LOCUS       pSF-CMV-Ub-RLuc        6486 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector for co-expressing a gene together with Renilla 
            luciferase expressed from the ubiquitin C promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pSF-CMV-Ub-RLuc AscI
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6486)
  AUTHORS   Oxford Genetics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6486)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..89
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     terminator      complement(94..141)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    complement(158..206)
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     enhancer        287..590
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        591..794
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    857..942
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     RBS             893..898
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     misc_signal     903..909
                     /label=Kozak sequence
                     /note="Kozak sequence"
     CDS             907..909
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     misc_feature    961..971
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     misc_feature    1009..1019
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    complement(1051..1172)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     terminator      1286..1333
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      1363..1499
                     /note="rrnG terminator"
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    1544..1615
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     rep_origin      complement(1841..2429)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2929..4138
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     CDS             4142..5077
                     /label=RLuc
                     /note="Renilla luciferase"
     polyA_signal    5102..5150
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     RBS             5403..5408
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             5416..6207
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      6223..6359
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 gcgatcgcgg ctcccgacat cttggaccat tagctccaca ggtatcttct tccctctagt
       61 ggtcataaca gcagcttcag ctacctctca attcaaaaaa cccctcaaga cccgtttaga
      121 ggccccaagg ggttatgcta tcaatcgttg cgttacacac acaaaaaacc aacacacatc
      181 catcttcgat ggatagcgat tttattatct aactgctgat cgagtgtagc cagatctagt
      241 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      301 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      361 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      421 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      481 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      541 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg ctgatgcggt
      601 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      661 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      721 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      781 atataagcag agctggttta gtgaaccgtc agatcagatc tttgtcgatc ctaccatcca
      841 ctcgacacac ccgccagcgg ccgctgccaa gcttccgagc tctcgaattc aaaggaggta
      901 cccaccatgg ggtaccgcga tatctacctc gaggtttcta gaagttgtct cctcctgcac
      961 tgactgactg atacaatcga tttctggatc cgcaggcctc tgctagcttg actgactgag
     1021 atacagcgta ccttcagctc acagacatga taagatacat tgatgagttt ggacaaacca
     1081 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     1141 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     1201 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     1261 gtattggccc atctctatcg gtatcgtagc ataacccctt ggggcctcta aacgggtctt
     1321 gaggggtttt ttgtgcccct cgggccggat tgctatctac cggcattggc gcagaaaaaa
     1381 atgcctgatg cgacgctgcg cgtcttatac tcccacatat gccagattca gcaacggata
     1441 cggcttcccc aacttgccca cttccatacg tgtcctcctt accagaaatt tatccttaag
     1501 gtcgtcagct atcctgcagg cgatctctcg atttcgatca agacattcct ttaatggtct
     1561 tttctggaca ccactagggg tcagaagtag ttcatcaaac tttcttccct ccctaatctc
     1621 attggttacc ttgggctatc gaaacttaat taaccagtca agtcagctac ttggcgagat
     1681 cgacttgtct gggtttcgac tacgctcaga attgcgtcag tcaagttcga tctggtcctt
     1741 gctattgcac ccgttctccg attacgagtt tcatttaaat catgtgagca aaaggccagc
     1801 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     1861 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     1921 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     1981 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2041 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2101 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2161 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     2221 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2281 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     2341 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2401 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     2461 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     2521 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     2581 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     2641 gtctatttcg ttcatccata gttgcattta aatttccgaa ctctccaagg ccctcgtcgg
     2701 aaaatcttca aacctttcgt ccgatccatc ttgcaggcta cctctcgaac gaactatcgc
     2761 aagtctcttg gccggccttg cgccttggct attgcttggc agcgcctatc gccaggtatt
     2821 actccaatcc cgaatatccg agatcgggat cacccgagag aagttcaacc tacatcctca
     2881 atcccgatct atccgagatc cgaggaatat cgaaatcggg gcgcgcctgg cctccgcgcc
     2941 gggttttggc gcctcccgcg ggcgcccccc tcgtcacggc gagcgctgcc acgtcagacg
     3001 aagggcgcag gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc
     3061 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg
     3121 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct
     3181 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga
     3241 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt
     3301 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc
     3361 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt
     3421 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg
     3481 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca
     3541 aggtgggggg catggtgggc ggcaagaacc caaggtcttg agcccttcgc taatgcggga
     3601 aagctcttat tcgggtgaga tgggctgggc accatctggg gaccctgacg tgaagtttgt
     3661 cactgactgg agaactcggt ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt
     3721 tgggcagtgc acccgtacct ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt
     3781 tctgttggct tataatgcag ggtggggcca cctgccggta ggtgtgcggt aggcttttct
     3841 ccgtcgcagg acgcagggtt cgggcctagg gtaggctctc ctgaatcgac aggcgccgga
     3901 cctctggtga ggggagggat aagtgaggcg tcagtttctt tggtcggttt tatgtaccta
     3961 tcttcttaag tagctgaagc tccggttttg aactatgcgc tcggggttgg cgagtgtgtt
     4021 ttgtgaagtt ttttaggcac cttttgaaat gtaatcattt gggtcaatat gtaattttca
     4081 gtgttagact tgtaaattgt ccgctaaatt ctggccgttt ttggcttttt tgttagacaa
     4141 catgaccagc aaagtgtatg atccggaaca gcgcaaacgc atgattaccg gcccgcagtg
     4201 gtgggcgcgc tgcaaacaga tgaacgtgct ggatagcttt attaactatt atgatagcga
     4261 aaaacatgcg gaaaacgcgg tgatttttct gcatggcaac gcggcgagca gctatctgtg
     4321 gcggcatgtg gtgccgcata ttgaaccagt ggcgcgctgc attattccgg atctgattgg
     4381 catgggcaaa agcggcaaaa gcggcaacgg cagctatcgc ctgctggatc attataaata
     4441 tctgaccgcg tggtttgaac tgctgaacct gccgaaaaaa attatttttg tgggccatga
     4501 ttggggcgcg tgcctggcgt ttcattatag ctatgaacat caggataaaa ttaaagcgat
     4561 tgtgcacgcg gaaagcgtgg tggatgtgat tgaaagctgg gatgaatggc cggatattga
     4621 agaagatatt gcgctgatta aaagcgaaga aggcgaaaaa atggtgctgg aaaacaactt
     4681 ttttgtggaa accatgctgc cgagcaaaat tatgcgcaaa ctggaaccgg aagaatttgc
     4741 ggcgtatctg gaaccgttta aagaaaaagg cgaagtgcgc cgcccgaccc tgagctggcc
     4801 gcgcgaaatt ccgctggtga aaggcggcaa accggatgtg gtccagattg tgcgcaacta
     4861 taacgcgtat ctgcgcgcga gcgatgatct gccgaaaatg tttattgaaa gcgatccggg
     4921 cttttttagc aacgcgattg tggaaggcgc gaaaaaattt ccgaacaccg aatttgtgaa
     4981 agtgaagggc ctgcatttta gccaggaaga tgcgccggat gaaatgggca aatatattaa
     5041 gagctttgtg gaacgcgtgc tgaaaaacga acagggctag tatgtaagcc tagtcttaga
     5101 taataaaatc gctatccatc gaagatggat gtgtgttggt tttttgtgtg tgtaacgcta
     5161 ggcgcgcctg gtgtaccgag aacgatcctc tcagtgcgag tctcgacgat ccatatcgtt
     5221 gcttggcagt cagccagtcg gaatccagct tgggacccag gaagtccaat cgtcagatat
     5281 tgtactcaag cctggtcacg gcagcgtacc gatctgttta aacctagata ttgatagtct
     5341 gatcggtcaa cgtataatcg agtcctagct tttgcaaaca tctatcaaga gacaggatca
     5401 gcaggaggct ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggcggcttgg
     5461 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     5521 gtgttccggc tgtcagcgca ggggcgtccg gttctttttg tcaagaccga cctgtccggt
     5581 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggcgac gacgggcgtt
     5641 ccttgcgcgg ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     5701 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     5761 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     5821 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     5881 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     5941 gcgtctatgc ccgacggcga ggatctcgtc gtgacccacg gcgatgcctg cttgccgaat
     6001 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccgtct gggtgtggcg
     6061 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     6121 tgggctgacc gcttccttgt gctttacggt atcgccgcgc ccgattcgca gcgcatcgcc
     6181 ttctatcgcc ttcttgacga gttcttctga ccgattctag gtgcattggc gcagaaaaaa
     6241 atgcctgatg cgacgctgcg cgtcttatac tcccacatat gccagattca gcaacggata
     6301 cggcttcccc aacttgccca cttccatacg tgtcctcctt accagaaatt tatccttaag
     6361 gtcgtttaaa ctcgactctg gctctatcga atctccgtcg tttcgagctt acgcgaacag
     6421 ccgtggcgct catttgctcg tcgggcatcg aatctcgtca gctatcgtca gcttaccttt
     6481 ttggca
//
