LOCUS       pSF-MinProm-FLuc        5503 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector encoding a minimal promoter and firefly luciferase, for 
            measuring the activity of transcriptional regulatory elements.
ACCESSION   .
VERSION     .
KEYWORDS    pSF-MinProm-FLuc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Oxford Genetics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5503)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     No poly(A) signal is present upstream of the MCS, so some background
            luciferase transcription may be observed.
FEATURES             Location/Qualifiers
     source          1..5503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..89
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     terminator      complement(94..141)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    complement(158..206)
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    245..336
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     misc_feature    337..374
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        379..441
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     RBS             533..538
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             547..2196
                     /label=luciferase
                     /note="firefly luciferase"
     misc_feature    2219..2229
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     misc_feature    2267..2277
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    complement(2309..2430)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     terminator      2544..2591
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      2621..2757
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    2802..2873
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     rep_origin      complement(3099..3687)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     RBS             4420..4425
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             4433..5224
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      5240..5376
                     /note="rrnG terminator"
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 gcgatcgcgg ctcccgacat cttggaccat tagctccaca ggtatcttct tccctctagt
       61 ggtcataaca gcagcttcag ctacctctca attcaaaaaa cccctcaaga cccgtttaga
      121 ggccccaagg ggttatgcta tcaatcgttg cgttacacac acaaaaaacc aacacacatc
      181 catcttcgat ggatagcgat tttattatct aactgctgat cgagtgtagc cagatctaag
      241 tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg
      301 tccccagtgc aaatgcaggt gccagaacat ttctctgtcg accaactagt accccgggta
      361 gggcccaatt cgaagtactt cgcatattaa ggtgacgcgt gtggcctcga acaccgagcg
      421 accctgcagc gacccgctta aaagatccag ccaccagatc tttgtcgatc ctaccatcca
      481 ctcgacacac ccgccagcgg ccgctgccaa gcttccgagc tctcgaattc aaaggaggta
      541 cccaccatgg aagatgccaa aaacattaag aaaggcccag cgccattcta cccactcgaa
      601 gacgggaccg ctggcgagca gctgcataaa gccatgaagc gctacgccct ggtgcccggc
      661 accatcgcct ttaccgacgc acatatcgag gtggacatta cctacgccga gtacttcgag
      721 atgagcgttc ggctggcaga agctatgaag cgctatgggc tgaatacaaa ccatcggatc
      781 gtggtgtgta gcgagaatag cttgcagttc ttcatgcccg tgttgggtgc cctgttcatc
      841 ggtgtggctg tggccccagc taacgacatc tacaacgagc gcgagctgct gaacagcatg
      901 ggcatcagcc agcccaccgt cgtattcgtg agcaagaaag ggctgcaaaa gatcctcaac
      961 gtgcaaaaga agctaccgat catacaaaag atcatcatca tggatagcaa gaccgactac
     1021 cagggcttcc aaagcatgta caccttcgtg acttcccatt tgccacccgg cttcaacgag
     1081 tacgacttcg tgcccgagag cttcgaccgg gacaaaacca tcgccctgat catgaacagt
     1141 agtggcagta ccggattgcc caagggcgta gccctaccgc accgcaccgc ttgtgtccga
     1201 ttcagtcatg cccgcgaccc catcttcggc aaccagatca tccccgacac cgctatcctg
     1261 agcgtggtgc catttcacca cggcttcggc atgttcacca cgctgggcta cttgatctgc
     1321 ggctttcggg tcgtgctcat gtaccgcttc gaggaagagc tattcttgcg cagcttgcaa
     1381 gactataaga ttcaatctgc cctgctggtg cccacactat ttagcttctt cgctaagagc
     1441 actctcatcg acaagtacga cctaagcaac ttgcacgaga tcgccagcgg cggggcgccg
     1501 ctcagcaagg aggtaggtga ggccgtggcc aaacgcttcc acctaccagg catccgccag
     1561 ggctacggcc tgacagaaac aaccagcgcc attctgatca cccccgaagg ggacgacaag
     1621 cctggcgcag taggcaaggt ggtgcccttc ttcgaggcta aggtggtgga cttggacacc
     1681 ggtaagacac tgggtgtgaa ccagcgcggc gagctgtgcg tccgtggccc catgatcatg
     1741 agcggctacg ttaacaaccc cgaggctaca aacgctctca tcgacaagga cggctggctg
     1801 catagcggcg acatcgccta ctgggacgag gacgagcact tcttcatcgt ggaccggctg
     1861 aagagcctga tcaaatacaa gggctaccag gtagccccag ccgaactgga gagcatcctg
     1921 ctgcaacacc ccaacatctt cgacgccggg gtcgccggcc tgcccgacga cgatgccggc
     1981 gagctgcccg ccgcagtcgt cgtgctggaa cacggtaaaa ccatgaccga gaaggagatc
     2041 gtggactatg tggccagcca ggttacaacc gccaagaagc tgcgcggtgg tgttgtgttc
     2101 gtggacgagg tgcctaaagg actgaccggc aagttggacg cccgcaagat ccgcgagatt
     2161 ctcattaagg ccaagaaggg cggcaagatc gccgtctaga agttgtctcc tcctgcactg
     2221 actgactgat acaatcgatt tctggatccg caggcctctg ctagcttgac tgactgagat
     2281 acagcgtacc ttcagctcac agacatgata agatacattg atgagtttgg acaaaccaca
     2341 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     2401 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     2461 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     2521 attggcccat ctctatcggt atcgtagcat aaccccttgg ggcctctaaa cgggtcttga
     2581 ggggtttttt gtgcccctcg ggccggattg ctatctaccg gcattggcgc agaaaaaaat
     2641 gcctgatgcg acgctgcgcg tcttatactc ccacatatgc cagattcagc aacggatacg
     2701 gcttccccaa cttgcccact tccatacgtg tcctccttac cagaaattta tccttaaggt
     2761 cgtcagctat cctgcaggcg atctctcgat ttcgatcaag acattccttt aatggtcttt
     2821 tctggacacc actaggggtc agaagtagtt catcaaactt tcttccctcc ctaatctcat
     2881 tggttacctt gggctatcga aacttaatta accagtcaag tcagctactt ggcgagatcg
     2941 acttgtctgg gtttcgacta cgctcagaat tgcgtcagtc aagttcgatc tggtccttgc
     3001 tattgcaccc gttctccgat tacgagtttc atttaaatca tgtgagcaaa aggccagcaa
     3061 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     3121 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     3181 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     3241 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     3301 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3361 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3421 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3481 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     3541 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3601 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3661 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     3721 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     3781 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     3841 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     3901 ctatttcgtt catccatagt tgcatttaaa tttccgaact ctccaaggcc ctcgtcggaa
     3961 aatcttcaaa cctttcgtcc gatccatctt gcaggctacc tctcgaacga actatcgcaa
     4021 gtctcttggc cggccttgcg ccttggctat tgcttggcag cgcctatcgc caggtattac
     4081 tccaatcccg aatatccgag atcgggatca cccgagagaa gttcaaccta catcctcaat
     4141 cccgatctat ccgagatccg aggaatatcg aaatcggggc gcgcctggtg taccgagaac
     4201 gatcctctca gtgcgagtct cgacgatcca tatcgttgct tggcagtcag ccagtcggaa
     4261 tccagcttgg gacccaggaa gtccaatcgt cagatattgt actcaagcct ggtcacggca
     4321 gcgtaccgat ctgtttaaac ctagatattg atagtctgat cggtcaacgt ataatcgagt
     4381 cctagctttt gcaaacatct atcaagagac aggatcagca ggaggctttc gcatgattga
     4441 acaagatgga ttgcacgcag gttctccggc ggcttgggtg gagaggctat tcggctatga
     4501 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     4561 gcgtccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
     4621 ggcagcgcgg ctatcgtggc tggcgacgac gggcgttcct tgcgcggctg tgctcgacgt
     4681 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     4741 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     4801 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     4861 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     4921 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg tctatgcccg acggcgagga
     4981 tctcgtcgtg acccacggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     5041 ttctggattc atcgactgtg gccgtctggg tgtggcggac cgctatcagg acatagcgtt
     5101 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tccttgtgct
     5161 ttacggtatc gccgcgcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     5221 cttctgaccg attctaggtg cattggcgca gaaaaaaatg cctgatgcga cgctgcgcgt
     5281 cttatactcc cacatatgcc agattcagca acggatacgg cttccccaac ttgcccactt
     5341 ccatacgtgt cctccttacc agaaatttat ccttaaggtc gtttaaactc gactctggct
     5401 ctatcgaatc tccgtcgttt cgagcttacg cgaacagccg tggcgctcat ttgctcgtcg
     5461 ggcatcgaat ctcgtcagct atcgtcagct tacctttttg gca
//
