LOCUS       40924_39628        3448 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSEVA641, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent 
            platform for analysis and deployment of complex prokaryotic 
            phenotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 3448)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3448)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ApE v. v2.0.40
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..32
                     /label=R24
                     /note="R24"
     misc_feature    56..112
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(121..144)
                     /label=F24
                     /note="F24"
     terminator      155..249
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        280..308
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             497..1027
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     oriT            1241..1349
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      1443..2031
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2088..2918)
                     /codon_start=1
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
                     /translation="VASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      2932..3283
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      complement(3356..3442)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc
      121 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg
      181 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
      241 ggtgagaatc caggggtccc caataattac gatttaaatt tgacataagc ctgttcggtt
      301 cgtaaactgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa
      361 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttgta
      421 cagcctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
      481 ttatggagca gcaacgatgt tacgcagcag caacgatgtt acgcagcagg gcagtcgccc
      541 taaaacaaag ttaggtggct caagtatggg catcattcgc acatgtaggc tcggccctga
      601 ccaagtcaaa tccatgcggg ctgctcttga tcttttcggt cgtgagttcg gagacgtagc
      661 cacctactcc caacatcagc cggactccga ttacctcggg aacttgctcc gtagtaagac
      721 attcatcgcg cttgctgcct tcgaccaaga agcggttgtt ggcgctctcg cggcttacgt
      781 tctgcccaag tttgagcagc cgcgtagtga gatctatatc tatgatctcg cagtctccgg
      841 agagcaccgg aggcagggca ttgccaccgc gctcatcaat ctcctcaagc atgaggccaa
      901 cgcgcttggt gcttatgtga tctacgtgca agcagattac ggtgacgatc ccgcagtggc
      961 tctctataca aagttgggca tacgggaaga agtgatgcac tttgatatcg acccaagtac
     1021 cgccacctaa caattcgttc aagccgagat cggcttcccg gccgcggagt tgttcggtaa
     1081 attggacaac ggtccgcgcg ttgtcctttt ccgctgcata accctgcttc ggggtcatta
     1141 tagcgatttt ttcggtatat ccatcctttt tcgcacgata tacaggattt tgccaaaggg
     1201 ttcgtgtaga ctttccttgg tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag
     1261 gcccacccgc gagcgggtgt tccttcttca ctgtccctta ttcgcacctg gcggtgctca
     1321 acgggaatcc tgctctgcga ggctggccgt aggccggccg ataatctcat gaccaaaatc
     1381 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     1441 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     1501 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     1561 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     1621 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     1681 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     1741 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     1801 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     1861 gggagaaagg cggacaggca tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     1921 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     1981 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     2041 aacgcggccg tgaaaggcag gccggtccgt ggtggccacg gcctctaggc cagatccagc
     2101 ggcatctggg ttagtcgagc gcgggccgct tcccatgtct caccagggcg agcctgtttc
     2161 gcgatctcag catctgaaat cttcccggcc ttgcgcttcg ctggggcctt acccaccgcc
     2221 ttggcgggct tcttcggtcc aaaactgaac aacagatgtg tgaccttgcg cccggtcttt
     2281 cgctgcgccc actccacctg tagcgggctg tgctcgttga tctgcgtcac ggctggatca
     2341 agcactcgca acttgaagtc cttgatcgag ggataccggc cttccagttg aaaccacttt
     2401 cgcagctggt caatttctat ttcgcgctgg ccgatgctgt cccattgcat gagcagctcg
     2461 taaagcctga tcgcgtgggt gctgtccatc ttggccacgt cagccaaggc gtatttggtg
     2521 aactgtttgg tgagttccgt caggtacggc agcatgtctt tggtgaacct gagttctaca
     2581 cggccctcac cctcccggta gatgattgtt tgcacccagc cggtaatcat cacactcggt
     2641 cttttcccct tgccattggg ctcttgggtt aaccggactt cccgccgttt caggcgcagg
     2701 gccgcttctt tgagctggtt gtaggaagat tcgataggga cacccgccat cgtcgctatg
     2761 tcctccgccg tcactgaata catcacttca tcggtgacag gctcgctcct cttcacctgg
     2821 ctaatacagg ccagaacgat ccgctgttcc tgaacactga ggcgatacgc ggcctcgacc
     2881 agggcattgc ttttgtaaac cattgggggt gaggccacgt tcgacattcc ttgtgtataa
     2941 ggggacactg tatctgcgtc ccacaataca acaaatccgt ccctttacaa caacaaatcc
     3001 gtcccttctt aacaacaaat ccgtccctta atggcaacaa atccgtccct ttttaaactc
     3061 tacaggccac ggattacgtg gcctgtagac gtcctaaaag gtttaaaagg gaaaaggaag
     3121 aaaagggtgg aaacgcaaaa aacgcaccac tacgtggccc cgttggggcc gcatttgtgc
     3181 ccctgaaggg gcgggggagg cgtctgggca atccccgttt taccagtccc ctatcgccgc
     3241 ctgagagggc gcaggaagcg agtaatcagg gtatcgaggc ggattcaccc ttggcgtcca
     3301 accagcggca ccagcggcgc ctgagagggg cgcgcccagc tgtctagggc ggcggatttg
     3361 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg
     3421 actgagcctt tcgttttatt tgatgcct
//
