LOCUS       pSF-CMV-RLuc.        5222 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector for strong constitutive expression of Renilla 
            luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pSF-CMV-RLuc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Oxford Genetics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5222)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..89
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     terminator      complement(94..141)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    complement(158..206)
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     enhancer        287..590
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        591..794
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     RBS             893..898
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             907..1842
                     /label=RLuc
                     /note="Renilla luciferase"
     misc_feature    1865..1875
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     misc_feature    1913..1923
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    complement(1955..2076)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     terminator      2190..2237
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      2267..2403
                     /note="rrnG terminator"
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    2448..2519
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     rep_origin      complement(2745..3333)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     RBS             4066..4071
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             4079..4936
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      4952..5088
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 gcgatcgcgg ctcccgacat cttggaccat tagctccaca ggtatcttct tccctctagt
       61 ggtcataaca gcagcttcag ctacctctca attcaaaaaa cccctcaaga cccgtttaga
      121 ggccccaagg ggttatgcta tcaatcgttg cgttacacac acaaaaaacc aacacacatc
      181 catcttcgat ggatagcgat tttattatct aactgctgat cgagtgtagc cagatctagt
      241 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      301 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      361 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      421 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      481 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      541 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg ctgatgcggt
      601 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      661 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      721 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      781 atataagcag agctggttta gtgaaccgtc agatcagatc tttgtcgatc ctaccatcca
      841 ctcgacacac ccgccagcgg ccgctgccaa gcttccgagc tctcgaattc aaaggaggta
      901 cccaccatga ccagcaaagt gtatgatccg gaacagcgca aacgcatgat taccggcccg
      961 cagtggtggg cgcgctgcaa acagatgaac gtgctggata gctttattaa ctattatgat
     1021 agcgaaaaac atgcggaaaa cgcggtgatt tttctgcatg gcaacgcggc gagcagctat
     1081 ctgtggcggc atgtggtgcc gcatattgaa ccagtggcgc gctgcattat tccggatctg
     1141 attggcatgg gcaaaagcgg caaaagcggc aacggcagct atcgcctgct ggatcattat
     1201 aaatatctga ccgcgtggtt tgaactgctg aacctgccga aaaaaattat ttttgtgggc
     1261 catgattggg gcgcgtgcct ggcgtttcat tatagctatg aacatcagga taaaattaaa
     1321 gcgattgtgc acgcggaaag cgtggtggat gtgattgaaa gctgggatga atggccggat
     1381 attgaagaag atattgcgct gattaaaagc gaagaaggcg aaaaaatggt gctggaaaac
     1441 aacttttttg tggaaaccat gctgccgagc aaaattatgc gcaaactgga accggaagaa
     1501 tttgcggcgt atctggaacc gtttaaagaa aaaggcgaag tgcgccgccc gaccctgagc
     1561 tggccgcgcg aaattccgct ggtgaaaggc ggcaaaccgg atgtggtcca gattgtgcgc
     1621 aactataacg cgtatctgcg cgcgagcgat gatctgccga aaatgtttat tgaaagcgat
     1681 ccgggctttt ttagcaacgc gattgtggaa ggcgcgaaaa aatttccgaa caccgaattt
     1741 gtgaaagtga agggcctgca ttttagccag gaagatgcgc cggatgaaat gggcaaatat
     1801 attaagagct ttgtggaacg cgtgctgaaa aacgaacagg gctagaagtt gtctcctcct
     1861 gcactgactg actgatacaa tcgatttctg gatccgcagg cctctgctag cttgactgac
     1921 tgagatacag cgtaccttca gctcacagac atgataagat acattgatga gtttggacaa
     1981 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     2041 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     2101 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     2161 tgtggtattg gcccatctct atcggtatcg tagcataacc ccttggggcc tctaaacggg
     2221 tcttgagggg ttttttgtgc ccctcgggcc ggattgctat ctaccggcat tggcgcagaa
     2281 aaaaatgcct gatgcgacgc tgcgcgtctt atactcccac atatgccaga ttcagcaacg
     2341 gatacggctt ccccaacttg cccacttcca tacgtgtcct ccttaccaga aatttatcct
     2401 taaggtcgtc agctatcctg caggcgatct ctcgatttcg atcaagacat tcctttaatg
     2461 gtcttttctg gacaccacta ggggtcagaa gtagttcatc aaactttctt ccctccctaa
     2521 tctcattggt taccttgggc tatcgaaact taattaacca gtcaagtcag ctacttggcg
     2581 agatcgactt gtctgggttt cgactacgct cagaattgcg tcagtcaagt tcgatctggt
     2641 ccttgctatt gcacccgttc tccgattacg agtttcattt aaatcatgtg agcaaaaggc
     2701 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     2761 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     2821 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     2881 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     2941 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     3001 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     3061 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     3121 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     3181 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3241 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3301 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     3361 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3421 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     3481 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     3541 atctgtctat ttcgttcatc catagttgca tttaaatttc cgaactctcc aaggccctcg
     3601 tcggaaaatc ttcaaacctt tcgtccgatc catcttgcag gctacctctc gaacgaacta
     3661 tcgcaagtct cttggccggc cttgcgcctt ggctattgct tggcagcgcc tatcgccagg
     3721 tattactcca atcccgaata tccgagatcg ggatcacccg agagaagttc aacctacatc
     3781 ctcaatcccg atctatccga gatccgagga atatcgaaat cggggcgcgc ctggtgtacc
     3841 gagaacgatc ctctcagtgc gagtctcgac gatccatatc gttgcttggc agtcagccag
     3901 tcggaatcca gcttgggacc caggaagtcc aatcgtcaga tattgtactc aagcctggtc
     3961 acggcagcgt accgatctgt ttaaacctag atattgatag tctgatcggt caacgtataa
     4021 tcgagtccta gcttttgcaa acatctatca agagacagga tcagcaggag gctttcgcat
     4081 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     4141 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcgcg
     4201 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     4261 agaacgcttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     4321 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     4381 tgagtattca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     4441 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgattgg
     4501 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     4561 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     4621 tgtagcaatg gcaacaacct tgcgtaaact attaactggc gaactactta ctctagcttc
     4681 ccggcaacag ttgatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     4741 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     4801 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     4861 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     4921 actgattaag cattggtaac cgattctagg tgcattggcg cagaaaaaaa tgcctgatgc
     4981 gacgctgcgc gtcttatact cccacatatg ccagattcag caacggatac ggcttcccca
     5041 acttgcccac ttccatacgt gtcctcctta ccagaaattt atccttaaga tcccgaatcg
     5101 tttaaactcg actctggctc tatcgaatct ccgtcgtttc gagcttacgc gaacagccgt
     5161 ggcgctcatt tgctcgtcgg gcatcgaatc tcgtcagcta tcgtcagctt acctttttgg
     5221 ca
//
