LOCUS       pSF-Core.        3734 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Promoterless backbone SnapFast(TM) cloning vector, on which all of 
            the other plasmids from Oxford Genetics are based.
ACCESSION   .
VERSION     .
KEYWORDS    pSF-Core
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Oxford Genetics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..89
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     terminator      complement(94..141)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    complement(158..206)
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    273..358
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     RBS             309..314
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     misc_signal     319..325
                     /label=Kozak sequence
                     /note="Kozak sequence"
     CDS             323..325
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     misc_feature    377..387
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     misc_feature    425..435
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    complement(467..588)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     terminator      702..749
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      779..915
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    960..1031
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     rep_origin      complement(1257..1845)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     RBS             2578..2583
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             2591..3448
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      3464..3600
                     /note="rrnG terminator"
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 gcgatcgcgg ctcccgacat cttggaccat tagctccaca ggtatcttct tccctctagt
       61 ggtcataaca gcagcttcag ctacctctca attcaaaaaa cccctcaaga cccgtttaga
      121 ggccccaagg ggttatgcta tcaatcgttg cgttacacac acaaaaaacc aacacacatc
      181 catcttcgat ggatagcgat tttattatct aactgctgat cgagtgtagc cagatctttg
      241 tcgatcctac catccactcg acacacccgc cagcggccgc tgccaagctt ccgagctctc
      301 gaattcaaag gaggtaccca ccatggggta ccgcgatatc tacctcgagg tttctagaag
      361 ttgtctcctc ctgcactgac tgactgatac aatcgatttc tggatccgca ggcctctgct
      421 agcttgactg actgagatac agcgtacctt cagctcacag acatgataag atacattgat
      481 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
      541 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
      601 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
      661 aacctctaca aatgtggtat tggcccatct ctatcggtat cgtagcataa ccccttgggg
      721 cctctaaacg ggtcttgagg ggttttttgt gcccctcggg ccggattgct atctaccggc
      781 attggcgcag aaaaaaatgc ctgatgcgac gctgcgcgtc ttatactccc acatatgcca
      841 gattcagcaa cggatacggc ttccccaact tgcccacttc catacgtgtc ctccttacca
      901 gaaatttatc cttaaggtcg tcagctatcc tgcaggcgat ctctcgattt cgatcaagac
      961 attcctttaa tggtcttttc tggacaccac taggggtcag aagtagttca tcaaactttc
     1021 ttccctccct aatctcattg gttaccttgg gctatcgaaa cttaattaac cagtcaagtc
     1081 agctacttgg cgagatcgac ttgtctgggt ttcgactacg ctcagaattg cgtcagtcaa
     1141 gttcgatctg gtccttgcta ttgcacccgt tctccgatta cgagtttcat ttaaatcatg
     1201 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     1261 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     1321 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     1381 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     1441 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     1501 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1561 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     1621 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     1681 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     1741 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     1801 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1861 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1921 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     1981 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     2041 cctatctcag cgatctgtct atttcgttca tccatagttg catttaaatt tccgaactct
     2101 ccaaggccct cgtcggaaaa tcttcaaacc tttcgtccga tccatcttgc aggctacctc
     2161 tcgaacgaac tatcgcaagt ctcttggccg gccttgcgcc ttggctattg cttggcagcg
     2221 cctatcgcca ggtattactc caatcccgaa tatccgagat cgggatcacc cgagagaagt
     2281 tcaacctaca tcctcaatcc cgatctatcc gagatccgag gaatatcgaa atcggggcgc
     2341 gcctggtgta ccgagaacga tcctctcagt gcgagtctcg acgatccata tcgttgcttg
     2401 gcagtcagcc agtcggaatc cagcttggga cccaggaagt ccaatcgtca gatattgtac
     2461 tcaagcctgg tcacggcagc gtaccgatct gtttaaacct agatattgat agtctgatcg
     2521 gtcaacgtat aatcgagtcc tagcttttgc aaacatctat caagagacag gatcagcagg
     2581 aggctttcgc atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     2641 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     2701 gttgggtgcg cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     2761 ttttcgcccc gaagaacgct ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     2821 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     2881 gaatgacttg gttgagtatt caccagtcac agaaaagcat cttacggatg gcatgacagt
     2941 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     3001 gacaacgatt ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     3061 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     3121 caccacgatg cctgtagcaa tggcaacaac cttgcgtaaa ctattaactg gcgaactact
     3181 tactctagct tcccggcaac agttgataga ctggatggag gcggataaag ttgcaggacc
     3241 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     3301 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     3361 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     3421 gataggtgcc tcactgatta agcattggta accgattcta ggtgcattgg cgcagaaaaa
     3481 aatgcctgat gcgacgctgc gcgtcttata ctcccacata tgccagattc agcaacggat
     3541 acggcttccc caacttgccc acttccatac gtgtcctcct taccagaaat ttatccttaa
     3601 gatcccgaat cgtttaaact cgactctggc tctatcgaat ctccgtcgtt tcgagcttac
     3661 gcgaacagcc gtggcgctca tttgctcgtc gggcatcgaa tctcgtcagc tatcgtcagc
     3721 ttaccttttt ggca
//
