LOCUS       62056_19565        4362 bp DNA     circular SYN 27-FEB-2021
DEFINITION  Cloning vector pSEVAb447, complete sequence.
ACCESSION   MW476674
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Damalas SG, Batianis C, Martin-Pascual M, de Lorenzo V, Martins Dos 
            Santos VAP.
  TITLE     SEVA 3.1: enabling interoperability of DNA assembly among the SEVA, 
            BioBricks and Type IIS restriction enzyme standards
  JOURNAL   Microb Biotechnol 13 (6), 1793-1806 (2020)
  PUBMED    32710525
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   Damalas SG, Batianis C, Martin Pascual M, de Lorenzo V, Martins dos 
            Santos VAP.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JAN-2021) Laboratory of Systems and Synthetic Biology,
            Wageningen University, Stippeneng 4, Wageningen, Gelderland 6708 WE,
            The Netherlands
REFERENCE   3  (bases 1 to 4362)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb 
            Biotechnol"; date: "2020"; volume: "13"; issue: "6"; pages: 
            "1793-1806"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-JAN-2021) Laboratory of Systems and Synthetic Biology, 
            Wageningen University, Stippeneng 4, Wageningen, Gelderland 6708 WE,
            The Netherlands"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     regulatory      23..57
                     /label=promoter BBa_J23105
                     /note="promoter BBa_J23105"
                     /regulatory_class="promoter"
     regulatory      66..77
                     /label=RBS.20
                     /note="RBS.20"
                     /regulatory_class="ribosome_binding_site"
     CDS             84..797
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pedelacq et al., 2006)"
     misc_feature    804..824
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      855..949
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1185..1973
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     oriT            2122..2230
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      2324..2912
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2969..3799)
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     rep_origin      3813..4164
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      complement(4237..4323)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gaattcgcgg ccgcttctag agtttacggc tagctcagtc ctaggtacta tgctagctac
       61 tagagtattt aaggctgtac tagatgcgta aaggcgaaga actgttcacg ggcgtagttc
      121 cgattctggt cgagctggac ggcgatgtga acggtcataa gtttagcgtt cgcggtgaag
      181 gtgagggcga cgcgaccaac ggcaaactga ccctgaagtt catctgcacc accggtaaac
      241 tgccggtgcc ttggccgacc ttggtgacga cgttgacgta tggcgtgcag tgttttgcgc
      301 gttatccgga ccacatgaaa caacacgatt tcttcaaatc tgcgatgccg gagggttacg
      361 tccaggagcg taccatttcc ttcaaggatg atggctacta caaaactcgc gcagaggtta
      421 agtttgaagg tgacacgctg gtcaatcgta tcgaattgaa gggtatcgac tttaaagagg
      481 atggtaacat tctgggccat aaactggagt ataacttcaa cagccataat gtttacatta
      541 cggcagacaa gcaaaagaac ggcatcaagg ccaatttcaa gattcgccac aatgttgagg
      601 acggtagcgt ccaactggcc gaccattacc agcagaacac cccaattggt gacggtccgg
      661 ttttgctgcc ggataatcac tatctgagca cccaaagcgt gctgagcaaa gatccgaacg
      721 aaaaacgtga tcacatggtc ctgctggaat ttgtgaccgc tgcgggcatc acccacggta
      781 tggatgaact gtacaaataa taatactagt agcggccgct gcagtccggc aaaaaagggc
      841 aaggtctagt cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga
      901 tttgttcaga acgctcggtt gccgccgggc gttttttatt ggtgagaatc caggggtccc
      961 caataattac gatttacgta tttaaatgaa ccttgaccga acgcagcggt ggtaacggcg
     1021 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     1081 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     1141 atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgagg gaagcggtga
     1201 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     1261 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     1321 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     1381 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     1441 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     1501 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     1561 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     1621 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     1681 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     1741 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     1801 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     1861 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     1921 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataagaca
     1981 attgtccttt tccgctgcat aaccctgctt cggggtcatt atagcgattt tttcggtata
     2041 tccatccttt ttcgcacgat atacaggatt ttgccaaagg gttcgtgtag actttccttg
     2101 gtgtatccaa cggcgtcagc cgggcaggat aggtgaagta ggcccacccg cgagcgggtg
     2161 ttccttcttc actgtccctt attcgcacct ggcggtgctc aacgggaatc ctgctctgcg
     2221 aggctggccg taggccggcc gataatctca tgaccaaaat cccttaacgt gagttttcgt
     2281 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     2341 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     2401 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     2461 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     2521 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     2581 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     2641 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     2701 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggc
     2761 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     2821 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     2881 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc gtgaaaggca
     2941 ggccggtccg tggtggccac ggcctctagg ccagatccag cggcatctgg gttagtcgag
     3001 cgcgggccgc ttcccatgtc tcaccagggc gagcctgttt cgcgatctca gcatctgaaa
     3061 tcttcccggc cttgcgcttc gctggggcct tacccaccgc cttggcgggc ttcttcggtc
     3121 caaaactgaa caacagatgt gtgaccttgc gcccggtctt tcgctgcgcc cactccacct
     3181 gtagcgggct gtgctcgttg atctgcgtca cggctggatc aagcactcgc aacttgaagt
     3241 ccttgatcga gggataccgg ccttccagtt gaaaccactt tcgcagctgg tcaatttcta
     3301 tttcgcgctg gccgatgctg tcccattgca tgagcagctc gtaaagcctg atcgcgtggg
     3361 tgctgtccat cttggccacg tcagccaagg cgtatttggt gaactgtttg gtgagttccg
     3421 tcaggtacgg cagcatgtct ttggtgaacc tgagttctac acggccctca ccctcccggt
     3481 agatgattgt ttgcacccag ccggtaatca tcacactcgg tcttttcccc ttgccattgg
     3541 gctcttgggt taaccggact tcccgccgtt tcaggcgcag ggccgcttct ttgagctggt
     3601 tgtaggaaga ttcgataggg acacccgcca tcgtcgctat gtcctccgcc gtcactgaat
     3661 acatcacttc atcggtgaca ggctcgctcc tcttcacctg gctaatacag gccagaacga
     3721 tccgctgttc ctgaacactg aggcgatacg cggcctcgac cagggcattg cttttgtaaa
     3781 ccattggggg tgaggccacg ttcgacattc cttgtgtata aggggacact gtatctgcgt
     3841 cccacaatac aacaaatccg tccctttaca acaacaaatc cgtcccttct taacaacaaa
     3901 tccgtccctt aatggcaaca aatccgtccc tttttaaact ctacaggcca cggattacgt
     3961 ggcctgtaga cgtcctaaaa ggtttaaaag ggaaaaggaa gaaaagggtg gaaacgcaaa
     4021 aaacgcacca ctacgtggcc ccgttggggc cgcatttgtg cccctgaagg ggcgggggag
     4081 gcgtctgggc aatccccgtt ttaccagtcc cctatcgccg cctgagaggg cgcaggaagc
     4141 gagtaatcag ggtatcgagg cggattcacc cttggcgtcc aaccagcggc accagcggcg
     4201 cctgagaggg gcgcgcccag ctgtctaggg cggcggattt gtcctactca ggagagcgtt
     4261 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
     4321 ttgatgcctt taattaatag ctttcgctaa ggatgatttc tg
//
