LOCUS       40924_39568        3909 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSEVA532, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent 
            platform for analysis and deployment of complex prokaryotic 
            phenotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 3909)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3909)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ApE v. v2.0.40
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    125..146
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        161..191
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    199..215
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     223..239
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(252..308)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(309..325)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(329..352)
                     /label=F24
                     /note="F24"
     terminator      604..698
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             813..2000
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     oriT            2149..2257
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(2271..2930)
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      complement(2931..3702)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     terminator      complement(3817..3903)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
       61 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      121 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
      181 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
      241 atgattacgc caagcttgca tgcctgcagg tcgactctag aggatccccg ggtaccgagc
      301 tcgaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      361 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      421 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
      481 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
      541 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgaactagtc
      601 ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa
      661 cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aggggtcccc aataattacg
      721 atttacgtat ttaaatttga cagcttatca tcgataaact gtaatgcggt agtttatcac
      781 agttaaattg ctaacgcagt caggcaccgt gtatgaaatc taacaatgcg ctcatcgtca
      841 tcctcggcac cgtcaccctg gatgctgtag gcataggctt ggttatgccg gtactgccgg
      901 gcctcttgcg ggatatcgtc cattccgaca gcatcgccag tcactatggc gtgctgctag
      961 cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg tccgaccgct
     1021 ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac tacgcgatca
     1081 tggcgaccac acccgtcctg tggattctct acgccggacg catcgtggcc ggcatcaccg
     1141 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
     1201 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
     1261 ccgggggact gttgggcgcc atctccctgc acgcaccatt ccttgcggcg gcggtgctca
     1321 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgcc
     1381 gtccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
     1441 ccattgtggc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
     1501 cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc
     1561 tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg
     1621 ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg
     1681 gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc
     1741 tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg
     1801 acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca
     1861 ctggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg
     1921 catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat
     1981 ggagccgggc cacctcgacc tgagacaatt gtccttttcc gctgcataac cctgcttcgg
     2041 ggtcattata gcgatttttt cggtatatcc atcctttttc gcacgatata caggattttg
     2101 ccaaagggtt cgtgtagact ttccttggtg tatccaacgg cgtcagccgg gcaggatagg
     2161 tgaagtaggc ccacccgcga gcgggtgttc cttcttcact gtcccttatt cgcacctggc
     2221 ggtgctcaac gggaatcctg ctctgcgagg ctggccgtag gccggcccta ccggcgcggc
     2281 agcgttaccc gtgtcggcgg ctccaacggc tcgccatcgt ccagaaaaca cggctcatcg
     2341 ggcatcggca ggcgctgctg cccgcgccgt tcccattcct ccgtttcggt caaggctggc
     2401 aggtctggtt ccatgcccgg aatgccgggc tggctgggcg gctcctcgcc ggggccggtc
     2461 ggtagttgct gctcgcccgg atacagggtc gggatgcggc gcaggtcgcc atgccccaac
     2521 agcgattcgt cctggtcgtc gtgatcaacc accacggcgg cactgaacac cgacaggcgc
     2581 aactggtcgc ggggctggcc ccacgccacg cggtcattga ccacgtaggc cgacacggtg
     2641 ccggggccgt tgagcttcac gacggagatc cagcgctcgg ccaccaagtc cttgactgcg
     2701 tattggaccg tccgcaaaga acgtccgatg agcttggaaa gtgtcttctg gctgaccacc
     2761 acggcgttct ggtggcccat ctgcgccacg aggtgatgca gcagcattgc cgccgtgggt
     2821 ttcctcgcaa taagcccggc ccacgcctca tgcgctttgc gttccgtttg cacccagtga
     2881 ccgggcttgt tcttggcttg aatgccgatt tctctggact gcgtggccat gcttatctcc
     2941 atgcggtagg ggtgccgcac ggttgcggca ccatgcgcaa tcagctgcaa cttttcggca
     3001 gcgcgacaac aattatgcgt tgcgtaaaag tggcagtcaa ttacagattt tctttaacct
     3061 acgcaatgag ctattgcggg gggtgccgca atgagctgtt gcgtaccccc cttttttaag
     3121 ttgttgattt ttaagtcttt cgcatttcgc cctatatcta gttctttggt gcccaaagaa
     3181 gggcacccct gcggggttcc cccacgcctt cggcgcggct ccccctccgg caaaaagtgg
     3241 cccctccggg gcttgttgat cgactgcgcg gccttcggcc ttgcccaagg tggcgctgcc
     3301 cccttggaac ccccgcactc gccgccgtga ggctcggggg gcaggcgggc gggcttcgcc
     3361 cttcgactgc ccccactcgc ataggcttgg gtcgttccag gcgcgtcaag gccaagccgc
     3421 tgcgcggtcg ctgcgcgagc cttgacccgc cttccacttg gtgtccaacc ggcaagcgaa
     3481 gcgcgcaggc cgcaggccgg aggcttttcc ccagagaaaa ttaaaaaaat tgatggggca
     3541 aggccgcagg ccgcgcagtt ggagccggtg ggtatgtggt cgaaggctgg gtagccggtg
     3601 ggcaatccct gtggtcaagc tcgtgggcag gcgcagcctg tccatcagct tgtccagcag
     3661 ggttgtccac gggccgagcg aagcgagcca gccggtggcc gctcgcggcc atcgtccaca
     3721 tatccacggg ctggcaaggg agcgcagcga ccgcgcaggg cgaagcccgg agagcaagcc
     3781 cgtagggggg gcgcgcccag ctgtctaggg cggcggattt gtcctactca ggagagcgtt
     3841 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
     3901 ttgatgcct
//
