LOCUS       40924_39593        5612 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSEVA551, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent 
            platform for analysis and deployment of complex prokaryotic 
            phenotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 5612)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5612)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ApE v. v2.0.40
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..32
                     /label=R24
                     /note="R24"
     misc_feature    56..112
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(121..144)
                     /label=F24
                     /note="F24"
     terminator      155..249
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             364..1551
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     oriT            1700..1808
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(1822..2670)
                     /codon_start=1
                     /label=RSF1010 RepC
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="VVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(2660..3496)
                     /codon_start=1
                     /label=RSF1010 RepA
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(4010..4978)
                     /codon_start=1
                     /label=RSF1010 RepB
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     promoter        complement(5002..5032)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     rep_origin      5088..5482
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(5520..5606)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc
      121 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg
      181 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
      241 ggtgagaatc caggggtccc caataattac gatttacgta tttaaatttg acagcttatc
      301 atcgataaac tgtaatgcgg tagtttatca cagttaaatt gctaacgcag tcaggcaccg
      361 tgtatgaaat ctaacaatgc gctcatcgtc atcctcggca ccgtcaccct ggatgctgta
      421 ggcataggct tggttatgcc ggtactgccg ggcctcttgc gggatatcgt ccattccgac
      481 agcatcgcca gtcactatgg cgtgctgcta gcgctatatg cgttgatgca atttctatgc
      541 gcacccgttc tcggagcact gtccgaccgc tttggccgcc gcccagtcct gctcgcttcg
      601 ctacttggag ccactatcga ctacgcgatc atggcgacca cacccgtcct gtggattctc
      661 tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat
      721 atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt
      781 ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccctg
      841 cacgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc
      901 ctaatgcagg agtcgcataa gggagagcgc cgtccgatgc ccttgagagc cttcaaccca
      961 gtcagctcct tccggtgggc gcggggcatg accattgtgg ccgcacttat gactgtcttc
     1021 tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac
     1081 cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac
     1141 gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc
     1201 attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga
     1261 ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg
     1321 ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg
     1381 ctcgcggctc ttaccagcct aacttcgatc actggaccgc tgatcgtcac ggcgatttat
     1441 gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggcgccgc cctatacctt
     1501 gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac ctgagacaat
     1561 tgtccttttc cgctgcataa ccctgcttcg gggtcattat agcgattttt tcggtatatc
     1621 catccttttt cgcacgatat acaggatttt gccaaagggt tcgtgtagac tttccttggt
     1681 gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt
     1741 ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag
     1801 gctggccgta ggccggcctc agcctgccgc cttgggccgg gtgatgtcgt acttgcccgc
     1861 cgcgaactcg gttaccgtcc agcccagcgc gaccagctcc ggcaacgcct cgcgcacccg
     1921 ctggcggcgc ttgcgcatgg tcgaaccact ggcctctgac ggccagacat agccgcacaa
     1981 ggtatctatg gaagccttgc cggttttgcc ggggtcgatc cagccacaca gccgctggtg
     2041 cagcaggcgg gcggtttcgc tgtccagcgc ccgcacctcg tccatgctga tgcgcacatg
     2101 ctggccgcca cccatgacgg cctgcgcgat caaggggttc agggccacgt acaggcgccc
     2161 gtccgcctcg tcgctggcgt actccgacag cagccgaaac ccctgccgct tgcggccatt
     2221 ctgggcgatg atggatacct tccaaaggcg ctcgatgcag tcctgtatgt gcttgagcgc
     2281 cccaccacta tcgacctctg ccccgatttc ctttgccagc gcccgatagc tacctttgac
     2341 cacatggcat tcagcggtga cggcctccca cttgggttcc aggaacagcc ggagctgccg
     2401 tccgccttcg gtcttgggtt ccgggccaag cactaggcca ttaggcccag ccatggccac
     2461 cagcccttgc aggatgcgca gatcatcagc gcccagcggc tccgggccgc tgaactcgat
     2521 ccgcttgccg tcgccgtagt catacgtcac gtccagcttg ctgcgcttgc gctcgccccg
     2581 cttgagggca cggaacaggc cgggggccag acagtgcgcc gggtcgtgcc ggacgtggct
     2641 gaggctgtgc ttgttcttag gcttcaccac ggggcacccc cttgctcttg cgctgcctct
     2701 ccagcacggc gggcttgagc accccgccgt catgccgcct gaaccaccga tcagcgaacg
     2761 gtgcgccata gttggccttg ctcacaccga agcggacgaa gaaccggcgc tggtcgtcgt
     2821 ccacacccca ttcctcggcc tcggcgctgg tcatgctcga caggtaggac tgccagcgga
     2881 tgttatcgac cagtaccgag ctgccccggc tggcctgctg ctggtcgcct gcgcccatca
     2941 tggccgcgcc cttgctggca tggtgcagga acacgataga gcacccggta tcggcggcga
     3001 tggcctccat gcgaccgatg acctgggcca tggggccgct ggcgttttct tcctcgatgt
     3061 ggaaccggcg cagcgtgtcc agcaccatca ggcggcggcc ctcggcggcg cgcttgaggc
     3121 cgtcgaacca ctccggggcc atgatgttgg gcaggctgcc gatcagcggc tggatcagca
     3181 ggccgtcagc cacggcttgc cgttcctcgg cgctgaggtg cgccccaagg gcgtgcaggc
     3241 ggtgatgaat ggcggtgggc gggtcttcgg cgggcaggta gatcaccggg ccggtgggca
     3301 gttcgcccac ctccagcaga tccggcccgc ctgcaatctg tgcggccagt tgcagggcca
     3361 gcatggattt accggcacca ccgggcgaca ccagcgcccc gaccgtaccg gccaccatgt
     3421 tgggcaaaac gtagtccagc ggtggcggcg ctgctgcgaa cgcctccaga atattgatag
     3481 gcttatgggt agccattgat tgcctccttt gcaggcagtt ggtggttagg cgctggcggg
     3541 gtcactaccc ccgccctgcg ccgctctgag ttcttccagg cactcgcgca gcgcctcgta
     3601 ttcgtcgtcg gtcagccaga acttgcgctg acgcatccct ttggccttca tgcgctcggc
     3661 atatcgcgct tggcgtacag cgtcagggct ggccagcagg tcgccggtct gcttgtcctt
     3721 ttggtctttc atatcagtca ccgagaaact tgccggggcc gaaaggcttg tcttcgcgga
     3781 acaaggacaa ggtgcagccg tcaaggttaa ggctggccat atcagcgact gaaaagcggc
     3841 cagcctcggc cttgtttgac gtataaccaa agccaccggg caaccaatag cccttgtcac
     3901 ttttgatcag gtagaccgac cctgaagcgc ttttttcgta ttccataaaa cccccttctg
     3961 tgcgtgagta ctcatagtat aacaggcgtg agtaccaacg caagcactac atgctgaaat
     4021 ctggcccgcc cctgtccatg cctcgctggc ggggtgccgg tgcccgtgcc agctcggccc
     4081 gcgcaagctg gacgctgggc agacccatga ccttgctgac ggtgcgctcg atgtaatccg
     4141 cttcgtggcc gggcttgcgc tctgccagcg ctgggctggc ctcggccatg gccttgccga
     4201 tttcctcggc actgcggccc cggctggcca gcttctgcgc ggcgataaag tcgcacttgc
     4261 tgaggtcatc accgaagcgc ttgaccagcc cggccatctc gctgcggtac tcgtccagcg
     4321 ccgtgcgccg gtggcggcta agctgccgct cgggcagttc gaggctggcc agcctgcggg
     4381 ccttctcctg ctgccgctgg gcctgctcga tctgctggcc agcctgctgc accagcgccg
     4441 ggccagcggt ggcggtcttg cccttggatt cacgcagcag cacccacggc tgataaccgg
     4501 cgcgggtggt gtgcttgtcc ttgcggttgg tgaagcccgc caagcggcca tagtggcggc
     4561 tgtcggcgct ggccgggtcg gcgtcgtact cgctggccag cgtccgggca atctgccccc
     4621 gaagttcacc gcctgcggcg tcggccacct tgacccatgc ctgatagttc ttcgggctgg
     4681 tttccactac cagggcaggc tcccggccct cggctttcat gtcatccagg tcaaactcgc
     4741 tgaggtcgtc caccagcacc agaccatgcc gctcctgctc ggcgggcctg atatacacgt
     4801 cattgccctg ggcattcatc cgcttgagcc atggcgtgtt ctggagcact tcggcggctg
     4861 accattcccg gttcatcatc tggccggtgg tggcgtccct gacgccgata tcgaagcgct
     4921 cacagcccat ggccttgagc tgtcggccta tggcctgcaa agtcctgtcg ttcttcatcg
     4981 ggccaccaag cgattcccac acattatacg agccggaagc ataaagtgta aagcctagat
     5041 ccgaaggatg agccgggctg aatgatcgac cgagacaggc cctgcggggc tgcacacgcg
     5101 cccccaccct tcgggtaggg ggaaaggccg ctaaagcggc taaaagcgct ccagcgtatt
     5161 tctgcggggt ttggtgtggg gtttagcggg ctttgcccgc ctttccccct gccgcgcagc
     5221 ggtggggcgg tgtgtagcct agcgcagcga atagaccagc tatccggcct ctggccgggc
     5281 atattgggca agggcagcag cgccccacaa gggcgctgat aaccgcgcct agtggattat
     5341 tcttagataa tcatggatgg atttttccaa caccccgcca gcccccgccc ctgctgggtt
     5401 tgcaggtttg ggggcgtgac agttattgca ggggttcgtg acagttattg caggggggcg
     5461 tgacagttat tgcaggggtt cgtgacagtt agggcgcgcc cagctgtcta gggcggcgga
     5521 tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct
     5581 ttcgactgag cctttcgttt tatttgatgc ct
//
