LOCUS       pSF-CMV-EMCV-RLu        5742 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector for co-expressing a gene together with Renilla 
            luciferase expressed from the encephalomyocarditis virus IRES.
ACCESSION   .
VERSION     .
KEYWORDS    pSF-CMV-EMCV-RLuc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Oxford Genetics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5742)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5742
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..89
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     terminator      complement(94..141)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    complement(158..206)
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     enhancer        287..590
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        591..794
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    857..942
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     RBS             893..898
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     misc_signal     903..909
                     /label=Kozak sequence
                     /note="Kozak sequence"
     CDS             907..909
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     misc_feature    961..971
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     misc_feature    1011..1562
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             1565..2500
                     /label=RLuc
                     /note="Renilla luciferase"
     misc_feature    2506..2516
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    complement(2548..2669)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     terminator      2783..2830
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      2860..2996
                     /note="rrnG terminator"
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    3041..3112
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     rep_origin      complement(3338..3926)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     RBS             4659..4664
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             4672..5463
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      5479..5615
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 gcgatcgcgg ctcccgacat cttggaccat tagctccaca ggtatcttct tccctctagt
       61 ggtcataaca gcagcttcag ctacctctca attcaaaaaa cccctcaaga cccgtttaga
      121 ggccccaagg ggttatgcta tcaatcgttg cgttacacac acaaaaaacc aacacacatc
      181 catcttcgat ggatagcgat tttattatct aactgctgat cgagtgtagc cagatctagt
      241 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      301 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      361 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      421 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      481 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      541 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg ctgatgcggt
      601 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      661 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      721 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      781 atataagcag agctggttta gtgaaccgtc agatcagatc tttgtcgatc ctaccatcca
      841 ctcgacacac ccgccagcgg ccgctgccaa gcttccgagc tctcgaattc aaaggaggta
      901 cccaccatgg ggtaccgcga tatctacctc gaggtttcta gaagttgtct cctcctgcac
      961 tgactgactg atacaatcga tatgccaaaa gcaaagcgct atcgcgcctt acgttactgg
     1021 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     1081 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     1141 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     1201 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     1261 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     1321 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     1381 atggctcccc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     1441 tatgggatct gatctggggc ctcggtgcac atgcttttca tgtgtttagt cgaggttaaa
     1501 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     1561 taacatgacc agcaaagtgt atgatccgga acagcgcaaa cgcatgatta ccggcccgca
     1621 gtggtgggcg cgctgcaaac agatgaacgt gctggatagc tttattaact attatgatag
     1681 cgaaaaacat gcggaaaacg cggtgatttt tctgcatggc aacgcggcga gcagctatct
     1741 gtggcggcat gtggtgccgc atattgaacc agtggcgcgc tgcattattc cggatctgat
     1801 tggcatgggc aaaagcggca aaagcggcaa cggcagctat cgcctgctgg atcattataa
     1861 atatctgacc gcgtggtttg aactgctgaa cctgccgaaa aaaattattt ttgtgggcca
     1921 tgattggggc gcgtgcctgg cgtttcatta tagctatgaa catcaggata aaattaaagc
     1981 gattgtgcac gcggaaagcg tggtggatgt gattgaaagc tgggatgaat ggccggatat
     2041 tgaagaagat attgcgctga ttaaaagcga agaaggcgaa aaaatggtgc tggaaaacaa
     2101 cttttttgtg gaaaccatgc tgccgagcaa aattatgcgc aaactggaac cggaagaatt
     2161 tgcggcgtat ctggaaccgt ttaaagaaaa aggcgaagtg cgccgcccga ccctgagctg
     2221 gccgcgcgaa attccgctgg tgaaaggcgg caaaccggat gtggtccaga ttgtgcgcaa
     2281 ctataacgcg tatctgcgcg cgagcgatga tctgccgaaa atgtttattg aaagcgatcc
     2341 gggctttttt agcaacgcga ttgtggaagg cgcgaaaaaa tttccgaaca ccgaatttgt
     2401 gaaagtgaag ggcctgcatt ttagccagga agatgcgccg gatgaaatgg gcaaatatat
     2461 taagagcttt gtggaacgcg tgctgaaaaa cgaacagggc tagcttgact gactgagata
     2521 cagcgtacct tcagctcaca gacatgataa gatacattga tgagtttgga caaaccacaa
     2581 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     2641 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     2701 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     2761 ttggcccatc tctatcggta tcgtagcata accccttggg gcctctaaac gggtcttgag
     2821 gggttttttg tgcccctcgg gccggattgc tatctaccgg cattggcgca gaaaaaaatg
     2881 cctgatgcga cgctgcgcgt cttatactcc cacatatgcc agattcagca acggatacgg
     2941 cttccccaac ttgcccactt ccatacgtgt cctccttacc agaaatttat ccttaaggtc
     3001 gtcagctatc ctgcaggcga tctctcgatt tcgatcaaga cattccttta atggtctttt
     3061 ctggacacca ctaggggtca gaagtagttc atcaaacttt cttccctccc taatctcatt
     3121 ggttaccttg ggctatcgaa acttaattaa ccagtcaagt cagctacttg gcgagatcga
     3181 cttgtctggg tttcgactac gctcagaatt gcgtcagtca agttcgatct ggtccttgct
     3241 attgcacccg ttctccgatt acgagtttca tttaaatcat gtgagcaaaa ggccagcaaa
     3301 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     3361 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     3421 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     3481 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     3541 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     3601 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     3661 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     3721 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     3781 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     3841 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     3901 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     3961 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     4021 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     4081 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     4141 tatttcgttc atccatagtt gcatttaaat ttccgaactc tccaaggccc tcgtcggaaa
     4201 atcttcaaac ctttcgtccg atccatcttg caggctacct ctcgaacgaa ctatcgcaag
     4261 tctcttggcc ggccttgcgc cttggctatt gcttggcagc gcctatcgcc aggtattact
     4321 ccaatcccga atatccgaga tcgggatcac ccgagagaag ttcaacctac atcctcaatc
     4381 ccgatctatc cgagatccga ggaatatcga aatcggggcg cgcctggtgt accgagaacg
     4441 atcctctcag tgcgagtctc gacgatccat atcgttgctt ggcagtcagc cagtcggaat
     4501 ccagcttggg acccaggaag tccaatcgtc agatattgta ctcaagcctg gtcacggcag
     4561 cgtaccgatc tgtttaaacc tagatattga tagtctgatc ggtcaacgta taatcgagtc
     4621 ctagcttttg caaacatcta tcaagagaca ggatcagcag gaggctttcg catgattgaa
     4681 caagatggat tgcacgcagg ttctccggcg gcttgggtgg agaggctatt cggctatgac
     4741 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     4801 cgtccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
     4861 gcagcgcggc tatcgtggct ggcgacgacg ggcgttcctt gcgcggctgt gctcgacgtt
     4921 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     4981 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     5041 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     5101 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     5161 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgt ctatgcccga cggcgaggat
     5221 ctcgtcgtga cccacggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     5281 tctggattca tcgactgtgg ccgtctgggt gtggcggacc gctatcagga catagcgttg
     5341 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt ccttgtgctt
     5401 tacggtatcg ccgcgcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     5461 ttctgaccga ttctaggtgc attggcgcag aaaaaaatgc ctgatgcgac gctgcgcgtc
     5521 ttatactccc acatatgcca gattcagcaa cggatacggc ttccccaact tgcccacttc
     5581 catacgtgtc ctccttacca gaaatttatc cttaaggtcg tttaaactcg actctggctc
     5641 tatcgaatct ccgtcgtttc gagcttacgc gaacagccgt ggcgctcatt tgctcgtcgg
     5701 gcatcgaatc tcgtcagcta tcgtcagctt acctttttgg ca
//
