LOCUS       40924_39613        3001 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSEVA631, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent 
            platform for analysis and deployment of complex prokaryotic 
            phenotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 3001)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3001)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ApE v. v2.0.40
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..32
                     /label=R24
                     /note="R24"
     misc_feature    56..112
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(121..144)
                     /label=F24
                     /note="F24"
     terminator      155..249
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        280..308
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             497..1027
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     oriT            1241..1349
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(1363..2022)
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      complement(2023..2794)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     terminator      complement(2909..2995)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc
      121 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg
      181 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
      241 ggtgagaatc caggggtccc caataattac gatttaaatt tgacataagc ctgttcggtt
      301 cgtaaactgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa
      361 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttgta
      421 cagcctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
      481 ttatggagca gcaacgatgt tacgcagcag caacgatgtt acgcagcagg gcagtcgccc
      541 taaaacaaag ttaggtggct caagtatggg catcattcgc acatgtaggc tcggccctga
      601 ccaagtcaaa tccatgcggg ctgctcttga tcttttcggt cgtgagttcg gagacgtagc
      661 cacctactcc caacatcagc cggactccga ttacctcggg aacttgctcc gtagtaagac
      721 attcatcgcg cttgctgcct tcgaccaaga agcggttgtt ggcgctctcg cggcttacgt
      781 tctgcccaag tttgagcagc cgcgtagtga gatctatatc tatgatctcg cagtctccgg
      841 agagcaccgg aggcagggca ttgccaccgc gctcatcaat ctcctcaagc atgaggccaa
      901 cgcgcttggt gcttatgtga tctacgtgca agcagattac ggtgacgatc ccgcagtggc
      961 tctctataca aagttgggca tacgggaaga agtgatgcac tttgatatcg acccaagtac
     1021 cgccacctaa caattcgttc aagccgagat cggcttcccg gccgcggagt tgttcggtaa
     1081 attggacaac ggtccgcgcg ttgtcctttt ccgctgcata accctgcttc ggggtcatta
     1141 tagcgatttt ttcggtatat ccatcctttt tcgcacgata tacaggattt tgccaaaggg
     1201 ttcgtgtaga ctttccttgg tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag
     1261 gcccacccgc gagcgggtgt tccttcttca ctgtccctta ttcgcacctg gcggtgctca
     1321 acgggaatcc tgctctgcga ggctggccgt aggccggccc taccggcgcg gcagcgttac
     1381 ccgtgtcggc ggctccaacg gctcgccatc gtccagaaaa cacggctcat cgggcatcgg
     1441 caggcgctgc tgcccgcgcc gttcccattc ctccgtttcg gtcaaggctg gcaggtctgg
     1501 ttccatgccc ggaatgccgg gctggctggg cggctcctcg ccggggccgg tcggtagttg
     1561 ctgctcgccc ggatacaggg tcgggatgcg gcgcaggtcg ccatgcccca acagcgattc
     1621 gtcctggtcg tcgtgatcaa ccaccacggc ggcactgaac accgacaggc gcaactggtc
     1681 gcggggctgg ccccacgcca cgcggtcatt gaccacgtag gccgacacgg tgccggggcc
     1741 gttgagcttc acgacggaga tccagcgctc ggccaccaag tccttgactg cgtattggac
     1801 cgtccgcaaa gaacgtccga tgagcttgga aagtgtcttc tggctgacca ccacggcgtt
     1861 ctggtggccc atctgcgcca cgaggtgatg cagcagcatt gccgccgtgg gtttcctcgc
     1921 aataagcccg gcccacgcct catgcgcttt gcgttccgtt tgcacccagt gaccgggctt
     1981 gttcttggct tgaatgccga tttctctgga ctgcgtggcc atgcttatct ccatgcggta
     2041 ggggtgccgc acggttgcgg caccatgcgc aatcagctgc aacttttcgg cagcgcgaca
     2101 acaattatgc gttgcgtaaa agtggcagtc aattacagat tttctttaac ctacgcaatg
     2161 agctattgcg gggggtgccg caatgagctg ttgcgtaccc ccctttttta agttgttgat
     2221 ttttaagtct ttcgcatttc gccctatatc tagttctttg gtgcccaaag aagggcaccc
     2281 ctgcggggtt cccccacgcc ttcggcgcgg ctccccctcc ggcaaaaagt ggcccctccg
     2341 gggcttgttg atcgactgcg cggccttcgg ccttgcccaa ggtggcgctg cccccttgga
     2401 acccccgcac tcgccgccgt gaggctcggg gggcaggcgg gcgggcttcg cccttcgact
     2461 gcccccactc gcataggctt gggtcgttcc aggcgcgtca aggccaagcc gctgcgcggt
     2521 cgctgcgcga gccttgaccc gccttccact tggtgtccaa ccggcaagcg aagcgcgcag
     2581 gccgcaggcc ggaggctttt ccccagagaa aattaaaaaa attgatgggg caaggccgca
     2641 ggccgcgcag ttggagccgg tgggtatgtg gtcgaaggct gggtagccgg tgggcaatcc
     2701 ctgtggtcaa gctcgtgggc aggcgcagcc tgtccatcag cttgtccagc agggttgtcc
     2761 acgggccgag cgaagcgagc cagccggtgg ccgctcgcgg ccatcgtcca catatccacg
     2821 ggctggcaag ggagcgcagc gaccgcgcag ggcgaagccc ggagagcaag cccgtagggg
     2881 gggcgcgccc agctgtctag ggcggcggat ttgtcctact caggagagcg ttcaccgaca
     2941 aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc
     3001 t
//
