LOCUS       pSF-CMV-FMDV-RLu        5650 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector for co-expressing a gene together with Renilla 
            luciferase expressed from the foot-and-mouth disease virus IRES.
ACCESSION   .
VERSION     .
KEYWORDS    pSF-CMV-FMDV-RLuc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Oxford Genetics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5650)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..89
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     terminator      complement(94..141)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    complement(158..206)
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     enhancer        287..590
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        591..794
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    857..942
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     RBS             893..898
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     misc_signal     903..909
                     /label=Kozak sequence
                     /note="Kozak sequence"
     CDS             907..909
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     misc_feature    961..971
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     misc_feature    1012..1457
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the
                     foot-and-mouth disease virus"
     CDS             1473..2408
                     /label=RLuc
                     /note="Renilla luciferase"
     misc_feature    2414..2424
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    complement(2456..2577)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     terminator      2691..2738
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      2768..2904
                     /note="rrnG terminator"
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    2949..3020
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     rep_origin      complement(3246..3834)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     RBS             4567..4572
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             4580..5371
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      5387..5523
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 gcgatcgcgg ctcccgacat cttggaccat tagctccaca ggtatcttct tccctctagt
       61 ggtcataaca gcagcttcag ctacctctca attcaaaaaa cccctcaaga cccgtttaga
      121 ggccccaagg ggttatgcta tcaatcgttg cgttacacac acaaaaaacc aacacacatc
      181 catcttcgat ggatagcgat tttattatct aactgctgat cgagtgtagc cagatctagt
      241 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      301 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      361 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      421 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      481 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      541 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg ctgatgcggt
      601 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      661 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      721 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      781 atataagcag agctggttta gtgaaccgtc agatcagatc tttgtcgatc ctaccatcca
      841 ctcgacacac ccgccagcgg ccgctgccaa gcttccgagc tctcgaattc aaaggaggta
      901 cccaccatgg ggtaccgcga tatctacctc gaggtttcta gaagttgtct cctcctgcac
      961 tgactgactg atacaatcga tatgccaaaa gcaaagcgct atcgcgcctt aagcaggttt
     1021 ccccaactga cacaaaacgt gcaacttgaa actccgcctg gtctttccag gtctagaggg
     1081 gtaacacttt gtactgcgtt tggctccacg ctcgatccac tggcgagtgt tagtaacagc
     1141 actgttgctt cgtagcggag catgacggcc gtgggaactc ctccttggta acaaggaccc
     1201 acggggccaa aagccacgcc cacacgggcc cgtcatgtgt gcaaccccag cacggcgact
     1261 ttactgcgaa acccacttta aagtgacatt gaaactggta cccacacact ggtgacaggc
     1321 taaggatgcc cttcaggtac cccgaggtaa cacgcgacac tcgggatctg agaaggggac
     1381 tggggcttct ataaaagcgc tcggtttaaa aagcttctat gcctgaatag gtgaccggag
     1441 gtcggcacct ttcctttgca attactgacc acatgaccag caaagtgtat gatccggaac
     1501 agcgcaaacg catgattacc ggcccgcagt ggtgggcgcg ctgcaaacag atgaacgtgc
     1561 tggatagctt tattaactat tatgatagcg aaaaacatgc ggaaaacgcg gtgatttttc
     1621 tgcatggcaa cgcggcgagc agctatctgt ggcggcatgt ggtgccgcat attgaaccag
     1681 tggcgcgctg cattattccg gatctgattg gcatgggcaa aagcggcaaa agcggcaacg
     1741 gcagctatcg cctgctggat cattataaat atctgaccgc gtggtttgaa ctgctgaacc
     1801 tgccgaaaaa aattattttt gtgggccatg attggggcgc gtgcctggcg tttcattata
     1861 gctatgaaca tcaggataaa attaaagcga ttgtgcacgc ggaaagcgtg gtggatgtga
     1921 ttgaaagctg ggatgaatgg ccggatattg aagaagatat tgcgctgatt aaaagcgaag
     1981 aaggcgaaaa aatggtgctg gaaaacaact tttttgtgga aaccatgctg ccgagcaaaa
     2041 ttatgcgcaa actggaaccg gaagaatttg cggcgtatct ggaaccgttt aaagaaaaag
     2101 gcgaagtgcg ccgcccgacc ctgagctggc cgcgcgaaat tccgctggtg aaaggcggca
     2161 aaccggatgt ggtccagatt gtgcgcaact ataacgcgta tctgcgcgcg agcgatgatc
     2221 tgccgaaaat gtttattgaa agcgatccgg gcttttttag caacgcgatt gtggaaggcg
     2281 cgaaaaaatt tccgaacacc gaatttgtga aagtgaaggg cctgcatttt agccaggaag
     2341 atgcgccgga tgaaatgggc aaatatatta agagctttgt ggaacgcgtg ctgaaaaacg
     2401 aacagggcta gcttgactga ctgagataca gcgtaccttc agctcacaga catgataaga
     2461 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     2521 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     2581 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     2641 agcaagtaaa acctctacaa atgtggtatt ggcccatctc tatcggtatc gtagcataac
     2701 cccttggggc ctctaaacgg gtcttgaggg gttttttgtg cccctcgggc cggattgcta
     2761 tctaccggca ttggcgcaga aaaaaatgcc tgatgcgacg ctgcgcgtct tatactccca
     2821 catatgccag attcagcaac ggatacggct tccccaactt gcccacttcc atacgtgtcc
     2881 tccttaccag aaatttatcc ttaaggtcgt cagctatcct gcaggcgatc tctcgatttc
     2941 gatcaagaca ttcctttaat ggtcttttct ggacaccact aggggtcaga agtagttcat
     3001 caaactttct tccctcccta atctcattgg ttaccttggg ctatcgaaac ttaattaacc
     3061 agtcaagtca gctacttggc gagatcgact tgtctgggtt tcgactacgc tcagaattgc
     3121 gtcagtcaag ttcgatctgg tccttgctat tgcacccgtt ctccgattac gagtttcatt
     3181 taaatcatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     3241 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     3301 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     3361 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3421 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3481 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3541 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3601 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3661 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     3721 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3781 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3841 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3901 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3961 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     4021 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc atttaaattt
     4081 ccgaactctc caaggccctc gtcggaaaat cttcaaacct ttcgtccgat ccatcttgca
     4141 ggctacctct cgaacgaact atcgcaagtc tcttggccgg ccttgcgcct tggctattgc
     4201 ttggcagcgc ctatcgccag gtattactcc aatcccgaat atccgagatc gggatcaccc
     4261 gagagaagtt caacctacat cctcaatccc gatctatccg agatccgagg aatatcgaaa
     4321 tcggggcgcg cctggtgtac cgagaacgat cctctcagtg cgagtctcga cgatccatat
     4381 cgttgcttgg cagtcagcca gtcggaatcc agcttgggac ccaggaagtc caatcgtcag
     4441 atattgtact caagcctggt cacggcagcg taccgatctg tttaaaccta gatattgata
     4501 gtctgatcgg tcaacgtata atcgagtcct agcttttgca aacatctatc aagagacagg
     4561 atcagcagga ggctttcgca tgattgaaca agatggattg cacgcaggtt ctccggcggc
     4621 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     4681 cgccgtgttc cggctgtcag cgcaggggcg tccggttctt tttgtcaaga ccgacctgtc
     4741 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg cgacgacggg
     4801 cgttccttgc gcggctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     4861 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     4921 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     4981 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     5041 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     5101 caaggcgtct atgcccgacg gcgaggatct cgtcgtgacc cacggcgatg cctgcttgcc
     5161 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc gtctgggtgt
     5221 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     5281 cgaatgggct gaccgcttcc ttgtgcttta cggtatcgcc gcgcccgatt cgcagcgcat
     5341 cgccttctat cgccttcttg acgagttctt ctgaccgatt ctaggtgcat tggcgcagaa
     5401 aaaaatgcct gatgcgacgc tgcgcgtctt atactcccac atatgccaga ttcagcaacg
     5461 gatacggctt ccccaacttg cccacttcca tacgtgtcct ccttaccaga aatttatcct
     5521 taaggtcgtt taaactcgac tctggctcta tcgaatctcc gtcgtttcga gcttacgcga
     5581 acagccgtgg cgctcatttg ctcgtcgggc atcgaatctc gtcagctatc gtcagcttac
     5641 ctttttggca
//
