LOCUS       pSF-CMV-RSV-RLuc        5729 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector for co-expressing a gene together with Renilla 
            luciferase expressed from the Rous sarcoma virus promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pSF-CMV-RSV-RLuc AscI
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5729)
  AUTHORS   Oxford Genetics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5729)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..89
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     terminator      complement(94..141)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    complement(158..206)
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     enhancer        287..590
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        591..794
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    857..942
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     RBS             893..898
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     misc_signal     903..909
                     /label=Kozak sequence
                     /note="Kozak sequence"
     CDS             907..909
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     misc_feature    961..971
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     misc_feature    1009..1019
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    complement(1051..1172)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     terminator      1286..1333
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      1363..1499
                     /note="rrnG terminator"
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    1544..1615
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     rep_origin      complement(1841..2429)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3110..3372
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     CDS             3385..4320
                     /label=RLuc
                     /note="Renilla luciferase"
     polyA_signal    4345..4393
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     RBS             4646..4651
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             4659..5450
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      5466..5602
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 gcgatcgcgg ctcccgacat cttggaccat tagctccaca ggtatcttct tccctctagt
       61 ggtcataaca gcagcttcag ctacctctca attcaaaaaa cccctcaaga cccgtttaga
      121 ggccccaagg ggttatgcta tcaatcgttg cgttacacac acaaaaaacc aacacacatc
      181 catcttcgat ggatagcgat tttattatct aactgctgat cgagtgtagc cagatctagt
      241 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      301 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      361 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      421 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      481 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      541 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg ctgatgcggt
      601 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      661 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      721 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      781 atataagcag agctggttta gtgaaccgtc agatcagatc tttgtcgatc ctaccatcca
      841 ctcgacacac ccgccagcgg ccgctgccaa gcttccgagc tctcgaattc aaaggaggta
      901 cccaccatgg ggtaccgcga tatctacctc gaggtttcta gaagttgtct cctcctgcac
      961 tgactgactg atacaatcga tttctggatc cgcaggcctc tgctagcttg actgactgag
     1021 atacagcgta ccttcagctc acagacatga taagatacat tgatgagttt ggacaaacca
     1081 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     1141 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     1201 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     1261 gtattggccc atctctatcg gtatcgtagc ataacccctt ggggcctcta aacgggtctt
     1321 gaggggtttt ttgtgcccct cgggccggat tgctatctac cggcattggc gcagaaaaaa
     1381 atgcctgatg cgacgctgcg cgtcttatac tcccacatat gccagattca gcaacggata
     1441 cggcttcccc aacttgccca cttccatacg tgtcctcctt accagaaatt tatccttaag
     1501 gtcgtcagct atcctgcagg cgatctctcg atttcgatca agacattcct ttaatggtct
     1561 tttctggaca ccactagggg tcagaagtag ttcatcaaac tttcttccct ccctaatctc
     1621 attggttacc ttgggctatc gaaacttaat taaccagtca agtcagctac ttggcgagat
     1681 cgacttgtct gggtttcgac tacgctcaga attgcgtcag tcaagttcga tctggtcctt
     1741 gctattgcac ccgttctccg attacgagtt tcatttaaat catgtgagca aaaggccagc
     1801 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     1861 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     1921 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     1981 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2041 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2101 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2161 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     2221 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2281 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     2341 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2401 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     2461 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     2521 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     2581 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     2641 gtctatttcg ttcatccata gttgcattta aatttccgaa ctctccaagg ccctcgtcgg
     2701 aaaatcttca aacctttcgt ccgatccatc ttgcaggcta cctctcgaac gaactatcgc
     2761 aagtctcttg gccggccttg cgccttggct attgcttggc agcgcctatc gccaggtatt
     2821 actccaatcc cgaatatccg agatcgggat cacccgagag aagttcaacc tacatcctca
     2881 atcccgatct atccgagatc cgaggaatat cgaaatcggg gcgcgcccga caattgcatg
     2941 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
     3001 cgtatctgag gggactaggg tgtgtttagg cgaaaagcgg ggcttcggtt gtacgcggtt
     3061 aggagtcccc tcaggatata gtagtttcgc ttttgcatag ggagggggaa atgtagtctt
     3121 atgcaataca cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag
     3181 gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat
     3241 taggaaggca acagacaggt ctgacatgga ttggacgaac cactgaattg cgcattgcag
     3301 agataattgt atttaagtgc ctagctcgat acaataaacg ccatttgacc attcaccaca
     3361 ttggtgtgca cctccaaggc caacatgacc agcaaagtgt atgatccgga acagcgcaaa
     3421 cgcatgatta ccggcccgca gtggtgggcg cgctgcaaac agatgaacgt gctggatagc
     3481 tttattaact attatgatag cgaaaaacat gcggaaaacg cggtgatttt tctgcatggc
     3541 aacgcggcga gcagctatct gtggcggcat gtggtgccgc atattgaacc agtggcgcgc
     3601 tgcattattc cggatctgat tggcatgggc aaaagcggca aaagcggcaa cggcagctat
     3661 cgcctgctgg atcattataa atatctgacc gcgtggtttg aactgctgaa cctgccgaaa
     3721 aaaattattt ttgtgggcca tgattggggc gcgtgcctgg cgtttcatta tagctatgaa
     3781 catcaggata aaattaaagc gattgtgcac gcggaaagcg tggtggatgt gattgaaagc
     3841 tgggatgaat ggccggatat tgaagaagat attgcgctga ttaaaagcga agaaggcgaa
     3901 aaaatggtgc tggaaaacaa cttttttgtg gaaaccatgc tgccgagcaa aattatgcgc
     3961 aaactggaac cggaagaatt tgcggcgtat ctggaaccgt ttaaagaaaa aggcgaagtg
     4021 cgccgcccga ccctgagctg gccgcgcgaa attccgctgg tgaaaggcgg caaaccggat
     4081 gtggtccaga ttgtgcgcaa ctataacgcg tatctgcgcg cgagcgatga tctgccgaaa
     4141 atgtttattg aaagcgatcc gggctttttt agcaacgcga ttgtggaagg cgcgaaaaaa
     4201 tttccgaaca ccgaatttgt gaaagtgaag ggcctgcatt ttagccagga agatgcgccg
     4261 gatgaaatgg gcaaatatat taagagcttt gtggaacgcg tgctgaaaaa cgaacagggc
     4321 tagtatgtaa gcctagtctt agataataaa atcgctatcc atcgaagatg gatgtgtgtt
     4381 ggttttttgt gtgtgtaacg ctaggcgcgc ctggtgtacc gagaacgatc ctctcagtgc
     4441 gagtctcgac gatccatatc gttgcttggc agtcagccag tcggaatcca gcttgggacc
     4501 caggaagtcc aatcgtcaga tattgtactc aagcctggtc acggcagcgt accgatctgt
     4561 ttaaacctag atattgatag tctgatcggt caacgtataa tcgagtccta gcttttgcaa
     4621 acatctatca agagacagga tcagcaggag gctttcgcat gattgaacaa gatggattgc
     4681 acgcaggttc tccggcggct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     4741 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgt ccggttcttt
     4801 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
     4861 cgtggctggc gacgacgggc gttccttgcg cggctgtgct cgacgttgtc actgaagcgg
     4921 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     4981 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     5041 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     5101 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     5161 ccgaactgtt cgccaggctc aaggcgtcta tgcccgacgg cgaggatctc gtcgtgaccc
     5221 acggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     5281 actgtggccg tctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     5341 ttgctgaaga gcttggcggc gaatgggctg accgcttcct tgtgctttac ggtatcgccg
     5401 cgcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaccgattc
     5461 taggtgcatt ggcgcagaaa aaaatgcctg atgcgacgct gcgcgtctta tactcccaca
     5521 tatgccagat tcagcaacgg atacggcttc cccaacttgc ccacttccat acgtgtcctc
     5581 cttaccagaa atttatcctt aaggtcgttt aaactcgact ctggctctat cgaatctccg
     5641 tcgtttcgag cttacgcgaa cagccgtggc gctcatttgc tcgtcgggca tcgaatctcg
     5701 tcagctatcg tcagcttacc tttttggca
//
