LOCUS       40924_39643        5153 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSEVA651, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent 
            platform for analysis and deployment of complex prokaryotic 
            phenotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 5153)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5153)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ApE v. v2.0.40
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..32
                     /label=R24
                     /note="R24"
     misc_feature    56..112
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(121..144)
                     /label=F24
                     /note="F24"
     terminator      155..249
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        280..308
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             497..1027
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     oriT            1241..1349
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(1363..2211)
                     /codon_start=1
                     /label=RSF1010 RepC
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="VVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(2201..3037)
                     /codon_start=1
                     /label=RSF1010 RepA
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(3551..4519)
                     /codon_start=1
                     /label=RSF1010 RepB
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     promoter        complement(4543..4573)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     rep_origin      4629..5023
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(5061..5147)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc
      121 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg
      181 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
      241 ggtgagaatc caggggtccc caataattac gatttaaatt tgacataagc ctgttcggtt
      301 cgtaaactgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa
      361 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttgta
      421 cagcctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
      481 ttatggagca gcaacgatgt tacgcagcag caacgatgtt acgcagcagg gcagtcgccc
      541 taaaacaaag ttaggtggct caagtatggg catcattcgc acatgtaggc tcggccctga
      601 ccaagtcaaa tccatgcggg ctgctcttga tcttttcggt cgtgagttcg gagacgtagc
      661 cacctactcc caacatcagc cggactccga ttacctcggg aacttgctcc gtagtaagac
      721 attcatcgcg cttgctgcct tcgaccaaga agcggttgtt ggcgctctcg cggcttacgt
      781 tctgcccaag tttgagcagc cgcgtagtga gatctatatc tatgatctcg cagtctccgg
      841 agagcaccgg aggcagggca ttgccaccgc gctcatcaat ctcctcaagc atgaggccaa
      901 cgcgcttggt gcttatgtga tctacgtgca agcagattac ggtgacgatc ccgcagtggc
      961 tctctataca aagttgggca tacgggaaga agtgatgcac tttgatatcg acccaagtac
     1021 cgccacctaa caattcgttc aagccgagat cggcttcccg gccgcggagt tgttcggtaa
     1081 attggacaac ggtccgcgcg ttgtcctttt ccgctgcata accctgcttc ggggtcatta
     1141 tagcgatttt ttcggtatat ccatcctttt tcgcacgata tacaggattt tgccaaaggg
     1201 ttcgtgtaga ctttccttgg tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag
     1261 gcccacccgc gagcgggtgt tccttcttca ctgtccctta ttcgcacctg gcggtgctca
     1321 acgggaatcc tgctctgcga ggctggccgt aggccggcct cagcctgccg ccttgggccg
     1381 ggtgatgtcg tacttgcccg ccgcgaactc ggttaccgtc cagcccagcg cgaccagctc
     1441 cggcaacgcc tcgcgcaccc gctggcggcg cttgcgcatg gtcgaaccac tggcctctga
     1501 cggccagaca tagccgcaca aggtatctat ggaagccttg ccggttttgc cggggtcgat
     1561 ccagccacac agccgctggt gcagcaggcg ggcggtttcg ctgtccagcg cccgcacctc
     1621 gtccatgctg atgcgcacat gctggccgcc acccatgacg gcctgcgcga tcaaggggtt
     1681 cagggccacg tacaggcgcc cgtccgcctc gtcgctggcg tactccgaca gcagccgaaa
     1741 cccctgccgc ttgcggccat tctgggcgat gatggatacc ttccaaaggc gctcgatgca
     1801 gtcctgtatg tgcttgagcg ccccaccact atcgacctct gccccgattt cctttgccag
     1861 cgcccgatag ctacctttga ccacatggca ttcagcggtg acggcctccc acttgggttc
     1921 caggaacagc cggagctgcc gtccgccttc ggtcttgggt tccgggccaa gcactaggcc
     1981 attaggccca gccatggcca ccagcccttg caggatgcgc agatcatcag cgcccagcgg
     2041 ctccgggccg ctgaactcga tccgcttgcc gtcgccgtag tcatacgtca cgtccagctt
     2101 gctgcgcttg cgctcgcccc gcttgagggc acggaacagg ccgggggcca gacagtgcgc
     2161 cgggtcgtgc cggacgtggc tgaggctgtg cttgttctta ggcttcacca cggggcaccc
     2221 ccttgctctt gcgctgcctc tccagcacgg cgggcttgag caccccgccg tcatgccgcc
     2281 tgaaccaccg atcagcgaac ggtgcgccat agttggcctt gctcacaccg aagcggacga
     2341 agaaccggcg ctggtcgtcg tccacacccc attcctcggc ctcggcgctg gtcatgctcg
     2401 acaggtagga ctgccagcgg atgttatcga ccagtaccga gctgccccgg ctggcctgct
     2461 gctggtcgcc tgcgcccatc atggccgcgc ccttgctggc atggtgcagg aacacgatag
     2521 agcacccggt atcggcggcg atggcctcca tgcgaccgat gacctgggcc atggggccgc
     2581 tggcgttttc ttcctcgatg tggaaccggc gcagcgtgtc cagcaccatc aggcggcggc
     2641 cctcggcggc gcgcttgagg ccgtcgaacc actccggggc catgatgttg ggcaggctgc
     2701 cgatcagcgg ctggatcagc aggccgtcag ccacggcttg ccgttcctcg gcgctgaggt
     2761 gcgccccaag ggcgtgcagg cggtgatgaa tggcggtggg cgggtcttcg gcgggcaggt
     2821 agatcaccgg gccggtgggc agttcgccca cctccagcag atccggcccg cctgcaatct
     2881 gtgcggccag ttgcagggcc agcatggatt taccggcacc accgggcgac accagcgccc
     2941 cgaccgtacc ggccaccatg ttgggcaaaa cgtagtccag cggtggcggc gctgctgcga
     3001 acgcctccag aatattgata ggcttatggg tagccattga ttgcctcctt tgcaggcagt
     3061 tggtggttag gcgctggcgg ggtcactacc cccgccctgc gccgctctga gttcttccag
     3121 gcactcgcgc agcgcctcgt attcgtcgtc ggtcagccag aacttgcgct gacgcatccc
     3181 tttggccttc atgcgctcgg catatcgcgc ttggcgtaca gcgtcagggc tggccagcag
     3241 gtcgccggtc tgcttgtcct tttggtcttt catatcagtc accgagaaac ttgccggggc
     3301 cgaaaggctt gtcttcgcgg aacaaggaca aggtgcagcc gtcaaggtta aggctggcca
     3361 tatcagcgac tgaaaagcgg ccagcctcgg ccttgtttga cgtataacca aagccaccgg
     3421 gcaaccaata gcccttgtca cttttgatca ggtagaccga ccctgaagcg cttttttcgt
     3481 attccataaa acccccttct gtgcgtgagt actcatagta taacaggcgt gagtaccaac
     3541 gcaagcacta catgctgaaa tctggcccgc ccctgtccat gcctcgctgg cggggtgccg
     3601 gtgcccgtgc cagctcggcc cgcgcaagct ggacgctggg cagacccatg accttgctga
     3661 cggtgcgctc gatgtaatcc gcttcgtggc cgggcttgcg ctctgccagc gctgggctgg
     3721 cctcggccat ggccttgccg atttcctcgg cactgcggcc ccggctggcc agcttctgcg
     3781 cggcgataaa gtcgcacttg ctgaggtcat caccgaagcg cttgaccagc ccggccatct
     3841 cgctgcggta ctcgtccagc gccgtgcgcc ggtggcggct aagctgccgc tcgggcagtt
     3901 cgaggctggc cagcctgcgg gccttctcct gctgccgctg ggcctgctcg atctgctggc
     3961 cagcctgctg caccagcgcc gggccagcgg tggcggtctt gcccttggat tcacgcagca
     4021 gcacccacgg ctgataaccg gcgcgggtgg tgtgcttgtc cttgcggttg gtgaagcccg
     4081 ccaagcggcc atagtggcgg ctgtcggcgc tggccgggtc ggcgtcgtac tcgctggcca
     4141 gcgtccgggc aatctgcccc cgaagttcac cgcctgcggc gtcggccacc ttgacccatg
     4201 cctgatagtt cttcgggctg gtttccacta ccagggcagg ctcccggccc tcggctttca
     4261 tgtcatccag gtcaaactcg ctgaggtcgt ccaccagcac cagaccatgc cgctcctgct
     4321 cggcgggcct gatatacacg tcattgccct gggcattcat ccgcttgagc catggcgtgt
     4381 tctggagcac ttcggcggct gaccattccc ggttcatcat ctggccggtg gtggcgtccc
     4441 tgacgccgat atcgaagcgc tcacagccca tggccttgag ctgtcggcct atggcctgca
     4501 aagtcctgtc gttcttcatc gggccaccaa gcgattccca cacattatac gagccggaag
     4561 cataaagtgt aaagcctaga tccgaaggat gagccgggct gaatgatcga ccgagacagg
     4621 ccctgcgggg ctgcacacgc gcccccaccc ttcgggtagg gggaaaggcc gctaaagcgg
     4681 ctaaaagcgc tccagcgtat ttctgcgggg tttggtgtgg ggtttagcgg gctttgcccg
     4741 cctttccccc tgccgcgcag cggtggggcg gtgtgtagcc tagcgcagcg aatagaccag
     4801 ctatccggcc tctggccggg catattgggc aagggcagca gcgccccaca agggcgctga
     4861 taaccgcgcc tagtggatta ttcttagata atcatggatg gatttttcca acaccccgcc
     4921 agcccccgcc cctgctgggt ttgcaggttt gggggcgtga cagttattgc aggggttcgt
     4981 gacagttatt gcaggggggc gtgacagtta ttgcaggggt tcgtgacagt tagggcgcgc
     5041 ccagctgtct agggcggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag
     5101 ataaaacgaa aggcccagtc tttcgactga gcctttcgtt ttatttgatg cct
//
