LOCUS       pSF-CMVe-FLuc.        5912 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector encoding the CMV enhancer and firefly luciferase, for 
            measuring the activity of promoters.
ACCESSION   .
VERSION     .
KEYWORDS    pSF-CMVe-FLuc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Oxford Genetics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5912)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     No poly(A) signal is present between the CMV enhancer and the MCS, 
            so some background luciferase transcription may be observed.
FEATURES             Location/Qualifiers
     source          1..5912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..89
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     terminator      complement(94..141)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    complement(158..206)
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     enhancer        298..601
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    732..823
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     misc_feature    824..861
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     RBS             942..947
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             956..2605
                     /label=luciferase
                     /note="firefly luciferase"
     misc_feature    2628..2638
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     misc_feature    2676..2686
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    complement(2718..2839)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     terminator      2953..3000
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      3030..3166
                     /note="rrnG terminator"
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    3211..3282
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     rep_origin      complement(3508..4096)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     RBS             4829..4834
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             4842..5633
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      5649..5785
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 gcgatcgcgg ctcccgacat cttggaccat tagctccaca ggtatcttct tccctctagt
       61 ggtcataaca gcagcttcag ctacctctca attcaaaaaa cccctcaaga cccgtttaga
      121 ggccccaagg ggttatgcta tcaatcgttg cgttacacac acaaaaaacc aacacacatc
      181 catcttcgat ggatagcgat tttattatct aactgctgat cgagtgtagc cagatcttag
      241 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      301 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      361 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      421 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      481 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      541 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      601 gctgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      661 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggctcgtcg cgacctttgt
      721 cgagaagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa
      781 taggctgtcc ccagtgcaaa tgcaggtgcc agaacatttc tctgtcgacc aactagtacc
      841 ccgggtaggg cccaattcga aaccagatct ttgtcgatcc taccatccac tcgacacacc
      901 cgccagcggc cgctgccaag cttccgagct ctcgaattca aaggaggtac ccaccatgga
      961 agatgccaaa aacattaaga aaggcccagc gccattctac ccactcgaag acgggaccgc
     1021 tggcgagcag ctgcataaag ccatgaagcg ctacgccctg gtgcccggca ccatcgcctt
     1081 taccgacgca catatcgagg tggacattac ctacgccgag tacttcgaga tgagcgttcg
     1141 gctggcagaa gctatgaagc gctatgggct gaatacaaac catcggatcg tggtgtgtag
     1201 cgagaatagc ttgcagttct tcatgcccgt gttgggtgcc ctgttcatcg gtgtggctgt
     1261 ggccccagct aacgacatct acaacgagcg cgagctgctg aacagcatgg gcatcagcca
     1321 gcccaccgtc gtattcgtga gcaagaaagg gctgcaaaag atcctcaacg tgcaaaagaa
     1381 gctaccgatc atacaaaaga tcatcatcat ggatagcaag accgactacc agggcttcca
     1441 aagcatgtac accttcgtga cttcccattt gccacccggc ttcaacgagt acgacttcgt
     1501 gcccgagagc ttcgaccggg acaaaaccat cgccctgatc atgaacagta gtggcagtac
     1561 cggattgccc aagggcgtag ccctaccgca ccgcaccgct tgtgtccgat tcagtcatgc
     1621 ccgcgacccc atcttcggca accagatcat ccccgacacc gctatcctga gcgtggtgcc
     1681 atttcaccac ggcttcggca tgttcaccac gctgggctac ttgatctgcg gctttcgggt
     1741 cgtgctcatg taccgcttcg aggaagagct attcttgcgc agcttgcaag actataagat
     1801 tcaatctgcc ctgctggtgc ccacactatt tagcttcttc gctaagagca ctctcatcga
     1861 caagtacgac ctaagcaact tgcacgagat cgccagcggc ggggcgccgc tcagcaagga
     1921 ggtaggtgag gccgtggcca aacgcttcca cctaccaggc atccgccagg gctacggcct
     1981 gacagaaaca accagcgcca ttctgatcac ccccgaaggg gacgacaagc ctggcgcagt
     2041 aggcaaggtg gtgcccttct tcgaggctaa ggtggtggac ttggacaccg gtaagacact
     2101 gggtgtgaac cagcgcggcg agctgtgcgt ccgtggcccc atgatcatga gcggctacgt
     2161 taacaacccc gaggctacaa acgctctcat cgacaaggac ggctggctgc atagcggcga
     2221 catcgcctac tgggacgagg acgagcactt cttcatcgtg gaccggctga agagcctgat
     2281 caaatacaag ggctaccagg tagccccagc cgaactggag agcatcctgc tgcaacaccc
     2341 caacatcttc gacgccgggg tcgccggcct gcccgacgac gatgccggcg agctgcccgc
     2401 cgcagtcgtc gtgctggaac acggtaaaac catgaccgag aaggagatcg tggactatgt
     2461 ggccagccag gttacaaccg ccaagaagct gcgcggtggt gttgtgttcg tggacgaggt
     2521 gcctaaagga ctgaccggca agttggacgc ccgcaagatc cgcgagattc tcattaaggc
     2581 caagaagggc ggcaagatcg ccgtctagaa gttgtctcct cctgcactga ctgactgata
     2641 caatcgattt ctggatccgc aggcctctgc tagcttgact gactgagata cagcgtacct
     2701 tcagctcaca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     2761 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     2821 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     2881 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta ttggcccatc
     2941 tctatcggta tcgtagcata accccttggg gcctctaaac gggtcttgag gggttttttg
     3001 tgcccctcgg gccggattgc tatctaccgg cattggcgca gaaaaaaatg cctgatgcga
     3061 cgctgcgcgt cttatactcc cacatatgcc agattcagca acggatacgg cttccccaac
     3121 ttgcccactt ccatacgtgt cctccttacc agaaatttat ccttaaggtc gtcagctatc
     3181 ctgcaggcga tctctcgatt tcgatcaaga cattccttta atggtctttt ctggacacca
     3241 ctaggggtca gaagtagttc atcaaacttt cttccctccc taatctcatt ggttaccttg
     3301 ggctatcgaa acttaattaa ccagtcaagt cagctacttg gcgagatcga cttgtctggg
     3361 tttcgactac gctcagaatt gcgtcagtca agttcgatct ggtccttgct attgcacccg
     3421 ttctccgatt acgagtttca tttaaatcat gtgagcaaaa ggccagcaaa aggccaggaa
     3481 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     3541 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     3601 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     3661 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     3721 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     3781 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     3841 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     3901 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     3961 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     4021 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     4081 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     4141 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     4201 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     4261 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     4321 atccatagtt gcatttaaat ttccgaactc tccaaggccc tcgtcggaaa atcttcaaac
     4381 ctttcgtccg atccatcttg caggctacct ctcgaacgaa ctatcgcaag tctcttggcc
     4441 ggccttgcgc cttggctatt gcttggcagc gcctatcgcc aggtattact ccaatcccga
     4501 atatccgaga tcgggatcac ccgagagaag ttcaacctac atcctcaatc ccgatctatc
     4561 cgagatccga ggaatatcga aatcggggcg cgcctggtgt accgagaacg atcctctcag
     4621 tgcgagtctc gacgatccat atcgttgctt ggcagtcagc cagtcggaat ccagcttggg
     4681 acccaggaag tccaatcgtc agatattgta ctcaagcctg gtcacggcag cgtaccgatc
     4741 tgtttaaacc tagatattga tagtctgatc ggtcaacgta taatcgagtc ctagcttttg
     4801 caaacatcta tcaagagaca ggatcagcag gaggctttcg catgattgaa caagatggat
     4861 tgcacgcagg ttctccggcg gcttgggtgg agaggctatt cggctatgac tgggcacaac
     4921 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgtccggttc
     4981 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
     5041 tatcgtggct ggcgacgacg ggcgttcctt gcgcggctgt gctcgacgtt gtcactgaag
     5101 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     5161 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     5221 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     5281 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     5341 cagccgaact gttcgccagg ctcaaggcgt ctatgcccga cggcgaggat ctcgtcgtga
     5401 cccacggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     5461 tcgactgtgg ccgtctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     5521 atattgctga agagcttggc ggcgaatggg ctgaccgctt ccttgtgctt tacggtatcg
     5581 ccgcgcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgaccga
     5641 ttctaggtgc attggcgcag aaaaaaatgc ctgatgcgac gctgcgcgtc ttatactccc
     5701 acatatgcca gattcagcaa cggatacggc ttccccaact tgcccacttc catacgtgtc
     5761 ctccttacca gaaatttatc cttaaggtcg tttaaactcg actctggctc tatcgaatct
     5821 ccgtcgtttc gagcttacgc gaacagccgt ggcgctcatt tgctcgtcgg gcatcgaatc
     5881 tcgtcagcta tcgtcagctt acctttttgg ca
//
