LOCUS       pSF-PromMCS-FLuc        5424 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for measuring promoter activity using firefly luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pSF-PromMCS-FLuc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Oxford Genetics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5424)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     No poly(A) signal is present upstream of the MCS, so some background
            luciferase transcription may be observed.
FEATURES             Location/Qualifiers
     source          1..5424
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..89
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     terminator      complement(94..141)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    complement(158..206)
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    245..336
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     misc_feature    337..374
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     RBS             454..459
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             468..2117
                     /label=luciferase
                     /note="firefly luciferase"
     misc_feature    2140..2150
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     misc_feature    2188..2198
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    complement(2230..2351)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     terminator      2465..2512
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      2542..2678
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    2723..2794
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     rep_origin      complement(3020..3608)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     RBS             4341..4346
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             4354..5145
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      5161..5297
                     /note="rrnG terminator"
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 gcgatcgcgg ctcccgacat cttggaccat tagctccaca ggtatcttct tccctctagt
       61 ggtcataaca gcagcttcag ctacctctca attcaaaaaa cccctcaaga cccgtttaga
      121 ggccccaagg ggttatgcta tcaatcgttg cgttacacac acaaaaaacc aacacacatc
      181 catcttcgat ggatagcgat tttattatct aactgctgat cgagtgtagc cagatctaag
      241 tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg
      301 tccccagtgc aaatgcaggt gccagaacat ttctctgtcg accaactagt accccgggta
      361 gggcccaatt cgaagtagat ctttgtcgat cctaccatcc actcgacaca cccgccagcg
      421 gccgctgcca agcttccgag ctctcgaatt caaaggaggt acccaccatg gaagatgcca
      481 aaaacattaa gaaaggccca gcgccattct acccactcga agacgggacc gctggcgagc
      541 agctgcataa agccatgaag cgctacgccc tggtgcccgg caccatcgcc tttaccgacg
      601 cacatatcga ggtggacatt acctacgccg agtacttcga gatgagcgtt cggctggcag
      661 aagctatgaa gcgctatggg ctgaatacaa accatcggat cgtggtgtgt agcgagaata
      721 gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat cggtgtggct gtggccccag
      781 ctaacgacat ctacaacgag cgcgagctgc tgaacagcat gggcatcagc cagcccaccg
      841 tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa cgtgcaaaag aagctaccga
      901 tcatacaaaa gatcatcatc atggatagca agaccgacta ccagggcttc caaagcatgt
      961 acaccttcgt gacttcccat ttgccacccg gcttcaacga gtacgacttc gtgcccgaga
     1021 gcttcgaccg ggacaaaacc atcgccctga tcatgaacag tagtggcagt accggattgc
     1081 ccaagggcgt agccctaccg caccgcaccg cttgtgtccg attcagtcat gcccgcgacc
     1141 ccatcttcgg caaccagatc atccccgaca ccgctatcct gagcgtggtg ccatttcacc
     1201 acggcttcgg catgttcacc acgctgggct acttgatctg cggctttcgg gtcgtgctca
     1261 tgtaccgctt cgaggaagag ctattcttgc gcagcttgca agactataag attcaatctg
     1321 ccctgctggt gcccacacta tttagcttct tcgctaagag cactctcatc gacaagtacg
     1381 acctaagcaa cttgcacgag atcgccagcg gcggggcgcc gctcagcaag gaggtaggtg
     1441 aggccgtggc caaacgcttc cacctaccag gcatccgcca gggctacggc ctgacagaaa
     1501 caaccagcgc cattctgatc acccccgaag gggacgacaa gcctggcgca gtaggcaagg
     1561 tggtgccctt cttcgaggct aaggtggtgg acttggacac cggtaagaca ctgggtgtga
     1621 accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat gagcggctac gttaacaacc
     1681 ccgaggctac aaacgctctc atcgacaagg acggctggct gcatagcggc gacatcgcct
     1741 actgggacga ggacgagcac ttcttcatcg tggaccggct gaagagcctg atcaaataca
     1801 agggctacca ggtagcccca gccgaactgg agagcatcct gctgcaacac cccaacatct
     1861 tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg cgagctgccc gccgcagtcg
     1921 tcgtgctgga acacggtaaa accatgaccg agaaggagat cgtggactat gtggccagcc
     1981 aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt cgtggacgag gtgcctaaag
     2041 gactgaccgg caagttggac gcccgcaaga tccgcgagat tctcattaag gccaagaagg
     2101 gcggcaagat cgccgtctag aagttgtctc ctcctgcact gactgactga tacaatcgat
     2161 ttctggatcc gcaggcctct gctagcttga ctgactgaga tacagcgtac cttcagctca
     2221 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     2281 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     2341 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
     2401 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tattggccca tctctatcgg
     2461 tatcgtagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgtgcccctc
     2521 gggccggatt gctatctacc ggcattggcg cagaaaaaaa tgcctgatgc gacgctgcgc
     2581 gtcttatact cccacatatg ccagattcag caacggatac ggcttcccca acttgcccac
     2641 ttccatacgt gtcctcctta ccagaaattt atccttaagg tcgtcagcta tcctgcaggc
     2701 gatctctcga tttcgatcaa gacattcctt taatggtctt ttctggacac cactaggggt
     2761 cagaagtagt tcatcaaact ttcttccctc cctaatctca ttggttacct tgggctatcg
     2821 aaacttaatt aaccagtcaa gtcagctact tggcgagatc gacttgtctg ggtttcgact
     2881 acgctcagaa ttgcgtcagt caagttcgat ctggtccttg ctattgcacc cgttctccga
     2941 ttacgagttt catttaaatc atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3001 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3061 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3121 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     3181 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     3241 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     3301 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     3361 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     3421 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     3481 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3541 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3601 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3661 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     3721 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     3781 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     3841 ttgcatttaa atttccgaac tctccaaggc cctcgtcgga aaatcttcaa acctttcgtc
     3901 cgatccatct tgcaggctac ctctcgaacg aactatcgca agtctcttgg ccggccttgc
     3961 gccttggcta ttgcttggca gcgcctatcg ccaggtatta ctccaatccc gaatatccga
     4021 gatcgggatc acccgagaga agttcaacct acatcctcaa tcccgatcta tccgagatcc
     4081 gaggaatatc gaaatcgggg cgcgcctggt gtaccgagaa cgatcctctc agtgcgagtc
     4141 tcgacgatcc atatcgttgc ttggcagtca gccagtcgga atccagcttg ggacccagga
     4201 agtccaatcg tcagatattg tactcaagcc tggtcacggc agcgtaccga tctgtttaaa
     4261 cctagatatt gatagtctga tcggtcaacg tataatcgag tcctagcttt tgcaaacatc
     4321 tatcaagaga caggatcagc aggaggcttt cgcatgattg aacaagatgg attgcacgca
     4381 ggttctccgg cggcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     4441 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgtccggt tctttttgtc
     4501 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     4561 ctggcgacga cgggcgttcc ttgcgcggct gtgctcgacg ttgtcactga agcgggaagg
     4621 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     4681 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     4741 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     4801 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     4861 ctgttcgcca ggctcaaggc gtctatgccc gacggcgagg atctcgtcgt gacccacggc
     4921 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     4981 ggccgtctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     5041 gaagagcttg gcggcgaatg ggctgaccgc ttccttgtgc tttacggtat cgccgcgccc
     5101 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgacc gattctaggt
     5161 gcattggcgc agaaaaaaat gcctgatgcg acgctgcgcg tcttatactc ccacatatgc
     5221 cagattcagc aacggatacg gcttccccaa cttgcccact tccatacgtg tcctccttac
     5281 cagaaattta tccttaaggt cgtttaaact cgactctggc tctatcgaat ctccgtcgtt
     5341 tcgagcttac gcgaacagcc gtggcgctca tttgctcgtc gggcatcgaa tctcgtcagc
     5401 tatcgtcagc ttaccttttt ggca
//
