LOCUS       62056_19520        3957 bp DNA     circular SYN 27-FEB-2021
DEFINITION  Cloning vector pSEVAb137, complete sequence.
ACCESSION   MW476666
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Damalas SG, Batianis C, Martin-Pascual M, de Lorenzo V, Martins Dos 
            Santos VAP.
  TITLE     SEVA 3.1: enabling interoperability of DNA assembly among the SEVA, 
            BioBricks and Type IIS restriction enzyme standards
  JOURNAL   Microb Biotechnol 13 (6), 1793-1806 (2020)
  PUBMED    32710525
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Damalas SG, Batianis C, Martin Pascual M, de Lorenzo V, Martins dos 
            Santos VAP.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JAN-2021) Laboratory of Systems and Synthetic Biology,
            Wageningen University, Stippeneng 4, Wageningen, Gelderland 6708 WE,
            The Netherlands
REFERENCE   3  (bases 1 to 3957)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb 
            Biotechnol"; date: "2020"; volume: "13"; issue: "6"; pages: 
            "1793-1806"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-JAN-2021) Laboratory of Systems and Synthetic Biology, 
            Wageningen University, Stippeneng 4, Wageningen, Gelderland 6708 WE,
            The Netherlands"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     regulatory      23..57
                     /label=promoter BBa_J23105
                     /note="promoter BBa_J23105"
                     /regulatory_class="promoter"
     regulatory      66..77
                     /label=RBS.20
                     /note="RBS.20"
                     /regulatory_class="ribosome_binding_site"
     CDS             84..797
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pedelacq et al., 2006)"
     misc_feature    804..824
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      855..949
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1022..1113
                     /label=AmpR promoter
     CDS             1114..1971
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      1978..2017
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2164..2272
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(2286..2945)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(2946..3717)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     terminator      complement(3832..3918)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gaattcgcgg ccgcttctag agtttacggc tagctcagtc ctaggtacta tgctagctac
       61 tagagtattt aaggctgtac tagatgcgta aaggcgaaga actgttcacg ggcgtagttc
      121 cgattctggt cgagctggac ggcgatgtga acggtcataa gtttagcgtt cgcggtgaag
      181 gtgagggcga cgcgaccaac ggcaaactga ccctgaagtt catctgcacc accggtaaac
      241 tgccggtgcc ttggccgacc ttggtgacga cgttgacgta tggcgtgcag tgttttgcgc
      301 gttatccgga ccacatgaaa caacacgatt tcttcaaatc tgcgatgccg gagggttacg
      361 tccaggagcg taccatttcc ttcaaggatg atggctacta caaaactcgc gcagaggtta
      421 agtttgaagg tgacacgctg gtcaatcgta tcgaattgaa gggtatcgac tttaaagagg
      481 atggtaacat tctgggccat aaactggagt ataacttcaa cagccataat gtttacatta
      541 cggcagacaa gcaaaagaac ggcatcaagg ccaatttcaa gattcgccac aatgttgagg
      601 acggtagcgt ccaactggcc gaccattacc agcagaacac cccaattggt gacggtccgg
      661 ttttgctgcc ggataatcac tatctgagca cccaaagcgt gctgagcaaa gatccgaacg
      721 aaaaacgtga tcacatggtc ctgctggaat ttgtgaccgc tgcgggcatc acccacggta
      781 tggatgaact gtacaaataa taatactagt agcggccgct gcagtccggc aaaaaagggc
      841 aaggtctagt cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga
      901 tttgttcaga acgctcggtt gccgccgggc gttttttatt ggtgagaatc caggggtccc
      961 caataattac gatttaaatt agtagcccgc ctaatgagcg ggcttttttt taattcccct
     1021 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     1081 taaatgcttc aataatattg aaaaaggaag agtatgagca ttcagcattt tcgtgtggcg
     1141 ctgattccgt tttttgcggc gttttgcctg ccggtgtttg cgcatccgga aaccctggtg
     1201 aaagtgaaag atgcggaaga tcaactgggt gcgcgcgtgg gctatattga actggatctg
     1261 aacagcggca aaattctgga atcttttcgt ccggaagaac gttttccgat gatgagcacc
     1321 tttaaagtgc tgctgtgcgg tgcggttctg agccgtgtgg atgcgggcca ggaacaactg
     1381 ggccgtcgta ttcattatag ccagaacgat ctggtggaat atagcccggt gaccgaaaaa
     1441 catctgaccg atggcatgac cgtgcgtgaa ctgtgcagcg cggcgattac catgagcgat
     1501 aacaccgcgg cgaacctgct gctgacgacc attggcggtc cgaaagaact gaccgcgttt
     1561 ctgcataaca tgggcgatca tgtgacccgt ctggatcgtt gggaaccgga actgaacgaa
     1621 gcgattccga acgatgaacg tgataccacc atgccggcag caatggcgac caccctgcgt
     1681 aaactgctga cgggtgagct gctgaccctg gcaagccgcc agcaactgat tgattggatg
     1741 gaagcggata aagtggcggg tccgctgctg cgtagcgcgc tgccggctgg ctggtttatt
     1801 gcggataaaa gcggtgcggg cgaacgtggc agccgtggca ttattgcggc gctgggcccg
     1861 gatggtaaac cgagccgtat tgtggtgatt tataccaccg gcagccaggc gacgatggat
     1921 gaacgtaacc gtcagattgc ggaaattggc gcgagcctga ttaaacattg gtaaaccgat
     1981 acaattaaag gctccttttg gagccttttt ttttggacga cccttgtcct tttccgctgc
     2041 ataaccctgc ttcggggtca ttatagcgat tttttcggta tatccatcct ttttcgcacg
     2101 atatacagga ttttgccaaa gggttcgtgt agactttcct tggtgtatcc aacggcgtca
     2161 gccgggcagg ataggtgaag taggcccacc cgcgagcggg tgttccttct tcactgtccc
     2221 ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc cgtaggccgg
     2281 ccctaccggc gcggcagcgt tacccgtgtc ggcggctcca acggctcgcc atcgtccaga
     2341 aaacacggct catcgggcat cggcaggcgc tgctgcccgc gccgttccca ttcctccgtt
     2401 tcggtcaagg ctggcaggtc tggttccatg cccggaatgc cgggctggct gggcggctcc
     2461 tcgccggggc cggtcggtag ttgctgctcg cccggataca gggtcgggat gcggcgcagg
     2521 tcgccatgcc ccaacagcga ttcgtcctgg tcgtcgtgat caaccaccac ggcggcactg
     2581 aacaccgaca ggcgcaactg gtcgcggggc tggccccacg ccacgcggtc attgaccacg
     2641 taggccgaca cggtgccggg gccgttgagc ttcacgacgg agatccagcg ctcggccacc
     2701 aagtccttga ctgcgtattg gaccgtccgc aaagaacgtc cgatgagctt ggaaagtgtc
     2761 ttctggctga ccaccacggc gttctggtgg cccatctgcg ccacgaggtg atgcagcagc
     2821 attgccgccg tgggtttcct cgcaataagc ccggcccacg cctcatgcgc tttgcgttcc
     2881 gtttgcaccc agtgaccggg cttgttcttg gcttgaatgc cgatttctct ggactgcgtg
     2941 gccatgctta tctccatgcg gtaggggtgc cgcacggttg cggcaccatg cgcaatcagc
     3001 tgcaactttt cggcagcgcg acaacaatta tgcgttgcgt aaaagtggca gtcaattaca
     3061 gattttcttt aacctacgca atgagctatt gcggggggtg ccgcaatgag ctgttgcgta
     3121 cccccctttt ttaagttgtt gatttttaag tctttcgcat ttcgccctat atctagttct
     3181 ttggtgccca aagaagggca cccctgcggg gttcccccac gccttcggcg cggctccccc
     3241 tccggcaaaa agtggcccct ccggggcttg ttgatcgact gcgcggcctt cggccttgcc
     3301 caaggtggcg ctgccccctt ggaacccccg cactcgccgc cgtgaggctc ggggggcagg
     3361 cgggcgggct tcgcccttcg actgccccca ctcgcatagg cttgggtcgt tccaggcgcg
     3421 tcaaggccaa gccgctgcgc ggtcgctgcg cgagccttga cccgccttcc acttggtgtc
     3481 caaccggcaa gcgaagcgcg caggccgcag gccggaggct tttccccaga gaaaattaaa
     3541 aaaattgatg gggcaaggcc gcaggccgcg cagttggagc cggtgggtat gtggtcgaag
     3601 gctgggtagc cggtgggcaa tccctgtggt caagctcgtg ggcaggcgca gcctgtccat
     3661 cagcttgtcc agcagggttg tccacgggcc gagcgaagcg agccagccgg tggccgctcg
     3721 cggccatcgt ccacatatcc acgggctggc aagggagcgc agcgaccgcg cagggcgaag
     3781 cccggagagc aagcccgtag ggggggcgcg cccagctgtc tagggcggcg gatttgtcct
     3841 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt ctttcgactg
     3901 agcctttcgt tttatttgat gcctttaatt aatagctttc gctaaggatg atttctg
//
