LOCUS       pSF-pA-CMVe-RLuc        5259 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector encoding the CMV enhancer and Renilla luciferase, for 
            low-background measurements of promoter activity.
ACCESSION   .
VERSION     .
KEYWORDS    pSF-pA-CMVe-RLuc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Oxford Genetics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5259)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..89
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     terminator      complement(94..141)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    complement(158..206)
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     enhancer        298..601
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    731..779
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    793..884
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     misc_feature    885..922
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     RBS             1003..1008
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             1017..1952
                     /label=RLuc
                     /note="Renilla luciferase"
     misc_feature    1975..1985
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     misc_feature    2023..2033
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    complement(2065..2186)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     terminator      2300..2347
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      2377..2513
                     /note="rrnG terminator"
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    2558..2629
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     rep_origin      complement(2855..3443)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     RBS             4176..4181
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             4189..4980
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      4996..5132
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 gcgatcgcgg ctcccgacat cttggaccat tagctccaca ggtatcttct tccctctagt
       61 ggtcataaca gcagcttcag ctacctctca attcaaaaaa cccctcaaga cccgtttaga
      121 ggccccaagg ggttatgcta tcaatcgttg cgttacacac acaaaaaacc aacacacatc
      181 catcttcgat ggatagcgat tttattatct aactgctgat cgagtgtagc cagatcttag
      241 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      301 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      361 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      421 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      481 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      541 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      601 gctgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      661 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggctcgtcg cgacctttgt
      721 cgagtctgat aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga
      781 atcgaaagta ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa
      841 ataggctgtc cccagtgcaa atgcaggtgc cagaacattt ctctgtcgac caactagtac
      901 cccgggtagg gcccaattcg aaaccagatc tttgtcgatc ctaccatcca ctcgacacac
      961 ccgccagcgg ccgctgccaa gcttccgagc tctcgaattc aaaggaggta cccaccatga
     1021 ccagcaaagt gtatgatccg gaacagcgca aacgcatgat taccggcccg cagtggtggg
     1081 cgcgctgcaa acagatgaac gtgctggata gctttattaa ctattatgat agcgaaaaac
     1141 atgcggaaaa cgcggtgatt tttctgcatg gcaacgcggc gagcagctat ctgtggcggc
     1201 atgtggtgcc gcatattgaa ccagtggcgc gctgcattat tccggatctg attggcatgg
     1261 gcaaaagcgg caaaagcggc aacggcagct atcgcctgct ggatcattat aaatatctga
     1321 ccgcgtggtt tgaactgctg aacctgccga aaaaaattat ttttgtgggc catgattggg
     1381 gcgcgtgcct ggcgtttcat tatagctatg aacatcagga taaaattaaa gcgattgtgc
     1441 acgcggaaag cgtggtggat gtgattgaaa gctgggatga atggccggat attgaagaag
     1501 atattgcgct gattaaaagc gaagaaggcg aaaaaatggt gctggaaaac aacttttttg
     1561 tggaaaccat gctgccgagc aaaattatgc gcaaactgga accggaagaa tttgcggcgt
     1621 atctggaacc gtttaaagaa aaaggcgaag tgcgccgccc gaccctgagc tggccgcgcg
     1681 aaattccgct ggtgaaaggc ggcaaaccgg atgtggtcca gattgtgcgc aactataacg
     1741 cgtatctgcg cgcgagcgat gatctgccga aaatgtttat tgaaagcgat ccgggctttt
     1801 ttagcaacgc gattgtggaa ggcgcgaaaa aatttccgaa caccgaattt gtgaaagtga
     1861 agggcctgca ttttagccag gaagatgcgc cggatgaaat gggcaaatat attaagagct
     1921 ttgtggaacg cgtgctgaaa aacgaacagg gctagaagtt gtctcctcct gcactgactg
     1981 actgatacaa tcgatttctg gatccgcagg cctctgctag cttgactgac tgagatacag
     2041 cgtaccttca gctcacagac atgataagat acattgatga gtttggacaa accacaacta
     2101 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     2161 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     2221 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtattg
     2281 gcccatctct atcggtatcg tagcataacc ccttggggcc tctaaacggg tcttgagggg
     2341 ttttttgtgc ccctcgggcc ggattgctat ctaccggcat tggcgcagaa aaaaatgcct
     2401 gatgcgacgc tgcgcgtctt atactcccac atatgccaga ttcagcaacg gatacggctt
     2461 ccccaacttg cccacttcca tacgtgtcct ccttaccaga aatttatcct taaggtcgtc
     2521 agctatcctg caggcgatct ctcgatttcg atcaagacat tcctttaatg gtcttttctg
     2581 gacaccacta ggggtcagaa gtagttcatc aaactttctt ccctccctaa tctcattggt
     2641 taccttgggc tatcgaaact taattaacca gtcaagtcag ctacttggcg agatcgactt
     2701 gtctgggttt cgactacgct cagaattgcg tcagtcaagt tcgatctggt ccttgctatt
     2761 gcacccgttc tccgattacg agtttcattt aaatcatgtg agcaaaaggc cagcaaaagg
     2821 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     2881 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     2941 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3001 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3061 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3121 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3181 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3241 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     3301 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3361 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3421 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3481 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     3541 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     3601 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     3661 ttcgttcatc catagttgca tttaaatttc cgaactctcc aaggccctcg tcggaaaatc
     3721 ttcaaacctt tcgtccgatc catcttgcag gctacctctc gaacgaacta tcgcaagtct
     3781 cttggccggc cttgcgcctt ggctattgct tggcagcgcc tatcgccagg tattactcca
     3841 atcccgaata tccgagatcg ggatcacccg agagaagttc aacctacatc ctcaatcccg
     3901 atctatccga gatccgagga atatcgaaat cggggcgcgc ctggtgtacc gagaacgatc
     3961 ctctcagtgc gagtctcgac gatccatatc gttgcttggc agtcagccag tcggaatcca
     4021 gcttgggacc caggaagtcc aatcgtcaga tattgtactc aagcctggtc acggcagcgt
     4081 accgatctgt ttaaacctag atattgatag tctgatcggt caacgtataa tcgagtccta
     4141 gcttttgcaa acatctatca agagacagga tcagcaggag gctttcgcat gattgaacaa
     4201 gatggattgc acgcaggttc tccggcggct tgggtggaga ggctattcgg ctatgactgg
     4261 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgt
     4321 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca
     4381 gcgcggctat cgtggctggc gacgacgggc gttccttgcg cggctgtgct cgacgttgtc
     4441 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     4501 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     4561 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     4621 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     4681 ctcgcgccag ccgaactgtt cgccaggctc aaggcgtcta tgcccgacgg cgaggatctc
     4741 gtcgtgaccc acggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     4801 ggattcatcg actgtggccg tctgggtgtg gcggaccgct atcaggacat agcgttggct
     4861 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct tgtgctttac
     4921 ggtatcgccg cgcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     4981 tgaccgattc taggtgcatt ggcgcagaaa aaaatgcctg atgcgacgct gcgcgtctta
     5041 tactcccaca tatgccagat tcagcaacgg atacggcttc cccaacttgc ccacttccat
     5101 acgtgtcctc cttaccagaa atttatcctt aaggtcgttt aaactcgact ctggctctat
     5161 cgaatctccg tcgtttcgag cttacgcgaa cagccgtggc gctcatttgc tcgtcgggca
     5221 tcgaatctcg tcagctatcg tcagcttacc tttttggca
//
