LOCUS       pSF-CMV-FMDV-FLu        6369 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector for co-expressing a gene together with firefly 
            luciferase expressed from the foot-and-mouth disease virus IRES.
ACCESSION   .
VERSION     .
KEYWORDS    pSF-CMV-FMDV-FLuc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Oxford Genetics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..89
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     terminator      complement(94..141)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    complement(158..206)
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     enhancer        287..590
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        591..794
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    857..942
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     RBS             893..898
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     misc_signal     903..909
                     /label=Kozak sequence
                     /note="Kozak sequence"
     CDS             907..909
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     misc_feature    961..971
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     misc_feature    1012..1457
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the
                     foot-and-mouth disease virus"
     CDS             1473..3122
                     /label=luciferase
                     /note="firefly luciferase"
     misc_feature    3133..3143
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    complement(3175..3296)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     terminator      3410..3457
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      3487..3623
                     /note="rrnG terminator"
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    3668..3739
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     rep_origin      complement(3965..4553)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     RBS             5286..5291
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             5299..6090
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      6106..6242
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 gcgatcgcgg ctcccgacat cttggaccat tagctccaca ggtatcttct tccctctagt
       61 ggtcataaca gcagcttcag ctacctctca attcaaaaaa cccctcaaga cccgtttaga
      121 ggccccaagg ggttatgcta tcaatcgttg cgttacacac acaaaaaacc aacacacatc
      181 catcttcgat ggatagcgat tttattatct aactgctgat cgagtgtagc cagatctagt
      241 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      301 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      361 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      421 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      481 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      541 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg ctgatgcggt
      601 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      661 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      721 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      781 atataagcag agctggttta gtgaaccgtc agatcagatc tttgtcgatc ctaccatcca
      841 ctcgacacac ccgccagcgg ccgctgccaa gcttccgagc tctcgaattc aaaggaggta
      901 cccaccatgg ggtaccgcga tatctacctc gaggtttcta gaagttgtct cctcctgcac
      961 tgactgactg atacaatcga tatgccaaaa gcaaagcgct atcgcgcctt aagcaggttt
     1021 ccccaactga cacaaaacgt gcaacttgaa actccgcctg gtctttccag gtctagaggg
     1081 gtaacacttt gtactgcgtt tggctccacg ctcgatccac tggcgagtgt tagtaacagc
     1141 actgttgctt cgtagcggag catgacggcc gtgggaactc ctccttggta acaaggaccc
     1201 acggggccaa aagccacgcc cacacgggcc cgtcatgtgt gcaaccccag cacggcgact
     1261 ttactgcgaa acccacttta aagtgacatt gaaactggta cccacacact ggtgacaggc
     1321 taaggatgcc cttcaggtac cccgaggtaa cacgcgacac tcgggatctg agaaggggac
     1381 tggggcttct ataaaagcgc tcggtttaaa aagcttctat gcctgaatag gtgaccggag
     1441 gtcggcacct ttcctttgca attactgacc acatggaaga tgccaaaaac attaagaaag
     1501 gcccagcgcc attctaccca ctcgaagacg ggaccgctgg cgagcagctg cataaagcca
     1561 tgaagcgcta cgccctggtg cccggcacca tcgcctttac cgacgcacat atcgaggtgg
     1621 acattaccta cgccgagtac ttcgagatga gcgttcggct ggcagaagct atgaagcgct
     1681 atgggctgaa tacaaaccat cggatcgtgg tgtgtagcga gaatagcttg cagttcttca
     1741 tgcccgtgtt gggtgccctg ttcatcggtg tggctgtggc cccagctaac gacatctaca
     1801 acgagcgcga gctgctgaac agcatgggca tcagccagcc caccgtcgta ttcgtgagca
     1861 agaaagggct gcaaaagatc ctcaacgtgc aaaagaagct accgatcata caaaagatca
     1921 tcatcatgga tagcaagacc gactaccagg gcttccaaag catgtacacc ttcgtgactt
     1981 cccatttgcc acccggcttc aacgagtacg acttcgtgcc cgagagcttc gaccgggaca
     2041 aaaccatcgc cctgatcatg aacagtagtg gcagtaccgg attgcccaag ggcgtagccc
     2101 taccgcaccg caccgcttgt gtccgattca gtcatgcccg cgaccccatc ttcggcaacc
     2161 agatcatccc cgacaccgct atcctgagcg tggtgccatt tcaccacggc ttcggcatgt
     2221 tcaccacgct gggctacttg atctgcggct ttcgggtcgt gctcatgtac cgcttcgagg
     2281 aagagctatt cttgcgcagc ttgcaagact ataagattca atctgccctg ctggtgccca
     2341 cactatttag cttcttcgct aagagcactc tcatcgacaa gtacgaccta agcaacttgc
     2401 acgagatcgc cagcggcggg gcgccgctca gcaaggaggt aggtgaggcc gtggccaaac
     2461 gcttccacct accaggcatc cgccagggct acggcctgac agaaacaacc agcgccattc
     2521 tgatcacccc cgaaggggac gacaagcctg gcgcagtagg caaggtggtg cccttcttcg
     2581 aggctaaggt ggtggacttg gacaccggta agacactggg tgtgaaccag cgcggcgagc
     2641 tgtgcgtccg tggccccatg atcatgagcg gctacgttaa caaccccgag gctacaaacg
     2701 ctctcatcga caaggacggc tggctgcata gcggcgacat cgcctactgg gacgaggacg
     2761 agcacttctt catcgtggac cggctgaaga gcctgatcaa atacaagggc taccaggtag
     2821 ccccagccga actggagagc atcctgctgc aacaccccaa catcttcgac gccggggtcg
     2881 ccggcctgcc cgacgacgat gccggcgagc tgcccgccgc agtcgtcgtg ctggaacacg
     2941 gtaaaaccat gaccgagaag gagatcgtgg actatgtggc cagccaggtt acaaccgcca
     3001 agaagctgcg cggtggtgtt gtgttcgtgg acgaggtgcc taaaggactg accggcaagt
     3061 tggacgcccg caagatccgc gagattctca ttaaggccaa gaagggcggc aagatcgccg
     3121 tctaggctag cttgactgac tgagatacag cgtaccttca gctcacagac atgataagat
     3181 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     3241 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     3301 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     3361 gcaagtaaaa cctctacaaa tgtggtattg gcccatctct atcggtatcg tagcataacc
     3421 ccttggggcc tctaaacggg tcttgagggg ttttttgtgc ccctcgggcc ggattgctat
     3481 ctaccggcat tggcgcagaa aaaaatgcct gatgcgacgc tgcgcgtctt atactcccac
     3541 atatgccaga ttcagcaacg gatacggctt ccccaacttg cccacttcca tacgtgtcct
     3601 ccttaccaga aatttatcct taaggtcgtc agctatcctg caggcgatct ctcgatttcg
     3661 atcaagacat tcctttaatg gtcttttctg gacaccacta ggggtcagaa gtagttcatc
     3721 aaactttctt ccctccctaa tctcattggt taccttgggc tatcgaaact taattaacca
     3781 gtcaagtcag ctacttggcg agatcgactt gtctgggttt cgactacgct cagaattgcg
     3841 tcagtcaagt tcgatctggt ccttgctatt gcacccgttc tccgattacg agtttcattt
     3901 aaatcatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     3961 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     4021 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     4081 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     4141 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     4201 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     4261 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     4321 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     4381 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     4441 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     4501 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     4561 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     4621 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     4681 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     4741 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgca tttaaatttc
     4801 cgaactctcc aaggccctcg tcggaaaatc ttcaaacctt tcgtccgatc catcttgcag
     4861 gctacctctc gaacgaacta tcgcaagtct cttggccggc cttgcgcctt ggctattgct
     4921 tggcagcgcc tatcgccagg tattactcca atcccgaata tccgagatcg ggatcacccg
     4981 agagaagttc aacctacatc ctcaatcccg atctatccga gatccgagga atatcgaaat
     5041 cggggcgcgc ctggtgtacc gagaacgatc ctctcagtgc gagtctcgac gatccatatc
     5101 gttgcttggc agtcagccag tcggaatcca gcttgggacc caggaagtcc aatcgtcaga
     5161 tattgtactc aagcctggtc acggcagcgt accgatctgt ttaaacctag atattgatag
     5221 tctgatcggt caacgtataa tcgagtccta gcttttgcaa acatctatca agagacagga
     5281 tcagcaggag gctttcgcat gattgaacaa gatggattgc acgcaggttc tccggcggct
     5341 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     5401 gccgtgttcc ggctgtcagc gcaggggcgt ccggttcttt ttgtcaagac cgacctgtcc
     5461 ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc gacgacgggc
     5521 gttccttgcg cggctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     5581 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     5641 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     5701 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     5761 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     5821 aaggcgtcta tgcccgacgg cgaggatctc gtcgtgaccc acggcgatgc ctgcttgccg
     5881 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg tctgggtgtg
     5941 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     6001 gaatgggctg accgcttcct tgtgctttac ggtatcgccg cgcccgattc gcagcgcatc
     6061 gccttctatc gccttcttga cgagttcttc tgaccgattc taggtgcatt ggcgcagaaa
     6121 aaaatgcctg atgcgacgct gcgcgtctta tactcccaca tatgccagat tcagcaacgg
     6181 atacggcttc cccaacttgc ccacttccat acgtgtcctc cttaccagaa atttatcctt
     6241 aaggtcgttt aaactcgact ctggctctat cgaatctccg tcgtttcgag cttacgcgaa
     6301 cagccgtggc gctcatttgc tcgtcgggca tcgaatctcg tcagctatcg tcagcttacc
     6361 tttttggca
//
