LOCUS       62056_19540        3856 bp DNA     circular SYN 27-FEB-2021
DEFINITION  Cloning vector pSEVAb237, complete sequence.
ACCESSION   MW476670
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Damalas SG, Batianis C, Martin-Pascual M, de Lorenzo V, Martins Dos 
            Santos VAP.
  TITLE     SEVA 3.1: enabling interoperability of DNA assembly among the SEVA, 
            BioBricks and Type IIS restriction enzyme standards
  JOURNAL   Microb Biotechnol 13 (6), 1793-1806 (2020)
  PUBMED    32710525
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Damalas SG, Batianis C, Martin Pascual M, de Lorenzo V, Martins dos 
            Santos VAP.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JAN-2021) Laboratory of Systems and Synthetic Biology,
            Wageningen University, Stippeneng 4, Wageningen, Gelderland 6708 WE,
            The Netherlands
REFERENCE   3  (bases 1 to 3856)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb 
            Biotechnol"; date: "2020"; volume: "13"; issue: "6"; pages: 
            "1793-1806"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-JAN-2021) Laboratory of Systems and Synthetic Biology, 
            Wageningen University, Stippeneng 4, Wageningen, Gelderland 6708 WE,
            The Netherlands"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     regulatory      23..57
                     /label=promoter BBa_J23105
                     /note="promoter BBa_J23105"
                     /regulatory_class="promoter"
     regulatory      66..77
                     /label=RBS.20
                     /note="RBS.20"
                     /regulatory_class="ribosome_binding_site"
     CDS             84..797
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pedelacq et al., 2006)"
     misc_feature    804..824
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      855..949
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1081..1893
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     oriT            2063..2171
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(2185..2844)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(2845..3616)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     terminator      complement(3731..3817)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gaattcgcgg ccgcttctag agtttacggc tagctcagtc ctaggtacta tgctagctac
       61 tagagtattt aaggctgtac tagatgcgta aaggcgaaga actgttcacg ggcgtagttc
      121 cgattctggt cgagctggac ggcgatgtga acggtcataa gtttagcgtt cgcggtgaag
      181 gtgagggcga cgcgaccaac ggcaaactga ccctgaagtt catctgcacc accggtaaac
      241 tgccggtgcc ttggccgacc ttggtgacga cgttgacgta tggcgtgcag tgttttgcgc
      301 gttatccgga ccacatgaaa caacacgatt tcttcaaatc tgcgatgccg gagggttacg
      361 tccaggagcg taccatttcc ttcaaggatg atggctacta caaaactcgc gcagaggtta
      421 agtttgaagg tgacacgctg gtcaatcgta tcgaattgaa gggtatcgac tttaaagagg
      481 atggtaacat tctgggccat aaactggagt ataacttcaa cagccataat gtttacatta
      541 cggcagacaa gcaaaagaac ggcatcaagg ccaatttcaa gattcgccac aatgttgagg
      601 acggtagcgt ccaactggcc gaccattacc agcagaacac cccaattggt gacggtccgg
      661 ttttgctgcc ggataatcac tatctgagca cccaaagcgt gctgagcaaa gatccgaacg
      721 aaaaacgtga tcacatggtc ctgctggaat ttgtgaccgc tgcgggcatc acccacggta
      781 tggatgaact gtacaaataa taatactagt agcggccgct gcagtccggc aaaaaaggtc
      841 aaggtctagt cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga
      901 tttgttcaga acgctcggtt gccgccgggc gttttttatt ggtgagaatc caggggtccc
      961 caataattac gatttaaatt tgtgtctcaa aatctctgat gttacattgc acaagataaa
     1021 aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
     1081 atgagccata ttcagcgtga aacgagctgt agccgtccgc gtctgaacag caacatggat
     1141 gcggatctgt atggctataa atgggcgcgt gataacgtgg gtcagagcgg cgcgaccatt
     1201 tatcgtctgt atggcaaacc ggatgcgccg gaactgtttc tgaaacatgg caaaggcagc
     1261 gtggcgaacg atgtgaccga tgaaatggtg cgtctgaact ggctgaccga atttatgccg
     1321 ctgccgacca ttaaacattt tattcgcacc ccggatgatg cgtggctgct gaccaccgcg
     1381 attccgggca aaaccgcgtt tcaggtgctg gaagaatatc cggatagcgg cgaaaacatt
     1441 gtggatgcgc tggccgtgtt tctgcgtcgt ctgcatagca ttccggtgtg caactgcccg
     1501 tttaacagcg atcgtgtgtt tcgtctggcc caggcgcaga gccgtatgaa caacggcctg
     1561 gtggatgcga gcgattttga tgatgaacgt aacggctggc cggtggaaca ggtgtggaaa
     1621 gaaatgcata aactgctgcc gtttagcccg gatagcgtgg tgacccacgg cgattttagc
     1681 ctggataacc tgattttcga tgaaggcaaa ctgattggct gcattgatgt gggccgtgtg
     1741 ggcattgcgg atcgttatca ggatctggcc attctgtgga actgcctggg cgaatttagc
     1801 ccgagcctgc aaaaacgtct gtttcagaaa tatggcattg ataatccgga tatgaacaaa
     1861 ctgcaatttc atctgatgct ggatgaattt ttctaataat taattggacc gcggtccgcg
     1921 cgttgtcctt ttccgctgca taaccctgct tcggggtcat tatagcgatt ttttcggtat
     1981 atccatcctt tttcgcacga tatacaggat tttgccaaag ggttcgtgta gactttcctt
     2041 ggtgtatcca acggcgtcag ccgggcagga taggtgaagt aggcccaccc gcgagcgggt
     2101 gttccttctt cactgtccct tattcgcacc tggcggtgct caacgggaat cctgctctgc
     2161 gaggctggcc gtaggccggc cctaccggcg cggcagcgtt acccgtgtcg gcggctccaa
     2221 cggctcgcca tcgtccagaa aacacggctc atcgggcatc ggcaggcgct gctgcccgcg
     2281 ccgttcccat tcctccgttt cggtcaaggc tggcaggtct ggttccatgc ccggaatgcc
     2341 gggctggctg ggcggctcct cgccggggcc ggtcggtagt tgctgctcgc ccggatacag
     2401 ggtcgggatg cggcgcaggt cgccatgccc caacagcgat tcgtcctggt cgtcgtgatc
     2461 aaccaccacg gcggcactga acaccgacag gcgcaactgg tcgcggggct ggccccacgc
     2521 cacgcggtca ttgaccacgt aggccgacac ggtgccgggg ccgttgagct tcacgacgga
     2581 gatccagcgc tcggccacca agtccttgac tgcgtattgg accgtccgca aagaacgtcc
     2641 gatgagcttg gaaagtgtct tctggctgac caccacggcg ttctggtggc ccatctgcgc
     2701 cacgaggtga tgcagcagca ttgccgccgt gggtttcctc gcaataagcc cggcccacgc
     2761 ctcatgcgct ttgcgttccg tttgcaccca gtgaccgggc ttgttcttgg cttgaatgcc
     2821 gatttctctg gactgcgtgg ccatgcttat ctccatgcgg taggggtgcc gcacggttgc
     2881 ggcaccatgc gcaatcagct gcaacttttc ggcagcgcga caacaattat gcgttgcgta
     2941 aaagtggcag tcaattacag attttcttta acctacgcaa tgagctattg cggggggtgc
     3001 cgcaatgagc tgttgcgtac cccccttttt taagttgttg atttttaagt ctttcgcatt
     3061 tcgccctata tctagttctt tggtgcccaa agaagggcac ccctgcgggg ttcccccacg
     3121 ccttcggcgc ggctccccct ccggcaaaaa gtggcccctc cggggcttgt tgatcgactg
     3181 cgcggccttc ggccttgccc aaggtggcgc tgcccccttg gaacccccgc actcgccgcc
     3241 gtgaggctcg gggggcaggc gggcgggctt cgcccttcga ctgcccccac tcgcataggc
     3301 ttgggtcgtt ccaggcgcgt caaggccaag ccgctgcgcg gtcgctgcgc gagccttgac
     3361 ccgccttcca cttggtgtcc aaccggcaag cgaagcgcgc aggccgcagg ccggaggctt
     3421 ttccccagag aaaattaaaa aaattgatgg ggcaaggccg caggccgcgc agttggagcc
     3481 ggtgggtatg tggtcgaagg ctgggtagcc ggtgggcaat ccctgtggtc aagctcgtgg
     3541 gcaggcgcag cctgtccatc agcttgtcca gcagggttgt ccacgggccg agcgaagcga
     3601 gccagccggt ggccgctcgc ggccatcgtc cacatatcca cgggctggca agggagcgca
     3661 gcgaccgcgc agggcgaagc ccggagagca agcccgtagg gggggcgcgc ccagctgtct
     3721 agggcggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
     3781 aggcccagtc tttcgactga gcctttcgtt ttatttgatg cctttaatta atagctttcg
     3841 ctaaggatga tttctg
//
