LOCUS       40924_39578        3907 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSEVA541, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent 
            platform for analysis and deployment of complex prokaryotic 
            phenotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 3907)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3907)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ApE v. v2.0.40
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..32
                     /label=R24
                     /note="R24"
     misc_feature    56..112
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(121..144)
                     /label=F24
                     /note="F24"
     terminator      155..249
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             364..1551
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     oriT            1700..1808
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      1902..2490
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2547..3377)
                     /codon_start=1
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
                     /translation="VASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      3391..3742
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      complement(3815..3901)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc
      121 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg
      181 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
      241 ggtgagaatc caggggtccc caataattac gatttacgta tttaaatttg acagcttatc
      301 atcgataaac tgtaatgcgg tagtttatca cagttaaatt gctaacgcag tcaggcaccg
      361 tgtatgaaat ctaacaatgc gctcatcgtc atcctcggca ccgtcaccct ggatgctgta
      421 ggcataggct tggttatgcc ggtactgccg ggcctcttgc gggatatcgt ccattccgac
      481 agcatcgcca gtcactatgg cgtgctgcta gcgctatatg cgttgatgca atttctatgc
      541 gcacccgttc tcggagcact gtccgaccgc tttggccgcc gcccagtcct gctcgcttcg
      601 ctacttggag ccactatcga ctacgcgatc atggcgacca cacccgtcct gtggattctc
      661 tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat
      721 atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt
      781 ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccctg
      841 cacgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc
      901 ctaatgcagg agtcgcataa gggagagcgc cgtccgatgc ccttgagagc cttcaaccca
      961 gtcagctcct tccggtgggc gcggggcatg accattgtgg ccgcacttat gactgtcttc
     1021 tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac
     1081 cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac
     1141 gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc
     1201 attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga
     1261 ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg
     1321 ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg
     1381 ctcgcggctc ttaccagcct aacttcgatc actggaccgc tgatcgtcac ggcgatttat
     1441 gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggcgccgc cctatacctt
     1501 gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac ctgagacaat
     1561 tgtccttttc cgctgcataa ccctgcttcg gggtcattat agcgattttt tcggtatatc
     1621 catccttttt cgcacgatat acaggatttt gccaaagggt tcgtgtagac tttccttggt
     1681 gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt
     1741 ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag
     1801 gctggccgta ggccggccga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     1861 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1921 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1981 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2041 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2101 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2161 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2221 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2281 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggcat
     2341 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2401 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2461 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggccgt gaaaggcagg
     2521 ccggtccgtg gtggccacgg cctctaggcc agatccagcg gcatctgggt tagtcgagcg
     2581 cgggccgctt cccatgtctc accagggcga gcctgtttcg cgatctcagc atctgaaatc
     2641 ttcccggcct tgcgcttcgc tggggcctta cccaccgcct tggcgggctt cttcggtcca
     2701 aaactgaaca acagatgtgt gaccttgcgc ccggtctttc gctgcgccca ctccacctgt
     2761 agcgggctgt gctcgttgat ctgcgtcacg gctggatcaa gcactcgcaa cttgaagtcc
     2821 ttgatcgagg gataccggcc ttccagttga aaccactttc gcagctggtc aatttctatt
     2881 tcgcgctggc cgatgctgtc ccattgcatg agcagctcgt aaagcctgat cgcgtgggtg
     2941 ctgtccatct tggccacgtc agccaaggcg tatttggtga actgtttggt gagttccgtc
     3001 aggtacggca gcatgtcttt ggtgaacctg agttctacac ggccctcacc ctcccggtag
     3061 atgattgttt gcacccagcc ggtaatcatc acactcggtc ttttcccctt gccattgggc
     3121 tcttgggtta accggacttc ccgccgtttc aggcgcaggg ccgcttcttt gagctggttg
     3181 taggaagatt cgatagggac acccgccatc gtcgctatgt cctccgccgt cactgaatac
     3241 atcacttcat cggtgacagg ctcgctcctc ttcacctggc taatacaggc cagaacgatc
     3301 cgctgttcct gaacactgag gcgatacgcg gcctcgacca gggcattgct tttgtaaacc
     3361 attgggggtg aggccacgtt cgacattcct tgtgtataag gggacactgt atctgcgtcc
     3421 cacaatacaa caaatccgtc cctttacaac aacaaatccg tcccttctta acaacaaatc
     3481 cgtcccttaa tggcaacaaa tccgtccctt tttaaactct acaggccacg gattacgtgg
     3541 cctgtagacg tcctaaaagg tttaaaaggg aaaaggaaga aaagggtgga aacgcaaaaa
     3601 acgcaccact acgtggcccc gttggggccg catttgtgcc cctgaagggg cgggggaggc
     3661 gtctgggcaa tccccgtttt accagtcccc tatcgccgcc tgagagggcg caggaagcga
     3721 gtaatcaggg tatcgaggcg gattcaccct tggcgtccaa ccagcggcac cagcggcgcc
     3781 tgagaggggc gcgcccagct gtctagggcg gcggatttgt cctactcagg agagcgttca
     3841 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
     3901 gatgcct
//
