LOCUS       62056_19525        4404 bp DNA     circular SYN 27-FEB-2021
DEFINITION  Cloning vector pSEVAb147, complete sequence.
ACCESSION   MW476667
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Damalas SG, Batianis C, Martin-Pascual M, de Lorenzo V, Martins Dos 
            Santos VAP.
  TITLE     SEVA 3.1: enabling interoperability of DNA assembly among the SEVA, 
            BioBricks and Type IIS restriction enzyme standards
  JOURNAL   Microb Biotechnol 13 (6), 1793-1806 (2020)
  PUBMED    32710525
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Damalas SG, Batianis C, Martin Pascual M, de Lorenzo V, Martins dos 
            Santos VAP.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JAN-2021) Laboratory of Systems and Synthetic Biology,
            Wageningen University, Stippeneng 4, Wageningen, Gelderland 6708 WE,
            The Netherlands
REFERENCE   3  (bases 1 to 4404)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb 
            Biotechnol"; date: "2020"; volume: "13"; issue: "6"; pages: 
            "1793-1806"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-JAN-2021) Laboratory of Systems and Synthetic Biology, 
            Wageningen University, Stippeneng 4, Wageningen, Gelderland 6708 WE,
            The Netherlands"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     regulatory      23..57
                     /label=promoter BBa_J23105
                     /note="promoter BBa_J23105"
                     /regulatory_class="promoter"
     regulatory      66..77
                     /label=RBS.20
                     /note="RBS.20"
                     /regulatory_class="ribosome_binding_site"
     CDS             84..797
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pedelacq et al., 2006)"
     misc_feature    804..824
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      855..949
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1022..1113
                     /label=AmpR promoter
     CDS             1114..1971
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      1978..2017
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2164..2272
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      2366..2954
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3011..3841)
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     rep_origin      3855..4206
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      complement(4279..4365)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gaattcgcgg ccgcttctag agtttacggc tagctcagtc ctaggtacta tgctagctac
       61 tagagtattt aaggctgtac tagatgcgta aaggcgaaga actgttcacg ggcgtagttc
      121 cgattctggt cgagctggac ggcgatgtga acggtcataa gtttagcgtt cgcggtgaag
      181 gtgagggcga cgcgaccaac ggcaaactga ccctgaagtt catctgcacc accggtaaac
      241 tgccggtgcc ttggccgacc ttggtgacga cgttgacgta tggcgtgcag tgttttgcgc
      301 gttatccgga ccacatgaaa caacacgatt tcttcaaatc tgcgatgccg gagggttacg
      361 tccaggagcg taccatttcc ttcaaggatg atggctacta caaaactcgc gcagaggtta
      421 agtttgaagg tgacacgctg gtcaatcgta tcgaattgaa gggtatcgac tttaaagagg
      481 atggtaacat tctgggccat aaactggagt ataacttcaa cagccataat gtttacatta
      541 cggcagacaa gcaaaagaac ggcatcaagg ccaatttcaa gattcgccac aatgttgagg
      601 acggtagcgt ccaactggcc gaccattacc agcagaacac cccaattggt gacggtccgg
      661 ttttgctgcc ggataatcac tatctgagca cccaaagcgt gctgagcaaa gatccgaacg
      721 aaaaacgtga tcacatggtc ctgctggaat ttgtgaccgc tgcgggcatc acccacggta
      781 tggatgaact gtacaaataa taatactagt agcggccgct gcagtccggc aaaaaagggc
      841 aaggtctagt cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga
      901 tttgttcaga acgctcggtt gccgccgggc gttttttatt ggtgagaatc caggggtccc
      961 caataattac gatttaaatt agtagcccgc ctaatgagcg ggcttttttt taattcccct
     1021 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     1081 taaatgcttc aataatattg aaaaaggaag agtatgagca ttcagcattt tcgtgtggcg
     1141 ctgattccgt tttttgcggc gttttgcctg ccggtgtttg cgcatccgga aaccctggtg
     1201 aaagtgaaag atgcggaaga tcaactgggt gcgcgcgtgg gctatattga actggatctg
     1261 aacagcggca aaattctgga atcttttcgt ccggaagaac gttttccgat gatgagcacc
     1321 tttaaagtgc tgctgtgcgg tgcggttctg agccgtgtgg atgcgggcca ggaacaactg
     1381 ggccgtcgta ttcattatag ccagaacgat ctggtggaat atagcccggt gaccgaaaaa
     1441 catctgaccg atggcatgac cgtgcgtgaa ctgtgcagcg cggcgattac catgagcgat
     1501 aacaccgcgg cgaacctgct gctgacgacc attggcggtc cgaaagaact gaccgcgttt
     1561 ctgcataaca tgggcgatca tgtgacccgt ctggatcgtt gggaaccgga actgaacgaa
     1621 gcgattccga acgatgaacg tgataccacc atgccggcag caatggcgac caccctgcgt
     1681 aaactgctga cgggtgagct gctgaccctg gcaagccgcc agcaactgat tgattggatg
     1741 gaagcggata aagtggcggg tccgctgctg cgtagcgcgc tgccggctgg ctggtttatt
     1801 gcggataaaa gcggtgcggg cgaacgtggc agccgtggca ttattgcggc gctgggcccg
     1861 gatggtaaac cgagccgtat tgtggtgatt tataccaccg gcagccaggc gacgatggat
     1921 gaacgtaacc gtcagattgc ggaaattggc gcgagcctga ttaaacattg gtaaaccgat
     1981 acaattaaag gctccttttg gagccttttt ttttggacga cccttgtcct tttccgctgc
     2041 ataaccctgc ttcggggtca ttatagcgat tttttcggta tatccatcct ttttcgcacg
     2101 atatacagga ttttgccaaa gggttcgtgt agactttcct tggtgtatcc aacggcgtca
     2161 gccgggcagg ataggtgaag taggcccacc cgcgagcggg tgttccttct tcactgtccc
     2221 ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc cgtaggccgg
     2281 ccgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     2341 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2401 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2461 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     2521 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2581 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2641 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2701 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2761 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gcatccggta agcggcaggg
     2821 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2881 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     2941 ggagcctatg gaaaaacgcc agcaacgcgg ccgtgaaagg caggccggtc cgtggtggcc
     3001 acggcctcta ggccagatcc agcggcatct gggttagtcg agcgcgggcc gcttcccatg
     3061 tctcaccagg gcgagcctgt ttcgcgatct cagcatctga aatcttcccg gccttgcgct
     3121 tcgctggggc cttacccacc gccttggcgg gcttcttcgg tccaaaactg aacaacagat
     3181 gtgtgacctt gcgcccggtc tttcgctgcg cccactccac ctgtagcggg ctgtgctcgt
     3241 tgatctgcgt cacggctgga tcaagcactc gcaacttgaa gtccttgatc gagggatacc
     3301 ggccttccag ttgaaaccac tttcgcagct ggtcaatttc tatttcgcgc tggccgatgc
     3361 tgtcccattg catgagcagc tcgtaaagcc tgatcgcgtg ggtgctgtcc atcttggcca
     3421 cgtcagccaa ggcgtatttg gtgaactgtt tggtgagttc cgtcaggtac ggcagcatgt
     3481 ctttggtgaa cctgagttct acacggccct caccctcccg gtagatgatt gtttgcaccc
     3541 agccggtaat catcacactc ggtcttttcc ccttgccatt gggctcttgg gttaaccgga
     3601 cttcccgccg tttcaggcgc agggccgctt ctttgagctg gttgtaggaa gattcgatag
     3661 ggacacccgc catcgtcgct atgtcctccg ccgtcactga atacatcact tcatcggtga
     3721 caggctcgct cctcttcacc tggctaatac aggccagaac gatccgctgt tcctgaacac
     3781 tgaggcgata cgcggcctcg accagggcat tgcttttgta aaccattggg ggtgaggcca
     3841 cgttcgacat tccttgtgta taaggggaca ctgtatctgc gtcccacaat acaacaaatc
     3901 cgtcccttta caacaacaaa tccgtccctt cttaacaaca aatccgtccc ttaatggcaa
     3961 caaatccgtc cctttttaaa ctctacaggc cacggattac gtggcctgta gacgtcctaa
     4021 aaggtttaaa agggaaaagg aagaaaaggg tggaaacgca aaaaacgcac cactacgtgg
     4081 ccccgttggg gccgcatttg tgcccctgaa ggggcggggg aggcgtctgg gcaatccccg
     4141 ttttaccagt cccctatcgc cgcctgagag ggcgcaggaa gcgagtaatc agggtatcga
     4201 ggcggattca cccttggcgt ccaaccagcg gcaccagcgg cgcctgagag gggcgcgccc
     4261 agctgtctag ggcggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     4321 aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tttaattaat
     4381 agctttcgct aaggatgatt tctg
//
