LOCUS       pSF-RecA-FLuc.        5475 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for expressing firefly luciferase from a strong 
            constitutive recA promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pSF-RecA-FLuc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Oxford Genetics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5475)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5475
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..89
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     terminator      complement(94..141)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    complement(158..206)
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     promoter        359..427
                     /label=recA (Delta-LexA) promoter
                     /note="constitutively active E. coli recA promoter lacking
                     the LexA binding site (Brent and Ptashne, 1981)"
     RBS             505..510
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             519..2168
                     /label=luciferase
                     /note="firefly luciferase"
     misc_feature    2191..2201
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     misc_feature    2239..2249
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    complement(2281..2402)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     terminator      2516..2563
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      2593..2729
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    2774..2845
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     rep_origin      complement(3071..3659)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     RBS             4392..4397
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             4405..5196
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      5212..5348
                     /note="rrnG terminator"
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 gcgatcgcgg ctcccgacat cttggaccat tagctccaca ggtatcttct tccctctagt
       61 ggtcataaca gcagcttcag ctacctctca attcaaaaaa cccctcaaga cccgtttaga
      121 ggccccaagg ggttatgcta tcaatcgttg cgttacacac acaaaaaacc aacacacatc
      181 catcttcgat ggatagcgat tttattatct aactgctgat cgagtgtagc cagatctaag
      241 ctgttgtgac cgcttgctct agccagctat cgagttgtga accgatccat ctagcaattg
      301 gtctcgatct agcgataggc ttcgatctag ctatgtagaa acgccgtgtg ctcgatcgct
      361 tgataaggtc cacgtagctg ctataattgc ttcaacagaa catattgact atccggtatt
      421 acccggcaga tctttgtcga tcctaccatc cactcgacac acccgccagc ggccgctgcc
      481 aagcttccga gctctcgaat tcaaaggagg tacccaccat ggaagatgcc aaaaacatta
      541 agaaaggccc agcgccattc tacccactcg aagacgggac cgctggcgag cagctgcata
      601 aagccatgaa gcgctacgcc ctggtgcccg gcaccatcgc ctttaccgac gcacatatcg
      661 aggtggacat tacctacgcc gagtacttcg agatgagcgt tcggctggca gaagctatga
      721 agcgctatgg gctgaataca aaccatcgga tcgtggtgtg tagcgagaat agcttgcagt
      781 tcttcatgcc cgtgttgggt gccctgttca tcggtgtggc tgtggcccca gctaacgaca
      841 tctacaacga gcgcgagctg ctgaacagca tgggcatcag ccagcccacc gtcgtattcg
      901 tgagcaagaa agggctgcaa aagatcctca acgtgcaaaa gaagctaccg atcatacaaa
      961 agatcatcat catggatagc aagaccgact accagggctt ccaaagcatg tacaccttcg
     1021 tgacttccca tttgccaccc ggcttcaacg agtacgactt cgtgcccgag agcttcgacc
     1081 gggacaaaac catcgccctg atcatgaaca gtagtggcag taccggattg cccaagggcg
     1141 tagccctacc gcaccgcacc gcttgtgtcc gattcagtca tgcccgcgac cccatcttcg
     1201 gcaaccagat catccccgac accgctatcc tgagcgtggt gccatttcac cacggcttcg
     1261 gcatgttcac cacgctgggc tacttgatct gcggctttcg ggtcgtgctc atgtaccgct
     1321 tcgaggaaga gctattcttg cgcagcttgc aagactataa gattcaatct gccctgctgg
     1381 tgcccacact atttagcttc ttcgctaaga gcactctcat cgacaagtac gacctaagca
     1441 acttgcacga gatcgccagc ggcggggcgc cgctcagcaa ggaggtaggt gaggccgtgg
     1501 ccaaacgctt ccacctacca ggcatccgcc agggctacgg cctgacagaa acaaccagcg
     1561 ccattctgat cacccccgaa ggggacgaca agcctggcgc agtaggcaag gtggtgccct
     1621 tcttcgaggc taaggtggtg gacttggaca ccggtaagac actgggtgtg aaccagcgcg
     1681 gcgagctgtg cgtccgtggc cccatgatca tgagcggcta cgttaacaac cccgaggcta
     1741 caaacgctct catcgacaag gacggctggc tgcatagcgg cgacatcgcc tactgggacg
     1801 aggacgagca cttcttcatc gtggaccggc tgaagagcct gatcaaatac aagggctacc
     1861 aggtagcccc agccgaactg gagagcatcc tgctgcaaca ccccaacatc ttcgacgccg
     1921 gggtcgccgg cctgcccgac gacgatgccg gcgagctgcc cgccgcagtc gtcgtgctgg
     1981 aacacggtaa aaccatgacc gagaaggaga tcgtggacta tgtggccagc caggttacaa
     2041 ccgccaagaa gctgcgcggt ggtgttgtgt tcgtggacga ggtgcctaaa ggactgaccg
     2101 gcaagttgga cgcccgcaag atccgcgaga ttctcattaa ggccaagaag ggcggcaaga
     2161 tcgccgtcta gaagttgtct cctcctgcac tgactgactg atacaatcga tttctggatc
     2221 cgcaggcctc tgctagcttg actgactgag atacagcgta ccttcagctc acagacatga
     2281 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     2341 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     2401 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     2461 tttaaagcaa gtaaaacctc tacaaatgtg gtattggccc atctctatcg gtatcgtagc
     2521 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgtgcccct cgggccggat
     2581 tgctatctac cggcattggc gcagaaaaaa atgcctgatg cgacgctgcg cgtcttatac
     2641 tcccacatat gccagattca gcaacggata cggcttcccc aacttgccca cttccatacg
     2701 tgtcctcctt accagaaatt tatccttaag gtcgtcagct atcctgcagg cgatctctcg
     2761 atttcgatca agacattcct ttaatggtct tttctggaca ccactagggg tcagaagtag
     2821 ttcatcaaac tttcttccct ccctaatctc attggttacc ttgggctatc gaaacttaat
     2881 taaccagtca agtcagctac ttggcgagat cgacttgtct gggtttcgac tacgctcaga
     2941 attgcgtcag tcaagttcga tctggtcctt gctattgcac ccgttctccg attacgagtt
     3001 tcatttaaat catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     3061 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     3121 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     3181 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     3241 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     3301 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     3361 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     3421 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     3481 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     3541 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     3601 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     3661 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     3721 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     3781 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     3841 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcattta
     3901 aatttccgaa ctctccaagg ccctcgtcgg aaaatcttca aacctttcgt ccgatccatc
     3961 ttgcaggcta cctctcgaac gaactatcgc aagtctcttg gccggccttg cgccttggct
     4021 attgcttggc agcgcctatc gccaggtatt actccaatcc cgaatatccg agatcgggat
     4081 cacccgagag aagttcaacc tacatcctca atcccgatct atccgagatc cgaggaatat
     4141 cgaaatcggg gcgcgcctgg tgtaccgaga acgatcctct cagtgcgagt ctcgacgatc
     4201 catatcgttg cttggcagtc agccagtcgg aatccagctt gggacccagg aagtccaatc
     4261 gtcagatatt gtactcaagc ctggtcacgg cagcgtaccg atctgtttaa acctagatat
     4321 tgatagtctg atcggtcaac gtataatcga gtcctagctt ttgcaaacat ctatcaagag
     4381 acaggatcag caggaggctt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     4441 gcggcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     4501 gatgccgccg tgttccggct gtcagcgcag gggcgtccgg ttctttttgt caagaccgac
     4561 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggcgacg
     4621 acgggcgttc cttgcgcggc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     4681 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     4741 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     4801 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     4861 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     4921 aggctcaagg cgtctatgcc cgacggcgag gatctcgtcg tgacccacgg cgatgcctgc
     4981 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccgtctg
     5041 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     5101 ggcggcgaat gggctgaccg cttccttgtg ctttacggta tcgccgcgcc cgattcgcag
     5161 cgcatcgcct tctatcgcct tcttgacgag ttcttctgac cgattctagg tgcattggcg
     5221 cagaaaaaaa tgcctgatgc gacgctgcgc gtcttatact cccacatatg ccagattcag
     5281 caacggatac ggcttcccca acttgcccac ttccatacgt gtcctcctta ccagaaattt
     5341 atccttaagg tcgtttaaac tcgactctgg ctctatcgaa tctccgtcgt ttcgagctta
     5401 cgcgaacagc cgtggcgctc atttgctcgt cgggcatcga atctcgtcag ctatcgtcag
     5461 cttacctttt tggca
//
