LOCUS       pSF-CMV-FLuc.        5936 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector for strong constitutive expression of firefly 
            luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pSF-CMV-FLuc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Oxford Genetics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5936)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5936
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..89
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     terminator      complement(94..141)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    complement(158..206)
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     enhancer        287..590
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        591..794
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     RBS             893..898
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             907..2556
                     /label=luciferase
                     /note="firefly luciferase"
     misc_feature    2579..2589
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     misc_feature    2627..2637
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    complement(2669..2790)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     terminator      2904..2951
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      2981..3117
                     /note="rrnG terminator"
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    3162..3233
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     rep_origin      complement(3459..4047)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     RBS             4780..4785
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             4793..5650
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      5666..5802
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 gcgatcgcgg ctcccgacat cttggaccat tagctccaca ggtatcttct tccctctagt
       61 ggtcataaca gcagcttcag ctacctctca attcaaaaaa cccctcaaga cccgtttaga
      121 ggccccaagg ggttatgcta tcaatcgttg cgttacacac acaaaaaacc aacacacatc
      181 catcttcgat ggatagcgat tttattatct aactgctgat cgagtgtagc cagatctagt
      241 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      301 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      361 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      421 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      481 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      541 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg ctgatgcggt
      601 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      661 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      721 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      781 atataagcag agctggttta gtgaaccgtc agatcagatc tttgtcgatc ctaccatcca
      841 ctcgacacac ccgccagcgg ccgctgccaa gcttccgagc tctcgaattc aaaggaggta
      901 cccaccatgg aagatgccaa aaacattaag aaaggcccag cgccattcta cccactcgaa
      961 gacgggaccg ctggcgagca gctgcataaa gccatgaagc gctacgccct ggtgcccggc
     1021 accatcgcct ttaccgacgc acatatcgag gtggacatta cctacgccga gtacttcgag
     1081 atgagcgttc ggctggcaga agctatgaag cgctatgggc tgaatacaaa ccatcggatc
     1141 gtggtgtgta gcgagaatag cttgcagttc ttcatgcccg tgttgggtgc cctgttcatc
     1201 ggtgtggctg tggccccagc taacgacatc tacaacgagc gcgagctgct gaacagcatg
     1261 ggcatcagcc agcccaccgt cgtattcgtg agcaagaaag ggctgcaaaa gatcctcaac
     1321 gtgcaaaaga agctaccgat catacaaaag atcatcatca tggatagcaa gaccgactac
     1381 cagggcttcc aaagcatgta caccttcgtg acttcccatt tgccacccgg cttcaacgag
     1441 tacgacttcg tgcccgagag cttcgaccgg gacaaaacca tcgccctgat catgaacagt
     1501 agtggcagta ccggattgcc caagggcgta gccctaccgc accgcaccgc ttgtgtccga
     1561 ttcagtcatg cccgcgaccc catcttcggc aaccagatca tccccgacac cgctatcctg
     1621 agcgtggtgc catttcacca cggcttcggc atgttcacca cgctgggcta cttgatctgc
     1681 ggctttcggg tcgtgctcat gtaccgcttc gaggaagagc tattcttgcg cagcttgcaa
     1741 gactataaga ttcaatctgc cctgctggtg cccacactat ttagcttctt cgctaagagc
     1801 actctcatcg acaagtacga cctaagcaac ttgcacgaga tcgccagcgg cggggcgccg
     1861 ctcagcaagg aggtaggtga ggccgtggcc aaacgcttcc acctaccagg catccgccag
     1921 ggctacggcc tgacagaaac aaccagcgcc attctgatca cccccgaagg ggacgacaag
     1981 cctggcgcag taggcaaggt ggtgcccttc ttcgaggcta aggtggtgga cttggacacc
     2041 ggtaagacac tgggtgtgaa ccagcgcggc gagctgtgcg tccgtggccc catgatcatg
     2101 agcggctacg ttaacaaccc cgaggctaca aacgctctca tcgacaagga cggctggctg
     2161 catagcggcg acatcgccta ctgggacgag gacgagcact tcttcatcgt ggaccggctg
     2221 aagagcctga tcaaatacaa gggctaccag gtagccccag ccgaactgga gagcatcctg
     2281 ctgcaacacc ccaacatctt cgacgccggg gtcgccggcc tgcccgacga cgatgccggc
     2341 gagctgcccg ccgcagtcgt cgtgctggaa cacggtaaaa ccatgaccga gaaggagatc
     2401 gtggactatg tggccagcca ggttacaacc gccaagaagc tgcgcggtgg tgttgtgttc
     2461 gtggacgagg tgcctaaagg actgaccggc aagttggacg cccgcaagat ccgcgagatt
     2521 ctcattaagg ccaagaaggg cggcaagatc gccgtctaga agttgtctcc tcctgcactg
     2581 actgactgat acaatcgatt tctggatccg caggcctctg ctagcttgac tgactgagat
     2641 acagcgtacc ttcagctcac agacatgata agatacattg atgagtttgg acaaaccaca
     2701 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     2761 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     2821 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     2881 attggcccat ctctatcggt atcgtagcat aaccccttgg ggcctctaaa cgggtcttga
     2941 ggggtttttt gtgcccctcg ggccggattg ctatctaccg gcattggcgc agaaaaaaat
     3001 gcctgatgcg acgctgcgcg tcttatactc ccacatatgc cagattcagc aacggatacg
     3061 gcttccccaa cttgcccact tccatacgtg tcctccttac cagaaattta tccttaaggt
     3121 cgtcagctat cctgcaggcg atctctcgat ttcgatcaag acattccttt aatggtcttt
     3181 tctggacacc actaggggtc agaagtagtt catcaaactt tcttccctcc ctaatctcat
     3241 tggttacctt gggctatcga aacttaatta accagtcaag tcagctactt ggcgagatcg
     3301 acttgtctgg gtttcgacta cgctcagaat tgcgtcagtc aagttcgatc tggtccttgc
     3361 tattgcaccc gttctccgat tacgagtttc atttaaatca tgtgagcaaa aggccagcaa
     3421 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     3481 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     3541 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     3601 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     3661 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3721 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3781 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3841 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     3901 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3961 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     4021 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     4081 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     4141 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     4201 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     4261 ctatttcgtt catccatagt tgcatttaaa tttccgaact ctccaaggcc ctcgtcggaa
     4321 aatcttcaaa cctttcgtcc gatccatctt gcaggctacc tctcgaacga actatcgcaa
     4381 gtctcttggc cggccttgcg ccttggctat tgcttggcag cgcctatcgc caggtattac
     4441 tccaatcccg aatatccgag atcgggatca cccgagagaa gttcaaccta catcctcaat
     4501 cccgatctat ccgagatccg aggaatatcg aaatcggggc gcgcctggtg taccgagaac
     4561 gatcctctca gtgcgagtct cgacgatcca tatcgttgct tggcagtcag ccagtcggaa
     4621 tccagcttgg gacccaggaa gtccaatcgt cagatattgt actcaagcct ggtcacggca
     4681 gcgtaccgat ctgtttaaac ctagatattg atagtctgat cggtcaacgt ataatcgagt
     4741 cctagctttt gcaaacatct atcaagagac aggatcagca ggaggctttc gcatgagtat
     4801 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4861 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cgcgagtggg
     4921 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     4981 ctttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     5041 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     5101 ttcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     5161 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga ttggaggacc
     5221 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     5281 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     5341 aatggcaaca accttgcgta aactattaac tggcgaacta cttactctag cttcccggca
     5401 acagttgata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     5461 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     5521 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     5581 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     5641 taagcattgg taaccgattc taggtgcatt ggcgcagaaa aaaatgcctg atgcgacgct
     5701 gcgcgtctta tactcccaca tatgccagat tcagcaacgg atacggcttc cccaacttgc
     5761 ccacttccat acgtgtcctc cttaccagaa atttatcctt aagatcccga atcgtttaaa
     5821 ctcgactctg gctctatcga atctccgtcg tttcgagctt acgcgaacag ccgtggcgct
     5881 catttgctcg tcgggcatcg aatctcgtca gctatcgtca gcttaccttt ttggca
//
