LOCUS       pSF-pA-MinProm-R        4945 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector encoding a minimal promoter and Renilla luciferase, for 
            measuring the activity of transcriptional regulatory elements.
ACCESSION   .
VERSION     .
KEYWORDS    pSF-pA-MinProm-RLuc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Oxford Genetics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4945)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..89
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     terminator      complement(94..141)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    complement(158..206)
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     polyA_signal    339..387
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    401..492
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     misc_feature    493..530
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        535..597
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     RBS             689..694
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             703..1638
                     /label=RLuc
                     /note="Renilla luciferase"
     misc_feature    1661..1671
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     misc_feature    1709..1719
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    complement(1751..1872)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     terminator      1986..2033
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      2063..2199
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    2244..2315
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     rep_origin      complement(2541..3129)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     RBS             3862..3867
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             3875..4666
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      4682..4818
                     /note="rrnG terminator"
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 gcgatcgcgg ctcccgacat cttggaccat tagctccaca ggtatcttct tccctctagt
       61 ggtcataaca gcagcttcag ctacctctca attcaaaaaa cccctcaaga cccgtttaga
      121 ggccccaagg ggttatgcta tcaatcgttg cgttacacac acaaaaaacc aacacacatc
      181 catcttcgat ggatagcgat tttattatct aactgctgat cgagtgtagc cagatctatc
      241 ccaggtttac caggttcgga cttaacccac gggtctaggt agctgttcaa tccatcgttc
      301 gatccggttt tccagtcgcg acctatgtcg agtctgataa taaaatatct ttattttcat
      361 tacatctgtg tgttggtttt ttgtgtgaat cgaaagtact aacatacgct ctccatcaaa
      421 acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc cagtgcaaat gcaggtgcca
      481 gaacatttct ctgtcgacca actagtaccc cgggtagggc ccaattcgaa gtacttcgca
      541 tattaaggtg acgcgtgtgg cctcgaacac cgagcgaccc tgcagcgacc cgcttaaaag
      601 atccagccac cagatctttg tcgatcctac catccactcg acacacccgc cagcggccgc
      661 tgccaagctt ccgagctctc gaattcaaag gaggtaccca ccatgaccag caaagtgtat
      721 gatccggaac agcgcaaacg catgattacc ggcccgcagt ggtgggcgcg ctgcaaacag
      781 atgaacgtgc tggatagctt tattaactat tatgatagcg aaaaacatgc ggaaaacgcg
      841 gtgatttttc tgcatggcaa cgcggcgagc agctatctgt ggcggcatgt ggtgccgcat
      901 attgaaccag tggcgcgctg cattattccg gatctgattg gcatgggcaa aagcggcaaa
      961 agcggcaacg gcagctatcg cctgctggat cattataaat atctgaccgc gtggtttgaa
     1021 ctgctgaacc tgccgaaaaa aattattttt gtgggccatg attggggcgc gtgcctggcg
     1081 tttcattata gctatgaaca tcaggataaa attaaagcga ttgtgcacgc ggaaagcgtg
     1141 gtggatgtga ttgaaagctg ggatgaatgg ccggatattg aagaagatat tgcgctgatt
     1201 aaaagcgaag aaggcgaaaa aatggtgctg gaaaacaact tttttgtgga aaccatgctg
     1261 ccgagcaaaa ttatgcgcaa actggaaccg gaagaatttg cggcgtatct ggaaccgttt
     1321 aaagaaaaag gcgaagtgcg ccgcccgacc ctgagctggc cgcgcgaaat tccgctggtg
     1381 aaaggcggca aaccggatgt ggtccagatt gtgcgcaact ataacgcgta tctgcgcgcg
     1441 agcgatgatc tgccgaaaat gtttattgaa agcgatccgg gcttttttag caacgcgatt
     1501 gtggaaggcg cgaaaaaatt tccgaacacc gaatttgtga aagtgaaggg cctgcatttt
     1561 agccaggaag atgcgccgga tgaaatgggc aaatatatta agagctttgt ggaacgcgtg
     1621 ctgaaaaacg aacagggcta gaagttgtct cctcctgcac tgactgactg atacaatcga
     1681 tttctggatc cgcaggcctc tgctagcttg actgactgag atacagcgta ccttcagctc
     1741 acagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     1801 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     1861 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     1921 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtattggccc atctctatcg
     1981 gtatcgtagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgtgcccct
     2041 cgggccggat tgctatctac cggcattggc gcagaaaaaa atgcctgatg cgacgctgcg
     2101 cgtcttatac tcccacatat gccagattca gcaacggata cggcttcccc aacttgccca
     2161 cttccatacg tgtcctcctt accagaaatt tatccttaag gtcgtcagct atcctgcagg
     2221 cgatctctcg atttcgatca agacattcct ttaatggtct tttctggaca ccactagggg
     2281 tcagaagtag ttcatcaaac tttcttccct ccctaatctc attggttacc ttgggctatc
     2341 gaaacttaat taaccagtca agtcagctac ttggcgagat cgacttgtct gggtttcgac
     2401 tacgctcaga attgcgtcag tcaagttcga tctggtcctt gctattgcac ccgttctccg
     2461 attacgagtt tcatttaaat catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2521 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2581 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2641 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2701 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     2761 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     2821 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     2881 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2941 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     3001 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3061 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3121 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3181 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3241 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3301 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     3361 gttgcattta aatttccgaa ctctccaagg ccctcgtcgg aaaatcttca aacctttcgt
     3421 ccgatccatc ttgcaggcta cctctcgaac gaactatcgc aagtctcttg gccggccttg
     3481 cgccttggct attgcttggc agcgcctatc gccaggtatt actccaatcc cgaatatccg
     3541 agatcgggat cacccgagag aagttcaacc tacatcctca atcccgatct atccgagatc
     3601 cgaggaatat cgaaatcggg gcgcgcctgg tgtaccgaga acgatcctct cagtgcgagt
     3661 ctcgacgatc catatcgttg cttggcagtc agccagtcgg aatccagctt gggacccagg
     3721 aagtccaatc gtcagatatt gtactcaagc ctggtcacgg cagcgtaccg atctgtttaa
     3781 acctagatat tgatagtctg atcggtcaac gtataatcga gtcctagctt ttgcaaacat
     3841 ctatcaagag acaggatcag caggaggctt tcgcatgatt gaacaagatg gattgcacgc
     3901 aggttctccg gcggcttggg tggagaggct attcggctat gactgggcac aacagacaat
     3961 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgtccgg ttctttttgt
     4021 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
     4081 gctggcgacg acgggcgttc cttgcgcggc tgtgctcgac gttgtcactg aagcgggaag
     4141 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     4201 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     4261 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     4321 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     4381 actgttcgcc aggctcaagg cgtctatgcc cgacggcgag gatctcgtcg tgacccacgg
     4441 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     4501 tggccgtctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     4561 tgaagagctt ggcggcgaat gggctgaccg cttccttgtg ctttacggta tcgccgcgcc
     4621 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgac cgattctagg
     4681 tgcattggcg cagaaaaaaa tgcctgatgc gacgctgcgc gtcttatact cccacatatg
     4741 ccagattcag caacggatac ggcttcccca acttgcccac ttccatacgt gtcctcctta
     4801 ccagaaattt atccttaagg tcgtttaaac tcgactctgg ctctatcgaa tctccgtcgt
     4861 ttcgagctta cgcgaacagc cgtggcgctc atttgctcgt cgggcatcga atctcgtcag
     4921 ctatcgtcag cttacctttt tggca
//
