LOCUS       pSF-pA-CMVe-FLuc        5973 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector encoding the CMV enhancer and firefly luciferase, for 
            low-background measurements of promoter activity.
ACCESSION   .
VERSION     .
KEYWORDS    pSF-pA-CMVe-FLuc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5973)
  AUTHORS   Oxford Genetics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5973)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5973
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..89
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     terminator      complement(94..141)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    complement(158..206)
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     enhancer        298..601
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    731..779
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    793..884
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     misc_feature    885..922
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     RBS             1003..1008
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             1017..2666
                     /label=luciferase
                     /note="firefly luciferase"
     misc_feature    2689..2699
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     misc_feature    2737..2747
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    complement(2779..2900)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     terminator      3014..3061
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      3091..3227
                     /note="rrnG terminator"
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    3272..3343
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     rep_origin      complement(3569..4157)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     RBS             4890..4895
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             4903..5694
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      5710..5846
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 gcgatcgcgg ctcccgacat cttggaccat tagctccaca ggtatcttct tccctctagt
       61 ggtcataaca gcagcttcag ctacctctca attcaaaaaa cccctcaaga cccgtttaga
      121 ggccccaagg ggttatgcta tcaatcgttg cgttacacac acaaaaaacc aacacacatc
      181 catcttcgat ggatagcgat tttattatct aactgctgat cgagtgtagc cagatcttag
      241 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      301 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      361 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      421 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      481 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      541 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      601 gctgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      661 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggctcgtcg cgacctttgt
      721 cgagtctgat aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga
      781 atcgaaagta ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa
      841 ataggctgtc cccagtgcaa atgcaggtgc cagaacattt ctctgtcgac caactagtac
      901 cccgggtagg gcccaattcg aaaccagatc tttgtcgatc ctaccatcca ctcgacacac
      961 ccgccagcgg ccgctgccaa gcttccgagc tctcgaattc aaaggaggta cccaccatgg
     1021 aagatgccaa aaacattaag aaaggcccag cgccattcta cccactcgaa gacgggaccg
     1081 ctggcgagca gctgcataaa gccatgaagc gctacgccct ggtgcccggc accatcgcct
     1141 ttaccgacgc acatatcgag gtggacatta cctacgccga gtacttcgag atgagcgttc
     1201 ggctggcaga agctatgaag cgctatgggc tgaatacaaa ccatcggatc gtggtgtgta
     1261 gcgagaatag cttgcagttc ttcatgcccg tgttgggtgc cctgttcatc ggtgtggctg
     1321 tggccccagc taacgacatc tacaacgagc gcgagctgct gaacagcatg ggcatcagcc
     1381 agcccaccgt cgtattcgtg agcaagaaag ggctgcaaaa gatcctcaac gtgcaaaaga
     1441 agctaccgat catacaaaag atcatcatca tggatagcaa gaccgactac cagggcttcc
     1501 aaagcatgta caccttcgtg acttcccatt tgccacccgg cttcaacgag tacgacttcg
     1561 tgcccgagag cttcgaccgg gacaaaacca tcgccctgat catgaacagt agtggcagta
     1621 ccggattgcc caagggcgta gccctaccgc accgcaccgc ttgtgtccga ttcagtcatg
     1681 cccgcgaccc catcttcggc aaccagatca tccccgacac cgctatcctg agcgtggtgc
     1741 catttcacca cggcttcggc atgttcacca cgctgggcta cttgatctgc ggctttcggg
     1801 tcgtgctcat gtaccgcttc gaggaagagc tattcttgcg cagcttgcaa gactataaga
     1861 ttcaatctgc cctgctggtg cccacactat ttagcttctt cgctaagagc actctcatcg
     1921 acaagtacga cctaagcaac ttgcacgaga tcgccagcgg cggggcgccg ctcagcaagg
     1981 aggtaggtga ggccgtggcc aaacgcttcc acctaccagg catccgccag ggctacggcc
     2041 tgacagaaac aaccagcgcc attctgatca cccccgaagg ggacgacaag cctggcgcag
     2101 taggcaaggt ggtgcccttc ttcgaggcta aggtggtgga cttggacacc ggtaagacac
     2161 tgggtgtgaa ccagcgcggc gagctgtgcg tccgtggccc catgatcatg agcggctacg
     2221 ttaacaaccc cgaggctaca aacgctctca tcgacaagga cggctggctg catagcggcg
     2281 acatcgccta ctgggacgag gacgagcact tcttcatcgt ggaccggctg aagagcctga
     2341 tcaaatacaa gggctaccag gtagccccag ccgaactgga gagcatcctg ctgcaacacc
     2401 ccaacatctt cgacgccggg gtcgccggcc tgcccgacga cgatgccggc gagctgcccg
     2461 ccgcagtcgt cgtgctggaa cacggtaaaa ccatgaccga gaaggagatc gtggactatg
     2521 tggccagcca ggttacaacc gccaagaagc tgcgcggtgg tgttgtgttc gtggacgagg
     2581 tgcctaaagg actgaccggc aagttggacg cccgcaagat ccgcgagatt ctcattaagg
     2641 ccaagaaggg cggcaagatc gccgtctaga agttgtctcc tcctgcactg actgactgat
     2701 acaatcgatt tctggatccg caggcctctg ctagcttgac tgactgagat acagcgtacc
     2761 ttcagctcac agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     2821 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     2881 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     2941 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt attggcccat
     3001 ctctatcggt atcgtagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
     3061 gtgcccctcg ggccggattg ctatctaccg gcattggcgc agaaaaaaat gcctgatgcg
     3121 acgctgcgcg tcttatactc ccacatatgc cagattcagc aacggatacg gcttccccaa
     3181 cttgcccact tccatacgtg tcctccttac cagaaattta tccttaaggt cgtcagctat
     3241 cctgcaggcg atctctcgat ttcgatcaag acattccttt aatggtcttt tctggacacc
     3301 actaggggtc agaagtagtt catcaaactt tcttccctcc ctaatctcat tggttacctt
     3361 gggctatcga aacttaatta accagtcaag tcagctactt ggcgagatcg acttgtctgg
     3421 gtttcgacta cgctcagaat tgcgtcagtc aagttcgatc tggtccttgc tattgcaccc
     3481 gttctccgat tacgagtttc atttaaatca tgtgagcaaa aggccagcaa aaggccagga
     3541 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     3601 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     3661 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     3721 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     3781 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     3841 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     3901 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     3961 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     4021 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     4081 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     4141 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     4201 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     4261 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     4321 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     4381 catccatagt tgcatttaaa tttccgaact ctccaaggcc ctcgtcggaa aatcttcaaa
     4441 cctttcgtcc gatccatctt gcaggctacc tctcgaacga actatcgcaa gtctcttggc
     4501 cggccttgcg ccttggctat tgcttggcag cgcctatcgc caggtattac tccaatcccg
     4561 aatatccgag atcgggatca cccgagagaa gttcaaccta catcctcaat cccgatctat
     4621 ccgagatccg aggaatatcg aaatcggggc gcgcctggtg taccgagaac gatcctctca
     4681 gtgcgagtct cgacgatcca tatcgttgct tggcagtcag ccagtcggaa tccagcttgg
     4741 gacccaggaa gtccaatcgt cagatattgt actcaagcct ggtcacggca gcgtaccgat
     4801 ctgtttaaac ctagatattg atagtctgat cggtcaacgt ataatcgagt cctagctttt
     4861 gcaaacatct atcaagagac aggatcagca ggaggctttc gcatgattga acaagatgga
     4921 ttgcacgcag gttctccggc ggcttgggtg gagaggctat tcggctatga ctgggcacaa
     4981 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgtccggtt
     5041 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg
     5101 ctatcgtggc tggcgacgac gggcgttcct tgcgcggctg tgctcgacgt tgtcactgaa
     5161 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     5221 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     5281 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     5341 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     5401 ccagccgaac tgttcgccag gctcaaggcg tctatgcccg acggcgagga tctcgtcgtg
     5461 acccacggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     5521 atcgactgtg gccgtctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     5581 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tccttgtgct ttacggtatc
     5641 gccgcgcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaccg
     5701 attctaggtg cattggcgca gaaaaaaatg cctgatgcga cgctgcgcgt cttatactcc
     5761 cacatatgcc agattcagca acggatacgg cttccccaac ttgcccactt ccatacgtgt
     5821 cctccttacc agaaatttat ccttaaggtc gtttaaactc gactctggct ctatcgaatc
     5881 tccgtcgttt cgagcttacg cgaacagccg tggcgctcat ttgctcgtcg ggcatcgaat
     5941 ctcgtcagct atcgtcagct tacctttttg gca
//
