LOCUS       pSF-PromMCS-RLuc        4710 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for measuring promoter activity using Renilla luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pSF-PromMCS-RLuc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Oxford Genetics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     No poly(A) signal is present upstream of the MCS, so some background
            luciferase transcription may be observed.
FEATURES             Location/Qualifiers
     source          1..4710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..89
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     terminator      complement(94..141)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    complement(158..206)
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    245..336
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     misc_feature    337..374
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     RBS             454..459
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             468..1403
                     /label=RLuc
                     /note="Renilla luciferase"
     misc_feature    1426..1436
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     misc_feature    1474..1484
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    complement(1516..1637)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     terminator      1751..1798
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      1828..1964
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    2009..2080
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     rep_origin      complement(2306..2894)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     RBS             3627..3632
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             3640..4431
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      4447..4583
                     /note="rrnG terminator"
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 gcgatcgcgg ctcccgacat cttggaccat tagctccaca ggtatcttct tccctctagt
       61 ggtcataaca gcagcttcag ctacctctca attcaaaaaa cccctcaaga cccgtttaga
      121 ggccccaagg ggttatgcta tcaatcgttg cgttacacac acaaaaaacc aacacacatc
      181 catcttcgat ggatagcgat tttattatct aactgctgat cgagtgtagc cagatctaag
      241 tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg
      301 tccccagtgc aaatgcaggt gccagaacat ttctctgtcg accaactagt accccgggta
      361 gggcccaatt cgaagtagat ctttgtcgat cctaccatcc actcgacaca cccgccagcg
      421 gccgctgcca agcttccgag ctctcgaatt caaaggaggt acccaccatg accagcaaag
      481 tgtatgatcc ggaacagcgc aaacgcatga ttaccggccc gcagtggtgg gcgcgctgca
      541 aacagatgaa cgtgctggat agctttatta actattatga tagcgaaaaa catgcggaaa
      601 acgcggtgat ttttctgcat ggcaacgcgg cgagcagcta tctgtggcgg catgtggtgc
      661 cgcatattga accagtggcg cgctgcatta ttccggatct gattggcatg ggcaaaagcg
      721 gcaaaagcgg caacggcagc tatcgcctgc tggatcatta taaatatctg accgcgtggt
      781 ttgaactgct gaacctgccg aaaaaaatta tttttgtggg ccatgattgg ggcgcgtgcc
      841 tggcgtttca ttatagctat gaacatcagg ataaaattaa agcgattgtg cacgcggaaa
      901 gcgtggtgga tgtgattgaa agctgggatg aatggccgga tattgaagaa gatattgcgc
      961 tgattaaaag cgaagaaggc gaaaaaatgg tgctggaaaa caactttttt gtggaaacca
     1021 tgctgccgag caaaattatg cgcaaactgg aaccggaaga atttgcggcg tatctggaac
     1081 cgtttaaaga aaaaggcgaa gtgcgccgcc cgaccctgag ctggccgcgc gaaattccgc
     1141 tggtgaaagg cggcaaaccg gatgtggtcc agattgtgcg caactataac gcgtatctgc
     1201 gcgcgagcga tgatctgccg aaaatgttta ttgaaagcga tccgggcttt tttagcaacg
     1261 cgattgtgga aggcgcgaaa aaatttccga acaccgaatt tgtgaaagtg aagggcctgc
     1321 attttagcca ggaagatgcg ccggatgaaa tgggcaaata tattaagagc tttgtggaac
     1381 gcgtgctgaa aaacgaacag ggctagaagt tgtctcctcc tgcactgact gactgataca
     1441 atcgatttct ggatccgcag gcctctgcta gcttgactga ctgagataca gcgtaccttc
     1501 agctcacaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     1561 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     1621 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     1681 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatt ggcccatctc
     1741 tatcggtatc gtagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgtg
     1801 cccctcgggc cggattgcta tctaccggca ttggcgcaga aaaaaatgcc tgatgcgacg
     1861 ctgcgcgtct tatactccca catatgccag attcagcaac ggatacggct tccccaactt
     1921 gcccacttcc atacgtgtcc tccttaccag aaatttatcc ttaaggtcgt cagctatcct
     1981 gcaggcgatc tctcgatttc gatcaagaca ttcctttaat ggtcttttct ggacaccact
     2041 aggggtcaga agtagttcat caaactttct tccctcccta atctcattgg ttaccttggg
     2101 ctatcgaaac ttaattaacc agtcaagtca gctacttggc gagatcgact tgtctgggtt
     2161 tcgactacgc tcagaattgc gtcagtcaag ttcgatctgg tccttgctat tgcacccgtt
     2221 ctccgattac gagtttcatt taaatcatgt gagcaaaagg ccagcaaaag gccaggaacc
     2281 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     2341 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     2401 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     2461 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     2521 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     2581 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     2641 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     2701 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     2761 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2821 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2881 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     2941 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3001 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     3061 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     3121 ccatagttgc atttaaattt ccgaactctc caaggccctc gtcggaaaat cttcaaacct
     3181 ttcgtccgat ccatcttgca ggctacctct cgaacgaact atcgcaagtc tcttggccgg
     3241 ccttgcgcct tggctattgc ttggcagcgc ctatcgccag gtattactcc aatcccgaat
     3301 atccgagatc gggatcaccc gagagaagtt caacctacat cctcaatccc gatctatccg
     3361 agatccgagg aatatcgaaa tcggggcgcg cctggtgtac cgagaacgat cctctcagtg
     3421 cgagtctcga cgatccatat cgttgcttgg cagtcagcca gtcggaatcc agcttgggac
     3481 ccaggaagtc caatcgtcag atattgtact caagcctggt cacggcagcg taccgatctg
     3541 tttaaaccta gatattgata gtctgatcgg tcaacgtata atcgagtcct agcttttgca
     3601 aacatctatc aagagacagg atcagcagga ggctttcgca tgattgaaca agatggattg
     3661 cacgcaggtt ctccggcggc ttgggtggag aggctattcg gctatgactg ggcacaacag
     3721 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg tccggttctt
     3781 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta
     3841 tcgtggctgg cgacgacggg cgttccttgc gcggctgtgc tcgacgttgt cactgaagcg
     3901 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     3961 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     4021 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     4081 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     4141 gccgaactgt tcgccaggct caaggcgtct atgcccgacg gcgaggatct cgtcgtgacc
     4201 cacggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     4261 gactgtggcc gtctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     4321 attgctgaag agcttggcgg cgaatgggct gaccgcttcc ttgtgcttta cggtatcgcc
     4381 gcgcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaccgatt
     4441 ctaggtgcat tggcgcagaa aaaaatgcct gatgcgacgc tgcgcgtctt atactcccac
     4501 atatgccaga ttcagcaacg gatacggctt ccccaacttg cccacttcca tacgtgtcct
     4561 ccttaccaga aatttatcct taaggtcgtt taaactcgac tctggctcta tcgaatctcc
     4621 gtcgtttcga gcttacgcga acagccgtgg cgctcatttg ctcgtcgggc atcgaatctc
     4681 gtcagctatc gtcagcttac ctttttggca
//
