LOCUS       pSF-pA-MinProm-F        5659 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector encoding a minimal promoter and firefly luciferase, for 
            measuring the activity of transcriptional regulatory elements.
ACCESSION   .
VERSION     .
KEYWORDS    pSF-pA-MinProm-FLuc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Oxford Genetics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5659)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..89
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     terminator      complement(94..141)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    complement(158..206)
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     polyA_signal    339..387
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    401..492
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     misc_feature    493..530
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        535..597
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     RBS             689..694
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             703..2352
                     /label=luciferase
                     /note="firefly luciferase"
     misc_feature    2375..2385
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     misc_feature    2423..2433
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    complement(2465..2586)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     terminator      2700..2747
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      2777..2913
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    2958..3029
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     rep_origin      complement(3255..3843)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     RBS             4576..4581
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             4589..5380
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      5396..5532
                     /note="rrnG terminator"
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 gcgatcgcgg ctcccgacat cttggaccat tagctccaca ggtatcttct tccctctagt
       61 ggtcataaca gcagcttcag ctacctctca attcaaaaaa cccctcaaga cccgtttaga
      121 ggccccaagg ggttatgcta tcaatcgttg cgttacacac acaaaaaacc aacacacatc
      181 catcttcgat ggatagcgat tttattatct aactgctgat cgagtgtagc cagatctatc
      241 ccaggtttac caggttcgga cttaacccac gggtctaggt agctgttcaa tccatcgttc
      301 gatccggttt tccagtcgcg acctatgtcg agtctgataa taaaatatct ttattttcat
      361 tacatctgtg tgttggtttt ttgtgtgaat cgaaagtact aacatacgct ctccatcaaa
      421 acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc cagtgcaaat gcaggtgcca
      481 gaacatttct ctgtcgacca actagtaccc cgggtagggc ccaattcgaa gtacttcgca
      541 tattaaggtg acgcgtgtgg cctcgaacac cgagcgaccc tgcagcgacc cgcttaaaag
      601 atccagccac cagatctttg tcgatcctac catccactcg acacacccgc cagcggccgc
      661 tgccaagctt ccgagctctc gaattcaaag gaggtaccca ccatggaaga tgccaaaaac
      721 attaagaaag gcccagcgcc attctaccca ctcgaagacg ggaccgctgg cgagcagctg
      781 cataaagcca tgaagcgcta cgccctggtg cccggcacca tcgcctttac cgacgcacat
      841 atcgaggtgg acattaccta cgccgagtac ttcgagatga gcgttcggct ggcagaagct
      901 atgaagcgct atgggctgaa tacaaaccat cggatcgtgg tgtgtagcga gaatagcttg
      961 cagttcttca tgcccgtgtt gggtgccctg ttcatcggtg tggctgtggc cccagctaac
     1021 gacatctaca acgagcgcga gctgctgaac agcatgggca tcagccagcc caccgtcgta
     1081 ttcgtgagca agaaagggct gcaaaagatc ctcaacgtgc aaaagaagct accgatcata
     1141 caaaagatca tcatcatgga tagcaagacc gactaccagg gcttccaaag catgtacacc
     1201 ttcgtgactt cccatttgcc acccggcttc aacgagtacg acttcgtgcc cgagagcttc
     1261 gaccgggaca aaaccatcgc cctgatcatg aacagtagtg gcagtaccgg attgcccaag
     1321 ggcgtagccc taccgcaccg caccgcttgt gtccgattca gtcatgcccg cgaccccatc
     1381 ttcggcaacc agatcatccc cgacaccgct atcctgagcg tggtgccatt tcaccacggc
     1441 ttcggcatgt tcaccacgct gggctacttg atctgcggct ttcgggtcgt gctcatgtac
     1501 cgcttcgagg aagagctatt cttgcgcagc ttgcaagact ataagattca atctgccctg
     1561 ctggtgccca cactatttag cttcttcgct aagagcactc tcatcgacaa gtacgaccta
     1621 agcaacttgc acgagatcgc cagcggcggg gcgccgctca gcaaggaggt aggtgaggcc
     1681 gtggccaaac gcttccacct accaggcatc cgccagggct acggcctgac agaaacaacc
     1741 agcgccattc tgatcacccc cgaaggggac gacaagcctg gcgcagtagg caaggtggtg
     1801 cccttcttcg aggctaaggt ggtggacttg gacaccggta agacactggg tgtgaaccag
     1861 cgcggcgagc tgtgcgtccg tggccccatg atcatgagcg gctacgttaa caaccccgag
     1921 gctacaaacg ctctcatcga caaggacggc tggctgcata gcggcgacat cgcctactgg
     1981 gacgaggacg agcacttctt catcgtggac cggctgaaga gcctgatcaa atacaagggc
     2041 taccaggtag ccccagccga actggagagc atcctgctgc aacaccccaa catcttcgac
     2101 gccggggtcg ccggcctgcc cgacgacgat gccggcgagc tgcccgccgc agtcgtcgtg
     2161 ctggaacacg gtaaaaccat gaccgagaag gagatcgtgg actatgtggc cagccaggtt
     2221 acaaccgcca agaagctgcg cggtggtgtt gtgttcgtgg acgaggtgcc taaaggactg
     2281 accggcaagt tggacgcccg caagatccgc gagattctca ttaaggccaa gaagggcggc
     2341 aagatcgccg tctagaagtt gtctcctcct gcactgactg actgatacaa tcgatttctg
     2401 gatccgcagg cctctgctag cttgactgac tgagatacag cgtaccttca gctcacagac
     2461 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     2521 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     2581 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     2641 gttttttaaa gcaagtaaaa cctctacaaa tgtggtattg gcccatctct atcggtatcg
     2701 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgtgc ccctcgggcc
     2761 ggattgctat ctaccggcat tggcgcagaa aaaaatgcct gatgcgacgc tgcgcgtctt
     2821 atactcccac atatgccaga ttcagcaacg gatacggctt ccccaacttg cccacttcca
     2881 tacgtgtcct ccttaccaga aatttatcct taaggtcgtc agctatcctg caggcgatct
     2941 ctcgatttcg atcaagacat tcctttaatg gtcttttctg gacaccacta ggggtcagaa
     3001 gtagttcatc aaactttctt ccctccctaa tctcattggt taccttgggc tatcgaaact
     3061 taattaacca gtcaagtcag ctacttggcg agatcgactt gtctgggttt cgactacgct
     3121 cagaattgcg tcagtcaagt tcgatctggt ccttgctatt gcacccgttc tccgattacg
     3181 agtttcattt aaatcatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     3241 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     3301 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     3361 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     3421 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     3481 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     3541 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     3601 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     3661 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     3721 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3781 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     3841 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     3901 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     3961 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     4021 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgca
     4081 tttaaatttc cgaactctcc aaggccctcg tcggaaaatc ttcaaacctt tcgtccgatc
     4141 catcttgcag gctacctctc gaacgaacta tcgcaagtct cttggccggc cttgcgcctt
     4201 ggctattgct tggcagcgcc tatcgccagg tattactcca atcccgaata tccgagatcg
     4261 ggatcacccg agagaagttc aacctacatc ctcaatcccg atctatccga gatccgagga
     4321 atatcgaaat cggggcgcgc ctggtgtacc gagaacgatc ctctcagtgc gagtctcgac
     4381 gatccatatc gttgcttggc agtcagccag tcggaatcca gcttgggacc caggaagtcc
     4441 aatcgtcaga tattgtactc aagcctggtc acggcagcgt accgatctgt ttaaacctag
     4501 atattgatag tctgatcggt caacgtataa tcgagtccta gcttttgcaa acatctatca
     4561 agagacagga tcagcaggag gctttcgcat gattgaacaa gatggattgc acgcaggttc
     4621 tccggcggct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     4681 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgt ccggttcttt ttgtcaagac
     4741 cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc
     4801 gacgacgggc gttccttgcg cggctgtgct cgacgttgtc actgaagcgg gaagggactg
     4861 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     4921 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     4981 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     5041 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     5101 cgccaggctc aaggcgtcta tgcccgacgg cgaggatctc gtcgtgaccc acggcgatgc
     5161 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     5221 tctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     5281 gcttggcggc gaatgggctg accgcttcct tgtgctttac ggtatcgccg cgcccgattc
     5341 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaccgattc taggtgcatt
     5401 ggcgcagaaa aaaatgcctg atgcgacgct gcgcgtctta tactcccaca tatgccagat
     5461 tcagcaacgg atacggcttc cccaacttgc ccacttccat acgtgtcctc cttaccagaa
     5521 atttatcctt aaggtcgttt aaactcgact ctggctctat cgaatctccg tcgtttcgag
     5581 cttacgcgaa cagccgtggc gctcatttgc tcgtcgggca tcgaatctcg tcagctatcg
     5641 tcagcttacc tttttggca
//
