LOCUS       pSF-pA-PromMCS-R        4768 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for low-background measurements of promoter activity using 
            Renilla luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pSF-pA-PromMCS-RLuc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Oxford Genetics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4768)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..89
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     terminator      complement(94..141)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    complement(158..206)
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     polyA_signal    241..289
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    303..394
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     misc_feature    395..432
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     RBS             512..517
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             526..1461
                     /label=RLuc
                     /note="Renilla luciferase"
     misc_feature    1484..1494
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     misc_feature    1532..1542
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    complement(1574..1695)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     terminator      1809..1856
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      1886..2022
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    2067..2138
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     rep_origin      complement(2364..2952)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     RBS             3685..3690
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             3698..4489
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      4505..4641
                     /note="rrnG terminator"
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 gcgatcgcgg ctcccgacat cttggaccat tagctccaca ggtatcttct tccctctagt
       61 ggtcataaca gcagcttcag ctacctctca attcaaaaaa cccctcaaga cccgtttaga
      121 ggccccaagg ggttatgcta tcaatcgttg cgttacacac acaaaaaacc aacacacatc
      181 catcttcgat ggatagcgat tttattatct aactgctgat cgagtgtagc cagatctgat
      241 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgaaagta
      301 ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc
      361 cccagtgcaa atgcaggtgc cagaacattt ctctgtcgac caactagtac cccgggtagg
      421 gcccaattcg aagtagatct ttgtcgatcc taccatccac tcgacacacc cgccagcggc
      481 cgctgccaag cttccgagct ctcgaattca aaggaggtac ccaccatgac cagcaaagtg
      541 tatgatccgg aacagcgcaa acgcatgatt accggcccgc agtggtgggc gcgctgcaaa
      601 cagatgaacg tgctggatag ctttattaac tattatgata gcgaaaaaca tgcggaaaac
      661 gcggtgattt ttctgcatgg caacgcggcg agcagctatc tgtggcggca tgtggtgccg
      721 catattgaac cagtggcgcg ctgcattatt ccggatctga ttggcatggg caaaagcggc
      781 aaaagcggca acggcagcta tcgcctgctg gatcattata aatatctgac cgcgtggttt
      841 gaactgctga acctgccgaa aaaaattatt tttgtgggcc atgattgggg cgcgtgcctg
      901 gcgtttcatt atagctatga acatcaggat aaaattaaag cgattgtgca cgcggaaagc
      961 gtggtggatg tgattgaaag ctgggatgaa tggccggata ttgaagaaga tattgcgctg
     1021 attaaaagcg aagaaggcga aaaaatggtg ctggaaaaca acttttttgt ggaaaccatg
     1081 ctgccgagca aaattatgcg caaactggaa ccggaagaat ttgcggcgta tctggaaccg
     1141 tttaaagaaa aaggcgaagt gcgccgcccg accctgagct ggccgcgcga aattccgctg
     1201 gtgaaaggcg gcaaaccgga tgtggtccag attgtgcgca actataacgc gtatctgcgc
     1261 gcgagcgatg atctgccgaa aatgtttatt gaaagcgatc cgggcttttt tagcaacgcg
     1321 attgtggaag gcgcgaaaaa atttccgaac accgaatttg tgaaagtgaa gggcctgcat
     1381 tttagccagg aagatgcgcc ggatgaaatg ggcaaatata ttaagagctt tgtggaacgc
     1441 gtgctgaaaa acgaacaggg ctagaagttg tctcctcctg cactgactga ctgatacaat
     1501 cgatttctgg atccgcaggc ctctgctagc ttgactgact gagatacagc gtaccttcag
     1561 ctcacagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     1621 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     1681 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     1741 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtattgg cccatctcta
     1801 tcggtatcgt agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgtgcc
     1861 cctcgggccg gattgctatc taccggcatt ggcgcagaaa aaaatgcctg atgcgacgct
     1921 gcgcgtctta tactcccaca tatgccagat tcagcaacgg atacggcttc cccaacttgc
     1981 ccacttccat acgtgtcctc cttaccagaa atttatcctt aaggtcgtca gctatcctgc
     2041 aggcgatctc tcgatttcga tcaagacatt cctttaatgg tcttttctgg acaccactag
     2101 gggtcagaag tagttcatca aactttcttc cctccctaat ctcattggtt accttgggct
     2161 atcgaaactt aattaaccag tcaagtcagc tacttggcga gatcgacttg tctgggtttc
     2221 gactacgctc agaattgcgt cagtcaagtt cgatctggtc cttgctattg cacccgttct
     2281 ccgattacga gtttcattta aatcatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2341 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2401 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2461 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2521 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2581 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2641 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2701 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2761 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2821 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2881 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2941 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3001 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     3061 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     3121 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     3181 atagttgcat ttaaatttcc gaactctcca aggccctcgt cggaaaatct tcaaaccttt
     3241 cgtccgatcc atcttgcagg ctacctctcg aacgaactat cgcaagtctc ttggccggcc
     3301 ttgcgccttg gctattgctt ggcagcgcct atcgccaggt attactccaa tcccgaatat
     3361 ccgagatcgg gatcacccga gagaagttca acctacatcc tcaatcccga tctatccgag
     3421 atccgaggaa tatcgaaatc ggggcgcgcc tggtgtaccg agaacgatcc tctcagtgcg
     3481 agtctcgacg atccatatcg ttgcttggca gtcagccagt cggaatccag cttgggaccc
     3541 aggaagtcca atcgtcagat attgtactca agcctggtca cggcagcgta ccgatctgtt
     3601 taaacctaga tattgatagt ctgatcggtc aacgtataat cgagtcctag cttttgcaaa
     3661 catctatcaa gagacaggat cagcaggagg ctttcgcatg attgaacaag atggattgca
     3721 cgcaggttct ccggcggctt gggtggagag gctattcggc tatgactggg cacaacagac
     3781 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgtc cggttctttt
     3841 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
     3901 gtggctggcg acgacgggcg ttccttgcgc ggctgtgctc gacgttgtca ctgaagcggg
     3961 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     4021 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     4081 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     4141 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     4201 cgaactgttc gccaggctca aggcgtctat gcccgacggc gaggatctcg tcgtgaccca
     4261 cggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     4321 ctgtggccgt ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     4381 tgctgaagag cttggcggcg aatgggctga ccgcttcctt gtgctttacg gtatcgccgc
     4441 gcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaccgattct
     4501 aggtgcattg gcgcagaaaa aaatgcctga tgcgacgctg cgcgtcttat actcccacat
     4561 atgccagatt cagcaacgga tacggcttcc ccaacttgcc cacttccata cgtgtcctcc
     4621 ttaccagaaa tttatcctta aggtcgttta aactcgactc tggctctatc gaatctccgt
     4681 cgtttcgagc ttacgcgaac agccgtggcg ctcatttgct cgtcgggcat cgaatctcgt
     4741 cagctatcgt cagcttacct ttttggca
//
