LOCUS       pSF-CMVe-RLuc.        5198 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector encoding the CMV enhancer and Renilla luciferase, for 
            measuring the activity of promoters.
ACCESSION   .
VERSION     .
KEYWORDS    pSF-CMVe-RLuc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Oxford Genetics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     No poly(A) signal is present between the CMV enhancer and the MCS, 
            so some background luciferase transcription may be observed.
FEATURES             Location/Qualifiers
     source          1..5198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..89
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     terminator      complement(94..141)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    complement(158..206)
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     enhancer        298..601
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    732..823
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     misc_feature    824..861
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     RBS             942..947
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             956..1891
                     /label=RLuc
                     /note="Renilla luciferase"
     misc_feature    1914..1924
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     misc_feature    1962..1972
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    complement(2004..2125)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     terminator      2239..2286
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      2316..2452
                     /note="rrnG terminator"
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    2497..2568
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     rep_origin      complement(2794..3382)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     RBS             4115..4120
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             4128..4919
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      4935..5071
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 gcgatcgcgg ctcccgacat cttggaccat tagctccaca ggtatcttct tccctctagt
       61 ggtcataaca gcagcttcag ctacctctca attcaaaaaa cccctcaaga cccgtttaga
      121 ggccccaagg ggttatgcta tcaatcgttg cgttacacac acaaaaaacc aacacacatc
      181 catcttcgat ggatagcgat tttattatct aactgctgat cgagtgtagc cagatcttag
      241 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      301 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      361 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      421 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      481 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      541 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      601 gctgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      661 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggctcgtcg cgacctttgt
      721 cgagaagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa
      781 taggctgtcc ccagtgcaaa tgcaggtgcc agaacatttc tctgtcgacc aactagtacc
      841 ccgggtaggg cccaattcga aaccagatct ttgtcgatcc taccatccac tcgacacacc
      901 cgccagcggc cgctgccaag cttccgagct ctcgaattca aaggaggtac ccaccatgac
      961 cagcaaagtg tatgatccgg aacagcgcaa acgcatgatt accggcccgc agtggtgggc
     1021 gcgctgcaaa cagatgaacg tgctggatag ctttattaac tattatgata gcgaaaaaca
     1081 tgcggaaaac gcggtgattt ttctgcatgg caacgcggcg agcagctatc tgtggcggca
     1141 tgtggtgccg catattgaac cagtggcgcg ctgcattatt ccggatctga ttggcatggg
     1201 caaaagcggc aaaagcggca acggcagcta tcgcctgctg gatcattata aatatctgac
     1261 cgcgtggttt gaactgctga acctgccgaa aaaaattatt tttgtgggcc atgattgggg
     1321 cgcgtgcctg gcgtttcatt atagctatga acatcaggat aaaattaaag cgattgtgca
     1381 cgcggaaagc gtggtggatg tgattgaaag ctgggatgaa tggccggata ttgaagaaga
     1441 tattgcgctg attaaaagcg aagaaggcga aaaaatggtg ctggaaaaca acttttttgt
     1501 ggaaaccatg ctgccgagca aaattatgcg caaactggaa ccggaagaat ttgcggcgta
     1561 tctggaaccg tttaaagaaa aaggcgaagt gcgccgcccg accctgagct ggccgcgcga
     1621 aattccgctg gtgaaaggcg gcaaaccgga tgtggtccag attgtgcgca actataacgc
     1681 gtatctgcgc gcgagcgatg atctgccgaa aatgtttatt gaaagcgatc cgggcttttt
     1741 tagcaacgcg attgtggaag gcgcgaaaaa atttccgaac accgaatttg tgaaagtgaa
     1801 gggcctgcat tttagccagg aagatgcgcc ggatgaaatg ggcaaatata ttaagagctt
     1861 tgtggaacgc gtgctgaaaa acgaacaggg ctagaagttg tctcctcctg cactgactga
     1921 ctgatacaat cgatttctgg atccgcaggc ctctgctagc ttgactgact gagatacagc
     1981 gtaccttcag ctcacagaca tgataagata cattgatgag tttggacaaa ccacaactag
     2041 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     2101 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     2161 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtattgg
     2221 cccatctcta tcggtatcgt agcataaccc cttggggcct ctaaacgggt cttgaggggt
     2281 tttttgtgcc cctcgggccg gattgctatc taccggcatt ggcgcagaaa aaaatgcctg
     2341 atgcgacgct gcgcgtctta tactcccaca tatgccagat tcagcaacgg atacggcttc
     2401 cccaacttgc ccacttccat acgtgtcctc cttaccagaa atttatcctt aaggtcgtca
     2461 gctatcctgc aggcgatctc tcgatttcga tcaagacatt cctttaatgg tcttttctgg
     2521 acaccactag gggtcagaag tagttcatca aactttcttc cctccctaat ctcattggtt
     2581 accttgggct atcgaaactt aattaaccag tcaagtcagc tacttggcga gatcgacttg
     2641 tctgggtttc gactacgctc agaattgcgt cagtcaagtt cgatctggtc cttgctattg
     2701 cacccgttct ccgattacga gtttcattta aatcatgtga gcaaaaggcc agcaaaaggc
     2761 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2821 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2881 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2941 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     3001 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     3061 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     3121 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     3181 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     3241 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     3301 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     3361 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     3421 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     3481 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     3541 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     3601 tcgttcatcc atagttgcat ttaaatttcc gaactctcca aggccctcgt cggaaaatct
     3661 tcaaaccttt cgtccgatcc atcttgcagg ctacctctcg aacgaactat cgcaagtctc
     3721 ttggccggcc ttgcgccttg gctattgctt ggcagcgcct atcgccaggt attactccaa
     3781 tcccgaatat ccgagatcgg gatcacccga gagaagttca acctacatcc tcaatcccga
     3841 tctatccgag atccgaggaa tatcgaaatc ggggcgcgcc tggtgtaccg agaacgatcc
     3901 tctcagtgcg agtctcgacg atccatatcg ttgcttggca gtcagccagt cggaatccag
     3961 cttgggaccc aggaagtcca atcgtcagat attgtactca agcctggtca cggcagcgta
     4021 ccgatctgtt taaacctaga tattgatagt ctgatcggtc aacgtataat cgagtcctag
     4081 cttttgcaaa catctatcaa gagacaggat cagcaggagg ctttcgcatg attgaacaag
     4141 atggattgca cgcaggttct ccggcggctt gggtggagag gctattcggc tatgactggg
     4201 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgtc
     4261 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
     4321 cgcggctatc gtggctggcg acgacgggcg ttccttgcgc ggctgtgctc gacgttgtca
     4381 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     4441 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     4501 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     4561 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     4621 tcgcgccagc cgaactgttc gccaggctca aggcgtctat gcccgacggc gaggatctcg
     4681 tcgtgaccca cggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     4741 gattcatcga ctgtggccgt ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     4801 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctt gtgctttacg
     4861 gtatcgccgc gcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     4921 gaccgattct aggtgcattg gcgcagaaaa aaatgcctga tgcgacgctg cgcgtcttat
     4981 actcccacat atgccagatt cagcaacgga tacggcttcc ccaacttgcc cacttccata
     5041 cgtgtcctcc ttaccagaaa tttatcctta aggtcgttta aactcgactc tggctctatc
     5101 gaatctccgt cgtttcgagc ttacgcgaac agccgtggcg ctcatttgct cgtcgggcat
     5161 cgaatctcgt cagctatcgt cagcttacct ttttggca
//
