LOCUS       40924_39638        3897 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSEVA643, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent 
            platform for analysis and deployment of complex prokaryotic 
            phenotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 3897)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3897)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ApE v. v2.0.40
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    125..146
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        161..191
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    199..215
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     223..239
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    249..305
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(309..325)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(329..352)
                     /label=F24
                     /note="F24"
     terminator      604..698
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        729..757
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             946..1476
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     oriT            1690..1798
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      1892..2480
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2537..3367)
                     /codon_start=1
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
                     /translation="VASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      3381..3732
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      complement(3805..3891)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
       61 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      121 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
      181 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
      241 atgattacga attcgagctc ggtacccggg gatcctctag agtcgacctg caggcatgca
      301 agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      361 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      421 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
      481 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
      541 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgaactagtc
      601 ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa
      661 cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aggggtcccc aataattacg
      721 atttaaattt gacataagcc tgttcggttc gtaaactgta atgcaagtag cgtatgcgct
      781 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
      841 tcatggcttg ttatgactgt ttttttgtac agcctatgcc tcgggcatcc aagcagcaag
      901 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagc
      961 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taggtggctc aagtatgggc
     1021 atcattcgca catgtaggct cggccctgac caagtcaaat ccatgcgggc tgctcttgat
     1081 cttttcggtc gtgagttcgg agacgtagcc acctactccc aacatcagcc ggactccgat
     1141 tacctcggga acttgctccg tagtaagaca ttcatcgcgc ttgctgcctt cgaccaagaa
     1201 gcggttgttg gcgctctcgc ggcttacgtt ctgcccaagt ttgagcagcc gcgtagtgag
     1261 atctatatct atgatctcgc agtctccgga gagcaccgga ggcagggcat tgccaccgcg
     1321 ctcatcaatc tcctcaagca tgaggccaac gcgcttggtg cttatgtgat ctacgtgcaa
     1381 gcagattacg gtgacgatcc cgcagtggct ctctatacaa agttgggcat acgggaagaa
     1441 gtgatgcact ttgatatcga cccaagtacc gccacctaac aattcgttca agccgagatc
     1501 ggcttcccgg ccgcggagtt gttcggtaaa ttggacaacg gtccgcgcgt tgtccttttc
     1561 cgctgcataa ccctgcttcg gggtcattat agcgattttt tcggtatatc catccttttt
     1621 cgcacgatat acaggatttt gccaaagggt tcgtgtagac tttccttggt gtatccaacg
     1681 gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac
     1741 tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag gctggccgta
     1801 ggccggccga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1861 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1921 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1981 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     2041 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     2101 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     2161 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     2221 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     2281 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggcat ccggtaagcg
     2341 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     2401 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     2461 gggggcggag cctatggaaa aacgccagca acgcggccgt gaaaggcagg ccggtccgtg
     2521 gtggccacgg cctctaggcc agatccagcg gcatctgggt tagtcgagcg cgggccgctt
     2581 cccatgtctc accagggcga gcctgtttcg cgatctcagc atctgaaatc ttcccggcct
     2641 tgcgcttcgc tggggcctta cccaccgcct tggcgggctt cttcggtcca aaactgaaca
     2701 acagatgtgt gaccttgcgc ccggtctttc gctgcgccca ctccacctgt agcgggctgt
     2761 gctcgttgat ctgcgtcacg gctggatcaa gcactcgcaa cttgaagtcc ttgatcgagg
     2821 gataccggcc ttccagttga aaccactttc gcagctggtc aatttctatt tcgcgctggc
     2881 cgatgctgtc ccattgcatg agcagctcgt aaagcctgat cgcgtgggtg ctgtccatct
     2941 tggccacgtc agccaaggcg tatttggtga actgtttggt gagttccgtc aggtacggca
     3001 gcatgtcttt ggtgaacctg agttctacac ggccctcacc ctcccggtag atgattgttt
     3061 gcacccagcc ggtaatcatc acactcggtc ttttcccctt gccattgggc tcttgggtta
     3121 accggacttc ccgccgtttc aggcgcaggg ccgcttcttt gagctggttg taggaagatt
     3181 cgatagggac acccgccatc gtcgctatgt cctccgccgt cactgaatac atcacttcat
     3241 cggtgacagg ctcgctcctc ttcacctggc taatacaggc cagaacgatc cgctgttcct
     3301 gaacactgag gcgatacgcg gcctcgacca gggcattgct tttgtaaacc attgggggtg
     3361 aggccacgtt cgacattcct tgtgtataag gggacactgt atctgcgtcc cacaatacaa
     3421 caaatccgtc cctttacaac aacaaatccg tcccttctta acaacaaatc cgtcccttaa
     3481 tggcaacaaa tccgtccctt tttaaactct acaggccacg gattacgtgg cctgtagacg
     3541 tcctaaaagg tttaaaaggg aaaaggaaga aaagggtgga aacgcaaaaa acgcaccact
     3601 acgtggcccc gttggggccg catttgtgcc cctgaagggg cgggggaggc gtctgggcaa
     3661 tccccgtttt accagtcccc tatcgccgcc tgagagggcg caggaagcga gtaatcaggg
     3721 tatcgaggcg gattcaccct tggcgtccaa ccagcggcac cagcggcgcc tgagaggggc
     3781 gcgcccagct gtctagggcg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     3841 acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcct
//
