LOCUS       pSF-CMV-RSV-FLuc        6443 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector for co-expressing a gene together with firefly 
            luciferase expressed from the Rous sarcoma virus promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pSF-CMV-RSV-FLuc AscI
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6443)
  AUTHORS   Oxford Genetics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6443)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..89
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     terminator      complement(94..141)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    complement(158..206)
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     enhancer        287..590
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        591..794
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    857..942
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     RBS             893..898
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     misc_signal     903..909
                     /label=Kozak sequence
                     /note="Kozak sequence"
     CDS             907..909
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     misc_feature    961..971
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     misc_feature    1009..1019
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    complement(1051..1172)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     terminator      1286..1333
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      1363..1499
                     /note="rrnG terminator"
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    1544..1615
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     rep_origin      complement(1841..2429)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3110..3372
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     CDS             3385..5034
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    5059..5107
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     RBS             5360..5365
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             5373..6164
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      6180..6316
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 gcgatcgcgg ctcccgacat cttggaccat tagctccaca ggtatcttct tccctctagt
       61 ggtcataaca gcagcttcag ctacctctca attcaaaaaa cccctcaaga cccgtttaga
      121 ggccccaagg ggttatgcta tcaatcgttg cgttacacac acaaaaaacc aacacacatc
      181 catcttcgat ggatagcgat tttattatct aactgctgat cgagtgtagc cagatctagt
      241 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      301 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      361 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      421 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      481 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      541 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg ctgatgcggt
      601 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      661 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      721 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      781 atataagcag agctggttta gtgaaccgtc agatcagatc tttgtcgatc ctaccatcca
      841 ctcgacacac ccgccagcgg ccgctgccaa gcttccgagc tctcgaattc aaaggaggta
      901 cccaccatgg ggtaccgcga tatctacctc gaggtttcta gaagttgtct cctcctgcac
      961 tgactgactg atacaatcga tttctggatc cgcaggcctc tgctagcttg actgactgag
     1021 atacagcgta ccttcagctc acagacatga taagatacat tgatgagttt ggacaaacca
     1081 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     1141 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     1201 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     1261 gtattggccc atctctatcg gtatcgtagc ataacccctt ggggcctcta aacgggtctt
     1321 gaggggtttt ttgtgcccct cgggccggat tgctatctac cggcattggc gcagaaaaaa
     1381 atgcctgatg cgacgctgcg cgtcttatac tcccacatat gccagattca gcaacggata
     1441 cggcttcccc aacttgccca cttccatacg tgtcctcctt accagaaatt tatccttaag
     1501 gtcgtcagct atcctgcagg cgatctctcg atttcgatca agacattcct ttaatggtct
     1561 tttctggaca ccactagggg tcagaagtag ttcatcaaac tttcttccct ccctaatctc
     1621 attggttacc ttgggctatc gaaacttaat taaccagtca agtcagctac ttggcgagat
     1681 cgacttgtct gggtttcgac tacgctcaga attgcgtcag tcaagttcga tctggtcctt
     1741 gctattgcac ccgttctccg attacgagtt tcatttaaat catgtgagca aaaggccagc
     1801 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     1861 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     1921 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     1981 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2041 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2101 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2161 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     2221 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2281 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     2341 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2401 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     2461 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     2521 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     2581 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     2641 gtctatttcg ttcatccata gttgcattta aatttccgaa ctctccaagg ccctcgtcgg
     2701 aaaatcttca aacctttcgt ccgatccatc ttgcaggcta cctctcgaac gaactatcgc
     2761 aagtctcttg gccggccttg cgccttggct attgcttggc agcgcctatc gccaggtatt
     2821 actccaatcc cgaatatccg agatcgggat cacccgagag aagttcaacc tacatcctca
     2881 atcccgatct atccgagatc cgaggaatat cgaaatcggg gcgcgcccga caattgcatg
     2941 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
     3001 cgtatctgag gggactaggg tgtgtttagg cgaaaagcgg ggcttcggtt gtacgcggtt
     3061 aggagtcccc tcaggatata gtagtttcgc ttttgcatag ggagggggaa atgtagtctt
     3121 atgcaataca cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag
     3181 gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat
     3241 taggaaggca acagacaggt ctgacatgga ttggacgaac cactgaattg cgcattgcag
     3301 agataattgt atttaagtgc ctagctcgat acaataaacg ccatttgacc attcaccaca
     3361 ttggtgtgca cctccaaggc caacatggaa gatgccaaaa acattaagaa aggcccagcg
     3421 ccattctacc cactcgaaga cgggaccgct ggcgagcagc tgcataaagc catgaagcgc
     3481 tacgccctgg tgcccggcac catcgccttt accgacgcac atatcgaggt ggacattacc
     3541 tacgccgagt acttcgagat gagcgttcgg ctggcagaag ctatgaagcg ctatgggctg
     3601 aatacaaacc atcggatcgt ggtgtgtagc gagaatagct tgcagttctt catgcccgtg
     3661 ttgggtgccc tgttcatcgg tgtggctgtg gccccagcta acgacatcta caacgagcgc
     3721 gagctgctga acagcatggg catcagccag cccaccgtcg tattcgtgag caagaaaggg
     3781 ctgcaaaaga tcctcaacgt gcaaaagaag ctaccgatca tacaaaagat catcatcatg
     3841 gatagcaaga ccgactacca gggcttccaa agcatgtaca ccttcgtgac ttcccatttg
     3901 ccacccggct tcaacgagta cgacttcgtg cccgagagct tcgaccggga caaaaccatc
     3961 gccctgatca tgaacagtag tggcagtacc ggattgccca agggcgtagc cctaccgcac
     4021 cgcaccgctt gtgtccgatt cagtcatgcc cgcgacccca tcttcggcaa ccagatcatc
     4081 cccgacaccg ctatcctgag cgtggtgcca tttcaccacg gcttcggcat gttcaccacg
     4141 ctgggctact tgatctgcgg ctttcgggtc gtgctcatgt accgcttcga ggaagagcta
     4201 ttcttgcgca gcttgcaaga ctataagatt caatctgccc tgctggtgcc cacactattt
     4261 agcttcttcg ctaagagcac tctcatcgac aagtacgacc taagcaactt gcacgagatc
     4321 gccagcggcg gggcgccgct cagcaaggag gtaggtgagg ccgtggccaa acgcttccac
     4381 ctaccaggca tccgccaggg ctacggcctg acagaaacaa ccagcgccat tctgatcacc
     4441 cccgaagggg acgacaagcc tggcgcagta ggcaaggtgg tgcccttctt cgaggctaag
     4501 gtggtggact tggacaccgg taagacactg ggtgtgaacc agcgcggcga gctgtgcgtc
     4561 cgtggcccca tgatcatgag cggctacgtt aacaaccccg aggctacaaa cgctctcatc
     4621 gacaaggacg gctggctgca tagcggcgac atcgcctact gggacgagga cgagcacttc
     4681 ttcatcgtgg accggctgaa gagcctgatc aaatacaagg gctaccaggt agccccagcc
     4741 gaactggaga gcatcctgct gcaacacccc aacatcttcg acgccggggt cgccggcctg
     4801 cccgacgacg atgccggcga gctgcccgcc gcagtcgtcg tgctggaaca cggtaaaacc
     4861 atgaccgaga aggagatcgt ggactatgtg gccagccagg ttacaaccgc caagaagctg
     4921 cgcggtggtg ttgtgttcgt ggacgaggtg cctaaaggac tgaccggcaa gttggacgcc
     4981 cgcaagatcc gcgagattct cattaaggcc aagaagggcg gcaagatcgc cgtctagtat
     5041 gtaagcctag tcttagataa taaaatcgct atccatcgaa gatggatgtg tgttggtttt
     5101 ttgtgtgtgt aacgctaggc gcgcctggtg taccgagaac gatcctctca gtgcgagtct
     5161 cgacgatcca tatcgttgct tggcagtcag ccagtcggaa tccagcttgg gacccaggaa
     5221 gtccaatcgt cagatattgt actcaagcct ggtcacggca gcgtaccgat ctgtttaaac
     5281 ctagatattg atagtctgat cggtcaacgt ataatcgagt cctagctttt gcaaacatct
     5341 atcaagagac aggatcagca ggaggctttc gcatgattga acaagatgga ttgcacgcag
     5401 gttctccggc ggcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     5461 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgtccggtt ctttttgtca
     5521 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
     5581 tggcgacgac gggcgttcct tgcgcggctg tgctcgacgt tgtcactgaa gcgggaaggg
     5641 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     5701 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     5761 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     5821 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     5881 tgttcgccag gctcaaggcg tctatgcccg acggcgagga tctcgtcgtg acccacggcg
     5941 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     6001 gccgtctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     6061 aagagcttgg cggcgaatgg gctgaccgct tccttgtgct ttacggtatc gccgcgcccg
     6121 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaccg attctaggtg
     6181 cattggcgca gaaaaaaatg cctgatgcga cgctgcgcgt cttatactcc cacatatgcc
     6241 agattcagca acggatacgg cttccccaac ttgcccactt ccatacgtgt cctccttacc
     6301 agaaatttat ccttaaggtc gtttaaactc gactctggct ctatcgaatc tccgtcgttt
     6361 cgagcttacg cgaacagccg tggcgctcat ttgctcgtcg ggcatcgaat ctcgtcagct
     6421 atcgtcagct tacctttttg gca
//
