LOCUS       pSF-pA-PromMCS-F        5482 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for low-background measurements of promoter activity using 
            firefly luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pSF-pA-PromMCS-FLuc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Oxford Genetics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5482)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5482
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..89
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     terminator      complement(94..141)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    complement(158..206)
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     polyA_signal    241..289
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    303..394
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     misc_feature    395..432
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     RBS             512..517
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             526..2175
                     /label=luciferase
                     /note="firefly luciferase"
     misc_feature    2198..2208
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     misc_feature    2246..2256
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    complement(2288..2409)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     terminator      2523..2570
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      2600..2736
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    2781..2852
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     rep_origin      complement(3078..3666)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     RBS             4399..4404
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             4412..5203
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      5219..5355
                     /note="rrnG terminator"
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 gcgatcgcgg ctcccgacat cttggaccat tagctccaca ggtatcttct tccctctagt
       61 ggtcataaca gcagcttcag ctacctctca attcaaaaaa cccctcaaga cccgtttaga
      121 ggccccaagg ggttatgcta tcaatcgttg cgttacacac acaaaaaacc aacacacatc
      181 catcttcgat ggatagcgat tttattatct aactgctgat cgagtgtagc cagatctgat
      241 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgaaagta
      301 ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc
      361 cccagtgcaa atgcaggtgc cagaacattt ctctgtcgac caactagtac cccgggtagg
      421 gcccaattcg aagtagatct ttgtcgatcc taccatccac tcgacacacc cgccagcggc
      481 cgctgccaag cttccgagct ctcgaattca aaggaggtac ccaccatgga agatgccaaa
      541 aacattaaga aaggcccagc gccattctac ccactcgaag acgggaccgc tggcgagcag
      601 ctgcataaag ccatgaagcg ctacgccctg gtgcccggca ccatcgcctt taccgacgca
      661 catatcgagg tggacattac ctacgccgag tacttcgaga tgagcgttcg gctggcagaa
      721 gctatgaagc gctatgggct gaatacaaac catcggatcg tggtgtgtag cgagaatagc
      781 ttgcagttct tcatgcccgt gttgggtgcc ctgttcatcg gtgtggctgt ggccccagct
      841 aacgacatct acaacgagcg cgagctgctg aacagcatgg gcatcagcca gcccaccgtc
      901 gtattcgtga gcaagaaagg gctgcaaaag atcctcaacg tgcaaaagaa gctaccgatc
      961 atacaaaaga tcatcatcat ggatagcaag accgactacc agggcttcca aagcatgtac
     1021 accttcgtga cttcccattt gccacccggc ttcaacgagt acgacttcgt gcccgagagc
     1081 ttcgaccggg acaaaaccat cgccctgatc atgaacagta gtggcagtac cggattgccc
     1141 aagggcgtag ccctaccgca ccgcaccgct tgtgtccgat tcagtcatgc ccgcgacccc
     1201 atcttcggca accagatcat ccccgacacc gctatcctga gcgtggtgcc atttcaccac
     1261 ggcttcggca tgttcaccac gctgggctac ttgatctgcg gctttcgggt cgtgctcatg
     1321 taccgcttcg aggaagagct attcttgcgc agcttgcaag actataagat tcaatctgcc
     1381 ctgctggtgc ccacactatt tagcttcttc gctaagagca ctctcatcga caagtacgac
     1441 ctaagcaact tgcacgagat cgccagcggc ggggcgccgc tcagcaagga ggtaggtgag
     1501 gccgtggcca aacgcttcca cctaccaggc atccgccagg gctacggcct gacagaaaca
     1561 accagcgcca ttctgatcac ccccgaaggg gacgacaagc ctggcgcagt aggcaaggtg
     1621 gtgcccttct tcgaggctaa ggtggtggac ttggacaccg gtaagacact gggtgtgaac
     1681 cagcgcggcg agctgtgcgt ccgtggcccc atgatcatga gcggctacgt taacaacccc
     1741 gaggctacaa acgctctcat cgacaaggac ggctggctgc atagcggcga catcgcctac
     1801 tgggacgagg acgagcactt cttcatcgtg gaccggctga agagcctgat caaatacaag
     1861 ggctaccagg tagccccagc cgaactggag agcatcctgc tgcaacaccc caacatcttc
     1921 gacgccgggg tcgccggcct gcccgacgac gatgccggcg agctgcccgc cgcagtcgtc
     1981 gtgctggaac acggtaaaac catgaccgag aaggagatcg tggactatgt ggccagccag
     2041 gttacaaccg ccaagaagct gcgcggtggt gttgtgttcg tggacgaggt gcctaaagga
     2101 ctgaccggca agttggacgc ccgcaagatc cgcgagattc tcattaaggc caagaagggc
     2161 ggcaagatcg ccgtctagaa gttgtctcct cctgcactga ctgactgata caatcgattt
     2221 ctggatccgc aggcctctgc tagcttgact gactgagata cagcgtacct tcagctcaca
     2281 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     2341 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     2401 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     2461 gaggtttttt aaagcaagta aaacctctac aaatgtggta ttggcccatc tctatcggta
     2521 tcgtagcata accccttggg gcctctaaac gggtcttgag gggttttttg tgcccctcgg
     2581 gccggattgc tatctaccgg cattggcgca gaaaaaaatg cctgatgcga cgctgcgcgt
     2641 cttatactcc cacatatgcc agattcagca acggatacgg cttccccaac ttgcccactt
     2701 ccatacgtgt cctccttacc agaaatttat ccttaaggtc gtcagctatc ctgcaggcga
     2761 tctctcgatt tcgatcaaga cattccttta atggtctttt ctggacacca ctaggggtca
     2821 gaagtagttc atcaaacttt cttccctccc taatctcatt ggttaccttg ggctatcgaa
     2881 acttaattaa ccagtcaagt cagctacttg gcgagatcga cttgtctggg tttcgactac
     2941 gctcagaatt gcgtcagtca agttcgatct ggtccttgct attgcacccg ttctccgatt
     3001 acgagtttca tttaaatcat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     3061 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     3121 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     3181 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     3241 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     3301 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     3361 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     3421 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     3481 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     3541 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     3601 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     3661 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     3721 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     3781 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     3841 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     3901 gcatttaaat ttccgaactc tccaaggccc tcgtcggaaa atcttcaaac ctttcgtccg
     3961 atccatcttg caggctacct ctcgaacgaa ctatcgcaag tctcttggcc ggccttgcgc
     4021 cttggctatt gcttggcagc gcctatcgcc aggtattact ccaatcccga atatccgaga
     4081 tcgggatcac ccgagagaag ttcaacctac atcctcaatc ccgatctatc cgagatccga
     4141 ggaatatcga aatcggggcg cgcctggtgt accgagaacg atcctctcag tgcgagtctc
     4201 gacgatccat atcgttgctt ggcagtcagc cagtcggaat ccagcttggg acccaggaag
     4261 tccaatcgtc agatattgta ctcaagcctg gtcacggcag cgtaccgatc tgtttaaacc
     4321 tagatattga tagtctgatc ggtcaacgta taatcgagtc ctagcttttg caaacatcta
     4381 tcaagagaca ggatcagcag gaggctttcg catgattgaa caagatggat tgcacgcagg
     4441 ttctccggcg gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     4501 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgtccggttc tttttgtcaa
     4561 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct
     4621 ggcgacgacg ggcgttcctt gcgcggctgt gctcgacgtt gtcactgaag cgggaaggga
     4681 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     4741 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     4801 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     4861 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     4921 gttcgccagg ctcaaggcgt ctatgcccga cggcgaggat ctcgtcgtga cccacggcga
     4981 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     5041 ccgtctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     5101 agagcttggc ggcgaatggg ctgaccgctt ccttgtgctt tacggtatcg ccgcgcccga
     5161 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgaccga ttctaggtgc
     5221 attggcgcag aaaaaaatgc ctgatgcgac gctgcgcgtc ttatactccc acatatgcca
     5281 gattcagcaa cggatacggc ttccccaact tgcccacttc catacgtgtc ctccttacca
     5341 gaaatttatc cttaaggtcg tttaaactcg actctggctc tatcgaatct ccgtcgtttc
     5401 gagcttacgc gaacagccgt ggcgctcatt tgctcgtcgg gcatcgaatc tcgtcagcta
     5461 tcgtcagctt acctttttgg ca
//
