtgagggg ttgtgggctc ttttattgag
    10381 ctcggggagc agaagcgcgc gaacagaagc gagaagcgaa ctgattggtt agttcaaata
    10441 aggcacaggg tcatttcagg tccttggggc accctggaaa catctgatgg ttctctagaa
    10501 actgctgagg gctggaccgc atctggggac catctgttct tggccctgag ccggggcagg
    10561 aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct gttcttggcc
    10621 ctgagccggg gcaggaactg cttaccacag atatcctgtt tggcccatat tcagctgttc
    10681 catctgttcc tgaccttgat ctgaactttt ctattctcag ttatgtattt ttccatgcct
    10741 tgcaaaatgg cgttacttaa gctagcttgc caaacctaca ggtggggtct ttcacatgta
    10801 tatgtcaaaa ataaaaatca actaattgac tagtaattaa tatgactggc ataatgggaa
    10861 attgatcctg acagatgcaa actggcttct cagcagcgca tttatgttgt caactgagga
    10921 aggaaacgtt aatgacagaa actctaagta atttccacgt ttatctattt ttatttatac
    10981 tagctttggt aacaggaata ttgcagcatt catgcacatt gaaaccctta tgaaataaaa
    11041 acatctgtgc atttaaaatg gaattaacat tttaaatgtt aaaaaaagct ggcttagctt
    11101 ccccccgccc cctagggcat agaacaagtc aaatgcttta tatatttgag tttgggatgt
    11161 attaggaaac tcctaagagc aaagctagct tg
//
