LOCUS       pSF-CMV-EMCV-FLu        6461 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector for co-expressing a gene together with firefly 
            luciferase expressed from the encephalomyocarditis virus IRES.
ACCESSION   .
VERSION     .
KEYWORDS    pSF-CMV-EMCV-FLuc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Oxford Genetics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6461)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..89
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     terminator      complement(94..141)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    complement(158..206)
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     enhancer        287..590
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        591..794
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    857..942
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     RBS             893..898
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     misc_signal     903..909
                     /label=Kozak sequence
                     /note="Kozak sequence"
     CDS             907..909
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     misc_feature    961..971
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     misc_feature    1011..1562
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             1565..3214
                     /label=luciferase
                     /note="firefly luciferase"
     misc_feature    3225..3235
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    complement(3267..3388)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     terminator      3502..3549
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      3579..3715
                     /note="rrnG terminator"
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    3760..3831
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     rep_origin      complement(4057..4645)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     RBS             5378..5383
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             5391..6182
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      6198..6334
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 gcgatcgcgg ctcccgacat cttggaccat tagctccaca ggtatcttct tccctctagt
       61 ggtcataaca gcagcttcag ctacctctca attcaaaaaa cccctcaaga cccgtttaga
      121 ggccccaagg ggttatgcta tcaatcgttg cgttacacac acaaaaaacc aacacacatc
      181 catcttcgat ggatagcgat tttattatct aactgctgat cgagtgtagc cagatctagt
      241 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      301 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      361 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      421 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      481 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      541 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg ctgatgcggt
      601 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      661 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      721 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      781 atataagcag agctggttta gtgaaccgtc agatcagatc tttgtcgatc ctaccatcca
      841 ctcgacacac ccgccagcgg ccgctgccaa gcttccgagc tctcgaattc aaaggaggta
      901 cccaccatgg ggtaccgcga tatctacctc gaggtttcta gaagttgtct cctcctgcac
      961 tgactgactg atacaatcga tatgccaaaa gcaaagcgct atcgcgcctt acgttactgg
     1021 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     1081 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     1141 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     1201 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     1261 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     1321 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     1381 atggctcccc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     1441 tatgggatct gatctggggc ctcggtgcac atgcttttca tgtgtttagt cgaggttaaa
     1501 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     1561 taacatggaa gatgccaaaa acattaagaa aggcccagcg ccattctacc cactcgaaga
     1621 cgggaccgct ggcgagcagc tgcataaagc catgaagcgc tacgccctgg tgcccggcac
     1681 catcgccttt accgacgcac atatcgaggt ggacattacc tacgccgagt acttcgagat
     1741 gagcgttcgg ctggcagaag ctatgaagcg ctatgggctg aatacaaacc atcggatcgt
     1801 ggtgtgtagc gagaatagct tgcagttctt catgcccgtg ttgggtgccc tgttcatcgg
     1861 tgtggctgtg gccccagcta acgacatcta caacgagcgc gagctgctga acagcatggg
     1921 catcagccag cccaccgtcg tattcgtgag caagaaaggg ctgcaaaaga tcctcaacgt
     1981 gcaaaagaag ctaccgatca tacaaaagat catcatcatg gatagcaaga ccgactacca
     2041 gggcttccaa agcatgtaca ccttcgtgac ttcccatttg ccacccggct tcaacgagta
     2101 cgacttcgtg cccgagagct tcgaccggga caaaaccatc gccctgatca tgaacagtag
     2161 tggcagtacc ggattgccca agggcgtagc cctaccgcac cgcaccgctt gtgtccgatt
     2221 cagtcatgcc cgcgacccca tcttcggcaa ccagatcatc cccgacaccg ctatcctgag
     2281 cgtggtgcca tttcaccacg gcttcggcat gttcaccacg ctgggctact tgatctgcgg
     2341 ctttcgggtc gtgctcatgt accgcttcga ggaagagcta ttcttgcgca gcttgcaaga
     2401 ctataagatt caatctgccc tgctggtgcc cacactattt agcttcttcg ctaagagcac
     2461 tctcatcgac aagtacgacc taagcaactt gcacgagatc gccagcggcg gggcgccgct
     2521 cagcaaggag gtaggtgagg ccgtggccaa acgcttccac ctaccaggca tccgccaggg
     2581 ctacggcctg acagaaacaa ccagcgccat tctgatcacc cccgaagggg acgacaagcc
     2641 tggcgcagta ggcaaggtgg tgcccttctt cgaggctaag gtggtggact tggacaccgg
     2701 taagacactg ggtgtgaacc agcgcggcga gctgtgcgtc cgtggcccca tgatcatgag
     2761 cggctacgtt aacaaccccg aggctacaaa cgctctcatc gacaaggacg gctggctgca
     2821 tagcggcgac atcgcctact gggacgagga cgagcacttc ttcatcgtgg accggctgaa
     2881 gagcctgatc aaatacaagg gctaccaggt agccccagcc gaactggaga gcatcctgct
     2941 gcaacacccc aacatcttcg acgccggggt cgccggcctg cccgacgacg atgccggcga
     3001 gctgcccgcc gcagtcgtcg tgctggaaca cggtaaaacc atgaccgaga aggagatcgt
     3061 ggactatgtg gccagccagg ttacaaccgc caagaagctg cgcggtggtg ttgtgttcgt
     3121 ggacgaggtg cctaaaggac tgaccggcaa gttggacgcc cgcaagatcc gcgagattct
     3181 cattaaggcc aagaagggcg gcaagatcgc cgtctaggct agcttgactg actgagatac
     3241 agcgtacctt cagctcacag acatgataag atacattgat gagtttggac aaaccacaac
     3301 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     3361 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     3421 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
     3481 tggcccatct ctatcggtat cgtagcataa ccccttgggg cctctaaacg ggtcttgagg
     3541 ggttttttgt gcccctcggg ccggattgct atctaccggc attggcgcag aaaaaaatgc
     3601 ctgatgcgac gctgcgcgtc ttatactccc acatatgcca gattcagcaa cggatacggc
     3661 ttccccaact tgcccacttc catacgtgtc ctccttacca gaaatttatc cttaaggtcg
     3721 tcagctatcc tgcaggcgat ctctcgattt cgatcaagac attcctttaa tggtcttttc
     3781 tggacaccac taggggtcag aagtagttca tcaaactttc ttccctccct aatctcattg
     3841 gttaccttgg gctatcgaaa cttaattaac cagtcaagtc agctacttgg cgagatcgac
     3901 ttgtctgggt ttcgactacg ctcagaattg cgtcagtcaa gttcgatctg gtccttgcta
     3961 ttgcacccgt tctccgatta cgagtttcat ttaaatcatg tgagcaaaag gccagcaaaa
     4021 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     4081 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     4141 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     4201 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     4261 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     4321 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     4381 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4441 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     4501 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4561 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4621 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4681 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4741 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4801 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     4861 atttcgttca tccatagttg catttaaatt tccgaactct ccaaggccct cgtcggaaaa
     4921 tcttcaaacc tttcgtccga tccatcttgc aggctacctc tcgaacgaac tatcgcaagt
     4981 ctcttggccg gccttgcgcc ttggctattg cttggcagcg cctatcgcca ggtattactc
     5041 caatcccgaa tatccgagat cgggatcacc cgagagaagt tcaacctaca tcctcaatcc
     5101 cgatctatcc gagatccgag gaatatcgaa atcggggcgc gcctggtgta ccgagaacga
     5161 tcctctcagt gcgagtctcg acgatccata tcgttgcttg gcagtcagcc agtcggaatc
     5221 cagcttggga cccaggaagt ccaatcgtca gatattgtac tcaagcctgg tcacggcagc
     5281 gtaccgatct gtttaaacct agatattgat agtctgatcg gtcaacgtat aatcgagtcc
     5341 tagcttttgc aaacatctat caagagacag gatcagcagg aggctttcgc atgattgaac
     5401 aagatggatt gcacgcaggt tctccggcgg cttgggtgga gaggctattc ggctatgact
     5461 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     5521 gtccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg
     5581 cagcgcggct atcgtggctg gcgacgacgg gcgttccttg cgcggctgtg ctcgacgttg
     5641 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     5701 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     5761 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     5821 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     5881 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgtc tatgcccgac ggcgaggatc
     5941 tcgtcgtgac ccacggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     6001 ctggattcat cgactgtggc cgtctgggtg tggcggaccg ctatcaggac atagcgttgg
     6061 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc cttgtgcttt
     6121 acggtatcgc cgcgcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     6181 tctgaccgat tctaggtgca ttggcgcaga aaaaaatgcc tgatgcgacg ctgcgcgtct
     6241 tatactccca catatgccag attcagcaac ggatacggct tccccaactt gcccacttcc
     6301 atacgtgtcc tccttaccag aaatttatcc ttaaggtcgt ttaaactcga ctctggctct
     6361 atcgaatctc cgtcgtttcg agcttacgcg aacagccgtg gcgctcattt gctcgtcggg
     6421 catcgaatct cgtcagctat cgtcagctta cctttttggc a
//
