LOCUS       pSF-MinProm-RLuc        4789 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector encoding a minimal promoter and Renilla luciferase, for 
            measuring the activity of transcriptional regulatory elements.
ACCESSION   .
VERSION     .
KEYWORDS    pSF-MinProm-RLuc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Oxford Genetics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     No poly(A) signal is present upstream of the MCS, so some background
            luciferase transcription may be observed.
FEATURES             Location/Qualifiers
     source          1..4789
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..89
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     terminator      complement(94..141)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    complement(158..206)
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    245..336
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     misc_feature    337..374
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        379..441
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     RBS             533..538
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             547..1482
                     /label=RLuc
                     /note="Renilla luciferase"
     misc_feature    1505..1515
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     misc_feature    1553..1563
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    complement(1595..1716)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     terminator      1830..1877
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      1907..2043
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    2088..2159
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     rep_origin      complement(2385..2973)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     RBS             3706..3711
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             3719..4510
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      4526..4662
                     /note="rrnG terminator"
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 gcgatcgcgg ctcccgacat cttggaccat tagctccaca ggtatcttct tccctctagt
       61 ggtcataaca gcagcttcag ctacctctca attcaaaaaa cccctcaaga cccgtttaga
      121 ggccccaagg ggttatgcta tcaatcgttg cgttacacac acaaaaaacc aacacacatc
      181 catcttcgat ggatagcgat tttattatct aactgctgat cgagtgtagc cagatctaag
      241 tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg
      301 tccccagtgc aaatgcaggt gccagaacat ttctctgtcg accaactagt accccgggta
      361 gggcccaatt cgaagtactt cgcatattaa ggtgacgcgt gtggcctcga acaccgagcg
      421 accctgcagc gacccgctta aaagatccag ccaccagatc tttgtcgatc ctaccatcca
      481 ctcgacacac ccgccagcgg ccgctgccaa gcttccgagc tctcgaattc aaaggaggta
      541 cccaccatga ccagcaaagt gtatgatccg gaacagcgca aacgcatgat taccggcccg
      601 cagtggtggg cgcgctgcaa acagatgaac gtgctggata gctttattaa ctattatgat
      661 agcgaaaaac atgcggaaaa cgcggtgatt tttctgcatg gcaacgcggc gagcagctat
      721 ctgtggcggc atgtggtgcc gcatattgaa ccagtggcgc gctgcattat tccggatctg
      781 attggcatgg gcaaaagcgg caaaagcggc aacggcagct atcgcctgct ggatcattat
      841 aaatatctga ccgcgtggtt tgaactgctg aacctgccga aaaaaattat ttttgtgggc
      901 catgattggg gcgcgtgcct ggcgtttcat tatagctatg aacatcagga taaaattaaa
      961 gcgattgtgc acgcggaaag cgtggtggat gtgattgaaa gctgggatga atggccggat
     1021 attgaagaag atattgcgct gattaaaagc gaagaaggcg aaaaaatggt gctggaaaac
     1081 aacttttttg tggaaaccat gctgccgagc aaaattatgc gcaaactgga accggaagaa
     1141 tttgcggcgt atctggaacc gtttaaagaa aaaggcgaag tgcgccgccc gaccctgagc
     1201 tggccgcgcg aaattccgct ggtgaaaggc ggcaaaccgg atgtggtcca gattgtgcgc
     1261 aactataacg cgtatctgcg cgcgagcgat gatctgccga aaatgtttat tgaaagcgat
     1321 ccgggctttt ttagcaacgc gattgtggaa ggcgcgaaaa aatttccgaa caccgaattt
     1381 gtgaaagtga agggcctgca ttttagccag gaagatgcgc cggatgaaat gggcaaatat
     1441 attaagagct ttgtggaacg cgtgctgaaa aacgaacagg gctagaagtt gtctcctcct
     1501 gcactgactg actgatacaa tcgatttctg gatccgcagg cctctgctag cttgactgac
     1561 tgagatacag cgtaccttca gctcacagac atgataagat acattgatga gtttggacaa
     1621 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     1681 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     1741 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     1801 tgtggtattg gcccatctct atcggtatcg tagcataacc ccttggggcc tctaaacggg
     1861 tcttgagggg ttttttgtgc ccctcgggcc ggattgctat ctaccggcat tggcgcagaa
     1921 aaaaatgcct gatgcgacgc tgcgcgtctt atactcccac atatgccaga ttcagcaacg
     1981 gatacggctt ccccaacttg cccacttcca tacgtgtcct ccttaccaga aatttatcct
     2041 taaggtcgtc agctatcctg caggcgatct ctcgatttcg atcaagacat tcctttaatg
     2101 gtcttttctg gacaccacta ggggtcagaa gtagttcatc aaactttctt ccctccctaa
     2161 tctcattggt taccttgggc tatcgaaact taattaacca gtcaagtcag ctacttggcg
     2221 agatcgactt gtctgggttt cgactacgct cagaattgcg tcagtcaagt tcgatctggt
     2281 ccttgctatt gcacccgttc tccgattacg agtttcattt aaatcatgtg agcaaaaggc
     2341 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     2401 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     2461 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     2521 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     2581 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     2641 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     2701 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     2761 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     2821 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     2881 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     2941 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     3001 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3061 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     3121 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     3181 atctgtctat ttcgttcatc catagttgca tttaaatttc cgaactctcc aaggccctcg
     3241 tcggaaaatc ttcaaacctt tcgtccgatc catcttgcag gctacctctc gaacgaacta
     3301 tcgcaagtct cttggccggc cttgcgcctt ggctattgct tggcagcgcc tatcgccagg
     3361 tattactcca atcccgaata tccgagatcg ggatcacccg agagaagttc aacctacatc
     3421 ctcaatcccg atctatccga gatccgagga atatcgaaat cggggcgcgc ctggtgtacc
     3481 gagaacgatc ctctcagtgc gagtctcgac gatccatatc gttgcttggc agtcagccag
     3541 tcggaatcca gcttgggacc caggaagtcc aatcgtcaga tattgtactc aagcctggtc
     3601 acggcagcgt accgatctgt ttaaacctag atattgatag tctgatcggt caacgtataa
     3661 tcgagtccta gcttttgcaa acatctatca agagacagga tcagcaggag gctttcgcat
     3721 gattgaacaa gatggattgc acgcaggttc tccggcggct tgggtggaga ggctattcgg
     3781 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     3841 gcaggggcgt ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     3901 agacgaggca gcgcggctat cgtggctggc gacgacgggc gttccttgcg cggctgtgct
     3961 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     4021 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     4081 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     4141 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     4201 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgtcta tgcccgacgg
     4261 cgaggatctc gtcgtgaccc acggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     4321 ccgcttttct ggattcatcg actgtggccg tctgggtgtg gcggaccgct atcaggacat
     4381 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     4441 tgtgctttac ggtatcgccg cgcccgattc gcagcgcatc gccttctatc gccttcttga
     4501 cgagttcttc tgaccgattc taggtgcatt ggcgcagaaa aaaatgcctg atgcgacgct
     4561 gcgcgtctta tactcccaca tatgccagat tcagcaacgg atacggcttc cccaacttgc
     4621 ccacttccat acgtgtcctc cttaccagaa atttatcctt aaggtcgttt aaactcgact
     4681 ctggctctat cgaatctccg tcgtttcgag cttacgcgaa cagccgtggc gctcatttgc
     4741 tcgtcgggca tcgaatctcg tcagctatcg tcagcttacc tttttggca
//
